For baby odor analyses, noninvasive, stress-free sample collection is important. Using a simple method, we succeeded in obtaining fresh odors from the head of five newborn babies. These odors were chemically analyzed by two-dimensional gas chromatography coupled with mass spectrometry (GC × GC-MS), and compared with each other or with the odor of amniotic fluid from the baby’s mother. We identified 31 chemical components of the volatile odors from neonate heads and 21 from amniotic fluid. Although 15 of these components were common to both sources, there was an apparent difference in the GC × GC patterns between the head and amniotic fluid odors, so the neonate head odor might be individually distinct immediately after birth. Therefore, we made artificial mixtures of the major odor components of the neonate head and maternal amniotic fluid, and used psychological tests to examine whether or not these odors could be distinguished from each other. Our data show that the artificial odor of a neonate head could be distinguished from that of amniotic fluid, and that the odors of artificial head odor mixtures could be correctly discriminated for neonates within an hour after birth and at 2 or 3 days of age.
In mammals, odor cues emitted by newborn babies and/or mothers are essential to establish a mother-infant interaction at parturition1,2. Soon after birth the infants must initiate milk suckling to survive. In the mouse, maternal odors promote first suckling of neonates3. In humans, the sense of smell is arguably the most underestimated sense among our five fundamental senses but is known to have some social communicative functions4,5,6,7,8. As with other mammals, human neonates can also perceive environmental odors as cues to recognize aspects of the surrounding world and to seek their mothers. The olfactory system, which already functions in the fetus surrounded by the amniotic fluid, is one of the earliest emerging systems in fetal development9. Therefore, the odor of the amniotic fluid is thought to be a memorable odor not only for neonates but also for the fetus. It has been suggested that infants are attracted to or soothed by the odor of the amniotic fluid via olfactory learning during the fetal stage10,11 and later acquire various communication skills through reinforcement learning of favorability associated with their mothers12,13,14. They can memorize and be attracted to odors of their mother and mother-related odors such as the odors of milk and amniotic fluid11,12,13,14,15,16,17. At the same time, mothers and/or other adults may be aware of odors emitted by babies, and actively seek the smell of such odors in daily child-rearing18,19.
It has also been reported that human body odors are used as chemical cues for kin recognition20,21,22,23. Kaitz et al. (1987), Porter, Cernoch & McLaughlin, (1983) and Schaal et al. (1980) independently investigated the accuracy by which mothers can discriminate between their own baby and unrelated babies by odor cues derived from the babies24,25,26. The major findings of these experiments are consistent: within several days postpartum, mothers reliably identified their own neonate’s t-shirt by odor cues alone when it was presented along with t-shirts worn by other babies. Blindfolded mothers could recognize their offspring accurately when sniffing the heads of three infants, one of whom was their own offspring27. Moreover, Schaal and Marlier (1998) showed that both human mothers and fathers can discriminate the odors of two different samples of amniotic fluids, one from their own baby and the other from an unrelated baby28. Data suggest that human amniotic fluid carries individualized odor properties, leading to the hypothesis that it plays a role in the initiation of parent-infant interactions. Dubas et al. (2009) showed that both mother and father can discriminate between their own child and a control child by smell, and also made a preliminary assessment of whether olfactory discrimination is linked to odor preferences and parental investment29. Recently, Croy et al. (2017) used a study based on odor ratings to suggest that babies smell wonderful to their parents but teenagers do not30.
These earlier studies have highlighted the importance of olfactory cues in the formation and development of mother-infant relationships but (with one or two exceptions31,32) there has been no systematic investigation to analyze or identify the essential components of such odors serving as olfactory cues. In contrast, metabolites in human amniotic fluids have been investigated for medical purposes such as disease diagnosis. In almost all cases using gas chromatography with mass spectroscopy (GC-MS) for chemical analyses, amniotic fluid samples were pre-treated to stabilize volatile metabolites, to enable analysis of both volatile and nonvolatile substances together by the GC-MS system33,34,35. However, such analyses using liquid samples confound volatile odor components with nonvolatile water-soluble components.
There are few papers focusing on the chemical analysis of volatile odor components of human neonate heads and amniotic fluid. The focus of the present paper is on an accurate analysis of volatile chemicals in amniotic fluid and also the fresh odors of the newborn baby, both of which are sensed by the olfactory system.
Direct sampling is challenging, both technically and ethically, so in previous research on neonate odors, odor samples have been collected not directly from babies but indirectly from clothes such as t-shirts worn by the babies36,37,38,39. In the present study, we were able to analyze the volatile substances in fresh odor samples obtained directly from the heads of newborn babies using a stress-free method. However, in view of the delicate nature and ethics of sampling human neonates, sample numbers were limited for this pioneering study.
In addition, we carried out a sensory evaluation study with young adults, using an artificial mixture of 14 major components of volatile substances emitted by the neonate head within an hour of birth, 13 components from the head of babies 2 or 3 days postpartum, and 10 components from amniotic fluid obtained at the time of birth. Considering the psychological and/or physiological effects of the odor of amniotic fluid and neonates, which are probably among the earliest cues to promote mother-infant communication10,18,19,29,38,39, the combination of chemical analysis combined with sensory evaluation reported here is a potentially important contribution to research on early mother-infant bonding and communication.
