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# A vector holographic optical trap

## Abstract

The invention of optical tweezers almost forty years ago has triggered applications spanning multiple disciplines and has also found its way into commercial products. A major breakthrough came with the invention of holographic optical tweezers (HOTs), allowing simultaneous manipulation of many particles, traditionally done with arrays of scalar beams. Here we demonstrate a vector HOT with arrays of digitally controlled Higher-Order Poincaré Sphere (HOPS) beams. We employ a simple set-up using a spatial light modulator and show that each beam in the array can be manipulated independently and set to an arbitrary HOPS state, including replicating traditional scalar beam HOTs. We demonstrate trapping and tweezing with customized arrays of HOPS beams comprising scalar orbital angular momentum and cylindrical vector beams, including radially and azimuthally polarized beams simultaneously in the same trap. Our approach is general enough to be easily extended to arbitrary vector beams, could be implemented with fast refresh rates and will be of interest to the structured light and optical manipulation communities alike.

## Introduction

Since their inception in 19701, optical tweezers have become a versatile non-invasive technique to manipulate micro and nano structures. Key to this is the notion that light carries momenta: linear momentum (known at least as far back as Kepler), spin angular momentum2,3 and orbital angular momentum4, with a change in momentum resulting in a force. In physics for example, the interaction of such optical forces with atomic ensembles paved the way towards the invention of powerful techniques for laser cooling5,6,7, which in turn allowed the realization of the Bose-Einstein condensate8,9. In biology, it provided with a perfect non-invasive tool for the study of single molecules and cells, for example, to facilitate the understanding of all the complex dynamic processes occurring inside a cell or to analyze the mechanical properties of DNA10,11,12,13,14,15. Extensive reviews of all the applications that optical tweezers have facilitated can be found in refs16,17,18,19,20 and references therein.

A major breakthrough in optical manipulation techniques came from the field of structured light21, especially the use of the Spatial Light Modulators (SLMs)22,23 to produce holographic optical traps (HOTs)24,25,26. SLMs are computer controlled devices based on liquid crystal technology that allow, amongst other things, a fast and precise generation and control of almost any beam shape, their digital focusing and propagation, as well as the simultaneous generation and detection of high numbers of individual beams27,28 which have been used to control multiple particles in 2D and 3D configurations29,30,31,32,33 as well as along exotic trajectories34,35,36,37,38,39,40,41,42.

The aforementioned studies were done with scalar light, while recently it has become topical to explore vector states of light43,44. Along this line, the generation methods have evolved from simple interferometric techniques, capable of generating only one vector beam, to more complicated configurations relying on spatial light modulators43,45,46,47,48. There is interest in using such beams in optical traps due to the enhanced focusing ability of certain vector states of light49,50,51,52,53,54,55,56,57 thereby improving the trapping efficiency49,58. For example, Michihata et al. pointed out that radially polarized beams enhance the axial trapping efficiency near 2× compared to linearly polarized beams, at the expense of reducing the transverse efficiency by about a half49. Other studies have shown that radially and azimuthally polarized beams exhibit higher axial trapping forces on core-shell magnetic microparticles as compared to Gaussian beams50. Direct comparison of azimuthally and radially polarized vector beams has shown that the former features a stronger lateral trapping force compared to the latter51,52. These topical beams are described by states on a generalized Poincaré Sphere59,60, the so-called Higher-Order Poincaré Sphere (HOPS), to account for the total angular momentum of light, spin and angular59.

Here we introduce a HOT for arbitrary HOPS beams that allows for multiple HOPS beams to be delivered into a trap in some desired array. Each HOPS beam in the array is independently controlled by an SLM and may be switched from scalar states (on the poles of the HOPS) to cylindrical vector vortex beams (on the equator of the HOPS). By this approach, we are able to tailor on-demand the 3D shape of the optical forces at specific locations inside the optical trap. To show the potential of this technique, we simultaneously compare the trapping strength of cylindrical vector vortex and scalar vortex beams in the same trap. While our vector HOT technique is implemented here with an SLM and delivers arrays of HOPS beams, we emphasize that this may easily be extended to faster refresh rates with digital micro-mirror devices (DMDs)61 as well as to arbitrary vector states beyond HOPS beams. Our work introduces a new HOT tool which we believe will be beneficial to structured light and optical manipulation studies alike.

