# Integrated Green Chemical Approach to the Medicinal Plant Carpobrotus edulis Processing

## Abstract

Many plants have medicinal properties due to substances known as phytochemicals. To utilize these plants in practice, numerous procedures, such as extraction, isolation and characterization methods and toxicology and bioactivity studies, must be designed and implemented. Integrated approach to process Carpobrotus edulis, a weed medicinal plant widely spread in Portugal, was developed into a closed loop of two processes: microwave assisted extraction (MAE) and activation (MAA), to produce both phytochemicals and biochar. The use of MAE for phytochemical extraction was shown to be more energy efficient than conventional Soxhlet extraction: the process time was decreased by 7–8 times, and the energy efficiency was increased by up to 97%. The yield of the extracts is of 27%. Qualitative and quantitative identification/characterization of the phytochemicals were performed by LC-MS and phytochemical screening assays. The results clearly indicated that Carpobrotus edulis is rich by flavonoids (up to 24%). The use of MAA to process the residual biomass could shorten the activation time, resulting in reduced energy consumption. Biochar with a high yield of 65% (on a biomass basis) and a well-developed texture (surface area of 68.9 m2/g; total pore volume of 0.10 cm3/g; micropore volume of 0.07 cm3/g) is obtained.

## Introduction

For thousands of years, humans have used plant sources to fight illnesses. Initially, plants were used to brew herbal teas and to make a variety of infusions. To date, a number of different techniques have been developed for the efficient purification, separation and characterization of individual compounds and their mixtures, which have a variety of useful biologically active properties, such as antiviral, antioxidant, and antibacterial properties. The European phytochemical and plant extract market is estimated to grow from $833.7 million in 2014 to$1.25 billion by 2019, driven by the increasing health awareness of consumers in the region1.

According to the World Health Organization (WHO), nearly 20,000 medicinal plants exist in 91 countries2. Furthermore, all countries have at least one medicinal plant that grows like a weed and does not require special care.

The wild-growing plant Carpobrotus edulis (C. edulis) is a fast-growing weed with pronounced medicinal potential3 that flourishes along coastal areas in many parts of the world4, such as the Mediterranean, South Africa, North and South America, Australia, etc. C. edulis leaf juice, a non-destructive use of the plant, is well known for its wide range of antifungal and antibacterial external applications5, such as the treatment of diarrhoea, eczema, tuberculosis, throat and mouth infections; soothing itching caused by spider and tick bites; and the treatment of wounds and burns6,7,8. These effects are due to the presence of bioactive compounds—phytochemicals—in the juice. However, the phytochemical composition varies depending on the location of the natural source of the plant, causing differences in bioactivity among samples of the same species of plant from different regions9,10,11.

Quantitative phytochemical analysis of C. edulis extracts has revealed a high percentage of phytochemicals from the phenolic family (up to 50–60%); this family is well known and widely used because of its strong antioxidant properties and high biological activities12.

To utilize such biologically active compounds in practice, several procedures must be conducted, namely, extraction, isolation, characterization and/or phytochemical screening, toxicological evaluation, extended testing of in vitro and in vivo bioactivity, etc.13.

Another problem is that none of the currently known and used plant processing techniques meet all the requirements of economics, safety and scalability. The primary challenges in the economically viable commercial production of phytochemicals lie in the high feedstock cost, the high extraction cost of valuable bioactive compounds, their relatively low yield, and the consequently high content of residual waste biomass14,15.

The use of abundant weed plants (called low-value feedstocks) could help to overcome some of the mentioned limitations. However, while the technical feasibility of phytochemical extraction from weed plants has been demonstrated16,17, there have still been few advances in the economic feasibility of phytochemical production from weed plants due to the low yield of the target valuable products (1–15%) and the large amount of residual waste biomass (up to 99%) to be disposed17.

Therefore, in the present study, a widely distributed plant along the coastal zone of Portugal, C. edulis, was chosen as a renewable low-cost feedstock for advanced processing development. First, there is a need for additional research into the extraction of valuable phytochemicals from Portuguese sources of this weed plant. Second, based on our preliminary techno-economic estimations, it is also expected that the introduction of additional recovery pathways for the biomass waste (solid residue) obtained after extraction will help meet the requirements of economic feasibility for phytochemical extraction from weed plants18,19,20. The development of a biomass utilization pathway accompanying the extraction process will lead to an almost “zero-waste” full-chain network of valuable products (both primary (phytochemicals) and secondary (biochar)), which could be applicable to a variety of low-value feedstocks and thus aid in the creation of economically feasible weed plant processing schemes for practical usage in the future21,22.

