Abstract
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase that has been recognized as a therapeutic target for EML4-ALK fusion-positive nonsmall cell lung cancer (NSCLC) treatment using type I kinase inhibitors such as crizotinib to take over the ATP binding site. According to Shaw’s measurements, ALK carrying G1202R mutation shows reduced response to crizotinib (IC50 = 382 nM vs. IC50 = 20 nM for wild-type), whereas L1198F mutant is more responsive (IC50 = 0.4 nM). Interestingly, the double mutant L1198F/G1202R maintains a similar response (IC50 = 31 nM) to the wild-type. Herein we conducted molecular modeling simulations to elucidate the varied crizotinib sensitivities in three mutants carrying L1198F and/or G1202R. Both L1198 and G1202 are near the ATP pocket. Mutation G1202R causes steric hindrance that blocks crizotinib accessibility, which greatly reduces efficacy, whereas mutation L1198F enlarges the binding pocket entrance and hydrophobically interacts with crizotinib to enhance sensitivity. With respect to the double mutant L1198F/G1202R, F1198 indirectly pulls R1202 away from the binding entrance and consequently alleviates the steric obstacle introduced by R1202. These results demonstrated how the mutated residues tune the crizotinib response and may assist kinase inhibitor development especially for ALK G1202R, analogous to the ROS1 G2302R and MET G1163R mutations that are also resistant to crizotinib treatment in NSCLC.
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Introduction
Anaplastic lymphoma kinase (ALK) is a member of the insulin receptor protein–tyrosine kinase (RTK) superfamily, and has been recognized as an efficacious therapeutic target for the treatment of non-small cell lung cancer (NSCLC) because of its effect on the EML4-ALK fusion protein that occurs in patients with NSCLC1,2. The entire ALK protein structure includes an extracellular portion for ligand binding, a transmembrane segment, and an intracellular portion comprised of a juxtamembrane (JM) segment, a protein kinase domain, and a carboxyl terminal tail. In the basal condition, the ALK kinase domain remains in a uniquely intermediate state between its active and inactive forms. Specifically, the DFG motif adopts a DFG-in orientation, which is a common feature in active kinases3. F1271 points inward, which makes the ATP site available, and meanwhile, the aligned C1182, I1171, F1271, and H1247 residues form the R-spine that is usually assembled in the active state of kinase4,5. Yet, despite these two active state features, the A-loop adopts an autoinhibitory pose so that the entrance of the peptide substrate binding is blocked, resulting in the inactive state of ALK. To activate the ALK kinase function, the extracellular domain binds with a ligand such as midkine6 or pleiotrophin7, and dimerization of ALK is induced, which is followed by the phosphorylation of Y1278 on the A-loop by a partner ALK protein kinase domain. This generates a conformation change of the A-loop, and exposes the peptide substrate binding site, which turns on the phosphorylation function8,9,10,11.
Because ALK kinase dysregulation is involved in numerous diseases, including anaplastic large cell lymphomas, lung cancer, and neuroblastomas, many efforts have been made to develop ALK kinase inhibitors12,13,14,15,16,17,18; crizotinib19 and alectinib20 are among these. Crizotinib was approved by the FDA in 2011 for the purpose of treating ALK-positive NSCLC. Despite diseases being highly responsive to crizotinib treatment, roughly one in four patients tend to develop drug resistance within 1 to 2 years. According to the measurement by Friboulet et al., several drug-resistant mutations, such as G1202R (IC50 = 221 nM), S1206Y (IC50 = 124 nM), and G1269A (IC50 = 130 nM), are adjacent to the ATP/inhibitor-binding site in the kinase domain, cause steric hindrance to the inhibitors, but enable resumption of ALK activity (wild-type’s IC50 = 16 nM)21. By contrast, mutation L1198F, located near the ATP/inhibitor-binding site, enhances the sensitivity to crizotinib, achieving an IC50 = 0.4 nM compared with the WT’s IC50 = 20 nM as measured by Shaw et al.22. Moreover, Shaw et al. also determined that the L1198F/G1202R double mutant restores the sensitivity to crizotinib, achieving IC50 = 31 nM, which outperforms the IC50 = 382 nM measured for G1202R mutant. It is notable that mutation site G1202R is located in the solvent front of ALK kinase and confers remarkable resistance to numerous ALK inhibitors23. Moreover, G1202R in ALK is analogue to ROS1 G2302R and MET G1163R mutations and all these three exhibit drug resistance in NSCLC treatment. Therefore, understanding the drug resistance mechanism caused by G1202R in ALK certainly benefits drug developed for NSCLC.
