Abstract
A novel disposable electrochemical biosensor based on immobilized calf thymus double-stranded DNA (dsDNA) on the carbon-based screen-printed electrode (SPE) is developed for rapid biorecognition of carrageenan by using methylene blue (MB) redox indicator. The biosensor protocol for the detection of carrageenan is based on the concept of competitive binding of positively charged MB to the negatively charged dsDNA and carrageenan. The decrement in the MB cathodic peak current (ipc) signal as a result of the released MB from the immobilized dsDNA, and attracted to the carrageenan can be monitored via differential pulse voltammetry (DPV). The biosensor showed high sensitivity and selectivity to carrageenan at low concentration without interference from other polyanions such as alginate, gum arabic and starch. Calibration of the biosensor with carrageenan exhibited an excellent linear dependence from 1–10 mg L−1 (R2 = 0.98) with a detection limit of 0.08 mg L−1. The DNA-based carrageenan biosensor showed satisfactory reproducibility with 5.6–6.9% (n = 3) relative standard deviations (RSD), and possessing several advantages such as simplicity, fast and direct application to real sample analysis without any prior extensive sample treatments, particularly for seaweeds and food analyses.
Similar content being viewed by others
Introduction
Carrageenans are used in a variety of commercial applications because of their excellent physical properties, especially as thickening, stabilizing and gelling agents in dairy food products such as frozen desserts. Besides, carrageenan is also a very important component in pharmaceutical formulations and cosmetics1,2,3,4. Carrageenans are high molecular weight sulfated polysaccharides that composed of the repeating disaccharide units with alternating 3-linked β-D-galactopyranose and 4-linked α-galactopyranose or 3,6-anhydro-α-galactopyranose (AnGal)5. There are basically three types of industrially relevant carrageenans i.e. κ-(kappa), ι-(iota) and λ-(lambda) carrageenans (Fig. 1), which are classified according to their specific molecular structures, including their sulfation patterns and the presence or absence of 3,6-anhydro-α-galactopyranose on 4-linked α-galactopyranose5,6. κ-carrageenan’s molecular structure was reported as alternating 3-linked β-D-galactose 4-sulfate and 4-linked AnGal units. The ι-carrageenans have an additional sulfate group on C2(O) of the AnGal moiety, thus resulting in two sulfates per disaccharide repeating unit. The λ-carrageenans have three sulfate groups per disaccharide unit with the third sulfate group available at the C6 position of the 4-linked residue5.
The carrageenan content in food is usually around 0.01–1.00% of the total weight of food. In view of the complexities in chemical compositions of foods, they often require extensive sample preparation and purification procedures in order to eliminate interferences prior to analysis of carrageenan can be carried out7,8,9,10 using conventional methods such as chromatographic techniques11,12,13,14,15,16. Furthermore, methods such as precipitation followed by gravimetric sulphate quantification, necessitates a large amount of carrageenan samples in order to obtain sufficient quantity of sulphate for analysis17, and this makes rapid carrageenan analysis difficult.
Colorimetric techniques can also be applied for the determination of total carrageenans by using cationic dyes, which form complexes with carrageenan, and measured with an optical system e.g. spectrofluorometer or spectrophotometer. Colorimetric assay is sensitive towards the degree of sulphation, and it gives different responses towards different types of carrageenans7. Shirai and co-workers18 have revealed that metachromatic behaviour of methylene blue (MB) cationic dye, which induced by the polyanions was dependent on the type of interaction involved such as electrostatic interaction, hydrophobic interaction and interaction of π-electrons.
MB interacts electrostatically with carrageenan at low concentrations through cationic alkylamino functional group of MB and anionic ester sulphate group in the carrageenan molecule. This basic dye has been used since 1930 by Ewe19 for the identification and differentiation between carrageenan and other natural gums from plants. Graham20 used MB for quantitative assay of carrageenan. Soedjak21 reported a new strategy for colorimetric determination of carrageenans by using MB stain, and this method becomes the most useful one until the present time1. Apart from MB reagent, some other cationic dyes such as alcian blue22 and acridine orange23 have also been employed for the determination of carrageenan via colorimetric and spectrofluorometric methods, respectively. Electrochemical interrogation of carrageenan has been reported by using potentiometric titration technique. The electrochemical sensor was prepared by immobilizing dinonylnaphthalene sulfonate (DNNS) and tridodecylammonium chloride (TDMAC) ionophore in the polyurethane or poly(vinyl chloride) membrane containing o-nitrophenyloctylether (o-NPOE) plasticizer, and protamine or poly-L-arginine titrant was used as a titrant24,25. Recently, a new electrocatalytic analysis of carrageenans in seawater was reported based on the catalytic hydrogen evolution (CHE) reaction on the mercury electrode via adsorptive transfer constant current derivative chronopotentiometric stripping technique, whereby λ-, ι- and κ-carrageenans were distinguished based on the nature of their respective accelerated faradaic peaks at negative potential26.