## Generating arrays of Poincaré beams

Vector beams are commonly generated as coaxial superpositions of orthogonal scalar fields with orthogonal polarization states. Mathematically, they can be represented as

$$U(r)={U}_{R}\hat{{\bf{R}}}+{U}_{L}\hat{{\bf{L}}},$$
(1)

where $$\hat{{\bf{R}}}$$ and $$\hat{{\bf{L}}}$$ represent the unitary vectors of the right and left circular polarization, respectively, and UR and UL are orthogonal scalar fields. Using the Laguerre Gaussian basis as an example we have

$$U(r)=L{G}_{{p}_{1}}^{{\ell }_{1}}{{\rm{e}}}^{i{\alpha }_{1}}\hat{{\bf{R}}}+L{G}_{{p}_{2}}^{{\ell }_{2}}{{\rm{e}}}^{-i{\alpha }_{2}}\hat{{\bf{L}}}.$$
(2)

here $$L{G}_{{p}_{1}}^{{\ell }_{1}}$$ and $$L{G}_{{p}_{2}}^{{\ell }_{2}}$$ are the amplitudes of each orthogonal component and represent the well-known Laguerre-Gaussian modes with azimuthal and radial indexes ℓ and p, respectively, while α1 and α2 define modal phases. In this study, we will restrict ourselves to modes with ℓ = −ℓ2 = ±ℓ, α1 = 0 and α2 = α in order to create HOPS beams

$$U(r,{\rm{\Phi }},{\rm{\Theta }})=\,\cos (\frac{{\rm{\Phi }}}{2})L{G}_{0}^{\ell }{{\rm{e}}}^{-i{\rm{\Theta }}\mathrm{/2}}\hat{{\bf{R}}}+\,\sin (\frac{{\rm{\Phi }}}{2})L{G}_{0}^{\ell }{{\rm{e}}}^{i{\rm{\Theta }}\mathrm{/2}}\hat{{\bf{L}}},$$
(3)

where Φ  [0, π] and Θ  [0, 2π] are the coordinates on the HOPS, as shown in Fig. 1(a,b). In this geometric representation, the left and right circularly polarized orbital angular momentum (OAM) states are located on the poles (our scalar vortex beams); the cylindrical vector vortex beams lie along the equator; and intermediate states occupy the rest of the sphere. Such beams have been created by a myriad of techniques47,62 and found many applications44,63,64.

To generate a single vector beam, we first generate two scalar beams by multiplexing their corresponding holograms onto a SLM, each encoded with a unique spatial frequency to ensure their propagation along different paths. This way we can manipulate the polarization on each path independently. To realize HOPS beams is necessary to superimpose appropriate scalar beams with orthogonal polarizations. Since the SLM only modulates one polarization, a HWP was introduced in one path to rotate their polarization. A PBS was used to achieve co-linear recombination followed by a quarter wave plate to create the desired mode. The beauty of this approach lies in the fact that all properties of each individual beam can be manipulated digitally, without any mechanical component, allowing the generation of any beam on the HOPS. For example, to generate all cases shown in Fig. 1(a), labeled as 1, 2, 3, and 4, one needs only to adjust the relative phase and amplitude of each scalar beam, associated to the parameters Θ and Φ, respectively. For the case of the radially polarized beam, labeled as 1, the corresponding parameters are: ℓ = 1, Φ = π/2 and Θ = 0 (see Fig. 1(c,i) inset 1). By superimposing each of the multiplexed pairs of holograms into a single hologram, as shown in Fig. 1(c,ii), the simultaneous generation of multiple vector beams can be realized.

