Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish.
Pannexin 1 (Panx1) is an integral membrane glycoprotein forming ATP release channels in different tissues and cell types1,2,3,4,5, including neurons6,7,8. In the CNS, evidence for physiological functions of Panx1 points at roles in the processing of sensory signals and learning and memory9,10,11. For example, in Panx1 knockout mice, altered retinal contrast sensitivity12 and hearing loss have been found13,14. Also, performance in spatial learning and memory abilities such as object recognition and fear conditioning tasks are decreased9,15,16. Intellectual disabilities, severe hearing loss, primary ovarian failure, kyphoscoliosis, and difficulties navigating in darkness were found in the first human patient identified with a homozygous Panx1 mutation17.
To form a better view of Panx1 functions in the processing of sensory information, we used the zebrafish as model organisms. Two Panx1 genes, panx1a and panx1b, originated from partial genome duplications during early teleost evolution18,19. Although the two genes have been separated for more than 200 million years, principal channel functions are comparable to rodent or human Panx120. In the retina, the panx1a protein is expressed in horizontal cells19,20 and plays essential roles in feedback from horizontal cells to cones in adult zebrafish21,22,23. Here the panx1a gene was edited using transcription activator-like effector nucleases (TALEN). A loss of function mutation allowed to investigate Panx1a in 6 dpf old zebrafish larvae at a developmental stage when neuronal networks for visually guided locomotor behaviors were functional. Transcriptome analysis detected noteworthy expression differences of genes associated with eye development and vision-related processes. When followed up, altered dopaminergic signaling affecting both pre- and postsynaptic proteins were found. The molecular evidence was supported by measuring abnormal responsiveness of panx1a−/− larvae to darkness, or after the abrupt loss of illumination. Pharmacological activation of D1/D2-like dopamine receptors simulated this phenotype. Blocking D2-like receptors with Haloperidol rescued the phenotype. In vivo electrophysiological recording of local field potentials (LOF) from the larval optic tectum in a region receiving input from the retina demonstrated that the dynamic transition from low to higher-frequency brain waves in light and darkness was compromised in panx1a−/− larvae. This phenotype was reproduced by pharmacological blocking of Panx1a, or by treatment with the D1/D2 receptor agonist apomorphine. This research delivers a novel association between Panx1a in the integration of sensory-motor behavior through modulation of dopaminergic signaling.
Targeted ablation of panx1a
The panx1a mutant allele was generated by TALEN-mediated mutagenesis targeting the single AfeI restriction endonuclease recognition site in the fourth exon of panx1a (Fig. 1a). Three doses of 30, 60, and 100 pg/TALEN cRNA pair were injected into 1-cell stage embryos of the TL strain. 61% and 38.6% of all F0 embryos developed typically after injection of 30 pg/nl and 60 pg/nl cRNA, whereas 90% of the embryos developed malformations or died within 24hrs after injection of 100 pg/nl (Fig. 1b). A concentration of 30 pg/nl cRNA was used in follow-up gene-targeting experiments. The restriction fragment length polymorphism test (RLFP RFLP) of ten randomly selected embryos revealed a mutagenesis efficiency of ≈50%, as evidenced by a partial loss of the restriction enzyme recognition site at the TALENs cut sites (Fig. 1c). DNA sequence analysis of multiple microinjected embryos confirmed the efficient introduction of short, 4 to 7 long nucleotide deletions in the panx1a exon 4 (Fig. 1d). A founder fish carrying a four bp deletion (panx1aΔ4) was selected for further experimentation. The four base pair deletion caused a frameshift at amino acid 195, resulting in a premature stop codon leading to truncated 201-amino-acid protein, lacking most of the 416 amino acid long Panx1a protein sequence including two transmembrane regions and the entire carboxyterminal domain (Fig. 1e). After transfection into mouse Neuroblastoma 2a (Neuro2a) cells the subcellular localization of the truncated Panx1aΔ4-EGFP protein had a diffuse cytoplasmic signal suggesting that the mutant protein was unable to traffic efficiently to the cell membrane (Fig. 1f), and unlikely to form functional channels (Fig. 1f, left panel). The full-length Panx1awt-EGFP protein was detectable in the plasma membrane (Fig. 1f, right panel) in line with previous reports20,24.