Figure 1(A) illustrates a structure of ALK kinase domain with the L1198 and G1202 residues specified. The red segment between E1197 and G1202 is the hinge that plays an essential role in maintaining the relative movement between the N-lobe and C-lobe. Figure 1(B) depicts the surface presentation of a crizotinib-bound ALK complex structure where the purple and blue spots are L1198 and G1202, respectively. Figure 1(C) identifies the crizotinib structure with four ring moieties, namely piperidine, pyrazole, pyridine-2-amine, and halogenated benzene. Kinase inhibitors are divided into two types. Type I kinase inhibitors target kinases in the active state. They occupy the ATP pocket where the conserved DFG motif adopts a DFG-in orientation with phenylalanine pointing inward to spare the ATP binding site and also to participate in the R-spine assembly, which keeps the kinase domain in the proper movement to serve the enzyme function. A type II kinase inhibitor interacts with kinases in the inactive state in which DFG phenylalanine points outward and shrinks the ATP pocket, which generates an allosteric pocket in the back. The two types of kinase inhibitors have two types of binding channels24. Type I takes the ATP channel, whereas type II takes the allosteric channel. Crizotinib binds to the ATP pocket and is categorized as type I. ALK kinase is in the inactive state in its basal state, but its ATP pocket is available for the type I kinase inhibitors crizotinib and ceritinib17. In Fig. 1(B), the ATP channel is indicated by a solid-line arrow. Although the allosteric pocket is blocked by DFG phenylalanine and is not available in ALK, we circled the possible position and pinpointed the corresponding access channel with a dashed-line arrow for the purpose of comparison.
In this study we aimed to answer three questions: (i) how the mutation G1202R causes drug resistance to crizotinib, (ii) how mutation L1198F is more crizotinib-sensitive and superior to the WT, and (iii) how the double mutant L1198F/G1202R returns crizotinib sensitivity to a level similar to the WT’s. Recently, the role played by the binding affinity in drug development has often been criticized for it alone may not always serve as a sole descriptor to indicate drug efficacy25. On the other hand, there has been growing evidence to show that binding kinetics correlates better than binding affinity to drug efficacy26. Prior to our molecular modeling on the ALK systems, we have postulated that the replaced R1202 in G1202R mutant shall contribute stabilization toward the bound crizotinib, because as indicated in Fig. 1(A) G1202 is in the solvent front and the longer side chain of R1202 certainly provides more contact than G1202 does. Accordingly, the binding affinity of crizotinib in G1202R mutant should be better than that in WT and this introduces a conflict in the observed drug resistance by G1202R replacement. To resolve this conflict, we performed molecular dynamics (MD) simulations on the apo WT, G1202R mutant, L1198F mutant, and L1198F/G1202R mutant to investigate the minute conformation variance near the crizotinib binding pocket entrance so that we could correlate the observed drug accessibility to the varied IC50 values. Crizotinib-bound state of WT and the three mutants were also studied to evaluate their binding free energies. Moreover, umbrella sampling27 using the equilibrated structures extracted from the MD simulation trajectories for ALK-crizotinib complexes as initial structures was conducted to characterize the dissociation processes of crizotinib from the WT and the three mutants. On the basis of the obtained kinetic and thermodynamic observations, we provided valuable information applicable to drug development of ALK inhibitors.
Material and Methods
System setup
AMBER 12.0 software package28, assisted by ff99SB force field29, was utilized for this MD study. Ligand parameters were from the general AMBER force field (GAFF)30 and the Antechamber program. Moreover, the ligand atoms’ partial charges were obtained with electrostatic potential calculations at the HF/6-31G* level31 and assigned using AM-BCC protocol. The initial structure of the apo WT was retrieved from the X-ray crystallography solved structure in PDB code 3L9P32. With respect to the apo L1198F, G1202R, and L1198F/G1202R mutants, their initial structures were constructed through manual replacement of the mutated amino acid residues on the last conformation of the WT after the 50-ns production process. For the initial structures of the crizotinib-bound complexes, we used the crystal structure solved for the ALK-crizotinib complex in PDB code 5AAA22 as a template to superimpose over the last conformations of the apo WT, L1198F, and L1198F/G1202R trajectories after the 50-ns production procedure. Sodium ions were added to neutralize the studied systems: five ions for the apo WT, five ions for the apo L1198F mutant, four ions for the apo G1269R mutant, four ions for the apo L1198F/G1202R mutant, five ions for the WT–crizotinib complex, five ions for the L1198F–crizotinib complex, four ions for the G1202R-crizotinib complex, and four ions for the L1198F/G1202R–crizotinib complex. LEaP module was used to assign hydrogen atoms and protonation states of ionizable amino acid residue at neutral pH state. Each studied system was solvated in a cubic TIP3P water box with a 10 Å minimum solute-to-wall distance33 and the added water molecules underwent an energy minimization process comprising of the steepest descent algorithm (7500 steps) and the conjugate gradient algorithm (7500 steps) with a nonbonded cutoff of 8.0 Å. Furthermore, the same energy minimization process was carried out for another two times to relax the amino acid side chains (by restraining protein backbone atoms) and to relax the whole solvated system. For each studied system, we took the structure of the very end of the aforementioned three-staged energy minimization procedure as the initial structure for consecutive MD simulations. A standard protocol covering gradual heating (from 0 to 300 K in 60 ps and at 300 K for 40 ps), density equilibrium (at 300 for 100 ps), and equilibration (at 300 K for 800 ps) in isothermal isobaric ensemble setup (NTP with pressure of 1 atm and temperature of 300 K) was adopted prior to the production MD. As to the three ALK–crizotinib complex systems, each underwent a 50-ns simulation for conformation collection taken every 100 ps and for the subsequent binding free energy calculation (where only the last 10 ns trajectory was considered) and structural analysis. As to the four apo systems, we carried out two independent 50-ns production procedures and collected snapshots at a 100 ps interval for structural evaluation.