The development of a variety of portable, rapid, and sensitive DNA biosensors have rapidly flourished for clinical, forensic, environmental and pharmaceutical applications due to their easy DNA testing procedures and providing immediate result on-the-spot27,28,29. In view of the innate net negative charge along the sugar-phosphate backbone of DNA molecule, and the well-established accumulation of MB with the double-stranded DNA (dsDNA), in this paper, we introduce a novel voltammetric DNA biosensor for carrageenan detection based on the competitive electrostatic interaction of dsDNA and carrageenan towards binding with MB. Figure 2 illustrates the schematic competitive binding of cationic MB to negatively charged dsDNA and carrageenan as well as the pictorial representation of the electrochemical voltammetric DNA device and the electrical setup involved.
The dsDNA of calf thymus was adsorbed on the screen-printed carbon paste electrode (SPE) at open-circuit potential, and intercalated with MB redox marker. Upon addition of carrageenan into the measurement cell, the cationic MB molecules released from the immobilized dsDNA due to the higher affinity of MB to form complex with carrageenan polyion. The decline in the reduction signal of MB with DNA biosensor was measured with differential pulse voltammetry (DPV). The proposed electrochemical DNA biosensor has potential for simple, sensitive and quick determination of carrageenan.
Results and Discussion
Investigation of Immobilized dsDNA Electrochemical Response
Both dsDNA-SPE and bare SPE current signals were measured with DPV after incubated with MB redox mediator, and a significant cathodic peak current (ipc) signal at −0.22 V was observed with dsDNA-SPE compared to the bare SPE (Fig. 3a) as the presence of immobilized dsDNAs possessed plenty of guanine bases for interaction with redox active MB27.
The DPV peak current increased sharply with a positive shifting of the DPV peak potential is typical for the intercalation of MB with dsDNA30. However, a small DPV peak current signal can also be perceived with the bare SPE, which could be due to the non-specific adsorption of MB redox indicator on the SPE surface. The increment in the DPV peak current signal of the dsDNA-SPE also indicates that the dsDNA has been successfully immobilized on the SPE surface.
Figure 3b shows the effect of dsDNA loading on the MB-dsDNA-SPE DPV response at −0.22 V. The biosensor response increased linearly from 5 µg mL−1 to 200 µg mL−1 dsDNA, and the MB ipc signal reduced slightly thereafter until 500 µg mL−1 dsDNA loading. This was attributed to the over loading of dsDNA on the SPE, whereby the high degree of DNA hybridization rate has prevented the interaction between guanine bases and MB, thereby hindered the reduction of MB at the electrode surface.
Accumulation of MB
The amount of MB used to intercalate with the immobilized dsDNA was found to influence the levels of DPV peak current signal. As Fig. 4a indicates, the MB ipc signal increased with the increasing of the MB loading from 10–100 µM due to the increasing amount of guanine bases at the immobilized dsDNA were reacted with the increasing loading of MB.
Besides, the dsDNA-SPE was found to give one-fold greater response than the bare SPE with MB loading because of the available of guanine bases at the dsDNA-SPE for accumulation with the increasing loading of MB. Both electrodes showed saturated response with lower reproducibility performance especially for bare SPE when the MB was loaded above 100 µM. This might be due to the fact that the available guanine nucleotide binding sites at the immobilized dsDNA or the non-specific adsorption sites at the commercial carbon ink-based SPE have fully bound with the excess MB electroactive label.
In general, there are three different basic interaction mechanisms between MB and DNA, which have been identified to be (1) electrostatic interaction of cationic charge in MB with negatively charged phosphate backbone DNA, (2) MB intercalation within dsDNA double-helix (3) and MB binding with the exposed guanine bases on ssDNA31. Among the three ways of MB-DNA interactions, intercalation of MB into dsDNA gives the highest redox peak current signal32. The DPV peak current trending of the DNA-based biosensor following different MB accumulation durations is depicted in Fig. 4b.