Characterization of panx1a −/− larvae
A comparison of panx1a−/− and wild type TL larvae (F3 generation) revealed no gross anatomical defects (Fig. 2a), and juvenile fish were visually indistinguishable from age-matched TL siblings. Adult panx1a−/− zebrafish were viable and fertile like the parental TL strain, or heterozygous fish, suggesting that developmental differences were restricted to early developmental stages. The panx1a mRNA level was significantly reduced in 6 dpf panx1a−/− larvae indicating small deletion-mediated RNA decay (Fig. 2b). Compensatory regulation of three other pannexin genes similar to previous reports using Panx1−/− mouse models was excluded25. Panx1b mRNA was expressed at equal levels in both wild-type and panx1a−/−. A low level of panx3 mRNA expression was detected, but not panx2 mRNA.
Immunohistochemistry (IHC) experiments confirmed a reduction of Panx1a expression in the retina of adult zebrafish. PSD-95 staining of cone photoreceptor terminals was similar in both genotypes. No gross anatomical alteration of the retina was noticeable. The immunoreactivity found in the outer retina of panx1a+/+ controls was reduced in age-matched knock-out retina (Fig. 2c). Panx1a expression in the outer retina of 3 dpf larvae TL larvae resembled the localization found in the adult retina (see Supplementary Fig. S1a). The Panx1a immunoreactivity was reduced in Panx1a−/− larvae (Supplementary Fig. S1b). Residual immunoreactivity found in both adult and 3 dpf retina was attributed to cross-reactivity of the antibody with the panx1b protein, which shares 66% amino acid identity with panx1a in the carboxyterminal domain.
Transcriptome profiling of 6 dpf panx1a −/− larvae
The transcriptomes of panx1a−/− and wild-type TL larvae were compared at 6 dpf (NCBI Gene Expression Omnibus (GEO) database; data deposit GSE147068;). A total of 12302 RNAs was found in each of the six samples (TL control, n = 3; panx1a−/−, n = 3) sequenced. In panx1a−/− larva, 1902 RNAs were downregulated, and 933 RNAs upregulated when the cutoff for the false discovery rate (FDR) was set to 0.05, and the significance of regulation was defined as p-value < 0.0001. Gene-specific expression information retrieved from the Zebrafish Information Network (ZFIN) database allowed categorizing the representation of regulated genes to those previously described as expressed in the central nervous system (CNS) or visual system. Three hundred seventy-seven upregulated genes and 646 downregulated genes matched genes expressed in the central nervous system (Fig. 3a, left panel). Furthermore, 317 upregulated genes and 473 downregulated genes matched genes expressed in the visual system (Fig. 3a, right panel).
The AmiGO2 analysis (http://amigo.geneontology.org/amigo/landing) revealed enriched Gene Ontology (GO) terms and pathways (Supplementary Tables S1a and S1b). The most significant enriched Reactome processes (cut-off: p-value < 0.001; fold change of expression >2.5) (Fig. 3b) represented the phototransduction cascade, as well as the processing of mRNAs (Fig. 3b. left plot, upregulated genes). Processes such as development and cell signaling through membrane receptors represented downregulated genes (Fig. 3b, right plot).
RNAseq data (cutoff: p-value < 0.0001; FDR 0.05) were also validated by RNA-qPCR (cutoff: p-value < 0.05). This experiment confirmed that targeting panx1a upregulated mRNAs specific for phototransduction and the development of the visual system (Fig. 3c, Supplementary Table S2). Selected purinergic receptors and genes representing voltage-gated ion channel families were confirmed as downregulated in panx1a−/− larva (Fig. 3c, Supplementary Table S2). We concluded that the genetic ablation of panx1a in the zebrafish caused a molecular phenotype in which both the visual system and central nervous system was affected in 6 dpf old larvae.
Loss of panx1a impairs locomotion in the dark
Visually guided behavior was investigated after transcriptome analysis suggested changes to visual functions. At 6 dpf, both genotypes, panx1a+/+ and panx1a−/−, swam longer distances and more rapidly during the Light-ON phase than during the Light-OFF phase when baseline swimming activity of unrestrained larvae was tested. In light, both genotypes showed no significant difference in the average distance traveled (t = 1.86, df = 133.52, p-value = 0.06531; n = 60) and average velocity (t = 1.03, df = 133.74, p-value = 0.3065; n = 60) (Fig. 4a). A representative example illustrates the preference of larvae for swimming close to the circumferences of the wells, with spontaneous crossings of the central zone. During the dark phase, panx1a−/− larvae were less active and showed intermittent episodes of swimming bouts with slow and medium speeds. Panx1a−/− larvae swam significantly less (t = −3.08, df = 135.9, p-value = 0.002; n = 70) and with lower velocity (t = −2.79, df = 131.73, p-value = 0.006; n = 70) compared to TL-controls (Fig. 4b). Furthermore, panx1a−/− larvae avoided crossing the central zone of the well in the dark.