## Results

### Arrays of HOPS beams

Using our approach, we created a square array of 3 × 3 HOPS beams with topological charges ℓ = 1 and ℓ = 2 for 9 arbitrarily chosen polarization structures, and delivered these through the system. The intensity of the experimentally generated array of HOPS beams is shown in Fig. 2(a). Intensity differences between vector beams in the bottom row in comparison to those at above rows arise from the distinct deflection angles out of the SLM, such that the more intense are less deflected from the undiffracted zero order. Importantly, by adjusting the phase modulation depth on the encoded hologram, equal intensity distributions can be easily attained. All 9 beams were delivered to the desired locations (xi, yi) in the trapping plane, here designed to be in a square array. Table 1 contains the values of the beam positions at the trapping plane as observed on the image, their corresponding grating frequencies (xu, yu) and (xv, yv) for paths U and V, respectively, and the calculated angles relative to the undiffracted zero order beam as determined according to ref.46 These values clearly show that by modifying grating frequencies in the hologram, we can chose the arrangement of the vector beams at will. To determine the quality of each HOPS beam, we performed a spatially resolved stokes measurement43,65. Figure 2(b–d) shows the measured polarization distributions of beams 1, 2 and 3 of the 9 multiplexed vector beams. The inserts show the corresponding theoretically generated intensity and polarization distributions, which are in good agreement with the experimental results confirming the creation of vector beams. Because the beams are created in a 2D array, the measurements could be performed simultaneously on all 9 beams.

### 2D Optical traps with arrays of HOPS beams

In previous sections we described how to create arrays of reconfigurable HOPS beams; now we wish to employ this in a vector HOT. To this end, we projected an array of created vector beams into the entrance pupil of the objective with each HOPS beam at an appropriate incident angle so as to produce the desired 2D array of traps. By way of example, we trapped beads in a triangular array, illustrated in Fig. 3(a) and shown experimentally in (b). Figure 3(b) shows six particles of diameter ≈2 μm, trapped with ≈10 mW of average power arranged in a triangular configuration. Since our approach allows the independent creation of any arbitrary polarization state on the HOPS, we demonstrated multiple optical traps with six arbitrary positions on the HOPS, as shown in Fig. 3(c). Here we also make clear that the vector HOT can also reproduce traditional scalar HOTs: two of the six beams in the triangular array are in fact scalar OAM beams from the poles of the HOPS. All beam insets in Fig. 3(b) are experimentally measured intensity profiles, and insets in (c) show the intensity after a linear polarizer, as explained in the caption.

### Optical forces in our vector HOT

Finally, as a proof-of-concept application of our technique, we trap three beads simultaneously in a linear array and performed a drag force test. We create HOPS beams that are radially, azimuthally and linearly polarized at the plane of the trap, as shown in Fig. 4. Such a configuration allows for the direct comparison of the trap stiffness for each beam. First, we corroborated that the trapping strength of each beam is position independent. For this, we interchange beam 1 (azimuthal) with beams 2 (linearly) and 3 (azimuthal). That is, from position x to position y and z. We find that the outcome was independent of position. Radially polarized beams exert the weakest trapping force, as compared to azimuthally and linearly polarized beams which remain trapped for higher speeds of ~55 μm/s and 64 μm/s, respectively. Some frames of this test are shown in Fig. 4 and the associated media file.

We measured the lateral trapping strength of each beam, with the results shown in Table 2. From the results we observe that the linearly polarized scalar beam has the strongest transverse trapping force, with the radially polarized beam the least. These results are in agreement with previous reports49,51,52.

## Discussion

The approach we have outlined allows arbitrary arrays of vector beams to be created and delivered to a trap, resulting in the demonstration of a vector HOT with multiple beams. Importantly, as we have shown, the vector HOT can be reduced to a traditional scalar HOT by simply switching one hologram off, and is versatile enough to allow both scalar and vector beams to be used in the same trap. As with traditional HOT, the hologram efficiencies must be adjusted to ensure the power delivered to each trap is as desired. In our experiments, we paid particular attention to this fact, particularly in the trapping stiffness tests, to ensure that only the polarization structures differed and that each beam has the same power at the trap. Related to this is the importance of due care with choice of optics in the delivery system in order to maintain the desired polarization structure of the beams in the vector HOT.

