Smokeless tobacco (SLT), a cause of potentially preventable diseases, has a diverse chemical composition encompassing toxicants as well as potent carcinogens. Though the chemical profile of SLT products has been analyzed earlier, this information is not available in a comprehensive and easily accessible format. Hence, there is an imperative felt need to develop a one-stop information source providing inclusive information on SLT products. SLTChemDB is the first such database that makes available detailed information on various properties of chemical compounds identified across different brands of SLT products. The primary information for the database was extracted through extensive literature search, which was further curated from popular chemical web servers and databases. At present, SLTChemDB contains comprehensive information on 233 unique chemical compounds and 82 SLT products. The database has been made user-friendly with facility for systematic search and filters. SLTChemDB would provide the initial data on chemical compounds in SLT products to various tobacco testing laboratories. The database also highlights research gaps and thus, would be a guide for researchers interested in chemistry and toxicology of SLT products. With regular update of information in the database, it shall be a valuable evidence base for policymakers to formulate stringent policies for SLT control.
Consumption of SLT is a global menace, estimated to account for approximately 0.65 million deaths per year1. Epidemiological studies indicate a significant role of SLT products in cancers, stroke, nervous and reproductive disorders1,2,3,4,5,6. A recent in-silico study indicated significant role of toxic chemical compounds in the diseases caused by SLT products7. Worldwide, there are different forms of SLT products available ranging from simple tobacco to complex products having many additives and flavoring agents. Reports suggest that the chemical composition of a tobacco plant gets altered significantly during the curing and processing of SLT products8. Many chemical compounds in the form of non-tobacco plant materials (like areca nut), humectants, flavoring agents and alkaline agents are also introduced to enhance the attractiveness and addictiveness of the SLT products8.
The chemical compounds identified so far in SLT products have been classified broadly into tobacco alkaloids, tobacco specific nitrosamines (TSNAs), volatile N-nitrosamines, N-nitrosamino acids, polycyclic aromatic hydrocarbons (PAHs), radionucleotides, metals, metalloids, pesticide residues, humectants, alfatoxins and mycotoxins9,10,11,12,13,14,15,16,17. TSNAs like N′-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL); PAHs like benzo[a]pyrene (B[a]P), radionuclides and alfatoxins have been classified as potent carcinogens by the International Agency for Research on Cancer (IARC)6,18,19. Nicotine, the predominant tobacco alkaloid and precursor of TSNAs, is also the cause of addictiveness associated with tobacco use20,21,22. Many independent studies have estimated the amount of nicotine, TSNAs, PAHs in various SLT products. Although existing studies estimated the chemical composition in a variety of products, there is a lack of adequate distribution and compilation of this pertinent information.
For effective control of SLT-attributable diseases, it is imperative to identify the chemical compounds present in SLT products, estimate their toxicity and study their specific role in diseases. No attempts have yet been made to compile data about the chemical compounds in SLT products. To the best of our knowledge, this study is the first attempt wherein we have collected and compiled vital details about the chemical information, physicochemical properties, biological information, toxicological information and distribution of chemical compounds present in SLT products. SLTChemDB is a one- stop information source crosslinked to various popular chemical databases like PubChem, ChemSpider and ChEMBL.
SLTChemDB is a comprehensive database of all the chemicals identified by testing various SLT products. Presently, the database contains comprehensive information about 233 chemical compounds (+2 mixtures of these compounds) and 82 SLT products. We also provide brand-wise chemical composition, pH, moisture, free nicotine and tobacco content of 41 SLT products. Figure 1 briefly explains all the information available in SLTChemDB. The database contains information about chemical composition, pH, moisture, mode of intake, free nicotine, tobacco content and country-wise information of SLT products along with Biological, Toxicological and Physico-chemical information of chemical compounds.