Therefore, the main aim of the present work is to develop a feasible lab-scale scheme for medicinal weed plant processing. To achieve this aim, the proposed advanced C. edulis processing scheme is designed to be an integrated closed loop consisting of two green processes: (i) microwave-assisted extraction (MAE) to produce phytochemicals (with an emphasis on the flavonoid sub-family) and (ii) microwave-assisted activation (MAA) to produce bio-fertilizer from the residual biomass (waste after extraction).

The overall work is also designed to comply with green chemistry principles23, namely, with the following five: preventing waste, safer chemicals and products, safer solvents and reaction conditions, increasing energy efficiency and renewable stocks. Furthermore, the work is based on a chemical engineering approach, in which chemical processes are designed to convert raw materials into valuable products for further practical usage.

## Results and Discussion

### Quantitative characterization of the extracts

A number of solvents/solvent mixtures, such as H2O, MeOH, EtOH and EtOH/H2O with different ratio, were studied for the extraction of the phytochemicals from C. edulis using MAE. The conditions of the extraction were optimized to maximize the yield of the phenolic compound family, with an emphasis on the flavonoid sub-family; this yield was determined quantitatively using phytochemical screening assays, as described in experimental part 2.3.2. Data are presented in Table 1.

The EtOH/H2O extract exhibited the highest yield of targeted phenolics (up to 21–22%) and flavonoids (16–18%). While, phytochemicals yields were similar in a case of different ratio (30%, 50% and 70%) of the EtOH/H2O solvent. Therefore, to address the green chemistry principle on safer solvents and auxiliaries for further optimization of MAE conditions, the solvent with maximum water content, i.e. EtOH (30%)/H2O (70%) was chosen.

The optimization conditions for MAE was carried out in a stepwise manner for ratio of raw material/solvent (1/5, 1/10, or 1/15 (m/v)); time (15, 20, 25, 30, 35, 40, 45 or 50 min); temperature (70, 80, 90, or 100 °C); and number of repeats of the extraction process (1, 2, 3, 4, 5, or 6 times).

Based on the data from the phytochemical screening assays, the following optimal conditions for the MAE process were determined: solvent - mixture of EtOH (30%) and H2O (70%); ratio of raw material/solvent -1/15 (m/v); extraction time −40 min; and temperature −70 °C.

Quantitative phytochemical analysis using phytochemical screening assays was also performed for the extracts obtained through Soxhlet extraction. The conditions for Soxhlet extraction were adjusted based on the optimal conditions obtained for MAE, namely, the optimal solvent [mixture of EtOH (30%)/H2O (70%)] and optimal ratio of material/solvent [1/15 (m/v)].

Using the same optimization procedure (i.e., based on the yield of the target phytochemicals), the following optimal conditions for Soxhlet extraction were determined (Table 2): extraction time − 260 min; and temperature −86 °C.

The optimal conditions for MAE were compared with optimal conditions for Soxhlet extraction in terms of energy efficiency (Table 3) [energy consumption required to produce the same amount of the target phytochemicals using the same optimal solvent time and initial ratio of materials]. The energy consumption was calculated according to Eq. (1), taking into account the power rating of the device (either microwave oven or Soxhlet) (in kW) multiplied by the operation time (in hours) required to produce the target phytochemicals.

Comparison of the results confirmed that the energy consumption required to deliver the target phytochemicals at the desired yield by Soxhlet extraction was 32 times higher than that for the MAE process. This difference means that the energy efficiency of MAE is 97% greater than that of the Soxhlet extraction process.

### Qualitative characterization of the extracts

The results of LC-MS analysis of the EtOH (30%)/H2O (70%) extract obtained under optimal conditions of MAE are presented in Fig. 1.

#### Composition of phenolic compounds

The C. edulis extract fingerprint was obtained by HPLC-DAD. The chromatographic profile indicated the separation of 30 main compounds that, based on the observed UV absorption maxima, fall into two subclasses of phenolic compounds. The main group of peaks appearing between 12 and 25 min exhibit absorbance maxima at approximately 278–280 nm, suggesting the presence of flavan-3-ols and proanthocyanidins (PAs), whereas the eleven peaks eluting between 26 and 37 min present UV-Vis absorption maxima at approximately 268 and 350 nm, revealing flavonol structures containing an O-glycan part in their skeleton. Figure 1 shows the base peak chromatogram obtained by LC-ESI(-)/QTOFMS analysis of C. edulis extract prepared by MAE. The 30 compounds were identified on the basis of their accurate m/z values as deprotonated molecules [M-H] and MS/MS spectra. Polyphenol identification and peak assignment were also based on comparison with similar published data. Table 4 presents the retention times, molecular formulas, exact and accurate [M-H] (m/z) values, and main MS2 product ions for each compound.