Chemical identification of odor components of a baby’s head and the mother’s amniotic fluid
Odor samples were analyzed by GC × GC-MS, enabling discrimination of different spots which were superimposed in one-dimensional GC-MS. Figures 1 and 2 show representative contour plots as two-dimensional (2D) gas chromatograms for the head odor of a baby (Baby 1 in Table 1) and for the amniotic fluid odor of his mother (Mom 1 in Table 1). Here, the results of GC × GC-MS analyses focus on the volatile components of the odors. The contour plots for the baby’s head odor show inseparable spots of compounds of relatively longer retention times, indicating longer retention times than the spots shown in Fig. 1. Such inseparable spots mainly include unidentified hydrocarbons or their derivatives, which were presumed to be non-volatile for their retention times. In contrast, there should be no non-volatile substances present on the contour plots for the amniotic fluid odor because these samples were collected by the in-vitro headspace sampling method at room temperature and there was no direct contact with the amniotic fluid. Considering these conditions, the 2D gas chromatograms were naturally divided into volatile and nonvolatile parts, and data in Fig. 1 were used only from the volatile part (510–1080 s retention time).
There were 31 specific spots of volatile odor compounds obtained from the head of babies (Fig. 1, Top) and 21 from the amniotic fluid (Fig. 2, Top). In addition to the specific volatile odor compounds (white circles), siloxanes and their derivatives were detected. These are likely to be derived from the adsorbent beads (black circles without asterisks in Figs 1 and 2), or nonspecific contaminants derived from solvent- or synthetic resin-related materials (black circles with asterisks). The odors of both a newborn baby’s head (Fig. 1, Top) and amniotic fluid (Fig. 2, Top) contained 15 specific, commonly detected components, but the GC × GC pattern of volatile odors is apparently different between them. The controls (Figs 1 and 2, Bottom) indicate similar GC × GC patterns except for two spots of nonanal and decanal in Fig. 1, both of which are much weaker in the control (Fig. 1, Bottom) than the sample pattern (Fig. 1, Top). Siloxanes and their derivatives also are seen in both control patterns of Figs 1 and 2 (black circles without asterisks), although the control pattern of Fig. 1 has two such spots more than that of Fig. 2 (reason unknown).
From the total analytical results aggregated for all tested samples (five babies’ heads and two amniotic fluid samples), the 37 volatile odor components obtained are listed in Table 1. This table shows the area of well-separated m/z, which is corrected by subtracting the area of the corresponding peak if it appeared in the control. Table 1 shows that, except for limonene, volatile odor components from the baby’s head were commonly detected in all five babies tested, regardless of their odor sampling time after birth. They included large amounts of nonanal and other aldehydes (heptanal and octanal), and carboxylic acids (valeric, hexanoic, heptanoic, octanoic and nonanoic acids). The quantity of carboxylic acids gradually increased after birth. In particular, the amount of heptanoic acid was at the control level in Babies 1 and 2 (collected within an hour after birth), but markedly large amounts were detected in Babies 3–5 (sampled 2 or 3 days postpartum). In addition, several hydrocarbons were detected in the odors from both the baby’s head and the amniotic fluid.
Difference in the GC × GC pattern among odors of babies’ heads and amniotic fluids
Figure 3 shows the 37 identified odor compounds in the babies’ heads and amniotic fluids (compounds no. 1 to 37 in Table 1) plotted as norms in a 37-dimensional vector space including each odor sampled. The patterns show apparent differences in odor composition, with those obtained from a baby’s head (Babies 1–5) indicating relatively smaller amounts in substances of shorter retention times, while amniotic fluid (Moms 1 and 2) had larger amounts of substances of longer retention times. The samples of odors from the baby heads differ among each other more than do those obtained from amniotic fluid. The odor profiles of Babies 1 and 2, which were collected within an hour after birth, look less similar to each other than those of Babies 3–5 collected 2 or 3 days after birth.
The inset of Fig. 3 shows quantitative squared-differences in GC × GC pattern among the odor samples, with larger and smaller values suggesting correspondingly larger and smaller degrees of difference between pairs of odors. The head odor profile difference between Babies 1 and 2 (squared-difference = 0.40) was larger than that among Babies 3–5 (squared-differences = 0.03, 0.15 or 0.25). This difference was also similar to the difference between Baby 1 or 2 and Baby 3, 4 or 5 (squared-difference = 0.44 to 0.55) except between Babies 2 and 5 (squared-difference = 0.23). Although Moms 1 and 2 were the real mothers of Babies 1 and 2, respectively, the amniotic fluid odor difference between Moms 1 and 2 (squared-difference = 0.09) was much smaller than the head odor difference between Babies 1 and 2 (squared-difference = 0.40). The odor difference between the amniotic fluid of Moms 1 or 2 and the head of each of the babies (squared-difference = 1.33 to 1.70) was quite large regardless of the combination for mother-baby comparison.
The amniotic fluid odor contains some volatile hydrocarbons that were not detected in the baby’s head odors: 4-methylheptane, 2,4-dimethylheptane, and 2,4-dimethyl-1-heptene (Table 1). There were 15 components commonly detected both in the odors of a baby’s head and in the amniotic fluid, including 3 aldehydes (heptanal, octanal and nonanal) and 5 carboxylic acids (valeric, hexanoic, heptanoic, octanoic and nonanoic acids). The odors of Babies 1 and 2 contain carboxylic acids, including hexanoic, heptanoic, octanoic and nonanoic acids. The carboxylic acid content of the head odor of Baby 1 and Baby 2 was less than for that of Baby 3, 4 or 5 or the amniotic fluid odors of Moms 1 and 2.