All the nonbonding interactions, such as short-range electrostatics and van der Waals interactions, within a cutoff distance 8 Å were considered. As to the long-range electrostatic interactions, the particle-mesh-Ewald method was applied34. All bonds involved in hydrogen atom to their equilibrium lengths were constrained by SHAKE algorithm35.
Binding free energy calculations
One hundred snapshots were extracted from the last 10 ns of the WT–crizotinib, L1178F–crizotinib, G1202R–crizotinib, and L1198F/G1202R–crizotinib trajectories to determine their ALK–crizotinib ΔGbind calculations. The MM/GBSA method has been taken as an accepted method to calculate the binding free energy between the receptor and its ligand36,37. The equation below is adopted:
That is, the free energy difference between the complex and the sum of ALK and crizotinib was considered as the binding free energy. Moreover, each Gmolecule term on the right-hand side of the equation is decomposed as follows:
Herein, the symbol \(\langle \ldots \rangle \) denotes the average for structures collected from an MD trajectory. The free energy Gmolecule term is composed of four parts: the gas-phase free energy EMM, the polar solvation free energy \({G}_{{\rm{solvation}}}^{{\rm{polar}}}\), the nonpolar solvation free energy \({G}_{{\rm{solvation}}}^{{\rm{nonpolar}}}\), and the entropy term −TS. Furthermore, the gas-phase free energy is to be calculated by the internal energy Einternal, the electrostatic energy Eelectrostatic, and van der Waals energy EvdW.
The polar solvation term is calculated using the Generalized Born model whereas the nonpolar solvation term is calculated with the surface-tension proportionality constant γ = 0.00542 kcal mol−1 Å−2 and the nonpolar free energy for a point solute β = 0.92 kcal mol−1 using the equation
where the solvent-accessible surface area, A, is varied by the molecule and calculated by the program. Moreover, the entropy term TS attributed by degree of freedom changes, including translational, rotational, and vibrational terms of the solute molecules, is estimated by normal mode analysis (NMA)38 using AMBER14’s nmode module. To save computational cost, 30 snapshots evenly extracted from the 40–50 ns production MD trajectories were used for the entropy calculations.
PMF calculation
The PMF calculation was achieved with umbrella sampling method27,39 by collecting multiple overlapping biasing potentials along the ATP-pocket dissociation pathway as the reaction coordinate40,41,42,43. WHAM44 was used to build the free energy profile along the reaction coordinate. Our reaction coordinate was set as the separation distance between the crizotinib C14 atom (pinpointed in Fig. 1(C)) and the ALK I1170 Cα atom (indicated in Fig. 1(A)). A separation distance ranging from 0 to 20 Å was used for the dissociation path, and the reaction coordinate was divided into 50 continuous windows. Each window considered a harmonic biased potential \({u}_{i}=\frac{1}{2}{k}_{i}{(r-{r}_{i})}^{2}\) with the force constant ki of 10 kcal/mol·Å2. The term ui is the biased potential in window i, r is the current position of reaction coordinate, and ri is the reference position in window i. Each simulation set used a 0.4-Å window length and for each window, we performed a 10-ns MD run to collect conformations with the temperature set at 300 K.
Results and Discussion
Binding energy analysis
To investigate the energetic contribution influenced by the minute conformational variance caused by mutated amino acid residues, we estimated the binding free energies using MM/GBSA for crizotinib-bound complexes. Figure 2(A) presents the root-mean-square deviation (RMSD) values of the heavy atoms of ALK and crizotinib along the 50-ns production duration for the four ALK-crizotinib complexes. The red curve depicting WT–crizotinib complex reached a stable state after 5 ns, fluctuating within 1.2 and 1.8 Å; the blue curve for L1198F–crizotinib complex became stable after 15 ns, fluctuating within 1.5 and 2.0 Å; the green curve for G1202R-crizotinib complex showed great magnitude indicating some fragments in great flexibility; as for the L1198F/G1202R–crizotinib complex, a jump was evident around 32 ns, and after the jump the purple curve became stable. To investigate the structural mobility of G1202R-crizotinib complex, we calculated different sets of RMSD values by excluding different segments of ALK and concluded that the N-terminal N1093-I1116 segment and the C-terminal D1389-E1400 segment contributed the flexibility. As shown in Fig. S1(A) in Supporting Information, two black parts in ALK structure indicate the locations of these two loose-end segments which are not in close contact with the core ALK structure. Figure S1(B) includes two RMSD curves for the whole G1202R-crizotinib complex (green curve) and the complex with deletion of the two aforementioned segments (green dashed curve) which is obviously stable within the entire production duration. In Fig. S1(C), we plotted this green dashed curve with the other three ALK-crizotinib complexes’ RMSD curves, and the fluctuating magnitudes are very alike. In Fig. 2(B) we plotted the RMSD values by considering only the heavy atoms within the 7 Å boundary of the bound crizotinib. The red, green, and purple curves for WT-, G1202R-, and L1198F/G1202R-crizotinib, respectively, reached a stable state less than 15 ns, whereas the blue curve for L1198F-crizotinib reached a stable state less than 25 ns. All these four curves suggested the structural stability around the binding pocket. In the following, we consider the last 10-ns trajectory for each complex by collecting 100 snapshots at a 100-ps interval to calculate ΔG and analyze structures.