In this work, we have demonstrated the vector HOT using SLMs, HOPS beams and 2D arrays of traps. The SLM may be replaced with equivalent technology for faster refresh rates, e.g., digital micro-mirror devices (DMDs). Similarly, it should be clear from Eq. 1 that HOPS are an example only and that any arbitrary vector beam can be created at each location in the HOT array. Moreover, by switching off one component of the vector field at the SLM, the well-known scalar holographic trap can be reproduced, and as we have shown, vector and scalar beams may be produced at the same time in the same trap array. Given the many applications of scalar holographic traps, it is reasonable to assume that this new vector holographic trap too will find many applications, for example, in the trapping of gold nano-particles for plasmonic studies where vector beams have known benefits, inducing optical chirality in form birefringent structures, exploiting known vector trapping efficiency to adjust trapping strengths, e.g., in biological matter control with the same modal conditions (power and intensity profile) but differing polarizations for differing trap stengths, to execute fibre traps with vector modes, the natural modes of most fibres, and even in fundamental studies of light-matter interaction to compare phase singularities with polarization singularities (scalar OAM being an example of a phase singularity with the vector HOPS having a polarization singularity). Finally, by following the advances of scalar HOT, it will be possible to adjust the trapping plane of each vector beam by adding individual digital lenses to each beam23 for the realization of 3D traps66. We believe that since our system can produce both vector and scalar beams holographically at the same time, the addition of the polarization degree of freedom through vector beams will open new applications to follow.

To summarize, we have introduced and demonstrated the concept of a vector HOT for the delivery of 2D arrays of traps using HOPS beams. We created example arrays (square, triangular and linear) with combinations of scalar and vector HOPS beams, characterized the beams externally to the trap, and then demonstrated successful trapping with them. Each HOPS beam in the array was independently controlled by a SLM and spanned a wide range of positions on HOPS, including those associated with conventional scalar HOTs. By this approach, we were able to tailor on-demand the 3D shape of the optical forces at specific locations inside the optical trap, enabling the simultaneous realization of many traps with different trapping strength for the same laser power. We have pointed out how our vector HOT technique may be further advanced, which we hope will stimulate interest from the structured light and optical manipulation communities alike.

## Methods

### A vector HOT with multiple HOPS beams

A schematic representation of the experimental setup used to generate arrays of optical traps with multiple vector beams is illustrated in Fig. 5. In our implementation, we used a horizontally polarized laser beam (λ = 532 nm, Coherent Verdi G) incident on a reflective SLM (Holoeye Pluto) aligned to modulate horizontal polarization for multiple vector beams generation. The inclusion of a telescope (lenses L1 and L2 in the figure; f1 = 500 mm, f2 = 1000 mm) is a crucial step required to send the HOPS beam into the optical trap. On one hand, by placing the lenses following a 4 f configuration, it images the SLM plane at the front focal plane of the microscope objective (100× Nikon oil immersion, N100X-PFO, NA 1.3). By doing this, unique propagation angles (as programmed on the SLM) will translate into unique (xi, yi) positions in the trapping plane: beams from the SLM propagate at differing angles which are mapped to positions with L1. The set-up converges the light at the PBS for interferometric recombination to produce the desired vector beams. To recreate the position control in the trap, L2 maps position to angle such that each vector beam incident at the objective will be focused to a unique position. On the other hand, focal length of the lenses of the telescope where chosen to ensure a 2x magnification to expand the beams and fill the entrance pupil of the microscope objective, ensuring tight focusing, then providing the necessary intensity gradient for trapping. A half wave plate (HWP) was introduced along path V to rotate the polarization from horizontal to vertical such that coaxial superposition of orthogonal polarization states was attained. The introduction of a HWP results in an optical path difference and potentially undesired phase delays which may cause a rotation in the polarization states of the generated vector beams. Since our created vector beams were cylindrical symmetric, the rotational effect cannot be observed. Nevertheless, to keep the system general, the optical path difference was corrected for by performing translation adjustments of the mirror in path U, countering the unwanted phase shift. The objective focused the beams into a sample container of depth 400 μm holding 2 μm diameter silica beads diluted in deionized water. An inverted microscope setup with an illuminating white light-emitting diode and a CCD camera was used to image the trapped beads. A dichroic mirror (DM) was used to reflect the vector beams into the objective while allowing white light from the light emitting diode to pass through to the CCD camera.

## Data Availability Statement

All data regarding the work presented here is available upon reasonable request to the corresponding author.

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## References

1. 1.

Ashkin, A. Acceleration and trapping of particles by radiation pressure. Phys. Rev. Lett. 24, 156–159 (1970).

2. 2.