Visual-motor response (VMR) in panx1a −/− larvae
The visual-motor response (VMR) assay measured the responsiveness of zebrafish larvae to light changes. The orthogonal transformation of the multidimensional VMR data by principal component analysis (PCA) was used to identify the most relevant of seven behavioral parameters. PC1 and PC2 captured more than 76% and 21% of the data variance (Fig. 5a). Two-dimensional PCA plotting confirmed that both genotypes were separated into two distinct clusters (Fig. 5b). The variable correlation plot allowed to conclude that the total activity duration (TAD; in red) had the most significant contribution to the variability in PC1 (Fig. 5c). Thus, TAD was chosen to determine the differences between genotypes in VMR assays.
TL control and panx1a−/− larvae showed distinct VMR, as demonstrated by the corresponding average TAD plots in an experimental paradigm of 3 cycles of alternating Light-ON and Light-OFF periods (Fig. 5d; top panel). In all cycles, the larval activity was consistently reduced in the absence of Panx1a (Fig. 5d). In the 1 min period before the Light-ON stimulus (−60-0 s), panx1a−/− larvae showed a significantly lower activity level than TL controls (p-value = 0.015). At Light-ON (0–1 sec), both TL controls and mutant larvae augmented their activity immediately; only the peak response to the light stimulus was trending lower in panx1a−/− larvae compared to TL controls (p-value = 0.382). During the subsequent light period, both mutants and TL controls gradually returned to the baseline activity level (in 1–60 sec, p-value = 0.876; Fig. 5e).
Notably, at Light-OFF, the mutants responded differently to control TL larvae. For the 1 min period before Light-OFF stimulus (−60-0 s), panx1a−/− larvae had lower baseline activity (p-value = 0.036). Following the abrupt loss of illumination (0–1 s), TL control larvae initially increased their locomotor activity and then remain more active for 10 ± 2 minutes. After that, the magnitude and duration of swim bouts gradually returned to baseline activity level. In contrast, panx1a−/− larvae demonstrated a significant reduction (p-value = 0.001) of dark stimulus-response and continued to show a reduced activity during the dark period. This observation was supported by the corresponding TAD plots (Fig. 5f), in which the activity of mutants after the light change was noticeably lower when compared to TL controls (p-value = 0.0249). These results indicated that the panx1a mutant fish readily detected the Light-ON stimulus but responded weakly to the Light-OFF condition. Therefore, Panx1a functions appear important for the detection of light decrement and the corresponding behavioral response in larval zebrafish.
Alterations of the Dopamine (DA) signaling pathway in panx1a mutants
The role of dopaminergic signaling was investigated based on the differential expression of dopamine receptors found by RNA-seq and altered locomotion in the dark. These observations suggested that loss of Panx1a function in knock-out larvae could have affected expression of genes involved in dopamine signaling. This idea was tested first by RT-qPCR. Among transcripts of dopaminergic genes located in the presynaptic compartment, a significant up-regulation of the tyrosine hydroxylase gene, which catalyzes the rate-limiting step in DA synthesis, was detected when cut-off criteria for the expression ratio was >1.5, and the p-value < 0.05. Other prominent regulated transcripts were slc6a3/dat, a sodium-dependent dopamine transporter, and slc18a2/vmat2, an ATP-dependent vesicular monoamine transporter involved in the dopamine neurotransmitter release cycle (Fig. 6a; Supplementary Table S3). Dopamine receptor genes significantly upregulated in panx1−/− larvae represented both D1/D2 subfamilies (Fig. 6a; Supplementary Table S3). A three-fold upregulation of the tyrosine hydroxylase protein in panx1−/− larvae (Fig. 6b,c; n = 4; significance p-value < 0.01), and a significantly higher Th-positive cell count in the retina of panx1a−/− larvae (Fig. 6d; panx1a+/+, n = 7; panx1a−/−, n = 6; p-value = 0.034; Supplementary Fig. S1c–e) complemented the RT-qPCR result.