As indicated in Fig. 3(A) for WT–crizotinib, the inhibitor is accommodated in a cleft between the N- and C-lobes, and is in a bent shape with the O27 atom (see Fig. 1(C)) as a pivotal point. The pyridine-2-amine ring moiety occupies the adenine pocket, approaches the ALK hinge, and forms two hydrogen bonds with E1197 and M1199 backbones. That is, crizotinib uses its pyridine nitrogen atom to link to M1199’s backbone amino hydrogen atom, and uses amine hydrogen to link to E1197’s backbone carbonyl oxygen atom. The piperidine ring protrudes toward the solvent front. Moreover, L1256 from the C-lobe and L1122, V1130, A1148, K1150, L1196, and G1202 from the N-lobe engage in hydrophobic interactions to clamp the inhibitor. For L1198F–crizotinib, depicted in Fig. 3(B), in addition to the aforementioned interaction, an extra hydrogen bond was discovered between K1150 and the fluorine atom on the halogenated benzene; π-stacking between F1198’s side chain and the middle pyridine ring was also present. As to the G1202R–crizotinib complex in Fig. 3(C), crizotinib maintains exactly the same binding mode as in the WT and the replaced R1202 uses its long chain to provide extra contact to secure the bound compound. In Fig. 3(D), the L1198F/G1202R–crizotinib complex contains all the interactions that occur in the WT-crizotinib complex, and the π-stacking from F1198’s aromatic ring and the long side chains of R1202 and K1150 add hydrophobic stabilization to the inhibitor.
Listed in Table 1 are the calculated binding free energies determined using the MM/GBSA method for the four studied complexes. Following the structural observation in Fig. 3, the binding affinities of the three mutants are all superior to WT–crizotinib (ΔGbind = −16.56 kcal/mol). Notably, the ΔGvdw column shows in both L1198F and G1202R mutants the mutated residues provide extra hydrophobic interaction (i.e., ΔΔGvdw = −46.38 − (−41.98) = −4.4 kcal/mol for L1198F relative to WT and ΔΔGvdw = −45.21 − (−41.98) = −3.23 kcal/mol for G1202R relative to WT). Furthermore, these extra hydrophobic interactions are synergized in the L1198F/G1202R–crizotinib complex which has ΔΔGvdw = −48.42 − (−41.98) = −6.44 kcal/mol. Although the rank of the binding free energies agree with the structural characteristics drawn in Fig. 3 where the mutated amino acids F1198 and/or R1202 boost binding affinity, it does not follow the trend of the experimentally measured IC50 values in the rightmost column. In the following we adopted kinetic point of view to explain the drug resistance by G1202R.
Structural variance of the binding pocket entrance
To monitor the MD trajectory convergence for the four apo ALK systems, the RMSD values of heavy atoms in the production duration were plotted as a function of time in Fig. 4. Each apo ALK underwent two separate MD simulations to increase the abundance of conformation collection. Except for L1198F/G1202R simulation-1 snaps occurring near 37 ns caused by flexible loops in Fig. 4(D), most of the curves fluctuated with variance within 1 Å. Accordingly, for each apo ALK system, we collected 100 snapshots for structural analysis at 10-ns intervals along the 50-ns trajectory of each of the two separate MD simulations.
Ribbon (in left panel) and surface (in right panel) representations in Fig. 5 provide the structural details centered at the crizotinib binding site on the four ALK systems. The left panel of Fig. 5(A) indicates that in the WT, L1198 is on the hinge and points outward, and the small G1202 sits underneath the crizotinib binding pocket. Thus, the crizotinib binding site is accessible as indicated in the surface representation given in the right panel. As for the L1198F mutant, the left panel of Fig. 5(B) displays that F1198’s bulky hydrophobic side chain still points outward (behaving similarly to L1198 in the WT) and is anchored from the top by Q1146 (on the β3 strand) and E1132 (on the β2 strand) through electrostatic interactions, a weak hydrogen bond with an occupancy of 19% (between the F1198 backbone NH group and the Q1146 backbone O atom), and an electrostatic π-anion interaction with an occupancy of 45% (between the F1198 benzene ring and the E1132 OE1/OE2 atom). Accordingly, the entrance for the crizotinib binding pocket remains clear, and the formation of two hydrogen bonds from hinges E1197 and G1202 toward the pyridine-2-amine ring moiety to secure the inhibitor is still feasible. The right panel of Fig. 5(B) illustrates that the cleft for crizotinib binding is available, and moreover, the cleft is wider than that of the WT, indicating some elevated ease in accommodating crizotinib. As for the G1202R mutant depicted in the left panel of Fig. 5(C), the elongated side chain of R1202 flips upward to interact with L1122 (on the β1 strand) through the hydrophobic interaction with their aliphatic long chains and a weak hydrogen bond (between the NH group of the R1202 side chain and the backbone O atom of L1122) with 37% occupancy. The β1 and β2 strands are elements of the P-loop that shield the ATP–crizotinib binding site. Accordingly, the right panel of Fig. 5(C) depicts that the entrance of the crizotinib pocket is blocked by the L1122–R1202 linkage. This explains how the inhibitory activity of crizotinib in G1202R is greatly reduced — crizotinib has difficulty getting in the binding site. Figure 5(D) presents how F1198 alleviates the drug resistance caused by R1202 in the L1198F/G1202R mutant. As indicated in the left panel, one hydrogen bond is formed between the F1198 backbone NH group and the Q1146 backbone O atom with 34% occupancy, and the π-anion interaction between F1198 and E1132 pulls down the β3 and β2 strands to introduce a linkage between E1132 and R1202 by means of electrostatic interaction. The right panel of Fig. 5(D) illustrates a conformation with the R1202–E1132 salt bridge where R1202’s long side chain tilts to the upper left and leaves the crizotinib binding pocket accessible; although the entrance opening is not as wide as in L1198F as depicted in the right panel of Fig. 5(B), it is similar to that in the WT as displayed in the right panel of Fig. 5(A).