Sensory evaluation of volatile odors from a baby’s head and from amniotic fluid
The evaluation experiment used three target odor samples (i.e., mixtures based on the mean odor components of the heads of Babies 1 and 2, heads of Babies 3–5, and amniotic fluids of Moms 1 and 2; see Supplemental Tables 1–3), and four test odors (the same as the target odor mixtures, plus odor-free solvent control). Participants were presented with one of the three target odors, and then provided with the four test odors. When the target odor was either the head odor mixture based on Babies 1 and 2 or that of Babies 3–5, the correct identification rate for all participants was above 70% (significantly different from the chance level of 25% by a binomial test; p < 0.05, p < 0.01 and p < 0.001 are indicated by *, **, and ***, respectively). The correct identification rates were also above 70% when data were analyzed separately for male and female participants. For amniotic fluid, the total correct identification rate was lower (55%) than for babies’ head odors for all participants but still significantly greater than chance. The performance by female participants was much better (73%) than by males (36%), whose performance was not significantly greater than the chance level (Fig. 4A).
These results were corroborated by a series of repeated-measures analysis of variance with the test odor sample as the independent variable. The repeated-measures ANOVAs were conducted separately for the three target conditions. As shown in Fig. 4B, the highest similarity ratings were associated with the matched-odor condition (i.e., the test odor sample was the same as the target odor sample). The main effect of test odor type was significant for the three analyses: F(3, 76) = 50.70, p < 0.001 for the Babies 1 and 2 condition; F(3, 76) = 54.51, p < 0.001 for the Babies 3–5 condition; F(3, 84) = 46.06, p < 0.001 for the amniotic fluid condition. A series of post-hoc tests with Tukey’s method indicated that the matched-odor similarity rating was significantly different from the unmatched-odor similarity ratings. When the target odor was “Babies 1 and 2,” participants perceived it as significantly more similar to the matched test odor of “Babies 1 and 2” (89.0%) than unmatched test odors (66.5% for Babies 3–5; 23.5% for Moms 1 and 2; 17.5% for solvent alone). The same pattern held for the other target odors, but with some exceptions. When the target odor was “Babies 3–5,” participants perceived it as significantly more similar to the matched test odor of “Babies 3–5” (89.0%) than the two unmatched test odors (37.5% for Moms 1 and 2; 15.0% for Solvent). However, its similarity to the unmatched test odor of “Babies 1 and 2” (75.5%) was not significantly different from the matched similarity (89.0%). When the target was “Moms 1 and 2,” participants perceived it as significantly more similar to the matched target of “Moms 1 and 2” (78.6%) than the two unmatched targets (61.4% for Babies 1 and 2; 11.4% for solvent alone). However, target similarity to the unmatched test odor of “Babies 3–5” (75.5%) was not significantly different from the matched similarity (78.6%). These results indicate that participants generally perceived the three target odors to be more similar to the matched test odors than the unmatched test odors.
We also conducted comparable repeated ANOVAs for each sex separately (see Fig. 4C,D). The results were mostly comparable with the analyses involving both sexes (Fig. 4B). However, probably due to the small sample size, a few additional comparisons that were significant in the main analyses (Fig. 4B) failed to reach the level of statistical significance. It is also noteworthy that the perceived similarity ratings between babies’ head odors (either Babies 1 and 2 or Babies 3–5 as target) and amniotic fluid odor (test) were relatively low (23.5% for target Babies 1 and 2; 37.5% for target Babies 3–5 in Fig. 4B). Nonetheless, the perceived similarity ratings between amniotic fluid odor (target) and babies head odors were modest (61.4% for test Babies 1 and 2; and 75.5% for test Babies 3–5 in Fig. 4B). Thus, asymmetric perceptions of similarity for amniotic fluid odor were notable in the present olfactory evaluation.
In spite of some unexpected patterns, then, on the whole the student evaluators were shown to be capable of discriminating the three types of odor samples from each other and from the control odor of solvent alone.
Chemical analyses of natural odors have been performed widely, and our understanding of volatile organic chemicals (VOCs) derived from various odor sources in natural environments, houses, foods, or even human bodies has greatly advanced40,41,42. Recent progress has relied much on methodological developments, such as the solid-phase micro-extraction (SPME) method combined with mass-spectrometry. Indeed, SPME is a useful odor adsorbing tool, but not applicable to odor sources as delicate as those of newborn babies. Even newborn babies try to avoid unpleasant treatments, and this renders it difficult to collect odors from neonates under stress-free conditions. It is for these reasons that few observations are available on the odors of newborn babies, and those that have been done have typically made use of the clothes used by babies instead of collecting the odors directly36,37,38,39.
In the present study, we successfully collected fresh odors of newborn babies immediately after birth by using MonoTrap silica beads. During odor sampling, no baby cried or fretted but remained rather relaxed or even sleeping in the bed or its mother’s arms. Thus, we conclude that our odor sampling method was successfully applicable even to newborn babies soon after birth.