Apomorphine (Apo), a non-specific dopamine receptors agonist, was used to investigate the overall role of augmented dopamine signaling in panx1a−/− and control larvae. Dose-response assays confirmed the known U-shape relationship between Apo concentrations and larval activity in the light and dark (Supplementary Fig. S2a and S2b)26,27. A concentration of 50 µM Apo, representing the most substantial effect, was selected. The larval activity during the second trial was analyzed (i.e., 90–120 min), when the maximal effect of Apo was reached.
Figure 7a exemplifies larvae of both genotypes, which appeared to be hyperactive in the dark period preceding the Light-ON (i.e., −60 to 0 sec). Though, the increment of activity was significant only in mutants. Also, Apo treatment affected the Light-ON peak response (0 to 1 sec period) similarly in both groups. It significantly raised the magnitude of the Light-ON peak response, so no further difference was detected between groups (All the p-values are given in Table 1).
When the larval activity was analyzed during the second Light-OFF trail, differing patterns of effects were observed in the two groups. In the light period preceding dark (i.e., −60 to 0 sec), Apo (50 µM) markedly decreased activity in TL controls but did not significantly alter that in panx1a−/− larvae (Fig. 7b). At the light offset (0 to 1 sec), similar behaviors occurred. Apo abolished the larval peak response to the sudden darkness in TL controls to the point that it resembled a mutant response. No significant difference was observed in the panx1a−/− larvae Light-OFF peak response (Fig. 7b; All the p-values are given in Table 1).
The mean activity data suggested that changes in the lighting conditions affected the levels of activity of larvae given 50 µM Apo (Fig. 7a,b). While Apo treatment enhanced larval reaction to the sudden illumination (Light-ON) regardless of their genotypes, it suppressed larval response to the sudden darkness (Light-OFF) only in panx1a+/+.
Stimulation of D1/D2 dopaminergic signaling with Apo induced in panx1a+/+ larvae a panx1a−/− like Light-OFF response. This result suggested a causal relationship between Panx1a function, augmentation of dopaminergic signaling, and the described behavioral phenotype. The class of dopamine receptor which modulated the Light-OFF component of the VMR was identified using D1- and D2-like dopamine receptor antagonists. Treatment with the D2-like receptor antagonist Haloperidol (20 μM) rescued the dopamine-induced Light-OFF response deficiency in mutants (n = 64; p-value < 0.001) (Table 2), with no significant effects in wild-type controls (n = 64; p-value = 0.27) (Fig. 7c). The D1-like receptor antagonist SCH-23390 (20 μM) did not affect the mutants’ peak response (n = 40; p-value = 0.76) and the Light-OFF response in wild-type control larvae (n = 40, p-value = 0.218) (Fig. 7d). A comparison of TAD at Light-OFF across different treatments showed that Haloperidol effectively rescued the Light-OFF phenotype of panx1a−/− larvae (Fig. 7e). The results suggested a participation of D2-like receptors in dopamine-induced Light-OFF response deficiency observed in panx1a−/− zebrafish larvae.
Loss of panx1a modifies brain wave frequency transitions in response to light
Changes to retinotectal circuitry were tested by recording local field potentials (LOF) in the optic tectum of immobilized 6 dpf larvae exposed to full-field light stimuli (Fig. 8a). In wild-type larvae, the peak in normalized power shifted from low frequencies (less than 10 Hz) to gamma frequencies (35–40 Hz) when the light was shut off, and larvae were exposed to darkness for 10 minutes (Fig. 8b). This response was abolished in panx1a−/− larvae. Shifting from dark to light conditions gave identical results in peak power (Fig. 8b). Loss of Panx1a, as well as treatment of wild type larvae with 100 μM probenecid, an established blocker for pannexins, significantly reduced the changes in normalized power for both the low and gamma frequency ranges compared to control TL larvae (Fig. 8c, Table 3). Further, the treatment of wild-type larvae with 50μM apomorphine, the D1R/D2R agonist, created a significant change to transitions between low and gamma frequencies under light stimulus when compared to controls (Fig. 8c, Table 3). Our results demonstrated that targeting Panx1a, either with pharmacology or by gene-editing, or targeting dopaminergic synapses, caused a significant change in properties of the retinotectal pathway.