To acquire more evidence of how the entrance opening in G1202R and in the L1198F/G1202R mutants are affected by the orientation of the R1202 long side chain, we monitored the R1202–L1122 and R1202–E1132 separation distances. The solid lines in Fig. 6(A) depict the separation distance fluctuation between the R1202 Cζ atom and the L1122 Cα atom in two independent MD trajectories of G1202R. Although the distance could reach a maximal value of 12 Å, it was more likely to remain under 6 Å, which is consistent with the aforementioned hydrogen bond formed between the R1202 side chain NH group and the L1122 backbone O atom. Illustrated in Fig. 6(B) is the separation distance between R1202 Cɛ atom and E1132 Cδ atom of G1202R. Because most of the distance is greater than 8 Å, the electrostatic interaction between the two charged ends rarely occurred. The separation distance between the R1202 Cζ atom and the L1122 Cα atom was also monitored in the L1198F/G1202R mutant, and plotted in Fig. 6(C) showing the R1202 is away from the L1122. Figure 6(D) depicts the separation distance between R1202 Cɛ atom and E1132 Cδ atom, populating between 4 to 15 Å and having a high likelihood of falling under 8 Å, which is consistent with the observation of the aforementioned R1202–E1132 salt bridge. To summarize, when G1202 is replaced by arginine with a long side chain, R1202 points upward and may fluctuate between L1122 (to the upper right and toward the P-loop) and E1132 (to the upper left and toward the hinge). In the G1202R mutant, R1202 more often remains close to L1122, as indicated by the two green curves in Fig. 6(E) that present the high population occurring at a short distance, instead of staying close to E1132, as the two green curves in Fig. 6(F) that present the high population occurring at a long distance illustrate. In the L1198F/G1202R mutant, R1202 movement is rather flexible, but tends to remain close to E1132 instead of L1122. To be more specific, as depicted in Fig. 6(E), the two purple lines for the R1202–L1122 distance in the L1198F/G1202R mutant shift to a longer distance range, compared with the two green curves for G1202R. In Fig. 6(F), the two purple curves peak at 4 Å (higher peak) and 12 Å (lower peak), indicating that R1202 spends some time near and some time away from E1132, and the salt bridge formed between them is likely on and off. Once the salt bridge is formed, R1202 is pulled leftwards to the hinge side leaves the entrance open.
Accordingly, the rankings of the binding pocket accessibility for the four studied ALKs from high to low are the L1198F mutant, the WT, the L1198F/G1202R mutant, and the G1202R mutant, which are consistent with their respective IC50 values: 0.4 nM for L1198F, 20 nM for WT, 31 nM for L1198F/G1202R, and 382 nM for G1202R. Moreover, it is likely that the association rate constants (kon) of these four ALKs follow the same rankings indicating L1198F mutant with an accelerated crizotinib-ALK binding process in comparison with the WT, whereas G1202R mutant with an decelerated binding process.
Dissociation pathway analysis using PMF
For many biological events that include protein-ligand association and dissociation and require microseconds or longer to complete, it is difficult to monitor the process with conventional MD simulations. Therefore, enhanced sampling methods such as adaptive biasing force45, steered molecular dynamics simulation46,47, and umbrella sampling48 are ideal tools to overcome the timescale problem. Herein, to investigate the unbinding kinetics, umbrella sampling was used to compute the free energy profile along the dissociation pathway of crizotinib escaping from the ATP pocket of the WT, L1198F, G1202R, and the L1198F/G1202R mutants. As plotted in Fig. 7, the barrier height of L1198F (24.38 kcal/mol at 9.8 Å separation distance) is higher than the other three — 18.98 kcal/mol at 12.9 Å for G1202R, 18.49 kcal/mol at 13.3 Å for L1198F/G1202R, and 12.49 kcal/mol at 9.4 Å for WT. This implies that the L1198F mutant binds crizotinib for the longest residence time among the four. By contrast, the WT has the shortest residence time, and the residence time for G1202R and for L1198F/G1202R are intermediate. The PMF convergence of the four systems is given in Fig. S2 in Supporting Information.
