Abstract
The final stage of leaf ontogenesis is represented by senescence, a highly regulated process driven by a sequential cellular breakdown involving, as the first step, chloroplast dismantling with consequent reduction of photosynthetic efficiency. Different processes, such as pigment accumulation, could protect the vulnerable photosynthetic apparatus of senescent leaves. Although several studies have produced transcriptomic data on foliar senescence, just few works have attempted to explain differences in red and green leaves throughout ontogenesis. In this work, a transcriptomic approach was used on green and red leaves of Prunus cerasifera to unveil molecular differences from leaf maturity to senescence. Our analysis revealed a higher gene regulation in red leaves compared to green ones, during leaf transition. Most of the observed DEGs were shared and involved in transcription factor activities, senescing processes and cell wall remodelling. Significant differences were detected in cellular functions: genes related to photosystem I and II were highly down-regulated in the green genotype, whereas transcripts involved in flavonoid biosynthesis, such as UDP glucose-flavonoid-3-O-glucosyltransferase (UFGT) were exclusively up-regulated in red leaves. In addition, cellular functions involved in stress response (glutathione-S-transferase, Pathogen-Related) and sugar metabolism, such as three threalose-6-phosphate synthases, were activated in senescent red leaves. In conclusion, data suggests that P. cerasifera red genotypes can regulate a set of genes and molecular mechanisms that cope with senescence, promoting more advantages during leaf ontogenesis than compared to the green ones.
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Introduction
A fully developed and functional photosynthetic apparatus is the most important prerequisite for plant life, ensuring CO2 assimilation and synthesis of molecules involved in the growth of the whole plant. Senescence represents the final stage of leaf development by a highly regulated process of ontogenesis preceding cell death and during which the sequential breakdown of cellular components occurs. Leaf senescence is a biological transition, which requires complex biological processes such as a range of degradative, biosynthetic and regulatory mechanisms coordinated by changes in gene expression aimed to remobilise and reutilise molecules until the progress of senescence culminates in tissue death1,2,3,4,5.
Senescence is accompanied by visual changes in leaf pigmentation6, with a preferential degradation of chlorophylls compared to carotenoids and, in some plant species, by the synthesis of red-coloured pigments like anthocyanins4,7,8. Strong decreases in chlorophyll content and disassembling and degradation of the photosynthetic apparatus (chlorophyll and chloroplast protein degradation) is due to a regulated activity of specific enzymes and proteases that ultimately induces a decrease in photosynthetic CO2 assimilation2,6,9,10. Consequently, senescent leaves are potentially exposed to an excess of excitation energy due to a reduction in the utilisation of NADPH and ATP. This event induces an increase in the production of reactive oxygen species (ROS)11,12 that are not counterbalanced by the action of antioxidant systems13. On the other hand, ROS accumulation induces an oxidative burst (i.e. oxidation of pigments, proteins and lipids), a process necessary for photosynthetic protein degradation and nutrient remobilisation14. Levels of ROS are controlled until the end of the remobilisation and the late phase of senescence that occurs with cell death15,16. For this reason, during chloroplast senescence, dismantling has to be finely regulated and photoprotection of the photosynthetic apparatus represents a key feature in the control of the progression of leaf senescence16. Concerning the regulation of metabolic network during ontogenesis, overall sugar content increases, while amino acid concentration declines in senescing leaves1,17. Interestingly, sugars have also been recognised as regulatory molecules involved in signalling function, translation and protein stability17. Sugars, especially hexose, are able to activate several cellular functions and transcription factors involved in typical senescence-associated genes such as SAG1218; Over-expression of genes involved in sugar translocation, such as those encoding invertases, can also delay senescing processes19. In addition, a finely tuned up-regulation of anthocyanin biosynthesis promoted by sugar accumulation has been clearly demonstrated20.
Many plant species show genotypes with different constitutive leaf pigmentation, e.g. Prunus cerasifera genotypes with permanent red or green leaves. In genotypes with red leaves, the red colour is associated with the production of anthocyanins, a soluble pigment class10,21. Anthocyanins confer an enhanced photoprotection from excess solar irradiation in several plant species16,22, as already has been observed in P. cerasifera, Jathropha curcas, Juglans regia and Ocimum basilicum10,23,24,25. When studying ontogenetic differences between green and anthocyanin-rich leaves, the ideal strategy is using isogenic lines for this character, in order to exclude differences related to the genetic background. Nevertheless, this would require a number of backcrosses and would be very time-consuming because the length of the life cycle of a tree species. For this reason, several studies compare two cultivars of the same species differing in leaf colour phenotype10. Other differences between red and green leaves have been detected for leaf thickness21,26, wax formation, flavonoid production, sugar translocation10,27 and transcription factor activities of MYB and WD40 families23,25.