Ablation of the panx1a gene caused profound differences of the transcriptome, visual-motor behavior, as well as vision-related neuronal network properties in the optic tectum of 6 dpf zebrafish larvae. The employed targeted gene knockout approach, transcription activator-like effector nucleases (TALEN), introduced a small deletion in exon4 of the panx1a gene. This mutation caused the generation of a truncated protein. The mutation was distinct from the previously reported panx1a−/− model in which the start codon was targeted23, but better compared to the mouse knockout model with deletions of exons3 and 4, which was used previously to investigate sensory processes and learning and memory9,28. Similar to other rodents29,30 and the other zebrafish model23, the panx1a−/− zebrafish are viable and fertile, with no apparent anatomical abnormalities or morphologic changes to the eyes.
All investigations were performed at 6 dpf when the subdivisions of the brain and sensory systems have formed, and excitatory glutamatergic neurons, inhibitory GABAergic interneurons, astrocytes, and microglia required for normal excitatory discharges within neuronal networks are functional31,32,33. The transcriptome analysis demonstrated that loss of panx1a effectively altered transcription of almost 20% of all expressed genes. The observed expression regulation was consistent with the known expression profile of panx1a across multiple organ systems18,19,20. Vision-related processes and the development of the eye, or the transportome, a collective term referring to proteins facilitating transport to and across membranes, which includes ion channels, purinergic and neurotransmitter receptors, solute carrier (SLC) transporters were affected34. These transcriptional changes are noteworthy and substantiate the role of panx1a as a significant molecular hub, which was proposed previously from interactome studies35. Moving forward the transcriptome study opens windows of opportunity to study the diverse mechanism dynamically regulating Panx1 channel function, such as elevated extracellular K+36, or the interaction with purinergic37,38, NMDA39,40, or α1-adrenergic receptors41,42.
The regulation of genes known as primarily located in the pre- and postsynaptic compartments of dopaminergic synapses provide for the first-time evidence that ablation of Panx1a alters dopaminergic signaling and that this change was consequential for behavioral outcomes. Cellular effects driven by the loss of Panx1a functions reduced the navigational competence of larvae in a dark environment, as well as response to the sudden darkness. This response was reproduced in wild type larvae through pharmacological activation of D1-/D2-like dopaminergic receptors and rescued in mutants by treatment with the D2-like receptor antagonist Haloperidol. The visual-motor behavioral assay (VMR) allowed measuring the responsiveness of zebrafish larvae to changes in light intensities43. This responsiveness requires an intact retina44, and photoreceptor populations with distinct spectral properties45. The advantage of this test compared to the optokinetic reflex test (OKR) is that it allows extracting behavioral responses caused by changes in the retinal ON and OFF pathways. A limitation of the VMR test is that at 6 dpf it does not discriminate between rod and cone responses46 since rod photoreceptors are not functional in zebrafish until 15 dpf 47,48. Altogether, we propose that the altered behavior of panx1a−/− larvae in a dark environment and during light/dark transitions is caused by Panx1a modulating visual inputs through D2-like dopaminergic receptors signaling. In line with this proposal is that a recent study reported Panx1 channel activity currents only in the OFF-type retinal ganglion cells (RGCs)28, the neurons responsible for detecting light decrement. Beyond the visual system, a recent behavioral study in Panx1 knock out mice showed that loss of Panx1 induces difficulties in motor control and changes of the sleep-wakefulness cycle. The authors suggested deficiencies in adenosine and other signaling pathways were causing the reported deficiency49.
Local field potentials were recorded in vivo from superficial layers of the optic tectum (OT) to capture changes caused by Panx1a modulating visual inputs. Using full-field Light-ON and Light-OFF stimuli, we demonstrated that the neuronal circuitry of 6 dpf larvae responded to light changes with a flexible transition through various brain wave frequencies. This transition is altered in panx1a−/− larvae, and reproduced by pharmacological blocking of Panx1a, or by treatment with the D1/D2 receptor agonist apomorphine. This result suggests that Panx1a affects the role of the optic tectum in modulating the processing of sensory information received from inputs from the retina and that dopaminergic signaling plays a role in this change. This conclusion is consistent with the role of the OT in constructing an image of the physical surroundings, integrating visually acquired information with motor inputs and outputs to initiate appropriate behavioral responses or changes thereof as quantified in this study.