Conclusion
The ALK G1202R is analogous to the MET G1163R and ROS1 G2302R mutations that are also notorious for crizotinib resistance in NSCLC49,50. G1202 is a solvent-front residue and therefore when it is replaced with arginine, the orientation of the R1202 long side chain decisively regulates the accessibility of crizotinib, as determined in our structural analysis of the four apo ALK structures. Figure 5(C) illustrates that in the G1202R mutant R1202 points toward the P-loop and gets fastened by L1122, which seriously narrows the ATP channel opening. However, in Fig. 5(D) for the L1198F/G1202R mutant, R1202 points toward the hinge segment and interacts with E1132, which results in the ATP channel entrance experiencing less effect. It has been reported that the mutation G1202R also confers high-level resistance to alectinib, which was developed to be a more potent ALK inhibitor to combat various crizotinib-resistant ALK mutations such as L1196M and C1156Y51,52,53. Therefore, it is likely that the R1202 residue employs the same mechanism to cause drug resistance on type I kinase inhibitors, associating ALK kinase through the ATP channel.
To summarize our findings for the differential crizotinib sensitivity in the ALK mutants carrying G1202R and/or L1198F, instead of using the calculated binding free energies, we focused on the kinetic profiles of crizotinib binding to the four ALKs plotted onto the on-off-rate map in Fig. 8. The map is plotted with an association rate (kon) versus a dissociation rate (koff) where the parallel diagonal lines represent iso-affinity lines54. Points distributed alone a given iso-affinity line come with the same ratio of kon and koff values; strong affinity binding events appear in the upper left corner (with high kon and low koff values) whereas the weak affinity binding events appear in the lower right corner (with low kon and high koff values). Compared with the WT, the L1198F mutant possesses a larger kon value and a smaller koff value because the binding channel is more accessible for association (Fig. 5(B)) and the boundary height is higher for dissociation (Fig. 7). Altogether, these effects place the L1198F point to an upper iso-affinity line with an improved drug efficacy relative to the WT’s. As to the G1202R mutant, the shrunken ATP channel opening greatly decreases kon (Fig. 5(C)) and the dissociation boundary height slightly higher than that in the WT (Fig. 7) decreases koff. There two effects move the G1202R point to a lower iso-affinity line with a decreased drug efficacy. In the L1198F/G1202R mutant, koff is slightly reduced with a similar decreased magnitude as in G1202R (due to the similar barrier height for both G1202R and L1198F/G1202R mutants in Fig. 7) and kon is also decreased, as indicated by the entrance opening width appearing slightly narrower than the WT (Fig. 5(D)). The two effects offset each other and consequently L1198F/G1202R point stays in the same iso-affinity line in which the WT is located.
Lately there have been several theoretical studies centering on the drug resistance mechanisms caused by mutations in ALK45,55,56. Sun et al. studied three crizotinib-resistant mutants including L1152R, G1202R, and S1206Y by comparing their PMFs with WT’s45. They calculated crizotinib binding free energies and concluded structural difference of the four ALK-crizotinib complexes. Entropy influence introduced by P-loop, which is in close contact of the bound crizotinib, was discovered. He et al. studied alectinib-bound WT, I1171N, V1180L, and L1198F complexes, where these three mutants are alectinib-resistant55. Binding free energies and complex structural stability were evaluated to elucidate the drug resistance mechanisms. Li et al. studied different affinities of crizotinib and lorlatinib in WT, and three mutants covering L1198F and/or C1156Y56. They simulated crizotinib-, lorlatinib-, and ATP-bound ALKs and discussed how the replaced amino acid residue(s) affect the binding pocket and pinpointed the H1124 plays an essential role. In this presented work, we carried out simulations on WT and three MTs covering L1198F and/or G1202R. Both apo and crizotinib-bound ALKs were simulated. With the apo ALK data, we examined the accessibility of the binding site entrance and further ranked their kon values; with crizotinib-bound ALKs, we compared their binding free energies and conducted PMF calculations to rate their koff values. We believe the concluded comparison made for the ratios of kon/koff highlights the novelty of this study.
We made findings concerning the structural and kinetic interplay of ALK and crizotinib, and hopefully these results can be used to assist the rational design of ALK inhibitors to conquer the problem of mutations.
References
Roskoski, R. Anaplastic lymphoma kinase (ALK): Structure, oncogenic activation, and pharmacological inhibition. Pharmacol Res 68, 68–94 (2013).
Roskoski, R. Jr. Anaplastic lymphoma kinase (ALK) inhibitors in the treatment of ALK-driven lung cancers. Pharmacol Res 117, 343–356 (2017).
Tong, M. & Seeliger, M. A. Targeting conformational plasticity of protein kinases. ACS Chem Biol 10, 190–200 (2015).
Azam, M., Seeliger, M. A., Gray, N. S., Kuriyan, J. & Daley, G. Q. Activation of tyrosine kinases by mutation of the gatekeeper threonine. Nat Struct Mol Biol 15, 1109–1118 (2008).
Kornev, A. P., Taylor, S. S. & Ten Eyck, L. F. A helix scaffold for the assembly of active protein kinases. Proc Natl Acad Sci USA 105, 14377–14382 (2008).
Stoica, G. E. et al. Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types. J Biol Chem 277, 35990–35998 (2002).
Stoica, G. E. et al. Identification of anaplastic lymphoma kinase as a receptor for the growth factor pleiotrophin. J Biol Chem 276, 16772–16779 (2001).
Donella-Deana, A. et al. Unique substrate specificity of anaplastic lymphoma kinase (ALK): development of phosphoacceptor peptides for the assay of ALK activity. Biochemistry 44, 8533–8542 (2005).