Odor compounds such as those emitted from the human body, tend to change upon oxidization43. Indeed, as shown in Fig. 3, the head odors of babies 2 or 3 days after birth (Babies 3–5) tended to differ from those sampled within an hour after birth (Babies 1 and 2). It is noted, however, that without more extensive sampling it is difficult to differentiate the effects of odor changes attributable to oxidization from random differences among individual babies. Nevertheless, it was observed that the odor of the baby’s head, once trapped in the monolithic silica adsorbent, hardly changed in its GC × GC pattern following storage overnight in a refrigerator (unreported observations). A disadvantage of this method is the inevitable contamination of the odor samples by siloxanes and their derivatives because the MonoTrap beads are composed of monolithic silica. The GC data also included nonspecific peaks concerned with solvent- or synthetic resin-related contaminants, which were probably derived from the use of surgical gloves, etc. Hence, analysis of odor samples by GC × GC-MS (rather than GC-MS alone) is recommended to increase the resolution for detecting specific spots which may otherwise be hidden among those of contaminants.
Amniotic fluid provides the total environment for a fetus. Odors derived either from amniotic fluid or the body surface of the fetus can therefore be expected to be present at their interface. Thus, the odor on the body surface of a fetus and probably of a newborn baby soon after birth can be expected to have common components, at least in part, common with those of the amniotic fluid. However, the body surface of a newborn baby and the amniotic fluid are expected to contribute their own specific-odor components. As shown in Table 1, odor components specific to the baby’s head or the amniotic fluid presumably cause the characteristic differences in the GC × GC pattern (compare between Figs 1 and 2). The odor composition of neonates shows dissimilarities to those of both amniotic fluid and babies 2 or 3 days postpartum (Fig. 3). This suggests that the head odor of a newborn baby is not composed only of odor compounds lingering from the amniotic fluid. Also, Babies 3–5 had 14 compounds in common with Babies 1 and 2, suggesting that babies continuously secrete these 14 odor compounds, which are therefore detected even after the baby has been bathed several times.
As for the change of the odor composition of a baby’s head, aldehydes were maintained at mostly the same level, but carboxylic acids increased three-fold in the 2 or 3 days after birth. For example, nonanal was the most abundant odor component both in the neonate (Babies 1 and 2; average occupancy of peak area = 27%) and Babies 3–5 (2 or 3 days postpartum; average occupancy of peak area = 29%); while nonanoic acid increased five-fold during the few days after birth, which might be explained by oxidation of aldehydes following exposure of the neonate to air instead of the amniotic fluid. Although the odor compounds specific to the amniotic fluid were not likely to be derived from the baby’s body surface, some odor compounds might be transferred from the fetus to the amniotic fluid in utero. Generally, carboxylic acids are more easily dissolved into an aquatic environment than aldehydes, so those released from the fetus would be dissolved in the amniotic fluid. Thus, either one of both factors (transfer of carboxylic acids from fetus to the amniotic fluid; and oxidization of aldehydes after birth) might explain the quantitative change of carboxylic acids from Babies 1 and 2 to Babies 3–5 (see Table 1), which is reflected in the difference in GC × GC patterns between them (Figs 1–3).
It has been reported that body odors are used as olfactory cues for kin recognition. Many studies have suggested that the relationship between parents and their own babies attracting each other is based on mutual detection of odors specific to the other18,20,21,22,28,29. Even small differences in some specific components or their mixture pattern are possibly important for kin recognition of newborn babies by their parents, but this would require highly sensitive olfactory capability. In the present study, we found that the volatile component of odors from the heads of Babies 1 and 2 immediately after birth could reflect individual odor profiles. After that, the volatile odor composition changed, so that individual difference tended to decrease in a few days. Data in Fig. 3 show smaller odor differences among Babies 3–5 than that between Babies 1 and 2, suggesting that a baby can more strongly express its individuality soon after birth rather than a few days later. However, if newborn babies stay in a common nursery in a hospital with a standard environment, their body odors might tend to become uniform. This might explain why a larger odor difference occurred among newborn babies immediately after birth (square difference 0.40 in Fig. 3) rather than 2 or 3 days after birth (0.03–0.25). Figure 3 indicates that the square-difference between the head odors of Babies 1 and 2 (0.40) is within a similar range to that between any two head odors of Babies 1–5 (0.23–0.55). However, Fig. 4 suggests that the artificial mean odor mixture of Babies 1 and 2 can be discriminated from the artificial mean odor mixture of Babies 3–5. This suggests that two odor mixtures with a squared-difference in the range 0.23–0.55 can be discriminated, and therefore this would apply to the odors of Babies 1 and 2, the squared-difference between which is 0.40. At this stage, however, further discussion on baby odor individuality (or its role in kin recognition) is precluded in view of the relatively few odor samples from newborn babies available in the present study.
Not only kin recognition but also early mother-infant bonding and communication may be mediated by odors emitted from infants25,26,27,38. As humans are social species, infants might rely on attracting the attention of their mother, father, other relatives or other adults in situations where their survival is endangered. Considering the helplessness of the newborn human baby and the necessity of protection not only by its mother but also by family, community and society, it might be reasonable to expect that a newborn baby’s odor, even immediately after birth, could attract the attention of adults of various statuses. Thus, it may be worth investigating the sensory evaluation of infant odors by adults other than parents. The sensory evaluation experiment reported here was performed with young adults aged 18–24 y but they received no explanation about the odor, so this experiment was not keenly directed to mother-infant bonding responses or kin recognition. The results suggest that both female and male adults can readily distinguish the odor of a neonate’s head from that of a baby 2–3 days old (Fig. 4). This could be consistently explained by the chemical analyses of differences in the natural head odor among babies (Fig. 3).