At the level of detail provided here, no simple conclusion can be offered, which explains the full spectrum of differences caused by the ablation of the panx1a gene. We speculate that the phenotype is initiated by impaired levels of ATP and adenosine. The results shown here advocate for a role of Panx1a in the developmental plasticity of the visual system in a way similar to the plasticity of cortical neurons in Panx1−/− mice50. In the retina, from 48 h post-fertilization, RGCs begin to connect with the neuropil of the optic tectum and by 3 dpf retinal stratification is well established51. At 3 dpf the optokinetic response is already established, by 5 dpf larvae are capable of tracking and capturing prey, and by 8 dpf the optic tectum is structurally and functionally relatively mature52. The size of the visual receptive fields increases from 4 dpf to 6 dpf and then refines to the same size as that at 4 dpf by 8–9 dpf53. During this critical developmental period, excitatory components start dominating while GABAergic responses most likely switch from depolarizing to hyperpolarizing currents, making functional pruning of feedforward inputs most likely the most critical factor in receptive field refinement. Input to the optic tectum derives from the retina, striatum, or hypothalamus54,55. The superficial layers recorded from in this study receive input from the retina55. They are distinct from dopaminergic input from the striatum and the hypothalamus connecting to deep layers of the optic tectum. In the retina, dopamine has long been known to play important modulatory roles in the vertebrate visual system56,57,58,59. Panx1a in the zebrafish retina is located in HCs-cones synaptic complexes20, in proximity to TH expressing neurons19, where different neurotransmitters like ATP and dopamine act to fine-tune the conductance between cone and HCs. In the darkness, a steady inflow of cations keeps photoreceptors relatively depolarized. Glutamate is released continuously from cones in the synaptic space between cones and HCs. Dopamine increases the conductance of the glutamatergic synapse between cones and HCs through a D1R-mediated mechanism60, leading to depolarization of HCs57,61. Noteworthy, when HCs are depolarized, in the dark, the Panx1 channel is maximally active and releases ATP into the synaptic cleft, by means of which it participates in sending inhibitory feedback to cones22,23. The negative feedback from HCs to cone photoreceptors generates the center/surround organization of bipolar cell receptive fields and is crucial for visual phenomena like contrast enhancement and redundancy reduction23.
The vision centric investigation of molecular, electrophysiological, and behavioral properties of panx1a−/− zebrafish larvae has uncovered a novel association between Panx1a in the integration of sensory-motor behavior through modulation of dopaminergic signaling. We acknowledge that beyond the scope of this study, Panx1a could also play other roles in the nervous system, perhaps in neural plasticity transcending a range of spatio-temporal scales which serve non-sensory functions in the motor system, or cognitive functions, such as affecting learning, and memory in mice9. The panx1a−/− model will provide a versatile platform for future investigation of these knowledge gaps using genetic, pharmacological, and behavioral phenotyping.
Fish Husbandry and embryo collection
Zebrafish (Danio rerio) of strain Tupfel long fin (TL) were used to generate the panx1a−/− mutant line62. The fish were maintained in a recirculation system (Aquaneering Inc., San Diego, CA) at 28 °C on a 14 hours light/ 10 hours dark cycle. Experiments and procedures with animals were performed at York Universities zebrafish vivarium, according to the CACC guidelines of the Canadian Council for Animal Care (CCAC) after approval of the protocol by the Animal Care Committee (ACC) (GZ#2014-19 (R3). The number of experiments, including zebrafish larvae, was kept to the necessary minimum. The York University Biosafety Committee (YUBC) approved all other experiments (Permit#04-11).
Generating panx1a mutant by TALENs
Potential TALENs target sites on panx1a (NM_200916.1) were identified using the Mojo Hand software (http://talendesign.org)63. Sequence-specific TALEN constructs were assembled using Golden Gate cloning methods64,65. A detailed description of the design, preparation, and activity screening of TALENs, as well as microinjection procedure and validation of knockout lines, are provided in the Supplementary Information.
RNA extraction and RT-qPCR
Total RNAs were extracted from 6 dpf larvae using RNeasy Plus Mini Kit (Qiagen). The iScript Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, Canada) was used to reverse transcribe 1 µg of total RNA. The cDNA equivalent of 15 ng total RNA was analyzed in triplicate by quantitative Real Time-PCR using the SsoAdvanced SybrGreen PCR mix (Bio-Rad). All experiments included a melt curve analysis of PCR amplicons generated in each reaction. Raw cycle threshold values (Ct-values) were exported from the CFX Manager Software (Bio-Rad, Canada), and the relative gene expression was calculated using the Relative Expression Software Tool (REST-2009)66. Gene information and primer sequences are listed in Supplementary Table S4.