George, R. E. et al. Activating mutations in ALK provide a therapeutic target in neuroblastoma. Nature 455, 975–978 (2008).
Tartari, C. J. et al. Characterization of some molecular mechanisms governing autoactivation of the catalytic domain of the anaplastic lymphoma kinase. J Biol Chem 283, 3743–3750 (2008).
Bossi, R. T. et al. Crystal structures of anaplastic lymphoma kinase in complex with ATP competitive inhibitors. Biochemistry 49, 6813–6825 (2010).
Heuckmann, J. M. et al. ALK mutations conferring differential resistance to structurally diverse ALK inhibitors. Clin Cancer Res 17, 7394–7401 (2011).
Bresler, S. C. et al. ALK mutations confer differential oncogenic activation and sensitivity to ALK inhibition therapy in neuroblastoma. Cancer Cell 26, 682–694 (2014).
Hallberg, B. & Palmer, R. H. The role of the ALK receptor in cancer biology. Ann Oncol 27(Suppl 3), iii4–iii15 (2016).
Katayama, R. Drug resistance in anaplastic lymphoma kinase-rearranged lung cancer. Cancer Sci 109, 572–580 (2018).
Wu, P., Nielsen, T. E. & Clausen, M. H. Small-molecule kinase inhibitors: an analysis of FDA-approved drugs. Drug Discov Today 21, 5–10 (2015).
Wu, P., Nielsen, T. E. & Clausen, M. H. FDA-approved small-molecule kinase inhibitors. Trends Pharmacol Sci 36, 422–439 (2015).
Roskoski, R. Jr. Classification of small molecule protein kinase inhibitors based upon the structures of their drug-enzyme complexes. Pharmacol Res 103, 26–48 (2016).
Cui, J. J. et al. Structure Based Drug Design of Crizotinib (PF-02341066), a Potent and Selective Dual Inhibitor of Mesenchymal-Epithelial Transition Factor (c-MET) Kinase and Anaplastic Lymphoma Kinase (ALK). J Med Chem 54, 6342–6363 (2011).
Sakamoto, H. et al. CH5424802, a selective ALK inhibitor capable of blocking the resistant gatekeeper mutant. Cancer Cell 19, 679–690 (2011).
Friboulet, L. et al. The ALK inhibitor ceritinib overcomes crizotinib resistance in non-small cell lung cancer. Cancer Discov 4, 662–673 (2014).
Shaw, A. T. et al. Resensitization to Crizotinib by the Lorlatinib ALK Resistance Mutation L1198F. N Engl J Med 374, 54–61 (2016).
Hatcher, J. M. et al. Discovery of Inhibitors That Overcome the G1202R Anaplastic Lymphoma Kinase Resistance Mutation. J Med Chem 58, 9296–9308 (2015).
Yang, L.J. et al. Steered Molecular Dynamics Simulations Reveal the Likelier Dissociation Pathway of Imatinib from Its Targeting Kinases c-Kit and Abl. PLoS One 4 (2009).
Bruce, N. J., Ganotra, G. K., Kokh, D. B., Sadiq, S. K. & Wade, R. C. New approaches for computing ligand-receptor binding kinetics. Curr Opin Struct Biol 49, 1–10 (2018).
Pan, A. C., Borhani, D. W., Dror, R. O. & Shaw, D. E. Molecular determinants of drug-receptor binding kinetics. Drug Discov Today 18, 667–673 (2013).
Kastner, J. Umbrella sampling. Wiley Interdiscip Rev -Comput Mol Sci 1, 932–942 (2011).
Case, D.A. et al. AMBER 12, University of California, San Francisco. AMBER 12. (2012).
Hornak, V. et al. Comparison of multiple Amber force fields and development of improved protein backbone parameters. Proteins 65, 712–725 (2006).
Wang, J., Wolf, R. M., Caldwell, J. W., Kollman, P. A. & Case, D. A. Development and testing of a general amber force field. J Comput Chem 25, 1157–1174 (2004).
Jakalian, A., Jack, D. B. & Bayly, C. I. Fast, efficient generation of high-quality atomic charges. AM1-BCC model: II. Parameterization and validation. J Comput Chem 23, 1623–1641 (2002).
Lee, C. C. et al. Crystal structure of the ALK (anaplastic lymphoma kinase) catalytic domain. Biochem J 430, 425–437 (2010).
Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. J Chem Phys 79, 926–935 (1983).
Darden, T., York, D. & Pedersen, L. Particle mesh Ewald: An N·log(N) method for Ewald sums in large systems. J. Chern. Phys. 98, 10089 (1993).
Ryckaert, J.-P., Ciccotti, G. & Berendsen, H. J. C. Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes. J. Comput. Phys. 23, 327–341 (1977).
Rastelli, G., Del Rio, A., Degliesposti, G. & Sgobba, M. Fast and Accurate Predictions of Binding Free Energies Using MM-PBSA and MM-GBSA. J Comput Chem 31, 797–810 (2010).
Onufriev, A., Bashford, D. & Case, D. A. Exploring protein native states and large-scale conformational changes with a modified generalized born model. Proteins 55, 383–394 (2004).
Brooks, B. & Karplus, M. Harmonic dynamics of proteins: normal modes and fluctuations in bovine pancreatic trypsin inhibitor. Proc Natl Acad Sci USA 80, 6571–6575 (1983).