During the last decades, differences between genotypes with green or red leaves have been widely studied at morphological, physiological and genetic levels, and several works have concerned differences throughout ontogenesis at the physiological and biochemical levels10,28,29, but not at the gene expression level.
Next-generation sequencing (NGS) and whole-transcriptome sequencing (RNA-seq) have become powerful tools to study gene expression in both model and non-model species. To date, studies have focused on many physiological aspects of annual and perennials species including development phase transition studies5,30,31,32,33. Recently, RNA-Seq technology was applied on two cultivars of sweet basil that differed in leaf colour, providing genomic resources for the functional role of anthocyanins in photoprotection27.
In this light, gene expression analysis and changes in global gene expression patterns during leaf senescence between green and red morphs add pivotal information about cell functions and metabolism alterations that underline the developmental transition from a photosynthetic active leaf to a senescing organ, which is a source of mobilisable nutrients before the breakdown of chloroplasts.
Prunus cerasifera is a fast-growing tree appreciated worldwide as ornamental species for its fruit production and as a rootstock for fruit trees34,35. This species usually grows in several regions, spanning from the Caucasian Mountains to central Asia35,36, showing genetic and morphological differences and counting more than 20 varieties35. From a genomic point of view, P. cerasifera is a diploid species with n = 8, but with different ploidy levels amongst cultivars35,36,37. Morphologically, P. cerasifera can have different phenotypes, as in the case of ‘Pissardii’ variety, which displays reddish rather than green leaves. The molecular mechanisms of leaf senescence have been well studied in model plants as well as in major food crops; however, currently research on leaf senescence in Prunus species is still limited. As a genetically controlled development process, leaf senescence can be regulated by gene mutations, genetic transformation and molecular marker assisted breeding; nevertheless, there is still a long way to go to elucidate its complex mechanisms.
Here, for the first time, leaf transcriptome analyses were carried out on permanently green (clone ‘29C’) and red (var. ‘Pissardii’) leaves of P. cerasifera and comparing these morphs at two stages, i.e. mature and early senescent leaves. The aim of our study was to explore differences at the molecular level that could explain possible advantages to coping with senescence between green and anthocyanin-rich leaves of Prunus. The accumulation of epidermal anthocyanin can indeed effectively sunscreen the P. cerasifera var. ‘Pissardii’ leaves reducing the light which burdens to chloroplast, therefore being beneficial in terms of photoprotection compared to anthocyanin-less leaves. In fact, it has been proposed that these pigments may act as an efficient sunscreen, protecting the leaf from excessive light radiation of green-yellow wavelengths of the solar spectrum and preventing photosynthesis inhibition during leaf senescence7,8,10. In this study, we identified genes and cellular functions involved in pigment formation, photosynthetic process, sugar metabolism and stress response that differentially regulate green and red leaves throughout leaf aging. Our dataset represents a useful tool for further studies aimed at assessing the eco-physiological role of red-pigmented senescing leaves. In this light, the study of molecular components and their functionality could represent a key to the development of hypotheses concerning the organisation and regulation of metabolic networks during leaf senescence.
Results
cDNA sequencing and aligning on reference transcriptome
A total of 394,782,702 single-end reads each of 75 bp from twelve libraries of P. cerasifera red (R) and green (G) leaf morphs at two time points of ontogenesis (M, S) were collected (Table. 1). The number of trimmed reads (length = 60 bp) per library spanned from 16,280,829 to 32,776,054, enough to establish a differential expression analysis38. After trimming, high quality reads were aligned on the reference transcriptome of P. persica, a closely related species that has been often used as reference in other RNA-seq studies on Prunus species39,40; on average, 84.23% of reads per sample successfully mapped on the reference transcripts (Table. 1).
Illumina reads from green and red mature and senescent leaves of P. cerasifera were deposited on SRA under bio project accession PRJNA593381.