The reduction signal of MB increased in the first 5 min as exemplified in the cyclic voltammograms of the dsDNA-SPE (Fig. 4c), which suggests an increasing guanine oxidation reaction rate at the immobilized dsDNA mediated by the MB redox intercalator with time. The intercalation reaction of MB with immobilized dsDNA was then reached to an equilibrium after 5 min of MB accumulation period33,34. Therefore, 5 min MB accumulation duration was optimum for a complete MB intercalation reaction with immobilized dsDNA.
Effects of pH and Ionic Strength
It was found that the DPV peak current signal declined in both acidic and basic electrolyte solutions of Tris-HCl buffer. The maximum MB DPV ipc signal was found with 20 mM Tris-HCl at pH 7.0. At higher or lower pH than pH 7.0, the hydrogen bonds between the nucleobases of DNA double-helix were broken up35, and that the MB intercalation reaction with the dsDNA cannot be occurred as a result of the DNA decomposition36. Therefore, 20 mM Tris-HCl at pH 7.0 was used as the electrolyte solution for all electrochemical measurements. Ionic strength of the electrolyte may affect the intercalation of DNA redox indicator with the dsDNA37. In the present study, low ionic strength electrolyte of 20 mM Tris-HCl buffer (pH 7.0) with 10 mM NaCl was noticed to render a more favourable intercalation of MB with the immobilized dsDNA (Fig. 5).
The addition of positively charged Na+ ion reduced the electrostatic repulsion between the negatively charged sugar-phosphate backbone of the dsDNA, thereby promoted the DNA hybridization reaction rate for subsequent intercalation with the MB redox label34. However, high ionic strength electrolyte solution at 20 mM NaCl and above introduced an ionic shielding to the negatively charged dsDNA sugar-phosphate backbone, which prevented the electrostatic interaction between the cationic MB and dsDNA. This has blocked the MB from reduction at the electrode surface, and that lower DPV peak current signal was obtained.
DNA Biosensor Response towards Carrageenans and Polyanions
The optimized DNA-based biosensor has been tested with different types of carrageenans and polyanions in 20 mM Tris-HCl buffer containing 10 mM NaCl at pH 7.0. Based on the results shown in Fig. 6, the biosensor response declined proportionally with the increasing carrageenan concentration from 1–10 mg L−1.
This was attributed to the increasing released of the cationic MB redox indicator from the immobilized dsDNA for reaction with the respective carrageenans, and this has resulted lesser and lesser amount of MB being retained within the dsDNA-SPE, and that the MB reduction reaction rate at the electrode surface was getting reduced. It was noticed that the DNA-based biosensor demonstrated an overall lower DPV peak current response towards λ-carrageenan compared to ι- and κ-carrageenan due to the highest affinity of the λ-carrageenan for electrostatic binding with the MB based on 1:1 stoichiometry ratio between anionic sites of λ-carrageenan and cationic sites of MB dye molecule21. As the MB molecules released from the MB-dsDNA-SPE, it forms metachromatic complexes with the anionic λ-carrageenans. The dynamic linear concentration range of the DNA the biosensor between 1 mg L−1 and 10 mg L−1 carrageenan gave a regression coefficient (R2) at 0.98 with a limit of detection (LOD) of 0.08 mg L−1. The R-squared coefficient value was obtained from the linear line using format trend line without intercepting at zero coordinate.
κ-carrageenan has the lowest number of ester sulphate functional group, with only one sulphate group per disaccharide, thus the less anionic κ-carrageenan attracted less amounts of MB from the DNA electrode, and left behind a large amounts of immobilized MB within the dsDNA-SPE for mediation of guanine oxidation reaction on the SPE. Negligible response with the MB-dsDNA-SPE was acquired for other polyanions i.e. arabic gum, alginate and starch, whereby relatively constant MB ipc signals were obtained in the biosensor working concentration range. This was because the MB molecules were remained intercalated in dsDNA on the electrode to mediate guanine oxidation response of the immobilized dsDNA as they do not selectively bind with those anionic polysaccharides i.e. starch, arabic gum and alginate. Therefore, it can be deduced that the interaction of the carrageenan with MB is governed by the sulphate content of the carrageenan21,38,39. Interaction between MB and polyanion is electrostatic, reversible, and stoichiometric. It is also interesting to note that the formation of metachromatic complex between carrageenan and MB was immediate without extended incubation duration was required compared to the other polyanions, and this simplified the analysis procedure for carrageenan determination7,21. Furthermore, the biosensor showed reproducible results with relative standard deviation (RSD) attained between 5.6% and 6.9% for three repetitive measurements of carrageenans.