Poynting, J. H. On the transfer of energy in the electromagnetic field. Phil. Trans. R. Soc. Lond. 175, 343–361 (1884).

3. 3.

Beth, R. A. Mechanical detection and measurement of the angular momentum of light. Phys. Rev. 50, 115–125 (1936).

4. 4.

Allen, L., Beijersbergen, M. W., Spreeuw, R. J. C. & Woerdman, J. P. Orbital angular momentum of light and the transformation of laguerre-gaussian laser modes. Phys. Rev. A 45, 8185–8189 (1992).

5. 5.

Chu, S. The manipulation of neutral particles. Rev. Mod. Phys. 70, 685–706 (1998).

6. 6.

Phillips, W. D. Laser cooling and trapping of neutral atoms. Rev. Mod. Phys. 70, 721–740 (1998).

7. 7.

Cohen-Tannoudji, C. Manipulating atoms with photons. Rev. Mod. Phys. 70, 707–719 (1998).

8. 8.

Anderson, M. H., Ensher, J. R., Matthews, M. R., Wieman, C. E. & Cornell, E. A. Observation of bose-einstein condensation in a dilute atomic vapor. Sci. 269, 198–201 (1995).

9. 9.

Davis, K. B. et al. Bose-einstein condensation in a gas of sodium atoms. Phys. Rev. Lett. 75, 3969–3973 (1995).

10. 10.

Ashkin, A., Dziedzic, J. M. & Yamane, T. Optical trapping and manipulation of single cells using infrared laser beams. Nat. 330, 769 (1987).

11. 11.

Ashkin, A. & Dziedzic, J. M. Internal cell manipulation using infrared laser traps. Proc. Natl. Acad. Sci. United States Am. 86, 7914–7918 (1989).

12. 12.

Perkins, T., Quake, S., Smith, D. & Chu, S. Relaxation of a single dna molecule observed by optical microscopy. Sci. 264, 822–826 (1994).

13. 13.

Zhang, H. & Liu, K.-K. Optical tweezers for single cells. J. Royal Soc. Interface 5, 671–690 (2008).

14. 14.

Neuman, K. C. & Nagy, A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat. Methods 5, 491 (2008).

15. 15.

Favre-Bulle, I. A., Stilgoe, A. B., Rubinsztein-Dunlop, H. & Scott, E. K. Optical trapping of otoliths drives vestibular behaviours in larval zebrafish. Nat. communications 8, 630 (2017).

16. 16.

Molloy, J. E. & Padgett, M. J. Lights, action: Optical tweezers. Contemp. Phys. 43, 241–258 (2002).

17. 17.

Neuman, K. C. & Block, S. M. Optical trapping. Rev. Sci. Instruments 75, 2787–2809 (2004).

18. 18.

Moffitt, J. R., Chemla, Y. R., Smith, S. B. & Bustamante, C. Recent advances in optical tweezers. Annu. Rev. Biochem. 77, 205–228 (2008).

19. 19.

Maragò, O. M., Jones, P. H., Gucciardi, P. G., Volpe, G. & Ferrari, A. C. Optical trapping and manipulation of nanostructures. Nat. Nanotechnol. 8, 807 (2013).

20. 20.

Daly, M., Sergides, M. & Chormaic, S. N. Optical trapping and manipulation of micrometer and submicrometer particles. Laser & Photonics Rev. 9, 309–329 (2015).

21. 21.

Rubinsztein-Dunlop, H. et al. Roadmap on structured light. J. Opt. 19, 013001 (2017).

22. 22.

Forbes, A., Dudley, A. & McLaren, M. Creation and detection of optical modes with spatial light modulators. Adv. Opt. Photonics 8, 200–227 (2016).

23. 23.

Rosales-Guzmán, C. & Forbes, A. How to shape light with spatial light modulators (SPIE Press, 2017).

24. 24.

Dholakia, K., MacDonald, M. & Spalding, G. Optical tweezers: the next generation. Phys. World 15, 31 (2002).

25. 25.

Grier, D. G. A revolution in optical manipulation. Nat. 424, 810 (2003).

26. 26.

Dholakia, K. & Lee, W. Optical trapping takes shape: The use of structured light fields. In Advances in Atomic, Molecular, and Optical Physics, 56, 261–337 (Academic Press, 2008).