Out of the 233 chemical compounds, chemical information like canonical SMILES and IUPAC name of 224 compounds were taken from PubChem23 and ChemSpider24. 3D structures/Canonical SMILES taken from PubChem/ChemSpider were converted to 2D using Open Babel25 (for more details refer to Supplementary Table 1). Structures of 5 compounds (unavailable on PubChem/ChemSpider) were self-drawn and SMILES were generated using the tool provided on the SLTChemDB website. Structures of 4 chemical compounds (2 PAH, 1 Coumarin and 1 Radionucleotide) could not be generated due to lack of complete chemical information. Canonical SMILES were used to calculate properties like polar surface area, number of donor and acceptor hydrogen bonds, molecular weight, molecular formula. Further, canonical SMILES was used to predict toxicological information using pkCSM web server26. Information about protein targets of 38 chemicals was extracted from ChEMBL27. Complete statistics of the chemical compounds is shown in Table 1.
As per the International Agency for Research on Cancer (IARC) classification of carcinogens18, 69 compounds out of the 233 compounds were classified various carcinogenic groups. Out of 69 classified compounds, 7 compounds (Formaldehyde, Beryllium, Arsenic, Cadmium, N-nitrosonornicotine, 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone, Benzo[a]pyrene) were classified as Carcinogenic to humans (IARC Group 1), 8 were classified as Probably Carcinogenic to humans (IARC Group 2 A), 26 were classified as Possibly Carcinogenic to humans (IARC Group 2B) and 28 were classified as not classifiable as per the carcinogenicity to humans (IARC Group 3) [Fig. 2].
Among the 233 identified chemical compounds, major categories included PAH (32) followed by Radionucleotides (30), Metal/Metalloids (21), Additives (14) and others as shown in Fig. 3.
SLTChemDB holds immense utility for various stakeholders viz. researchers and policymakers by providing a one stop information source on chemical constituents of SLT products. SLTChemDB is the first such database that contains raw data useful for tobacco testing laboratories across the globe. Information from the database can also be used to identify the products and brands having minimum concentration of carcinogenic compounds. As an example, among different SLT products amount of NNN varies from 0.0132 µg/g in Rapè tobacco (Brand: Rapé Guarany Cristal) to 3085 µg/g (dry weight) in Toombak (Brand: N/A, Country Sample Source: Sudan). The amount of 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone varies from as low as 0.004 µg/g (wet weight) in Mawa (Brand: Mawa) to as high as 7870 µg/g (dry weight) in Toombak (Brand: N/A, Country Sample Source: Sudan). The amount of Benzo[a]pyrene varies from <0.0001 µg/g in Chewing tobacco (Brand: Click Eucalyptus) to as high as 0.198 µg/g in Moist Snuff (Brand: Kodiak Premium Wintergreens).
Also, the database highlights research gaps by providing product-wise list of identified chemical compounds. Thus, SLTChemDB will formulate the evidence base and initial data depicting the need for regulation and periodic testing of chemical constituents of SLT products.
SLTChemDB Web Interface
SLTChemDB has a very simple and user-friendly interface. Extensive search options using various tools have been provided, explained briefly as below:
This search option allows the users to search SLTChemDB in a very simple way using various keywords. User can search comprehensive information about SLT products and their chemical compounds using the options provided in the Search tab. This function has been depicted in Supplementary Fig. 5.
Advance search allows user to build complex queries using logical operators like “AND” and “OR” to search across various fields. The advanced search has been explained in Supplementary Fig. 6.
The available fields for Simple and Advanced search are depicted in Table 2.
Structure based search allows the user to derive information about chemical compounds by providing its structure either by drawing it or uploading a SMILES structure or mol file. User can select from three search types: Substructure/Exact search, Topological fingerprint-based search and MACCS key based search to generate results. Tanimoto coefficient depicting the structural similarity is displayed against each search result. Structure based search takes place using RD kit28. Structures are visualized using JSmol29. More information about the results of this search function is explained in Supplementary Fig. 7.
Using this option, user can compare the composition of different chemicals analyzed between available SLT products, brands and/or countries. This function has been explained in Supplementary Fig. 8 with example.
The current version of SLTChemDB contains information 233 chemical compounds and 82 SLT products. All information about SLT products and their chemicals is stored in seven tables. The following browse tabs are provided:
Physicochemical Information: This tab displays the compound name, molecular weight, Log P, hydrogen bond donors, hydrogen bond acceptors, polar surface area and links to other chemical databases of all the chemical compounds.