The RNA library preparation was performed following the NEB NEBNext Ultra II Directional RNA Library Preparation protocol (New England Biolabs Inc., Ipswich, MA, USA). RNA libraries were loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies) to check for size, quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Pooled libraries were paired-end sequenced on a High Throughput Run Mode flow cell with the V4 sequencing chemistry on an Illumina HiSeq. 2500 platform (Illumina, Inc., San Diego, CA) following Illumina’s recommended protocol to generate paired-end reads of 126-bases in length. The post-sequencing processing to final read counts, normalization, and differential gene expression analysis used multiple software packages, including a two-condition differential expression analysis using the edgeR R-package, v.3.8.6 (http://www.bioconductor.org/packages/release/bioc/html/edgeR.html)67,68.
The Zebrabox behavior system and the ZebraLab analytical suite was used for automated observation and video tracking of 6 dpf zebrafish larvae in 48-well plates (ViewPoint Life Technology, Lyon, France; http://www.viewpoint.fr). The visual-motor response (VMR) assay was based on the configurations established elsewhere43. Typically, the activities of 48 larvae were recorded simultaneously. Data were collected from video frames at 1-second intervals. For VMR, we analyzed the period from 1 min before the light change to 1 min after the light change (−60-0 sec; 0–1 sec; 1–60 sec) to capture the larval response to abrupt light change. Both assays are described in detail in the online Supplementary Information. All experiments were performed between 12:30 to 4 pm, as larvae (6 dpf) activity was previously reported to reach a stable level by early afternoon69.
Published procedures were used to prepare anesthetized zebrafish larvae (6 dpf) for in vivo electrophysiology70. Microelectrodes were back loaded with 2 M NaCl, and local field potentials were recorded using an Axiocamp 700B amplifier (Axon Instruments, San Jose, CA, USA). Typically, microelectrodes had a resistance of 2–7 M. They were placed into the optic tectum. Recordings were low-pass filtered at 1 kHz (-3 dB; eight-pole Bessel), high-pass filtered at 0.1–0.2 Hz, digitized 10 kHz using a Digidata 1550 A A/D interface, and stored on a PC computer running pClamp11 software (Axon). The basal activity was recorded for 10 minutes under LIGHT-on Light-ON conditions. The basal activity during LIGHT-off Light-OFF was recorded for another 10 minutes. In some experiment’s zebrafish larvae were pre-incubated for 1 hr with 100 µM Probenecid. During recordings, probenecid was present in the bath solution.
All the chemicals were purchased from Sigma-Aldrich (Mississauga, Canada): R-(−)-apomorphine hydrochloride hemihydrate (cat#A4393), Probenecid (cat#P8761), Pancuromium bromide (cat#P1918), Ethyl3-aminobenzoate methanesulfonate (MS-222; A5040), R(+)-SCH-23390 hydrochloride (cat#D054), and Haloperidol (cat#H1512).
Unless otherwise stated, all statistical analyses were performed in R software version 3.4.0 (http://www.r-project.org), and results are represented as the mean ± standard error of the mean (SEM) of 3 repeated experiments. A p-value < 0.05 was considered statistically significant. The average values for protein assays, cell count, electrophysiology data, traveled distance and velocity, were compared between two groups using Student’s t-test (with equal variance) or Welch’s t-test (without equal variance) as indicated. Details of procedures and software packages used can be found in the Supplementary Information section.
RNA-seq expression data have been deposited at the NCBI Gene Expression Omnibus (GEO) database (GSE147068).
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The authors would like to thank Drs. Stephen C. Ekker and Karl J. Clark (Mayo Clinic, Rochester, MN, USA) for generating the TALEN constructs. We would like to thank members of the Center for Applied Genomics (SickKids, Toronto, Canada) for the RNA-seq service. Special thanks to Janet Fleites-Medina for animal husbandry. This work was funded by a Natural Sciences and Engineering Research Council (NSERC) – Discovery Grant (GZ) and a Brain Canada Platform grant (GZ).
The authors declare no competing interests.
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Safarian, N., Whyte-Fagundes, P., Zoidl, C. et al. Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling. Sci Rep 10, 9538 (2020). https://doi.org/10.1038/s41598-020-66378-y