Peaks 1, 2 and 3 (Rt = 2.4, 5.5 and 8.0 min) with deprotonated molecules at m/z 191.0197, 315. 0724 and 329.0867 were attributed to phenolic acid derivatives based on their accurate MS and MS2 data.

Peaks 8 and 11 (Rt = 13.2 and 15.8 min) displayed an ion with m/z 465.1047 in the negative ESI mass spectra, which produced product ions in the MS2 spectra with m/z 303.0482 due to the loss of 162 u, indicating the presence of a glucose residue linked to a taxifolin aglycone. Compounds 8 and 11 were fully identified based on their accurate mass measurements as taxifolin-O-glucoside isomers.

The thirteen peaks (4, 5, 6, 7, 9, 10, 12, 13, 14, 15, 16, 17, 18 and 19) observed in the ESI (-) base peak chromatogram (Fig. 1) were identified as flavan-3-ols and PAs, a class of oligomers of catechins and their enantiomers. The compounds were tentatively assigned based on accurate mass measurement data and by comparison with published data24,25,26,27.

Peaks 4, 5, and 6 (Rt = 9.1, 9.4 and 11.6) were attributed to catechin-O-glycoside derivatives based on the MS2 spectra, which displayed an ion with m/z 289.0723 attributed to deprotonated catechin, resulting from the loss of one or two hexose residues. Compounds 4 and 6 were identified as catechin-O-glucoside isomers, whereas compound 5 was attributed to a catechin-O-diglucoside.

Peaks 7 and 12 (Rt = 12.6 and 16.6 min) produced deprotonated molecules with m/z 577.1358 in the MS spectra, which yielded peaks at m/z 425.0886, 407.0772 and 289.0722 as the most abundant fragment ions in the MS2 spectra. The loss of 152 u via a retro Diels-Alder reaction indicates a fragmentation pattern characteristic of proanthocyanidin dimers of the type (epi)catechin-(epi)catechin. Compounds 7 and 12 were assigned as B-type procyanidins.

Peaks 9 and 13 at Rt 13.6 and 17.5 min, respectively, which had deprotonated molecules with accurate m/z 289.0745 and 289.0718, were assigned to (+)-catechin and (−)-epicatechin.

Peaks 10 and 16 (Rt = 15.1 and 20.2 min) were attributed to B-type procyanidin trimers based on their deprotonated molecules at m/z 865.2011 and 865.1998, respectively, and their main fragment ions at m/z 577.1352, 289.0719 and 287.0564.

Peaks 14 and 18 (Rt = 19.0 and 23.4 min), yielding doubly charged species [M-H]2− in their MS spectra with accurate m/z 720.1585 and 720.1591, respectively, were identified as b-type procyanidin pentamers. The main fragmentation pathway in the MS2 spectra corresponds to a pair of two monocharged species: one at m/z 289.0720 (base peak) assigned to a deprotonated (epi)catechin, and the other at m/z 1152.2520, which corresponds to a deprotonated molecule of a b-type procyanidin tetramer.

Peaks 15 and 19 (Rt = 19.3 and 24.8 min) were also attributed to doubly charged species based on the isotopic distribution observed for the deprotonated molecules [M-H]2− with m/z 864.1918. Compounds 15 and 19 were identified by accurate mass measurements as b-type procyanidin hexamers.

Peak 17 at Rt = 22.0 min presented a signal at m/z 1153.2631 in the MS spectrum, which yielded main product ions with m/z 983.2058, 865.1919, 577.1204 and 287.0564 in the MS2 spectrum, suggesting a species composed of 4 (epi)catechin units. Based on the accurate data, compound 17 was identified as a b-type procyanidin tetramer.

Peaks 2030 shown in Fig. 1 at higher retention times were attributed to O-methylated flavonol derivatives based on their accurate MS and MS2 fragmentation behaviour under ESI negative ion mode analysis.

Peaks 20, 21, and 22 (Rt = 26.4, 26.8 and 27.2 min) exhibited deprotonated molecules with m/z 769.2201, 639.1565 and 799.2306, which gave peaks at m/z 315.0574, 331.0448 and 345.0606 in the MS2 spectra, respectively, attributed to the aglycone ions (Y0). The first and last peaks result from the loss of 454 u (146 u + 308 u), indicating that the glycoside part contains two deoxyhexoses (146 u) and one hexose (162 u) units. Intense signals at m/z 314.0439, 330.0386 and 344.0541 were also observed, assigned to (Y0H)−•; the presence of these radical ions indicates O-di-glycoside derivatives28. The MS2 spectrum also displayed signals at m/z 299.0208, 315.0150 and 329.0603, arising from the fragmentation of the aglycone part. The loss of 16 u suggests an O-methylated flavonol with methoxyl groups attached to ring B. Based on literature data29, compounds 20, 21 and 22 were assigned to isorhamnetin-3-O-rutinoside-rhamnoside, laricitrin-3-O-rutinose and syringetin-3-O-rutinoside-rhamnoside, respectively.