Biological information: This tab displays the list of proteins targeted by the of all the chemical compounds.
Toxicological information: This tab displays AMES toxicity, BBB permeability, Caco2 permeability, CNS permeability, CYP2D6 inhibitor, Fraction unbound in humans, Hepatotoxicity, Intestinal absorption in humans, Max. Tolerated dose in humans, P-glycoprotein substrate, Renal OCT2 substrate, Skin Permeability, Total Clearance, VDss in humans and water solubility of all the chemical compounds.
Chemical Composition: This tab displays information about the types of SLT products, brands, amount of chemicals, country from which sample was taken, country in which sample was tested along with the reference of the study from which information was taken.
Product Composition: This tab displays information about the brands, pH, moisture content, tobacco content and free nicotine content of SLT products.
Country-wise Information: This tab displays information about the availability of SLT products in different countries.
For the first-time user, the help tab can provide comprehensive detail about the methods to search and browse through the website to retrieve the desired information. This tab gives the list of information that can be accessed through the different search functions. Screenshots depicting the help tab are included in Supplementary Figs 1–10.
The statistics page contains table depicting the overall statistics of SLTChemDB database. It also contains table with the results of toxicological (ADMET) analysis of chemical compounds. A comprehensive list of all the SLT products and their brands with the number of chemicals identified in each brand.
Option to download all the data in.csv format will be available soon.
Update of SLTChemDB
The database shall be updated regularly to incorporate newly added research on this topic. Chemico-toxicological information on more SLT products shall also be included, whenever available in an authenticated form. Additionally, the database also provides an option to the user to submit his/her own information using the submission form available at SLTChemDB website. However, such data shall be authenticated by our team before inclusion in the database.
With the widespread use of SLT products, there is an enhanced rate of mortality and morbidity associated with SLT use. Since the health effects of SLT are attributed to its chemical constituents, it is essential to study in detail the chemical profile of various products. This research is hindered by the lack of easily available information on chemical composition of SLT products in a readily usable format. The situation is made more complex by the wide variation in chemical profile across brands of same product and within batches of a brand. Hence, SLTChemDB has been developed as the first comprehensive data repository of chemical, biological and toxicological information about chemical compounds identified across various brands of SLT products.
This database holds promise as an invaluable resource for various stakeholders viz. researchers and policymakers by providing a one-stop information source on chemical profiling of SLT products. For instance, SLTChemDB contains information about 222 Moist Snuff brands. Among them, the number of chemicals identified varies from as high as 44 in Copenhagen to just 1 in other brands like Husky Long Cut Wintergreen, Husky Long Cut Natural etc. With this information, SLTChemDB will highlight the existing gaps in testing of SLT products. Thus, this database is likely to be a valuable resource for the researchers with interest in chemical profiling of SLT products.
Since the levels of various chemicals vary widely between SLT products and also within brands of a particular products30, the creation of this database assumes importance being a valuable source for comparison between products and brands of a product. As an example, the amount of N-nitrosonornicotine (NNN) in all the moist snuff samples tested from United States varied from 0.71 µg/g to 64 µg/g across different brands. Within one moist snuff brand from United States, Grizzly, the amount of N-nitrosonornicotine (NNN) ranged between 2.64 µg/g to 11.1 µg/g and while within Skoal it varied from 0.76 µg/g (wet weight) to 42.6 µg/g (wet weight). This information from SLTChemDB shall provide evidence-base to policy makers to form stringent policies on regulation of toxic contents in SLT products. We would like to mention one limitation, that since the information on chemical composition of brands has been retrieved from published literature, a few brands in the database do not carry a name due to lack of this information in the concerned papers12,31,32,33,34.
SLT research is currently a changing arena with ongoing research on various aspects. SLTChemDB has been developed as an activity of WHO FCTC Global Knowledge Hub on Smokeless Tobacco established in ICMR-NICPR. This database shall be regularly updated to incorporate future information to maintain the comprehensibility of this database. In addition, some future directions may include incorporation of metabolites of chemicals present in SLT products and validation of the toxicological and biological information (estimated in-silico till now) through in-vivo and in-vitro methodologies.