27. 27.

Rosales-Guzmán, C., Bhebhe, N., Mahonisi, N. & Forbes, A. Multiplexing 200 spatial modes with a single hologram. J. Opt. 19, 113501 (2017).

28. 28.

Trichili, A. et al. Optical communication beyond orbital angular momentum. Sci. Rep. 6, 27674 (2016).

29. 29.

Preece, D. et al. Independent polarisation control of multiple optical traps. Opt. express 16, 15897–15902 (2008).

30. 30.

Dufresne, E. R., Spalding, G. C., Dearing, M. T., Sheets, S. A. & Grier, D. G. Computer-generated holographic optical tweezer arrays. Rev. Sci. Instruments 72, 1810–1816 (2001).

31. 31.

Sinclair, G. et al. Assembly of 3-dimensional structures using programmable holographic optical tweezers. Opt. Express 12, 5475–5480 (2004).

32. 32.

Curtis, J. E., Koss, B. A. & Grier, D. G. Dynamic holographic optical tweezers. Opt. Commun. 207, 169–175 (2002).

33. 33.

Padgett, M. & Di Leonardo, R. Holographic optical tweezers and their relevance to lab on chip devices. Lab Chip 11, 1196–1205 (2011).

34. 34.

He, H., Friese, M. E. J., Heckenberg, N. R. & Rubinsztein-Dunlop, H. Direct observation of transfer of angular momentum to absorptive particles from a laser beam with a phase singularity. Phys. Rev. Lett. 75, 826–829 (1995).

35. 35.

Padgett, M. & Allen, L. Optical tweezers and spanners. Phys. World 10, 35 (1997).

36. 36.

Friese, M. E. J., Nieminen, T. A., Heckenberg, N. R. & Rubinsztein-Dunlop, H. Optical alignment and spinning of laser-trapped microscopic particles. Nat. 394, 348 (1998).

37. 37.

Reicherter, M., Haist, T., Wagemann, E. U. & Tiziani, H. J. Optical particle trapping with computer-generated holograms written on a liquid-crystal display. Opt. Lett. 24, 608–610 (1999).

38. 38.

Padgett, M. J. & Allen, L. The angular momentum of light: optical spanners and the rotational frequency shift. Opt. Quantum Electron. 31, 1–12 (1999).

39. 39.

Paterson, L. et al. Controlled rotation of optically trapped microscopic particles. Sci. 292, 912–914 (2001).

40. 40.

Padgett, M. & Bowman, R. Tweezers with a twist. Nat. Photonics 5, 343 (2011).

41. 41.

Baumgartl, J., Mazilu, M. & Dholakia, K. Optically mediated particle clearing using airy wavepackets. Nat. Photonics 2, 675 (2008).

42. 42.

Daria, V. R., Palima, D. Z. & Glückstad, J. Optical twists in phase and amplitude. Opt. Express 19, 476–481 (2011).

43. 43.

Zhan, Q. Cylindrical vector beams: from mathematical concepts to applications. Adv. Opt. Photonics 1, 1–57 (2009).

44. 44.

Ndagano, B., Nape, I., Cox, M. A., Rosales-Guzman, C. & Forbes, A. Creation and detection of vector vortex modes for classical and quantum communication. J. Light. Technol. 36, 292–301 (2018).

45. 45.

Maurer, C., Jesacher, A., Fürhapter, S., Bernet, S. & Ritsch-Marte, M. Tailoring of arbitrary optical vector beams. New J. Phys. 9, 78 (2007).

46. 46.

Rong, Z.-Y., Han, Y.-J., Wang, S.-Z. & Guo, C.-S. Generation of arbitrary vector beams with cascaded liquid crystal spatial light modulators. Opt. Express 22, 1636 (2014).

47. 47.

Rosales-Guzmán, C., Bhebhe, N. & Forbes, A. Simultaneous generation of multiple vector beams on a single slm. Opt. Express 25, 25697–25706 (2017).

48. 48.

Otte, E., Rosales-Guzmán, C., Ndagano, B., Denz, C. & Forbes, A. Entanglement beating in free space through spin–orbit coupling. Light. Sci. & Appl. 7, 18009 (2018).