Analysis on differentially expressed genes of red and green P. cerasifera senescent leaves
After filtering, 19,496 expressed transcripts with RPKM > 1 in at least one library were kept and corresponding expression values were statistically tested in order to detect DEGs of red and green leaves during senescence. Overall, we detected 3,070 DEGs during green leaf ontogenesis, of which 1,654 were over-expressed (OE), and 1,416 were under-expressed (UE). Concerning the red genotype, 4,925 DEGs were retrieved with 2,483 and 2,442 over- and under-regulated genes, respectively (Fig. 1; Supplementary Table. 1).
Comparison between OE genes of the two morphs during ontogenesis showed that DEGs were mostly shared, except for 406 and 1,235 transcripts that were specifically activated by green and red leaves during senescence, respectively (Fig. 1; Supplementary Table. 1). Considering the genes showing the 15 highest fold changes in expression, among DEGs activated in green morphs, we identified transcripts related to transcription factor activity (encoding a “WRKY family protein”) and in cell wall remodelling (encoding one “laccase”). Concerning DEGs with the highest over-expression fold change in red leaves, many were involved in cell metabolism (encoding three “beta-glucosidases” and one “invertase”) and in cell stress prevention such as one encoding a “cytochrome P450 oxidase” (Supplementary Table. 1).
Regarding UE genes, the comparison between morphs showed that green leaves turned off 399 genes specifically during senescence, whereas 1,426 transcripts were specifically down-regulated in red leaves (Fig. 1). Concerning genes showing the highest fold changes during ontogenesis, in green morphs they were mostly related to transcription factor activity (encoding “MYB domain protein” and “bHLH DNA binding domain protein”). Highly under-expressed DEGs of the red morph were mainly involved in metabolic processes (e.g. three genes encoding “GDSL-like lipases”). A complete set of DEGs in green and red leaves of P. cerasifera during ontogenesis is shown in Supplementary Table. 1.
Under- and over-regulated DEGs in green and red leaves during senescence were analysed by gene ontology. The distribution of GO terms in DEGs was very similar for green and red leaves throughout ontogenesis, with red leaves showing higher GO counts for all GO terms (Supplementary Fig. 1). For OE genes of the two morphs, the most abundant GO terms in cellular components were “Membrane” (GO:0016020), “Cell” (GO:0005623) and “Intracellular” (GO:0005622). Concerning molecular function, we detected many genes involved into “Catalytic activity” (GO:0003824), “Transferase activity” (GO:0016740) and “Protein binding” (GO:0005515). Finally, considering biological processes, we retrieved mostly “ Metabolic process” (GO:0008152); “Cellular process” (GO:0009987) and “Localization” (GO:0051179) terms (Supplementary Fig. 1).
Concerning UE DEGs in green and red leaves, the GO distribution of cellular components concerned mainly “Membrane” (GO:0016020), “Cell” (GO:0005623) and “Organelle” (GO:0043226) terms. For molecular function, the most abundant GO terms were “Catalytic activity” (GO:0003824) and “Protein binding” (GO:0005515). Regarding biological processes, GO terms related to “Metabolic process” (GO:0008152) and “Cellular process” (GO:0009987) were mainly found (Supplementary Fig. 1).
GO enrichment analysis for specific DEGs of red and green leaves throughout senescence
GO enrichment analyses were performed on DEGs activated or repressed specifically in green and red leaves during ontogenesis. For over-expressed DEGs, Fisher tests were run on 406 and 1,235 activated genes from green and red leaves, respectively (Fig. 1). Fifteen terms were significantly GO-enriched in OE transcripts of green leaves, involved, for example, in “Transferase activity” (GO: 0016740), “Catalytic activity” (GO:0003824), “Oxidoreductase activity” (GO: 0016491) and “Metabolic process” (GO:0008152) (Fig. 2). In OE genes of red morphs, we found 95 enriched GO terms, involved, for example, in “Response to stress” (GO: 0006950), “Response to stimulus” (GO: 0050896) and “Carbohydrate binding” (GO: GO:0030246) (Fig. 3). Similarly, GO-enrichment was performed for 399 and 1,426 under-regulated genes of green and red leaves throughout senescence, respectively (Fig. 1). Only 5 enriched GO terms were found in under-regulated DEGs of green leaves, and all were involved into photosynthesis regulation (Fig. 2). Thirty-six enriched GO terms were found for under regulated DEGs of red morphs, they were involved, for example, in “Carbohydrate metabolic process” (GO: 0005975), “Chorismate mutase” (GO:0004106) and “Serine-type carboxypeptidase activity” (GO:0004185) (Fig. 3).