It was interesting that female evaluators showed a higher rate of correct identifications than males for the amniotic fluid odor (Fig. 4A). There have been many reports suggesting that the female olfactory sense is better than that of males, a conviction that has achieved the status of an urban legend44. However, in olfactory discrimination tests where the participants are requested to decide which of the two or three odors is different, the data obtained are not consistent in terms of potential sex differences45,46, as seen also for the sensory evaluation tests reported in the present study (except for that on the amniotic fluid odor). Thus, there is presumably some reasonable explanation for this exceptional intersexual difference, but it is obscure at the present time. On the odor similarity of amniotic fluid to the baby’s head and of the baby’s head to the amniotic fluid, the answers obtained were asymmetric (Fig. 4B). This might be the result of some unknown psychological effect concerned, for example, with the presentation order of odor samples.
Needless to say, newborn babies are limited in their capacity for meaningful communication. However, they can cry and also emit their own odors even immediately after birth. Acoustic stimulation with the cry of a baby emotionally influences mothers, and it also seems very likely that the baby odors may have a psychological effect on mothers18,29. Okamoto et al. (2016) have noted that experience of detecting a baby’s odor by the mother induces feelings of care and attention directed towards her baby19. When a mother is detecting her baby’s odor, her brain activity is significantly increased in the bilateral prefrontal cortex39. Using fMRI, maternal status-dependent activity was found in the thalamus when research participants were exposed to the body odor of a newborn baby37. However, in all such cases, the odor components or odor pattern emitted by the baby that effectively induce such brain activity have yet to be identified.
For physiological or any other studies to progress, it is stressed here that noninvasive, stress-free collection and convincing analyses of a newborn baby’s odor are indispensable. Our chemical identification relied on referring to the NIST (National Institute of Standard and Technology) library, and on retention index searching for every odorant peak with standard compound peaks. It is also required to mimic the natural odor of a newborn baby precisely by experimenting with artificial odor mixtures. The present study came very close to concocting artificial odors mimicking natural odors, falling a little short of perfection because of a small number of unavailable or unidentified components (Supplemental Tables S1–S3).
Further refinements to the protocol in future studies will include confirmation of odor evaluator marital status, and information on previous experience of interaction with babies, timing of the menstrual cycle, etc47,48. These shortcomings preclude further fruitful discussion in the present paper. Nevertheless, there is useful consistency in the experimental data of the chemical analysis and sensory evaluation reported here. In future, if odor sampling from newborn babies could be conducted systematically, sample numbers could be optimized to include variables such as age of sampled babies for various days after birth, separate sampling of both sexes, and modes of delivery by natural birth or caesarean section, to more precisely investigate odor changes with age and other aspects such as the social effects of odors on kin-recognition.
Sample donors were 5 newborn babies delivered between August 18 and 21, 2017, in the hospital of Hamamatsu University School of Medicine. The participant mothers were assumed to have typical Japanese diets at home, although we obtained no individual information about food habits. These donor babies and mothers, who are native Japanese, were individually identified and in this study are referred to as Babies 1–5 and Moms 1 and 2. Baby 1 is the child of Mom 1 and Baby 2 the child of Mom 2. Babies 1 (male), 3 (female), 4 (male) and 5 (male) were delivered by the normal vaginal route, while Baby 2 (female) was delivered by caesarean section. The odor samples of Babies 1 and 2 were collected within 1 hour of birth. The body was carefully and gently wiped with clean towels before odor sampling. The odor samples of Babies 3 and 4 were collected 2 days after birth, and of Baby 5 collected 3 days after birth. The babies were bathed every day. Odor sampling was approved by the institutional review board of Hamamatsu University School of Medicine. We obtained informed consent from all participant mothers. All experiments were performed in accordance with the relevant guidelines and regulations of Hamamatsu University School of Medicine.
Odor sampling was performed with MonoTrap beads (RGPS TD; GL Sciences Inc., Tokyo Japan), which are composed of a monolithic silica adsorbent readily molded into cylindrical beads. These beads, when placed gently on a baby’s head inside a net bandage (Fig. 5), were totally ignored by the babies. For odor sampling from the baby’s head, five MonoTrap beads were wrapped with a small elastic net bandage (Shinsei Co., Ltd., Nara Japan; Fig. 5A,B) and placed as a lightly-fitting hat on the baby’s head (Fig. 5C). At the same time, we prepared the control samples by wrapping another five MonoTrap beads with a small elastic net bandage and placing them on a clean tissue paper (KimWipe; Nippon Paper Crecia Co., Ltd., Tokyo, Japan) for 20 min at room temperature. Manipulation during these steps was with the use of clean surgical gloves. After 20 minutes, the MonoTrap beads were transferred to 1.5 ml sample vials (Shimadzu Co., Kyoto, Japan), which were kept at 4 °C until subjected to the GC × GC-MS analysis.
For odor sampling from the amniotic fluid, immediately after delivery about 40 ml of amniotic fluid was collected by the attending obstetrician during delivery, 10 ml of which were placed in a glass bottle (40 ml, 22 mm diameter, 80 mm height GL Sciences Inc., Tokyo, Japan). Four MonoTrap beads were suspended from a stainless-steel shaft suspended from the septum in the lid of the bottle and were retained in the headspace of the glass bottle for 2 hours at room temperature (Fig. 5D). For the control odor sampling, another four MonoTrap beads were treated in the same way as for odor sampling from the amniotic fluid, but substituting distilled water for amniotic fluid. The beads were then kept in the sample vials at 4 °C until subjected to the GC × GC-MS analysis.