Peak 23 (Rt = 28.1 min) showed a deprotonated molecule at m/z 755.2041 and MS2 fragments at m/z 315.0500 and 314.0437, corresponding to the isorhamnetin aglycone ion (Y0) and its radical ion (Y0H)−•, respectively. A peak at m/z 623.1619 (−132 u) confirmed the presence of a pentose residue on the sugar moiety. Based on the accurate mass measurements, compound 23 was attributed to isorhamnetin-3-O-rutinose-pentoside.

As shown in Table 4, two compounds coeluted in peak 24 at a retention time of 29.1 min. The MS spectrum displayed two peaks at m/z 785.2162 and 623.1625, which generated product ions at m/z 345.0615 and 315.0500 in the MS2 analysis, assigned to syringetin and isorhamnetin aglycones, respectively. The MS2 spectra of both precursor ions also presented characteristic abundant radical ions (Y0H)−• (m/z 344.0548 and 314.0436, respectively), confirming the presence of O-di-glycosides. Peak 24 was also attributed to two O-methylated flavonols, syringetin-3-O-rutinoside-pentoside and isorhamnetin-3-O-rutinoside.

The base peak chromatogram also showed peaks 25, 26, 27 and 28 (Rt = 29.6, 31.2, 32.2 and 33.6 min), yielding deprotonated molecules at m/z 653.1721, 623.1618, 653.1717 and 507.1146, respectively. Precursor ions at m/z 653.1721 and 507.1146 fragmented into syringetin aglycone at m/z 345.0609, whereas the precursor ion at m/z 623.1618 lost 308 u, leading to m/z 315.0504, which corresponds to isorhamnetin aglycone. Based on these accurate data, compounds 25, 27 and 28 were assigned to syringetin-O-glycoside derivatives: compounds 25 and 27 were attributed to syringetin-O-rutinoside isomers, 26 was assigned to an isorhamnetin-O-rutinoside isomer, and 28 was assigned to syringetin-O-glucoside.

Peak 29 at Rt = 35.1 min exhibited a deprotonated molecule at m/z 435.1296 in the MS spectrum, which fragmented into two abundant ions at m/z 276.0396 (loss of 162 u) and 167.0347. Based on the accurate mass measurements, compound 29 was identified as a dihydrochalcone derivative, phloretin-glucoside.

Peak 30 at Rt = 35.8 min had a deprotonated molecule at m/z 961.2617, which presented the characteristic ion of syringetin aglycone at m/z 345.0611 in the MS2 spectrum. The MS2 spectrum also showed two peaks at m/z 799.2117 and 767.2038, corresponding to the loss of 162 and 176 u, respectively, indicating the presence of hexoses and glucuronyl moieties in its skeleton30. Compound 30 was identified as syringetin-rutinoside-glucuronyl-pentoside based on reported data.

The obtained results for the C. edulis extracts clearly indicated that this natural widely distributed weed and medicinal plant is rich in B-type procyanidin oligomers, dihydroquercetin derivatives and O-methylated flavonol derivatives. From thirty separated peaks, three correspond to the three pure compounds from phenolic acids family and other twenty-seven peaks are related to seven different flavonoids compounds known as chemopreventive agents in cancer therapy and strong inhibitors of free radical formation31.

### Biomass MAA. biochar properties

The main objective of this part of work was elaboration of the Microwave Assisted Activation (MAA) process to proceed with the residual biomass to address the resulted solid product -biochar.

The effect of the microwave operational parameters, including temperature (200–350 °C) and irradiation time (5–30 min), at a constant power of 600 W on the biochar yield and its textural characteristics, such as the BET surface area, total pore volume, pore size distribution, and the point of zero charge (PZC value), were investigated to optimize MAA conditions.

#### Effect of temperature

Figure 2 presents the effect of temperature on biochar yield (open squares) and specific surface area (filled triangles) after MAA biomass processing. In the temperature range deployed (200–350 °C), the yield of biochar was maximum at 250–300 °C and then decreased with further increases in temperature. The surface area of the resulting biochar product continuously increased with temperature and ranged from 54 to 72 m2/g under the chosen experimental conditions. At high temperatures of ≥350 °C, over gasification most likely occurs, resulting in reduced surface area/porosity with progressively decreasing carbon yield32.