49. 49.

Michihata, M., Hayashi, T. & Takaya, Y. Measurement of axial and transverse trapping stiffness of optical tweezers in air using a radially polarized beam. Appl. optics 48, 6143–6151 (2009).

50. 50.

Zhong, M.-C. et al. Optical trapping of core-shell magnetic microparticles by cylindrical vector beams. Appl. Phys. Lett. 105, 181112 (2014).

51. 51.

Skelton, S. et al. Trapping volume control in optical tweezers using cylindrical vector beams. Opt. letters 38, 28–30 (2013).

52. 52.

Donato, M. et al. Optical trapping of nanotubes with cylindrical vector beams. Opt. letters 37, 3381–3383 (2012).

53. 53.

Richards, B. B. Richards and e wolf. Proc. R. Soc. London, Ser. A 253, 358 (1959).

54. 54.

Roxworthy, B. J. & Toussaint, K. C. Jr. Optical trapping with p-phase cylindrical vector beams. New J. Phys. 12, 073012 (2010).

55. 55.

Zhan, Q. & Leger, J. R. Focus shaping using cylindrical vector beams. Opt. Express 10, 324–331 (2002).

56. 56.

Otte, E., Tekce, K. & Denz, C. Tailored intensity landscapes by tight focusing of singular vector beams. Opt. express 25, 20194–20201 (2017).

57. 57.

Kozawa, Y. & Sato, S. Optical trapping of micrometer-sized dielectric particles by cylindrical vector beams. Opt. Express 18, 10828–10833 (2010).

58. 58.

Nieminen, T. A., Heckenberg, N. R. & Rubinsztein-Dunlop, H. Forces in optical tweezers with radially and azimuthally polarized trapping beams. Opt. letters 33, 122–124 (2008).

59. 59.

Milione, G., Sztul, H., Nolan, D. & Alfano, R. Higher-order poincaré sphere, stokes parameters, and the angular momentum of light. Phys. review letters 107, 053601 (2011).

60. 60.

Holleczek, A., Aiello, A., Gabriel, C., Marquardt, C. & Leuchs, G. Classical and quantum properties of cylindrically polarized states of light. Opt. express 19, 9714–9736 (2011).

61. 61.

Sinjab, F. et al. Holographic optical trapping raman micro-spectroscopy for interacting live cells. bioRxiv 292334 (2018).

62. 62.

Naidoo, D. et al. Controlled generation of higher-order poincaré sphere beams from a laser. Nat. Photon. 10, 327–332 (2016).

63. 63.

Ndagano, B., Brüning, R., McLaren, M., Duparré, M. & Forbes, A. Fiber propagation of vector modes. Opt. express 23, 17330–17336 (2015).

64. 64.

Ndagano, B. et al. Characterizing quantum channels with non-separable states of classical light. Nat. Phys. 13, 397–402 (2017).

65. 65.

Mitchell, K. J., Turtaev, S., Padgett, M. J., Čižmár, T. & Phillips, D. B. High-speed spatial control of the intensity, phase and polarisation of vector beams using a digital micro-mirror device. Opt. express 24, 29269–29282 (2016).

66. 66.

Leach, J. et al. 3d manipulation of particles into crystal structures using holographic optical tweezers. Opt. Express 12, 220–226 (2004).

## Acknowledgements

The authors acknowledge financial support from the South African National Research Foundation and the DST-CSIR scholarship fund.

## Author information

### Affiliations

1. #### School of Physics, University of the Witwatersrand, Private Bag 3, Wits, 2050, South Africa

• Nkosiphile Bhebhe
• , Carmelo Rosales-Guzmán
• , Valeria Rodriguez-Fajardo
•  & Andrew Forbes
2. #### Mechanical Engineering, Massachusetts Institute of Technology, 33 Massachusetts Ave, Cambridge, MA, 02139, USA

• Peter A. C. Williams

### Contributions

Experiments were performed by N.B., P.A.C.W. and C.R.G. All authors contributed to the data analysis and interpretation of results. A.F, C.R.G., V.R.F. and N.B. wrote the manuscript. A.F. supervised the project.

### Competing Interests

The authors declare no competing interests.

### Corresponding author

Correspondence to Andrew Forbes.