MapMan and KEGG analysis on DEGs of red and green morphs during senescence
MapMan was run on all DEGs of both morphs in order to compare functional classes during senescence. More genes related to the secondary metabolism were regulated in red compared to green leaves (Fig. 4). Considering phenylpropanoids, overall 37 DEGs were retrieved in red leaves (17 UE, 20 OE) compared to 22 in green leaves (8 UE, 14 OE) (Fig. 4). Amongst these, we detected three “Cinnamyl alcohol dehydrogenase” encoding transcripts that were specifically activated in red leaves and a “Hydroxycinnamoyl-CoA shikimate” protein, which was expressed only during the ontogenesis of green leaves.
Regarding genes involved in flavonoid and anthocyanin biosynthesis throughout senescence in the red morph, we retrieved 13 and 12 OE and UE transcripts, respectively (Fig. 4). For example, a HXXXD-type acyl-transferase encoding transcript, homologous to the Prunus mume UDP glucose-flavonoid-3-O-glucosyltransferase (UFGT) was up-regulated. For the green morph, 16 DEGs were included in these functional classes and most of them were down-regulated such as a transcript encoding another member of the HXXXD-type acyl-transferase family (Fig. 4 and Supplementary Table. 1).
Analysis on the “Photosynthesis” map, focusing on genes involved in light reactions, showed that a major under-regulation of this functional class occurred during ontogenesis in both genotypes, and especially in green leaves (Fig. 4). Differences between genotypes were mostly found in the regulation of genes involved in photosystem I, such as those encoding “Subunits G, I, and H-1” and over-expression of a “PsbQ-like 1” transcript.
Finally, exploring the “biotic stress” bin in MapMan and considering functional classes such as “Glutathione-S-transferase”, “Secondary metabolites involved in stress”, “Beta-glucanase” and “Pathogen-Related proteins“ (PR), we retrieved a major regulation of the red morph throughout senescence with overall 230 vs. 129 DEGs, respectively (Fig. 4). Functional classes, such as glutathione-S-transferase, PR-proteins and beta-glucanases, showed substantial differences in gene regulation between genotypes, as shown in Fig. 4.
KEGG analysis was performed in order to analyse carbohydrate pathways for over-regulated DEGs during senescence. Concerning the “Starch and sucrose metabolism”, we detected 13 activated pathways, among these 9 were shared between the two morphs (Fig. 5). A gene encoding the enzyme that converts glucose 1-P to glucose-6-P was specifically activated in green leaves, whereas enzymes implied into trehalose and ADP-glucose synthesis were exclusively activated in our red morph. These enzymes encode for a “Phosphoglucomutase” in green leaves and three “Trehalose 6-phosphate synthases” and a “Nudix hydrolase isoform 14” in the red morph. No differences were detected for this pathway for under-regulated DEGs of green and red leaves during ontogenesis.
Discussion
The process of senescence is the final stage of leaf development, involving a massive degradation of cellular components with nutrient resorption and ROS accumulation on senescent leaves through highly regulated molecular processes1,6,15,16.
P. cerasifera green and red morphs showed physiological differences during senescence in terms of photosynthetic rates, carbon metabolism and photoprotective mechanisms10. In addition, it was suggested that, besides a photoabatement role, anthocyanins might also be synthesised to avoid a sugar accumulation that could trigger leaf senescence phenomena, thus extending the leaf lifespan in the red morph10.
In this study, for the first time, a transcriptome analysis of P. cerasifera leaves was performed to analyse differences in gene expression and cell functions between red and green leaves genotypes during the process of senescence. In addition, RNA-Seq analyses were conducted on the same saplings used by Lo Piccolo et al.10; hence, molecular regulation of transcripts underpins relevant physiological and biochemical data already observed, completing the biological investigation of leaf senescence process in two different genotypes of P. cerasifera.
Transcriptomic approach evidenced a general higher gene regulation of red leaves compared to green ones during ontogenesis; nevertheless, many DEGs were shared between the two morphs as shown in Fig. 1 and Fig. 4. The occurrence of commonly regulated genes between genotypes can be explained considering that both leaf morphs cope with the same process of senescence. In fact, many of the common genes are related to leaf aging. Among these genes, we retrieved transcription factors (of the NAC, MYB, AP2, YIPPEE and WRKY families) which are known to regulate senescence in leaves, SAG genes, whose expression increases during leaf aging controlling developmental process, cell wall loosening/remodelling-related transcripts (expansins, pectin-methyltransferases, exostosin) and genes related to nutrient remobilisation, such as vacuolar sorting receptor proteins, xanthine/uracile permeases and purple acid permease (see Supplementary Table. 1)2,5,9,30.