Data collection and compilation
The information about chemical compounds present in SLT products was extracted through extensive search of peer-reviewed literature like papers, reports and monographs from PubMed and Google Scholar using various combinations of keywords (Table 3).
A total of 821 articles were collected which were filtered on basis of availability of full text and data on testing of SLT products. Information about the classification and composition of chemical compounds along with mode of intake, pH and moisture of SLT products was extracted from 85 published articles.
Data was compiled to obtain information about the physicochemical properties from renowned chemical databases like PubChem24 and Chemspider25. Further, the information about biological targets was extracted from large scale bioactivity database ChEMBL26. Each compound was classified into various carcinogenic groups as per the IARC classification of carcinogens27,28,29,30. Toxicological information of each chemical compound was calculated using pkCSM server31.
Database framework and web interface
Primary data involves information about classification and composition of chemical compounds along with mode of intake, pH and moisture of SLT products. This information was extracted from peer reviewed published articles.
Physicochemical properties of all the identified chemical compounds were extracted from PubChem database. Biological information (protein targets) of each chemical compound was extracted from ChEMBL database. Canonical SMILES structure of chemical compounds taken from PubChem was used for calculation of toxicological properties using pkCSM web server.
The database is freely available at bic.icmr.org.in/sltchem
Sinha, D. N. et al. Global burden of all-cause and cause-specific mortality due to smokeless tobacco use: systematic review and meta-analysis. Tob. Control 27, 35–42 (2018).
Boffetta, P. & Straif, K. Use of smokeless tobacco and risk of myocardial infarction and stroke: systematic review with meta-analysis. BMJ 339, b3060 (2009).
Rickert, W. S. et al. Chemical and toxicological characterization of commercial smokeless tobacco products available on the Canadian market. Regul. Toxicol. Pharmacol. 53, 121–133 (2009).
Borgerding, M. F., Bodnar, J. A., Curtin, G. M. & Swauger, J. E. The chemical composition of smokeless tobacco: A survey of products sold in the United States in 2006 and 2007. Regul. Toxicol. Pharmacol. 64, 367–387 (2012).
Alpert, H. R., Koh, H. & Connolly, G. N. Free nicotine content and strategic marketing of moist snuff tobacco products in the United States: 2000–2006. Tob. Control 17, 332–338 (2008).
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Smokeless Tobacco and Some Tobacco-specific N-Nitrosamines (2007).
Bhartiya, D. et al. In-silico study of toxicokinetics and disease association of chemicals present in smokeless tobacco products. Regul. Toxicol. Pharmacol. 95, 8–16 (2018).
National Cancer Institute and Centers for Disease Control and Prevention, Bethesda, M. Smokeless Tobacco and Public Health: A Global Perspective. (U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Institutes of Health, National Cancer Institute 2014).
Gupta, P. C., Murti, P. R. & Bhonsle, R. B. Epidemiology of cancer by tobacco products and the significance of TSNA. Crit. Rev. Toxicol. 26, 183–198 (1996).
Brunnemann, K. D., Scott, J. C. & Hoffmann, D. N-nitrosomorpholine and other volatile N-nitrosamines in snuff tobacco. Carcinogenesis 3, 693–696 (1982).
Hoffmann, D., Brunnemann, K. D., Prokopczyk, B. & Djordjevic, M. V. Tobacco‐specific, N. ‐nitrosamines and ARECA‐ derived N ‐nitrosamines: Chemistry, biochemistry, carcinogenicity, and relevance to humans. J. Toxicol. Environ. Health 41, 1–52 (1994).
Djordjevic, M. V., Brunnemann, K. D. & Hoffmann, D. Identification and analysis of a nicotine-derived N-nitrosamino acid and other nitrosamino acids in tobacco. Carcinogenesis 10, 1725–1731 (1989).
Stepanov, I. et al. Analysis of 23 Polycyclic Aromatic Hydrocarbons in Smokeless Tobacco by GC/MS. Chem. Res. Toxicol. 23, 66–73 (2010).
McAdam, K. et al. Analysis of hydrazine in smokeless tobacco products by gas chromatography-mass spectrometry. Chem. Cent. J. 9, 13 (2015).