Therefore, it can be concluded that MAA can be applied as a thermal biomass processing technique to produce a biochar product with high quality and high yield (ca. 60-65%) at a relatively low temperature (approximately 250 - 300 °C) in a short activation time (10 min), while in the case of conventional thermal biomass processing (pyrolysis or/and gasification), heating at a higher temperature and longer activation time is required to obtain similar results32.

#### Effect of microwave irradiation time

Microwave irradiation time is another factor affecting the biochar yield and properties. The effect of irradiation time in the range of 5–30 min on the yield and surface characteristics of the bio-fertilizer was investigated at a constant microwave input power of 600 W and a temperature of 250 °C (Fig. 3).

Generally, both measured parameters (biochar yield and surface area) increased with time, exhibiting maximum values at an irradiation time of 10 min. Beyond this limit, the surface area parameters slightly decreased (from 72 to 58 m2/g), while a sharp decrease in biochar yield from 65 to 26% was observed as the irradiation time was prolonged from 10 to 30 min. The experimental results confirmed that microwave heating could shorten the activation time and, remarkably, produce a high-quality biochar.

The results implied that the optimal irradiation time (ca. 10–15 min) at the optimal temperature of 250 °C promoted thermal energy, which was applied in the activation process and enhanced the surface area (up to ca. 70 mg2/g) and internal porosity (up to 0.10 cm3/g) of the resulting biochar while producing a high product yield of 60–65%.

Therefore, the optimal conditions of the MAA process were evaluated to process waste biomass after phytochemical extraction from C. edulis. The conditions are as follows: irradiation time of 10–15 min, temperature in a range of 250–300 °C, and constant input microwave power of 600 W. Beyond these conditions, further microwave treatment might produce local overheating, which would considerably increase carbon burn-off, thus destroying the pore/surface texture and yield of the resulting biochar. At low microwave power levels, an irradiation time lower than 5 min and/or a temperature lower than 200 °C, no activation was observed; i.e., microwave irradiation had no effect on the surface area and total porosity of the product.

The detailed textural and surface characteristics of the biochar obtained under optimal MAA conditions are summarized in Table 5. The surface area was 68.9 m2/g; the total pore volume was 0.10 cm3/g, 0.07 cm3/g of which was the micropore volume; and the fixed carbon content was 64.9 wt %, expressed on a dry, ash-free C. edulis feedstock weight basis. The biochar PZC of 6.9 is neutral, reflecting the fact that the low temperature of the MAA process (approximately 250 °C) allowed partial retention of the acidic functional groups on the biochar surface, thus enabling the effective use of the resulting biochar as bio-fertilizer for both acidic and basic types of soil.

The results are compared with biochar properties from the literature in Table 5, obtained from different plant-derived biomass sources using various thermal methods, including MAA. The properties of the bio-fertilizer product obtained by applying the MAA process to waste biomass are considerably different from those of products obtained from both slow and fast pyrolysis of biomass residues. Despite the low pyrolysis temperature, MAA preferentially generated biochar with the highest fixed carbon content (Table 5, MAA processes32 and [present work]), yielding 40–65 wt % biochar. The same tendency is evident for low-temperature slow pyrolysis conditions33, where the fixed carbon content of the resulting biochar is still high (ca. 22%32) in comparison with that obtained under high-temperature fast pyrolysis of biomass34.

The surface area and total porosity of the MAA biochar are higher than those obtained by slow pyrolysis in a similar temperature range above 250 °C, while the values are lower than those obtained with the use of fast high-temperature pyrolysis or microwave activation processes using chemical or physical activating agents to produce activated carbon35.

Several researchers have compared microwave pyrolysis with conventional pyrolysis and identified considerable differences between the thermal methods32,33,34,35,36, which were attributed to the activation process of biomass under microwave irradiation assisted by activation agents or fast and slow pyrolysis. However, to the best of our knowledge, only a few studies have reported microwave biochar production and direct comparisons of the properties of biochar obtained by conventional pyrolysis and MAA.

### Total mass balance

The total mass balance for the C. edulis processing method was estimated on a dry matter (water-free) basis with respect to the leaf mass (i.e., after pre-drying), which was considered to be 100%.

The total mass balance data for the C. edulis processing method are presented in Fig. 4 for the developed integrated process consisting of two green pathways: (i) MAE, to produce phytochemicals (with an emphasis on the flavonoid sub-family) and (ii) MAA, to produce bio-fertilizer from the residual biomass (waste after extraction).