Although many transcripts were shared between red and green morphs during leaf senescence, while some others showed remarkable differences. Red leaves showed more DEGs involved in phenylpropanoid synthesis throughout. Some transcripts related to this pathway are involved in leaf stress prevention (Fig. 4), such as those encoding cinnamyl alcohol dehydrogenase (CAD), which produces monolignols involved in plant defence and activated during senescence in Arabidopsis41,42. Concerning cell stress response genes differentially expressed during ontogenesis, we detected considerable differences between green and red leaves, with an extensive over-expression of glutathione-S-transferase genes (GST) and Pathogen-Related (PR) genes in green leaves and an extensive down-regulation of beta-glucanase and serine-type carboxipeptidases transcripts in our red morph (Figs. 3 and 4). The GST and PR families are induced by several stresses and are known to be upregulated during leaf senescence in several species9,43,44. These enzymes play pivotal roles in both cell detoxification and defence against pathogens44,45. On the contrary, beta-glucanases and serine-type carboxypeptidases are mainly involved in cell wall loosening during leaf senescence46,47,48. These data may suggest that the red morph copes with leaf aging by activating the expression of genes involved in stress response and ensures cell wall integrity, maintaining more “healthy” leaves compared to the green one.
Surprisingly, genes involved in the shikimate pathway (e.g. encoding chorismate mutase and hydroxycinnamoyl-CoA shikimate transferase), which are related to defence mechanism and aromatic amino acid (AAA) production49,50,51, seem to be activated in green leaves, but down-regulated in the red morph. Recently, it has been established that shikimate metabolism is far more active in red leaves of basil compared to green ones27. Nevertheless, the gene expression of this pathway during ontogenesis in different morphs is little known.
In our experiments, we also retrieved more regulated specific genes of the flavonoid pathway in red morph leaf ontogenesis compared to the green morph (Fig. 4), suggesting a possible transcriptional regulation of anthocyanins synthesis occurring in red leaves throughout the senescence process, as observed at the physiological and biochemical levels by Lo Piccolo et al.10. In this sense, a transcript encoding a homolog to UDP glucose-flavonoid-3-O-glucosyltransferase (UFGT) was specifically up-regulated only in the red morph (Fig. 4, Supplementary Table. 1). This protein plays a key role in anthocyanin stability and solubility, transferring a glucosyl group from UDP-glucose to the 3-hydroxyl anthocyanidins52. In addition, many beta-glucosidase transcripts encoding enzymes involved in anthocyanins turn-over and stability53 were differentially expressed in both genotypes; however, two transcripts related to these enzymes were specifically over-expressed with a high fold change in red leaves (Supplementary Table. 1). Worthy of notice is the differential expression of three threalose-6-phosphate synthases, detected exclusively in the red morph (Fig. 5). These enzymes play a key role in threalose-6-phosphate production, an essential signal metabolite that is essential together with sucrose to stimulate anthocyanin synthesis54. In fact, anthocyanins might further act as sugar sinks7,8, thereby helping to avoid a carbohydrate accumulation in leaves and the consequent sugar-induced senescence10. Moreover, other genes involved in sugar metabolism were retrieved during the experiment, e.g. GDSL-like lipases that are involved in lipid breakdown. Their expression has been shown in senescing leaves of Arabidopsis55. We showed a large down-regulation (with high fold change) of these genes in red leaves, with a consequent possible reduction of carbohydrate mobilisation. The earliest and most dramatic sign of senescence in leaves is the degradation of chloroplasts with consequent breakdown of chlorophyll and the down-regulation of genes related to photosynthesis1,2,9,56. We retrieved an overall major down-regulation of photosynthetic functions of green leaves in P. cerasifera compared to red ones, as shown both by the GO terms and by MapMan analysis (Figs. 2 and 4). In our experiment, green leaves during early senescence showed a more extensive decrease of gene expression related to photosystem I (PSI) than red leaves, especially for subunits G, I and H, and partially to photosystem II (PSII). A faster degradation of PSI compared to PSII during leaf senescence has been established in several plants such as in rice and beans57,58 (Fig. 4). In addition, a gene encoding for Pheophorbide A Oxygenase (PAO), a key enzyme for chlorophyll breakdown, was detected as being over-expressed only in green leaves but not in the red morph (See Supplementary Table. 