All preparations, such as wrapping the MonoTrap beads or setting them in the headspace for odor sampling, were done using surgical gloves in a clean, gently ventilated room with restricted entry under stable conditions of 20 °C and 30~40% relative humidity.
GC × GC-MS analysis
The GC × GC-MS analyses were conducted using a Pegasus 4D GC × GC-TOFMS system (Leco Co., Saint Joseph, MI, USA). A Handy TD portable thermal desorption system (TD265; GL Sciences Inc., Tokyo, Japan) was used to desorb sample odors adsorbed into the MonoTrap beads. One Monotrap bead was used for each analysis. The temperature program for thermal desorption was 40 °C for 0.1 min to 300 °C at 45 °C/min (final hold for 5 min) with a pre-desorption pressure of 180 kPa. Volatiles were transferred to a GC capillary column for separation at an inlet temperature of 250 °C. The primary column was a DB-1 MS column (30 m length × 250 µm i.d. × 0.25 µm film thickness; Agilent Technologies Inc., Santa Clara, CA, USA) and the secondary column was an InertCap 17 column (1.3 m length × 180 µm i.d. × 0.18 µm film thickness; GL Sciences Inc., Tokyo, Japan). Helium was used as carrier gas at a constant flow of 1.5 ml/min. The temperature program for the primary GC oven was 40 °C for 1 min, to 260 °C at 10 °C/min (final hold for 10 min). For the secondary GC oven, the temperature program was 50 °C for 1 min, to 270 °C at 10 °C/min (final hold for 10 min). The modulation period, hot-pulse duration, and cool time between stages were 3.0, 0.65, and 0.85 s, respectively. The transfer line to the Time-of Flight Mass Spectrometer (TOF-MS) detector was operated at 280 °C. The source temperature was 230 °C with a filament bias voltage of -70 eV. The data acquisition rate was 100 Hz in the mass range of m/z 35–500. Data were processed and visualized on 2D chromatograms using Leco Chroma TOF software (Leco Co., Saint Joseph, MI, USA). Individual components were first tentatively identified by referring to the NIST (National Institute of Standard and Technology) library, and more accurately identified by retention index searching with known standard substances, except for 8 of the 37 detected substances (Nos. 4, 10, 14, 28, 33, 34, 36 and 37 in Table 1) for which known standard chemicals were unavailable and/or retention indexes were not consistently determined.
Data analysis of difference among GC × GC patterns
Differences among the obtained GC × GC patterns were evaluated qualitatively, using the squared Euclidean distance between any two vectors as a criterion difference among the seven vectors obtained from the seven GC × GC patterns described in GC × GC-MS analysis. Each vector consists of 37 components chosen from its original pattern and is normalized to unit size prior to distance calculation. The number of vector dimensions is smaller than that of the original pattern because nonspecific contaminants (i.e. siloxanes, siloxane derivatives and solvent- or synthetic resin-related materials) were excluded from the analysis. The calculated distance ranges from 0.0 to 2.0 because the vectors have non-negative values in this analysis. The distance 0.0 indicates that the two vectors are identical; the value 2.0 indicates that the vectors are orthogonal.
Artificial odor mixing
Based on the 15 major odor components from the heads of Babies 1 and 2 and of Babies 3–5, and those from the amniotic fluid of Moms 1 and 2, their mean amounts were calculated and artificial odor mixtures to our specifications were obtained upon request to San-Ei Gen F.F.I., Inc. (Osaka, Japan). Supplemental Tables S1–S3 show the composition lists of each artificial mixture (see table captions for factors precluding the achievement of exact duplication). Sensory evaluation tests were conducted using these artificial mixtures diluted (by volume) to 1% by addition of 80% propylene glycol and 19% ethanol, as advised by San-Ei Gen F.F.I., Inc. (Osaka, Japan).
Sensory evaluation experiment
Sensory evaluations were conducted with 62 participants aged 18–24 years (31 male and 31 female students of Kobe University). The participants were assumed to be unmarried and not raising their own children (a reasonable assumption for this demographic group in Japan: e.g., in a previous study by one of the authors, none of 300 Japanese university/college students were married and/or had a child). The experiments were conducted in small group sessions (5 sessions with 10 participants, 1 session with 9, and 1 session with 3). All experiments were conducted on a blind basis, such that participants were unaware of the type or origin of the odor samples.
Participants were first presented with one of three kinds of artificial odor mixture (target odors) mimicking (1) the head odor of a neonate within 1 h postpartum (Supplemental Table 1); (2) the head odor of a baby 2 or 3 days postpartum (Supplemental Table 2); or (3) odor of amniotic fluid sampled at parturition (Supplemental Table 3). These target odors were impregnated into the tip of filter-paper strips (6 mm × 150 mm). The three artificial odor mixtures and solvent alone were presented to the participants 15 min. later and they were then asked to determine which of the four test samples was identical to the target odor they had smelled earlier. Participants were also required to rate their level of confidence in their decision and each odor sample’s similarity to the target sample, with perceived similarity rated on an 11- point scale at 10% intervals (0% = “not at all similar” to 100% = “very similar”). Each session, rather than each individual participant, was the unit of odor assignment (two sessions each were assigned to each of the three target odor samples). This experiment was approved by the Human Research Ethics Committee of the Graduate School of Humanities, Kobe University. All experiments were performed in accordance with the relevant guidelines and regulations of Kobe University.
The datasets analyzed during the current study are available from the corresponding author on reasonable requests.