## Conclusions

The work accomplished the design of two processes, the MAE of C. edulis, a natural weed plant, and MAA, to process the biomass waste after extraction, which were integrated into a single process. The result delivers two green products, phytochemicals (with a dry extract yield of 27%) and bio-fertilizer (with a yield of 47% on a dry C. edulis basis or 65% on a biomass weight basis). The use of MAE for phytochemical extraction from C. edulis was shown to be more energy efficient than conventional Soxhlet extraction: the process time was decreased by 7–8 times, and the energy efficiency was increased by up to 97%.

The results of HPLC-DAD-ESI-HRMS analysis of the aqueous extracts clearly indicated that C. edulis is rich in members of the flavonoid sub-family; namely, 7 of the 10 obtained pure compounds are flavonoids with strong antioxidant, anti-inflammatory, antiradical and antibacterial activities.

The experimental results showed that applying the MAA process to biomass (waste after phytochemical extraction from C. edulis) could shorten the activation time, resulting in reduced energy consumption, and produce a high-quality biochar. The optimal conditions for the MAA process were determined: an irradiation time of 10–15 min and temperature range from 250–300 °C at a constant input microwave power of 600 W.

Biochar with a high yield of 65% (on a biomass basis) and a well-developed texture (surface area of 68.9 m2/g; total pore volume of 0.10 cm3/g; micropore volume of 0.07 cm3/g; fixed carbon content of 64.9 wt %; neutral pHPZC of 6.9) was obtained. From this point of view, the obtained bio-fertilizer and its properties are comparable to analogues in the literature for both acidic and basic soil fertilization.

## Methods

### Materials

#### Plant material

C. edulis, a plant that is naturally widespread in Portugal, was chosen as a renewable feedstock for phytochemical production. Fresh leaves of C. edulis plants were collected in the coastal zone of central Portugal (38°36′58.4″N, 9°13′01.8″W). The plants were authenticated by a gardening centre (Centro de Jardinagem da Sobreda) in Setubal, Portugal. The collected material was air-dried at room temperature for 25 days, then homogenized by grinding to a fine powder and stored in airtight bottles.

#### Solvents used for the extraction step

A number of solvents and solvent mixtures were studied for the extraction of phytochemicals from C. edulis. In consideration of the green principle of safer solvents and noting that the application of microwave energy to highly flammable organic solvents may cause hazards, based on the literature, the following solvents were chosen as the most useful for this type of application: H2O, MeOH, EtOH, mixture EtOH (70%)/H2O (30%), EtOH (50%)/H2O (50%) and EtOH (30%)/H2O (70%).

#### Standards and reagents used for phytochemical screening assays

The chemicals and solvents used for the phytochemical screening assays were supplied by Sigma Aldrich (Steinheim, Germany) and were of analytical grade (A.R.) and/or reagent grade (R.G.). The materials included a mixture of phosphomolybdate and phosphotungstate (Folin-Ciocalteu phenol reagent); gallic acid (C6H2(OH)3COOH); quercetin (3,3′,4′,5,6-pentahydroxyflavone); rutin trihydrate (C27H30O16·3H2O, quercetin-3-rutinoside trihydrate); Na2CO3; Al2O3; CH3COONa; NaNO2; AlCl3 and 1 N NaOH.

The materials used for the analysis of phytochemicals in extracts, HPLC-grade methanol (99.9%), acetonitrile (99.9%) and water solution containing 0.1% (v/v) formic acid, were supplied by Sigma-Aldrich (Steinheim, Germany).

### Experimental procedures

#### Microwave-assisted extraction (MAE)

MAE was carried out using a microwave synthesis reactor (Monowave 300, Anton Paar GmbH, Austria). For the extraction, a dry crushed sample was placed in a G30 borosilicate glass vial and placed in an oven. To avoid overheating of the sample, internal temperature control was used. Namely, the “heating over time” heating mode was chosen, where the heating time from room temperature to the required temperature in the range of 70–100 °C was fixed at 3.5 min. The best solvent type for MAE was evaluated on yield of phenolics and flavonoids detected quantitatively in given extracts using phytochemical screening assays. The optimization conditions for MAE were carried out for the best solvent type in a stepwise manner for the ratio of raw material/solvent (1/5, 1/10, or 1/15 (m/v)); time (15, 20, 25, 30, 35, 40, 45 or 50 min); temperature (70, 80, 90, or 100 °C); and number of repeats of the extraction process (1, 2, 3, 4, 5, or 6 times). All MAE runs were performed at a constant power of 50 W. The optimized conditions resulting in the highest extract yield using MAE were compared with the well-known and widely used conventional method of Soxhlet extraction in terms of energy efficiency.