1). This gene is highly activated during cell senescence and is involved into chlorophyll breakdown, and mutants for this enzyme have shown an enhanced resistance to aging2,59,60. On the contrary, the red morph showed fewer under-regulated genes involved in photosynthesis (Fig. 4). Especially in ‘Pissardii’, we detected an over-expression of PsbQ-like 1 transcript, encoding for a protein involved in the function of chloroplast NAD(P)H Dehydrogenase complex in Arabidopsis; mutants for these genes lead to a reduction of photosynthetic rate61. These data are in line with our previous physiological work, in which 13-week-old leaves of the red morph showed about a 26% increase in net photosynthetic rate compared to those of green morph10 and with that found in other species21,26,62, suggesting a strong delay in the senescing process in red leaves. Interestingly, transcripts involved in carotenoid biosynthesis, like those encoding lycopene cyclase, were also strongly up-regulated, especially in green leaves (Supplementary Table. 1), suggesting a possible necessity of carotenoid synthesis during the senescence of green leaves of Prunus, as reported by Lo Piccolo et al.10.
In conclusion, P. cerasifera green and red leaves during ontogenesis showed substantial differences. Although many transcripts were shared, the transcriptome of the red morph was highly regulated with differences, compared to the green one, in cellular functions, such as photosynthesis, the anthocyanin pathway, cell stress prevention and nutrient metabolism. Our results suggest that red morphs could regulate a set of genes that can cope with maturity-to-senescence transition leading to a delay of senescence processes.
This dataset offers clear evidence of inherent molecular differences between red and green leaves of Prunus during leaf senescence. In particular, the onset of senescence occurring in red leaves resulted in the activation of anthocyanin-related transcripts and some genes connected to sugar metabolism, which both (sunscreen and sugar-buffering effect of anthocyanins) could explain the higher photoprotection found in these leaves and their delayed senescence when compared to the green counterparts. Indeed, green leaves had higher levels of transcript related to carotenoid biosynthesis, but altogether a more extensive decrease of gene expression related to PSI and PSII degradation and chlorophyll breakdown, which are supportive for an early senescence of the leaf. Anthocyanins accumulate in the range of µM concentration in Prunus leaves during leaf senescence (see the anthocyanin index reported by Lo Piccolo et al.10) and at those concentrations, anthocyanins can abate a considerable amount of light (25–35% of incident light)7. Consequently, anthocyanins efficiently photoprotect the red leaves during foliar senescence.
The present study demonstrates the benefits of being red during the vulnerable phase of leaf senescence and represents the first attempt to summarise molecular changes occurring in foliar senescence in green and red genotypes, thus providing basic information for future research aimed at assessing the eco-physiological roles of anthocyanins and establishing the molecular origin of this intriguing class of flavonoids.
Materials and Methods
Experimental protocol and samplings
Saplings are the same reported by Lo Piccolo et al.10; in particular three-year-old P. cerasifera saplings (clone ‘29C’ with green leaves; var. ‘Pissardii’, with red leaves) were purchased from an Italian nursery (Vivai Battistini, Cesena, Italy). Stems of both morphs were grafted onto ‘29C’ rootstock in November 2016. One month after grafting, saplings were transplanted to 6.5-L pots in a growing medium containing a mixture of Einhetserde Topfsubstrat ED 63 standard soil (peat and clay, 34% organic C, 0.2% organic N and pH 5.8–6.0) and sand (3.5:1 in volume). Saplings of both morphs were maintained under greenhouse conditions until March 2017, when they were transferred to the field. One week after leaf emergence (early July 2017), homogeneous leaves from saplings of both morphs were marked to be followed throughout ontogenesis (sampled at 1, 7 and 13 weeks after leaf emergence). Leaf samples at 7 and 13 weeks (mature and senescent leaves, respectively) were used for molecular analyses10.