Corona, R. & Lévy, F. Chemical olfactory signals and parenthood in mammals. Horm. Behav. 68, 77–90 (2015).
Lévy, F. & Keller, M. Olfactory mediation of maternal behavior in selected mammalian species. Behav. Brain Res. 200(2), 336–345 (2009).
Logan, D. W. et al. Learned recognition of maternal signature odors mediates the first suckling episode in mice. Curr. Biol. 22, 1998–2007 (2012).
Cupchik, G., Phillips, K. & Truong, H. Sensitivity to the cognitive and affective qualities of odours. Cognition & Emotion. 19(1), 121–31, https://doi.org/10.1080/0269993044100011 (2005).
de Groot, J. H. B., Semin, G. R. & Smeets, M. A. M. On the communicative function of body odors: A theoretical integration and review. Perspectives on Psychological Science 12(2), 306–324 (2017).
Ferdenzi, C., Coureaud, G., Camos, V. & Schaal, B. Human awareness and uses of odor cues in everyday life: Results from a questionnaire study in children. Int. J. Behav. Dev. 32(5), 422–431 (2008).
Lübke, K. & Pause, B. Always follow your nose: The functional significance of social chemosignals in human reproduction and survival. Horm. Behav. 68, 134–144, https://doi.org/10.1016/j.yhbeh.2014.10.001 (2015).
Shoup, M. L., Streeter, S. A. & McBurney, D. H. Olfactory comfort and attachment within relationships. J. Appl. Soc. Psychol. 38(12), 2954–2963, https://doi.org/10.1111/j.1559-1816.2008.00420.x (2008).
Browne, J. V. Chemosensory development in the fetus and newborn. Newborn & Infant Nursing Reviews. 8(4), 180–186 (2008).
Larkin, M. Smell of amniotic fluid soothes newborns. The Lancet 351, 1037 (1998).
Varendi, H., Christensson, K., Porter, R. H. & Winberg, J. Soothing effect of amniotic fluid smell in newborn infants. Early Hum. Dev. 51(1), 47–55 (1998).
Marlier, L., Schaal, B. & Soussignan, R. Neonatal responsiveness to the odor of amniotic and lacteal fluids: a test of perinatal chemosensory continuity. Child Dev. 69(3), 611–623 (1998).
Mizuno, K. Infantile Olfactory Learning. In Handbook of Behavior, Food and Nutrition (eds Preedy, V. R., Watson, R. R., Martin, C. R.) 119–132 (2011).
Varendi, H., Porter, R. H. & Winberg, J. Attractiveness of amniotic fluid odor: evidence of prenatal olfactory learning? Acta Paediatr. 85(10), 1223–1227 (1996).
Sullivana, R. M. & Toubas, P. Clinical usefulness of maternal odor in newborns: Soothing and feeding preparatory responses. Biol. Neonate 74, 402–408 (1998).
Vaglio, S. Chemical communication and mother-infant recognition. Communicative & Integrative Biology. 2(3), 279–281 (2009).
Wagner, S. et al. Infants’ hedonic responsiveness to food odours: A longitudinal study during and after weaning (8, 12 and 22 months). Flavour 2, 19 (2013).
Fleming, A. S. et al. Postpartum factors related to mother’s attraction to newborn infant odors. Dev. Psychobiol. 26(2), 115–132 (1993).
Okamoto, M., Shirasu, M., Fujita, R., Hirasawa, Y. & Touhara, K. Child odors and parenting: A survey examination of the role of odor in child-rearing. PLoSOne. 11(5), e0154392 (2016).
Ferdenzi, C., Schaal, B. & Roberts, S. C. Family scents: developmental changes in the perception of kin body odor? J. Chem. Ecol. 36(8), 847–854 (2010).
Porter, R. H. Olfaction and human kin recognition. Genetica. 104(3), 259–263 (1998).
Porter, R. H. & Moore, J. D. Human kin recognition by olfactory cues. Physiol. Behav. 27(3), 493–495 (1981).
Weisfeld, G. E., Czilli, T., Phillips, K. A., Gall, J. A. & Lichtman, C. M. Possible olfaction-based mechanisms in human kin recognition and inbreeding avoidance. J Exp Child Psychol. 85(3), 279–95 (2003).
Kaitz, M., Good, A., Rokem, A. & Eidelman, A. Mothers’ recognition of their newborns by olfactory cues. Dev. Psychobiol. 20(6), 587–91, https://doi.org/10.1002/dev.420200604 (1987).
Porter, R. H., Cernoch, J. M. & McLaughlin, F. J. Maternal recognition of neonates through olfactory cues. Physiol. Behav. 30(1), 151–4, https://doi.org/10.1016/0031-9384(83)90051-3 (1983).
Schaal, B. et al. Les stimulations olfactives dans les relations entre l’enfant et la mère [Olfactory stimulations in mother-child relations]. Reprod. Nutr. Dev. 20, 843–858 (1980).
Russell, M. J., Mendelson, T. & Peeke, H. V. S. Mothers’ identification of their infant’s odors. Ethol. Sociobiol. 4, 29–31 (1983).
Schaal, B. & Marlier, L. Maternal and paternal perception of individual odor signatures in human amniotic fluid – Potential role in early bonding? Biol. Neonate 74, 266–273 (1998).
Dubas, J., Heijkoop, M. & van Aken, M. A. A preliminary investigation of parent–progeny olfactory recognition and parental investment. Human Nature. 20(1), 80–92 (2009).