The proposed competitive binding of MB with carrageenan by using the dsDNA electrode could sensitively detect the carrageenan at low levels compared to the previously reported electrochemical potentiometric titration techniques based on immobilized DNNS or TDMAC ionophore in the polymeric membranes with a dynamic linear range reported at 2–20 ppm and detection limit of 0.2 ppm24,25. The new adsorptive transfer constant current derivative chronopotentiometric stripping method for carrageenan detection demonstrated by Strmečki and Plavšić26 was only be able to selectively quantify the κ-carrageenan concentration in the mixture of three carrageenans, and may require the usage of separation technique prior to analyzing the mixture of different types of organic biomolecules. In addition, the detection limit of the chronopotentiometric stripping technique for carrageenan at >6 ppm cannot be applied for the low carrageenan concentration determination purposes.
Determination of Carrageenan in Food Sample
Standard ι-carrageenan has been spiked into the pineapple jelly juice within the calibration concentration range of the biosensor for recovery of carrageenan in the jelly confectionery, and 20 mM Tris-HCl buffer (pH 7.0) was used to stabilize the pH level of the juice. From the results tabulated in Table 1, satisfactory recoveries between 106% and 110% imply that the voltammetric DNA-based biosensor based on competitive reaction of MB with dsDNA and carrageenan can be used for accurate and reliable carrageenan determination in the complex food samples especially in confectioneries.
Comparison of the DNA Biosensor Analytical Performance with Conventional Methods for Carrageenan Determination
Table 2 outlines the analytical performance of the developed electrochemical DNA biosensor and some other official methods commonly used for the assay of carrageenan in terms of detection limit, response time, simplicity and chemical consumption.
Gas chromatography and high-performance anion-exchange chromatography techniques which are sensitive for the determination of polysaccharides, however, they require elaborate hydrolysis step using acid e.g. trifluoroacetic acid in order to digest the polysaccharides into monosaccharides before determination of carrageenan can be done with the chromatographic methods. Furthermore, they involve long analysis time of close to an hour with high chemical consumption, and must be operated by trained personnel.
Evaluation of carrageenan with spectrofluorimetric and colorimetric methods, on the other hand, would require gravimetric precipitation process to be carried out followed by mixing with chemical absorption solvent such as monoethanolamine prior to optical measurement. This makes direct detection of carrageenan become impossible as aqueous amine solution is technically required following the precipitation step. As such, the proposed electrochemical DNA biosensor could provide promise to improve the analytical performance for quick, sensitive and convenient application with regard to direct determination of carrageenan without any pre-treatment of sample and derivatization.
Methods
Apparatus
DPV and cyclic voltammetry (CV) measurements were carried out by using AUTOLAB PGSTAT 12 potentiostat/galvanostat controlled by GPES 4.9 software (Eco Chemie, The Netherlands). The three-electrode system consists of a carbon SPE working electrode, which was purchased from Scrint Technology (M) Sdn Bhd, a Metrohm Ag/AgCl (3 M KCl) reference electrode and a glassy carbon counter electrode.
Reagents and Materials
dsDNA stock solution was prepared by dissolving 5 mg of double-stranded calf thymus DNA powder (activated and lyophilized powder, catalog number 4522, Sigma) in TE buffer, which composed of 10 mM Tris-HCl (Acros Organics) and 1 mM EDTA (Duchefa Biochemie) at pH 8.0, and kept at 4 °C. Stock solution of methylene blue (MB, R & M Chemicals) at 1 mM was prepared in deionized water and stored at 4 °C in a dark environment. Analytical grade of ι-(iota) and λ-(lambda) carrageenans from Sigma, and κ-(kappa) carrageenan, calcium alginate, starch and gum arabic from Fluka were prepared in deionized water at 60 °C in a water bath followed by cooling to room temperature (25 °C), and diluted with deionized water to a final concentration of 100 µg mL−1. Dissolution of dsDNA, MB and carrageenan stock solutions were performed by using 20 mM Tris-HCl buffer and 10 mM NaCl (Sigma) at pH 7.0. Other chemicals used were of analytical reagent grade and all the solutions were prepared in Milli-Q deionized water (18 MOhm).