#### Soxhlet extraction

Soxhlet extraction was performed using a Soxhlet apparatus (Sigma Aldrich, Steinheim, Germany) with an extractor capacity of 200 ml, a flask capacity of 300 ml, and a maximum operating power of 250 W. For the extraction, 10 g of dry crushed sample was placed into the extractor in cellulose extraction thimbles. Optimization of the conditions for the Soxhlet extraction was performed considering the number of operation cycles (thus, different operation times and energy consumption values) required to obtain the same degree of product (extract) yield, while the solvent type and ratio of raw material/solvent were the same as those under the optimized conditions for MAE.

The results were compared with the MAE technique in terms of the energy consumption required to produce the same amount of extract using the same optimal type of solvent reagent and initial ratio of materials.

#### Microwave-assisted activation (MAA)

MAA without the usage of any of the conventional activation agents (chemicals, CO2 or steam gases) in the atmosphere of the exhaust gas was used to process the biomass remaining after extraction with the aim of producing biochar—a valuable secondary product. After pre-drying at 110 ± 5 °C for 24 h, the biomass samples (solid residue after extraction) were subjected to MAA using a modified lab-scale cavity-type microwave oven (Milestone, Italy) with continuous output power and an operating microwave frequency of 2.45 GHz (wavelength 12.2 cm). The MAA process was carried out under a constant power of 600 W. The effect of operational parameters, namely, temperature (200–350 °C) and irradiation time (5–30 min), on the biochar yield and physical characteristics was investigated. Samples (100 g of pre-dried biomass) were placed in a ceramic crucible into the microwave oven cavity and heated at an average rate of 10 °C/min until the selected temperature of carbonization (200, 250, 300 or 350 °C) was achieved. Then, the temperature was held for different times (5–30 min). Finally, the carbonized biomass (biochar) was cooled to room temperature under N2 flow and kept in a desiccator until physical characterization. The yield was calculated as the ratio of the dry weight of the resultant biochar to the weight of the raw pre-dried biomass precursor. The resulting biochar characteristics were compared with the available values from the literature.

### Analytical methods

#### LC–MS analysis

The EtOH(30)/H2O(70) of C. edulis obtained by MAE were analysed by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) using an Ultimate 3000 SD (Thermo Scientific) and by HPLC coupled to high-resolution mass spectrometry (HPLC-HRMS) on an Ultimate 3000 RSL Cnano system (Thermo Scientific) interfaced with a quadrupole time-of-flight (QTOF) Impact II mass spectrometer equipped with an electrospray source (Bruker, Daltoniks). Chromatography separation was carried out on a Kinetics 5 μm C18 100 Å column (150 mm × 4.60 mm) or on a Kinetics 1.7 µm C18 100 Å LC column (150 × 2.1 mm) (Phenomenex, USA) at a constant temperature of 35 °C and flow rates of 0.300 mL min−1 and 0.150 mL min−1, respectively. The mobile phase consisted of 0.1% (v/v) formic acid in water (A) and 0.1% (v/v) formic acid in acetonitrile (B). The elution conditions were as follows: 5% B for 2 min; 5 to 30% B for 30 min; 30 to 100% B for 13 min; 100% B for 3 min; 100 to 5% 2 min; and finally, 5% B for 10 min.

The mass spectrometer was operated in ESI negative ion mode at high resolution. The optimized parameters were as follows: ion spray voltage, 2.5 kV; end plate offset, -500 V; nebulizer gas (N2), 2.8 bar; dry gas (N2), 8 L min−1; dry heater, 200 °C. Internal calibration was performed in high-precision calibration (HPC) mode with a solution of 10 mM sodium formate, which was introduced to the ion source via a 20 µL loop at the beginning of each analysis using a six-port valve. Sample analysis was performed by data-dependent acquisition (auto MSMS mode) in a m/z range of 50–1500 with a rate of 3 Hz and by a dynamic method with a fixed cycle time of 3 s. Data were processed using Data Analysis 4.1 software (Bruker Daltonics).

#### Identification of flavonoid glycosides. detection of fragmentation pathways

To denote the various fragment ions obtained by LC-MS analysis of the C. edulis extract, the nomenclature for flavonoid fragmentations proposed by Claeys and that for carbohydrate fragmentations proposed by Domon and Costello37 and subsequently expanded by Li and Claeys38 were adopted and used (Fig. 5). The labels i,JA and i,JB have been applied to define the primary fragment ions containing intact A and B rings, respectively, and the superscripts i and J indicate the number of broken C-ring bonds. The labels Ynand i,JXn denote glycoside flavonoids. The rupture of glycosidic bonds accompanied by hydrogen transfer gives rise to ions of Yn type, whereas i,JXn indicates ions formed by multiple cleavages involving the i,J bonds of carbohydrate units, where the subscript n indicates the number of interglycosidic bonds from aglycone.