RNA isolation and sequencing
Red (R) and green (G) leaves 7 and 13 weeks after emergence (hereafter called mature, M, and early senescent, S, respectively) were collected. Overall, three biological replicates were chosen for each morph, and time point and RNA isolation was performed using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following manufacturer’s instructions, but adding 2% PVP (Wt 40000, Sigma-Aldrich, St. Louis, MO, USA) to Lysis Solution. After elution, DNAse I (Roche) digestion was performed in order to remove genomic DNA traces. Hence, RNA was purified by phenol/chloroform extraction and ethanol precipitation following standard procedures63. Twelve RNA-seq libraries were obtained using the TruSeq RNAseq Sample Prep Kit, according to manufacturer’s protocol (Illumina, San Diego, CA, USA). The poly-A tail was used to isolate the mRNA fraction from total RNA. Then, mRNA was chemically fragmented and subsequently converted to cDNA. Adapters were ligated to the cDNA, and 200 ± 25 bp fragments were gel purified and enriched by PCR. Libraries were analysed by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and sequenced with Illumina HiSeq. 2000 (Illumina) using version 3 reagents. Single-end read sequences of 75 bp length were collected. Overall quality was checked by FastQC (v. 0.11.5) and improved by Trimmomatic64 (v. 0.36) using the following parameters: SLIDINGWINDOW: 4:20; HEADCROP: 15; MINLEN: 60. Any ribosomal traces were removed from libraries using CLC Genomic Workbench (v. 9.5.3, CLC-BIO, Aarhus, Denmark) aligning reads on Prunus rRNA obtained from the SILVA repository65.
Differential expression and gene ontology analysis
High quality trimmed reads were aligned on the reference transcriptome of P. persica (version 2.1, https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias = Org_Ppersica) using CLC Genomic Workbench as follows: mismatch cost = 2; insertion/deletion cost = 3, length fraction = 0.8, similarity fraction = 0.8. CLC Genomic Workbench distributes multi-reads among similar sequences up to ten sequences; this strategy allows a correct estimation of closely paralogous genes66.
The R package “edgeR” was used to analyse raw counts per transcripts (Robinson et al. 2010); gene expression was calculated as reads per kilobase per million reads mapped66 (RPKM), and only genes with RPKM > 1 in at least one library were retained. Statistical tests by “edgeR”67 were performed between counts of mapped reads for each gene in mature and senescent leaves samples of both genotypes (MG vs. SG; MR vs. SR) using the quasi-likelihood test as described by the user’s guide instructions and as suggested by Anders et al.68. Resulting P-values were corrected with the False Discovery Rate69 (FDR) and genes with FDR < 0.05 were selected as significant.
A gene was considered differentially expressed when the RPKM value in the senescent leaves was at least two fold higher or lower than in mature leaves. In this way, we were able to distinguish differentially expressed genes in two groups: up- and down-regulated.
Annotations and GO terms for each gene were downloaded from the phytozome database of P. persica. GO enrichment was performed using Blast2GO70 with the Fisher exact test comparing GO term distribution in the DEGs vs. the whole transcriptome GO id annotation of P. persica. Subsequently P-values were filtered by FDR (corrected P-value < 0.05). REVIGO71 was performed to reduce GO complexity using the parameter “tiny similarity”. In addition, “GO slim” was run by Blast2GO70 in order to identify major ontology classes.
MapMan was used for the functional analysis of DEGs; bins of P. persica were supplied with the software72. KO id codes for DEGs were obtained using KAAS73, and corresponding codes were submitted to KEGG74 for pathway network analysis (Kyoto Encyclopaedia of Genes and Genomes); KAAS alignment was performed by default parameters except for “single directional best-hit” (SBH), similarly to the work described by Torre et al.27.
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Acknowledgements
This study was performed in the framework of PRA 2017 project Photo-oxidative stress in juvenile and senescent red vs. green leaves financed by the University of Pisa.
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L.G., A.C., L.N., R.M., C.N., P.V., T.G. conceived and designed research. A.V., E.L.P., M.L., G.L., F.M., E.P., G.R., D.R. conducted experiments. A.V., E.L.P., T.G., L.G. analysed data. A.V., A.C., T.G., L.G. wrote the manuscript. All authors read and approved the manuscript.
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Vangelisti, A., Guidi, L., Cavallini, A. et al. Red versus green leaves: transcriptomic comparison of foliar senescence between two Prunus cerasifera genotypes. Sci Rep 10, 1959 (2020). https://doi.org/10.1038/s41598-020-58878-8
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DOI: https://doi.org/10.1038/s41598-020-58878-8
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