Carrageenan Biosensor Fabrication
DNA immobilization was performed by depositing 3 μL of calf thymus dsDNA stock solution on the SPE and left to dry overnight at ambient conditions. The resulting dsDNA-modified SPE (dsDNA-SPE) was immersed in 20 mM Tris-HCl buffer for 5 min to remove the unbound dsDNA on the SPE electrode40,41. The dsDNA-SPE was then soaked in MB solution at open-circuit potential for 7 min, and rinsed again with copious amounts of 20 mM Tris-HCl buffer for another 10 s to remove those loosely bound MB from the DNA electrode. After that, the dsDNA-SPE modified with MB (MB-dsDNA-SPE) was immersed in the carrageenan solution for 2 min followed by rinsing with abundant amounts of 20 mM Tris-HCl buffer for 5 s before electrochemical scanning. Electrochemical measurement was carried out in 20 mM Tris-HCl buffer containing 10 mM NaCl at pH 7.0 between −0.6 and 0 V versus Ag/AgCl electrode with a scan rate at 0.005 V s-1 and step potential of 0.01 V.
Optimization of Carrageenan DNA Biosensor
Several parameters were optimized before the DNA-based biosensor was used for carrageenan determination in edible jelly food sample. The amount of dsDNA loaded on the SPE was varied between 5 µg mL−1 and 200 µg mL−1 in order to obtain the highest DPV peak current response at −0.22 V in a mixed 20 mM Tris-HCl/10 mM NaCl buffer at pH 7.0. Optimization of MB loading on the dsDNA-SPE was carried out by varying the MB amount from 10–180 µM, whilst MB accumulation duration between 1 min and 9 min was investigated to achieve optimum biosensor performance using a constant dsDNA loading at 200 µg mL−1. The effect of electrolyte pH towards MB ipc signal of the MB-dsDNA-SPE was examined by using a mixed 20 mM Tris-HCl/10 mM NaCl electrolyte solution between pH 2.0 and pH 11.0 with MB accumulation time was maintained at 5 min. Sodium ion (Na+) in the concentration range of 10–80 mM was used to adjust the ionic strength of 20 mM Tris-HCl electrolyte solution at pH 7.0. The DNA-based biosensor response against different types of carrageenans such as λ-, ι- and κ-carrageenans, and various polyanions including gum arabic, alginate and starch were studied by immersing the MB-dsDNA-SPE into the respective carrageenans or polyanions in 20 mM Tris-HCl buffer containing 10 mM NaCl at pH 7.0 for 2 min prior to DPV measurement at −0.22 V. Finally, recovery study was carried out by using pineapple jelly purchased from local hypermarket. The jelly was cut into pieces followed by blending 200 g of the jelly in 400 mL of deionized water. The pineapple pulp was then filtered away to obtain the clear juice and diluted with 20 mM Tris-HCl buffer (pH 7.0) to a final volume of 500 mL. Subsequently, about 250 mL of the juice was used to dissolve 0.3 mg of ι-carrageenan, and heated in a water bath at 60 °C for 15 min before left to cool to room temperature and stored in a refrigerator (4 °C).
Conclusions
Biosensor determination of carrageenan by using DPV technique has been shown to be a simpler, faster and more sensitive method compared to the other previously reported chromatographic and spectrophotometric methods. The DNA-based biosensor is selective towards carrageenan detection via competitive electrostatic interaction of dsDNA and carrageenan towards MB redox label in the solution. DNA immobilization based on dry-adsorption method on the disposable SPE surface under open-circuit without any electrochemical pre-conditioning allows mass production of this electrochemical carrageenan biosensor in a short period of time at the lowest cost.
Data Availability
All data are fully available without restriction.
References
Campo, V. L., Kawano, D. F., Silva, D. B. D. Jr. & Carvalho, I. Carrageenans: Biological properties, chemical modifications and structural analysis–a review. Carbohydr Polym. 77, 167–180 (2009).
Alistair, S. & Shirley, C. Introduction in Food polysaccharides and their applications 1–24 (Chemical Rubber Company Press, 2006).