McAdam, K. et al. Comprehensive survey of radionuclides in contemporary smokeless tobacco products. Chem. Cent. J. 11, 131 (2017).
Dhaware, D., Deshpande, A., Khandekar, R. N. & Chowgule, R. Determination of Toxic Metals in Indian Smokeless Tobacco Products. Sci. World J. 9, 1140–1147 (2009).
Pappas, R. S. Toxic elements in tobacco and in cigarette smoke: inflammation and sensitization. Metallomics 3, 1181–98 (2011).
Thun, M. J., Jemal. A. IARC Classification of Carcinogens. Holland-Frei Cancer Medicine. (Hamilton (ON): BC Decker 2003).
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans VOLUME 85 Betel-quid and Areca-nut Chewing and Some Areca-nut-derived Nitrosamines (2004).
Campain, J. A. Nicotine: Potentially a Multifunctional Carcinogen? Toxicol. Sci. 79, 1–3 (2004).
Benowitz, N. L., Hukkanen, J. & Jacob, P. Nicotine Chemistry, Metabolism, Kinetics and Biomarkers. Nicotine Psychopharmacol. 192, 29–60 (2009).
Benowitz, N. L. Nicotine addiction. N. Engl. J. Med. 362, 2295–303 (2010).
Kim, S. et al. PubChem Substance and Compound databases. Nucleic Acids Res. 44, D1202–13 (2016).
Pence, H. E. & Williams, A. ChemSpider: An Online Chemical Information Resource. J. Chem. Educ. 87, 1123–1124 (2010).
O’Boyle, N. M. et al. Open Babel: An open chemical toolbox. J. Cheminform. 3, 33 (2011).
Pires, D. E. V., Blundell, T. L. & Ascher, D. B. pkCSM: Predicting Small-Molecule Pharmacokinetic and Toxicity Properties Using Graph-Based Signatures. J. Med. Chem. 58, 4066–72 (2015).
Gaulton, A. et al. ChEMBL: a large-scale bioactivity database for drug discovery. Nucleic Acids Res. 40, D1100–7 (2012).
RDKit. Available at, http://www.rdkit.org/ (Accessed: 13th April 2018).
Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. & Sussman, J. L. JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem. 53, 207–216 (2013).
McNeill, A., Bedi, R., Islam, S., Alkhatib, M. N. & West, R. Levels of toxins in oral tobacco products in the UK. Tob. Control 15, 64–67 (2006).
Brunnemann, K. D., Genoble, L. & Hoffmann, D. N-Nitrosamines in chewing tobacco: an international comparison. J. Agric. Food Chem. 33, 1178–1181 (1985).
Österdahl, B. G., Jansson, C. & Paccou, A. Decreased levels of tobacco-specific N-nitrosamines in moist snuff on the Swedish market. J. Agric. Food Chem. 52, 5085–5088 (2004).
Brunnemann, K. D., Qi, J. & Hoffmann, D. Chemical profile of two types of oral snuff tobacco. Food Chem. Toxicol. 40, 1699–1703 (2002).
Orisakwe, O. E., Igweze, Z. N., Okolo, K. O. & Ajaezi, G. C. Heavy metal hazards of Nigerian smokeless tobacco. Tob. Control 23, 513–517 (2014).
Moher, D., Liberati, A., Tetzlaff, J. & Altman, D. G. & PRISMA Group. Preferred Reporting Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement. J. Clin. Epidemiol. 62, 1006–1012 (2009).
Special thanks to Amitesh Kumar Sharma, Indian Council of Medical Research for graphics support in the database. This work was supported by two projects: Data Management Laboratory, ICMR-NICPR (Project No. BIC/14(01)/TF/2014) and WHO FCTC Global Knowledge Hub on Smokeless Tobacco (Reference No. 2016/643768-0).
The authors declare no competing interests.
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Kaur, J., Sharma, A., Kumar, A. et al. SLTChemDB: A database of chemical compounds present in Smokeless tobacco products. Sci Rep 9, 7142 (2019). https://doi.org/10.1038/s41598-019-43559-y