Croy, I., Frackowiak, T., Hummel., T. & Sorokowska, A. Babies smell wonderful to their parents, teenagers do not: an exploratory questionnaire study on children’s age and personal odor ratings in a Polish sample. Chem. Percept. 10(3), 81–87, https://doi.org/10.1007/s12078-017-9230-x (2017).
Loos, H. M. et al. Responsiveness of human neonates to the odor of 5α-Androst-16-en-3-one: A behavioral paradox? Chem. Senses 39, 693–703 (2014).
Vaglio, S., Minicozzi, P., Bonometti, E., Mello, G. & Chiarelli, B. Volatile signals during pregnancy: a possible chemical basis for mother–infant recognition. J. Chem. Ecol. 35, 131–139 (2009).
Antoshechkin, A. G., Golovkin, A. B., Maximova, L. A. & Bakharev, V. A. Screening of amniotic fluid metabolites by gas chromatography-mass spectrometry. J. Chromatogr. 489(2), 353–358 (1989).
Řimnáčová, L., Hušek, P. & Šimek, P. A new method for immediate derivatization of hydroxyl groups by fluoroalkyl chloroformates and its application for the determination of sterols and tocopherols in human serum and amniotic fluid by gas chromatography–mass spectrometry. J Chromatogr A. 1339, 154–67 (2014).
Kelley, R. I. Diagnosis of Smith-Lemli-Opitz syndrome by gas chromatography/mass spectrometry of 7-dehydrocholesterol in plasma, amniotic fluid and cultured skin fibroblasts. Clin Chim Acta. 236, 45–58 (1995).
Pandey, S. K. & Kim, K.-H. Human Body-odor components and their determination. Trend Anal. Chem. 30(5), 784–796, https://doi.org/10.1016/j.trac.2010.12.005 (2011).
Lundström, J. N. et al. Maternal status regulates cortical responses to the body odor of newborns. Front. Psychol. 4, 597 (2013).
Croy, I., Mohr, T., Weidner, K., Hummel, T. & Junge-Hoffmeister, J. Mother-child bonding is associated with the maternal perception of the child’s body odor. Physiol Behav. 198, 151–15 (2019).
Nishitani, S., Kuwamoto, S., Takahira, A., Miyamura, T. & Shinohara, K. Maternal prefrontal cortex activation by newborn infant odors. Chem. Senses. 39(3), 195–202 (2014).
Abou-El-Karam, S., Ratel, J., Kondjoyan, N., Truan, C. & Engel, E. Marker discovery in volatolomics based on systematic alignment of GC-MS signals: Application to food authentication. Anal. Chim. Acta. 991, 58–67 (2017).
de Lacy Costello, B. et al. A review of the volatiles from the healthy human body. J. Breath Res. 8(1), 014001 (2014).
Dutta, T. et al. The micro-environmental impact of volatile organic compound emissions from large-scale assemblies of people in a confined space. Environ. Res. 151, 304–312 (2016).
Haze, S. et al. 2-Nonenal newly found in human body odor tends to increase with aging. J. Invest. Dermatol. 116(4), 520–524 (2001).
Sorokowski, P. et al. Sex Differences in Human Olfaction: A Meta-Analysis. Front. Psychol. 10, 242, https://doi.org/10.3389/fpsyg.2019.00242 (2019).
Hummel, T., Kobal, G., Gudziol, H. & Mackay-Sim, A. Normative data for the “Sniffin’Sticks” including tests of odor identification, odor discrimination, and olfactory thresholds: an upgrade based on a group of more than 3,000 subjects. Eur. Arch. Oto Rhino Laryngol. 264, 237–243, https://doi.org/10.1007/s00405-006-0173-0 (2007).
Yang, L., Wei, Y., Yu, D., Zhang, J. & Liu, Y. Olfactory and gustatory function in healthy adult Chinese subjects. Otolaryngology 143, 554–560, https://doi.org/10.1016/j.otohns.2010.05.010 (2010).
Havlicek, J. & Lenochova, P. Environmental effects on human body odour. In Chemical Signals in Vertebrates (eds Hurst, J. L., Beynon, R. J., Roberts, S. C., Wyatt, T. D.) 11. Springer, New York, NY (2008).
Novákoválenka, L. M., Havlíček, M. & Roberts, S. C. Olfactory processing and odor specificity: a meta-analysis of menstrual cycle variation in olfactory sensitivity. Anthropological Review 77(3), 331–345, https://doi.org/10.2478/anre-2014-0024 (2014).
We are grateful to the babies and mothers who took part in this study. We thank medical staff of perinatal medical center of Hamamatsu University Hospital for the care of the babies and mothers. We thank GL Science Inc. (Tokyo, Japan) for the technical support on odor collection and application, Leco Japan Corporation (Tokyo, Japan) for technical support on chemical analyses by GC × GC-MS, and San-Ei Gen F.F.I., Inc. (Osaka, Japan) for technical support on artificial odor mixing and a part of the chemical analyses. This work was supported by JSPS Kakenhi Grant Number JP18KT0033. We thank Drs T.D. Wyatt and I.G. Gleadall for their helpful reading of a draft of this paper.
The authors declare no competing interests.
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Uebi, T., Hariyama, T., Suzuki, K. et al. Sampling, identification and sensory evaluation of odors of a newborn baby’s head and amniotic fluid. Sci Rep 9, 12759 (2019). https://doi.org/10.1038/s41598-019-49137-6
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