Loh, K. S., Liew, J. W. Y., Azizan, A., Lim, K. L. & Wan Ramli, W. D. Synthesis and characterization of modified κ-carrageenan for enhanced proton conductivity as polymer electrolyte membrane. Plos One. 12, e0185313 (2017).
Nasima, A. et al. Investigation of the gelation mechanism between amino acid surfactant based microemulsion and kappa-carrageenan gel network. Sains Malays. 43, 203–209 (2014).
Jiao G., Yu G., Zhang J. & Ewart H.S. Chemical structures and bioactivities of sulfated polysaccharides from marine algae. Mar Drugs. 196–223 (2011).
Nor Syamimi C. J., Syazwan A. & Saiful I. Z. Efficacy study of carrageenan as an alternative infused material (filler) in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) porous 3D scaffold. Int J Polym Sci. 1–12 (2017).
Roberts, M. A. & Quemener, B. Measurement of carrageenans in food: Challenges, progress, and trends in analysis. Trends Food Scie Technol. 10, 169–181 (1999).
Steve, C. & Yolanda, B. Food polysaccharides and their applications 675–712 (Chemical Rubber Company Press, 2006).
Shamsudin, I. J., Ahmad, A., Hassan, N. H. & Kaddami, H. Solid biopolymer electrolyte based on κ-carrageenan for electrochemical devices application. Asian J Chem. 26, 77–80 (2014).
Mobaraka, N. N., Ramlia, N., Ahmad, A. & Rahman, M. Y. A. Chemical interaction and conductivity of carboxymethyl κ-carrageenan based green polymer electrolyte. Solid State Ion. 224, 51–57 (2012).
Al-Hazmi, M. I. & Stauffer, K. R. Gas chromatographic determination of hydrolyzed sugars in commercial gums. J Food Sci. 51, 1091–1092 (1986).
Lawrence, J. F. & Iyengar, J. R. Gas chromatographic determination of polysaccharide gums in foods after hydrolysis and derivatization. J Chromatogr A. 350, 237–244 (1985).
Quemener, B., Lahaye, M. & Metro, F. Assessment of methanolysis for the determination of composite sugars of gelling carrageenans and agarose by HPLC. Carbohydr Res. 266, 53–64 (1995).
Uno, Y. et al. Molecular weight distribution of carrageenans studied by a combined gel permeation/inductively coupled plasma (gpc/icp) method. Food addit contam. 18, 763–772 (2001).
Jol, C. N., Neiss, T. G., Penninkhof, B., Rudolph, B. & De Ruiter, G. A. A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose. Anal Biochem. 268, 213–222 (1999).
Lamari, F. N., Kuhn, R. & Karamanos, N. K. Derivatization of carbohydrates for chromatographic, electrophoretic and mass spectrometric structure analysis. J Chromatogr B. 793, 15–36 (2003).
Graham, H. D. Quantitative determination of carrageenan in milk and milk products using papain and cetyl pyridinium chloride. J Food Sci. 33, 390–394 (1968).
Shirai, M., Nagaoka, Y. & Tanaka, M. Interaction between dyes and polyelectrolytes. Iv. Metachromatic behavior of methylene blue induced by poly(vinylphenol). J Polym Sci: Polym Chem. 15, 1021–1025 (1977).
Éwe, G. E. Methylene blue U. S. P. as precipitant of irish moss. J Am Pharm Assoc. 19, 568–570 (1930).
Graham, H. D. Quantitative aspects of the interaction of carrageenan with cationic substances.I. Interaction with methylene blue. J Food Sci. 25, 720–730 (1960).
Soedjak, H. S. Colorimetric determination of carrageenans and other anionic hydrocolloids with methylene blue. Anal Chem. 66, 4514–4518 (1994).
Yabe, Y., Ninomiya, T., Tatsuno, T. & OKAD, A. Simple colorimetric determination of carrageenan in jellies and salad dressings. J Assoc Off Anal Chem. 74, 1019–1022 (1991).
Cundall, R., Phillips, G. & Rowlands, D. A spectrofluorimetric procedure for the assay of carrageenan. Analyst. 98, 857–862 (1973).
Hassan, S. S., Meyerhoff, M., Badr, I. & Abd‐Rabboh, H. Determination of carrageenan in food products using potentiometric polyion sensors. Electroanalysis. 14, 439–444 (2002).
DÜRÜST, N. & Kayansalan, A. Potentiometric determination of some food additives and their binding to a polycationic species using polyion sensors. Turk J Chem. 37, 308–315 (2013).