#### Phytochemical screening assays

Phytochemical screening assays were performed to detect the total phenolic compound and flavonoid sub-family contents in the extract samples. A UV-Vis GBC 918 spectrometer was used to detect the target phytochemicals.

#### Total phenol determination

The total phenolic compounds were determined using Folin-Ciocalteu reagent39. Plant extract (100 μl, 0.01%), Folin-Ciocalteu reagent (500 μl) and sodium carbonate (2 ml, 2%) were thoroughly mixed and kept at room temperature for 30 min before measurements. The total phenolic concentration was determined by the absorbance at 720 nm using gallic acid as a standard at 10 to 200 μg/ml. y = 0.1572 x - 0.147, with R2 = 0.988.

#### Total flavonoid determination

Flavonoids were determined using the aluminium chloride colorimetric method40. A total of 250 μl of plant extract (0.01%) was mixed with 75 μl of NaNO2, 150 μl of AlCl3 (10%), 500 μl of NaOH (1 N) and 2.5 ml of distilled water. The mixture was held at room temperature for 5 min, after which the absorbance of the mixture was measured at 510 nm. The calibration curve was prepared using quercetin solutions at 10 to 120 μg/ml. y = 0.2832 × – 0.263, with R2 = 0.986.

#### Surface and texture characterization

The biochar was characterized by elemental and proximate analyses using an automatic CHNS-O elemental analyser and a Flash EATM 1112.

The porosity and surface parameters of the biochar samples were measured by means of low-temperature nitrogen adsorption isotherms at 77 K collected using a BET accelerated surface area and porosimetry analyser (Micromeritics ASAP 2010). Prior to adsorption testing, the samples were outgassed at 240 °C for 24 h under a pressure of 10−3 Pa.

The specific surface area was calculated using the BET equation41. The Dubinin-Radushkevich42 and Barrett-Joyner-Halenda (BJH)43 methods were applied to determine the micro- and mesopore volumes, respectively. The biochar point of zero charge (PZC) was obtained by acid–base titration44.

### Energy consumption calculation

The energy consumption (E) in kilowatt-hours (kWh) for a single extraction run is equal to the power P in watts (W) multiplied by the number of hours of extraction device usage (th) per extraction process and divided by 1000 watts per kilowatt, according to Eq. (1):

$${E}_{(kWh)}={P}_{(W)}\times {t}_{(h)}/{1000}_{(W/kW)}$$
(1)

### Total mass balance evaluation

The total mass balance for the integrated processing scheme was estimated via calculation of the yield of the primary valuable product—phytochemicals, from the extraction process—and the secondary valuable product—biochar, from biomass processing. Emphasis was also given to the estimation of the yield and mass balance of the flavonoid sub-family of phytochemical products extracted from C. edulis. The exact conservation law was used in the analysis of the system45.

The moisture content of the C. edulis leaf mass was calculated by measuring the mass while “wet” (fresh leaves) and that after drying and using Eq. (2):

$$\% Moisture=100\frac{{m}_{wet}-{m}_{dry}}{{m}_{wet}}$$
(2)

however, further calculations were performed on a dry matter (water-free) basis, i.e., with respect to the C Edulis leaf mass after pre-drying, which was considered to be 100%.

## Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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## Acknowledgements

This work was supported by the Associate Laboratory for Green Chemistry (LAQV), which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019); and the HORIZON 2020 EU Programme NANOMED project [grant number 734641 H2020-MSCA-RISE-2016].

## Author information

Ser.L., A.C.h. and S.L. conceived of the presented idea. A.P. realized general supervision of the work. Ser.L., M.C., N.A., A.L., A.C.h., S.L., and A.P. conceived and planned the experiments. Ser.L., O.Sh., O.L., M.C., A.L. and A.C.h. carried out the experiments. Ser.L., O.S.h., M.C., N.A., A.L., and A.C.h. contributed to the interpretation of the results. Ser.L. and S.L. took the lead in writing the manuscript with support from A.L. and M.C. All authors provided critical feedback and helped shape the research, analysis and manuscript.

Correspondence to Sergiy Lyubchyk.

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Lyubchyk, S., Shapovalova, O., Lygina, O. et al. Integrated Green Chemical Approach to the Medicinal Plant Carpobrotus edulis Processing. Sci Rep 9, 18171 (2019) doi:10.1038/s41598-019-53817-8