St-Pierre, J. F., Karttunen, M., Mousseau, N., Rog, T. & Bunker, A. Use of Umbrella Sampling to Calculate the Entrance/Exit Pathway for Z-Pro-Prolinal Inhibitor in Prolyl Oligopeptidase. J Chem Theory Comput 7, 1583–1594 (2011).
Kang, C. M., Liu, D. Q., Wang, X. Y., Yu, R. L. & Lv, Y. T. The unbinding studies of vascular endothelial growth factor receptor-2 protein tyrosine kinase type II inhibitors. J Mol Graph 59, 130–135 (2015).
Sun, H. Y. et al. Revealing the favorable dissociation pathway of type II kinase inhibitors via enhanced sampling simulations and two-end-state calculations. Sci Rep 5 (2015).
Kong, X.T. et al. How Does the L884P Mutation Confer Resistance to Type-II Inhibitors of JAK2 Kinase: A Comprehensive Molecular Modeling Study. Sci Rep 7 (2017).
Pan, P. et al. Combating Drug-Resistant Mutants of Anaplastic Lymphoma Kinase with Potent and Selective Type-I(1/2) Inhibitors by Stabilizing Unique DFG-Shifted Loop Conformation. ACS Cent Sci 3, 1208–1220 (2017).
Chodera, J. D., Swope, W. C., Pitera, J. W., Seok, C. & Dill, K. A. Use of the weighted histogram analysis method for the analysis of simulated and parallel tempering simulations. J Chem Theory Comput 3, 26–41 (2007).
Sun, H. Y., Li, Y. Y., Li, D. & Hou, T. J. Insight into Crizotinib Resistance Mechanisms Caused by Three Mutations in ALK Tyrosine Kinase using Free Energy Calculation Approaches. J Chem Inf Model 53, 2376–2389 (2013).
Capelli, A. M. & Costantino, G. Unbinding Pathways of VEGFR2 Inhibitors Revealed by Steered Molecular Dynamics. J Chem Inf Model 54, 3124–3136 (2014).
Patel, J. S., Berteotti, A., Ronsisvalle, S., Rocchia, W. & Cavalli, A. Steered Molecular Dynamics Simulations for Studying Protein-Ligand Interaction in Cyclin-Dependent Kinase 5. J Chem Inf Model 54, 470–480 (2014).
You, W. & Chang, C. A. Role of Molecular Interactions and Protein Rearrangement in the Dissociation Kinetics of p38alpha MAP Kinase Type-I/II/III Inhibitors. J Chem Inf Model (2018).
Engstrom, L. D. et al. Glesatinib Exhibits Antitumor Activity in Lung Cancer Models and Patients Harboring MET Exon 14 Mutations and Overcomes Mutation-mediated Resistance to Type I MET Inhibitors in Nonclinical Models. Clin Cancer Res 23, 6661–6672 (2017).
Drilon, A. et al. A Novel Crizotinib-Resistant Solvent-Front Mutation Responsive to Cabozantinib Therapy in a Patient with ROS1-Rearranged Lung Cancer. Clin Cancer Res 22, 2351–2358 (2015).
Ignatius, O. S. H. et al. Next-generation sequencing reveals a Novel NSCLC ALK F1174V mutation and confirms ALK G1202R mutation confers high-level resistance to alectinib (CH5424802/RO5424802) in ALK-rearranged NSCLC patients who progressed on crizotinib. J Thorac Oncol 9, 549–553 (2014).
Kodama, T., Tsukaguchi, T., Yoshida, M., Kondoh, O. & Sakamoto, H. Selective ALK inhibitor alectinib with potent antitumor activity in models of crizotinib resistance. Cancer Lett 351, 215–221 (2014).
Song, Z., Wang, M. & Zhang, A. Alectinib: a novel second generation anaplastic lymphoma kinase (ALK) inhibitor for overcoming clinically-acquired resistance. Acta Pharm Sin B 5, 34–37 (2015).
Karl, A. & Markku, D. H. Replacing affinity with binding kinetics in QSAR studies resolves otherwise confounded effects. J Chemometrics 20, 370–375 (2007).
He, M., Li, W., Zheng, Q. & Zhang, H. A molecular dynamics investigation into the mechanisms of alectinib resistance of three ALK mutants. J. Cell. Biochem. 119, 5332–5342 (2018).
Li, J. et al. L1198F Mutation Resensitizes Crizotinib to ALK by Altering the Conformation of Inhibitor and ATP Binding Sites. Int J Mol Sci 18 (2017).
Acknowledgements
The authors are grateful for the financial support provided by the Ministry of Science and Technology in Taiwan with grant number 104-2815-C-390-005-B.
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Performed the simulations: Y.C.C., B.Y.H., H.W.C. Conceived the study: B.Y.H., C.N.Y. Wrote the manuscript: C.N.Y.
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Chuang, YC., Huang, BY., Chang, HW. et al. Molecular Modeling of ALK L1198F and/or G1202R Mutations to Determine Differential Crizotinib Sensitivity. Sci Rep 9, 11390 (2019). https://doi.org/10.1038/s41598-019-46825-1
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DOI: https://doi.org/10.1038/s41598-019-46825-1
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