Strmečki, S. & Plavšić, M. An ex situ electrocatalytic analysis of κ-, ι-, and λ-carrageenan on mercury electrode in seawater. J Electroanal Chem. 712, 1–7 (2014).
Wang, J. et al. Indicator-free electrochemical DNA hybridization biosensor. Anal Chim Acta. 375, 197–203 (1998).
Lucarelli, F., Palchetti, I., Marrazza, G. & Mascini, M. Electrochemical DNA biosensor as a screening tool for the detection of toxicants in water and wastewater samples. Talanta. 56, 949–957 (2002).
Pan, D. et al. Electrochemical interrogation of interactions between surface-confined DNA and methylene blue. Sensors. 7, 2671–2680 (2007).
Zhu, L. et al. Electrochemical behaviors of methylene blue on DNA modified electrode and its application to the detection of pcr product from nos sequence. Sensors. 8, 5649–5660 (2008).
Nasef, H., Beni, V. & O’Sullivan, C. K. Methylene blue as an electrochemical indicator for df508 cystic fibrosis mutation detection. Anal bioanal chem. 396, 1423–1432 (2010).
Kara, P. et al. Electrochemical genosensor for the detection of interaction between methylene blue and DNA. Electrochem commun. 4, 705–709 (2002).
Lin, X. H., Wu, P., Chen, W., Zhang, Y. F. & Xia, X. H. Electrochemical DNA biosensor for the detection of short DNA species of chronic myelogenous leukemia by using methylene blue. Talanta. 72, 468–471 (2007).
Loaiza, Ó. A., Campuzano, S., López-Berlanga, M., Pedrero, M. & Pingarrón, J. M. Development of a DNA sensor based on alkanethiol self-assembled monolayer-modified electrodes. Sensors. 5, 344–363 (2005).
Zhai, J., Cui, H. & Yang, R. DNA based biosensors. Biotechnol Adv. 15, 43–58 (1997).
Tremblay, S., Douki, T., Cadet, J. & Wagner, J. R. 2′-deoxycytidine glycols, a missing link in the free radical-mediated oxidation of DNA. J Biol Chem. 274, 20833–20838 (1999).
Arias, P., Ferreyra, N. F., Rivas, G. A. & Bollo, S. Glassy carbon electrodes modified with cnt dispersed in chitosan: Analytical applications for sensing DNA–methylene blue interaction. J Electroanal Chem. 634, 123–126 (2009).
Michon, C., Konaté, K., Cuvelier, G. & Launay, B. Gelatin/carrageenan interactions in coil and ordered conformations followed by a methylene blue spectrophotometric method. Food Hydrocoll. 16, 613–618 (2002).
Williams, P. A. et al. Effect of gelatin/carrageenan interactions on the structure and the rheological properties of the mixed system in Gums and stabilisers for the food industry 177-184 (Royal Society of Chemistry, 2002).
Bučková, M. et al. Detection of damage to DNA and antioxidative activity of yeast polysaccharides at the DNA-modified screen-printed electrode. Talanta. 56, 939–947 (2002).
Labuda, J. et al. Detection of antioxidative activity of plant extracts at the DNA-modified screen-printed electrode. Sensors. 2, 1–10 (2002).
Acknowledgements
We acknowledge financial support from the Faculty Science & Technology, Universiti Kebangsaan Malaysia, Ministry of Eduction, Malaysia via grant FRGS/1/2012/ST01/UKM/01/1 and Department of Fishery Malaysia and Institut Penyelidikan Perikanan Malaysia via grant STGL-007-2010/10.
Author information
Authors and Affiliations
Contributions
R.A.H. performed the laboratory works, R.A.H. and T.L.L. wrote the manuscript and L.Y.H. supervised the study. All authors reviewed the manuscript.
Corresponding authors
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Hassan, R.A., Heng, L.Y. & Tan, L.L. Novel DNA Biosensor for Direct Determination of Carrageenan. Sci Rep 9, 6379 (2019). https://doi.org/10.1038/s41598-019-42757-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-42757-y
This article is cited by
-
On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium
Scientific Reports (2022)
-
Longer amplicons provide better sensitivity for electrochemical sensing of viral nucleic acid in water samples using PCB electrodes
Scientific Reports (2022)
-
Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA
Scientific Reports (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.