Abstract
Reconstitution of membrane proteins in artificial membranes is an essential prerequisite for functional studies that depend on the context of an intact membrane. While straight-forward protocols for reconstituting proteins in small unilamellar vesicles were developed many years ago, it is much more difficult to prepare large membranes containing membrane proteins at biologically relevant concentrations. Giant unilamellar vesicles (GUVs) represent a model system that is characterised by low curvature, controllable tension, and large surface that can be easily visualised with microscopy, but protein insertion is notoriously difficult. Here we describe a convenient method for efficient generation of GUVs containing functionally active SNARE proteins that govern exocytosis of synaptic vesicles. Preparation of proteo-GUVs requires a simple, in-house-built device, standard and inexpensive electronic equipment, and employs a straight-forward protocol that largely avoids damage of the proteins. The procedure allows upscaling and multiplexing, thus providing a platform for establishing and optimizing preparation of GUVs containing membrane proteins for a diverse array of applications.
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Introduction
Giant unilamellar vesicles (GUVs) represent artificial vesicles with a diameter of >1 µm. Originally introduced many years ago by Reeves and Dowben1, GUVs have only recently become more popular for studying the properties of membrane proteins2. Their main advantages are: (i) GUVs can be conveniently visualised with basic light microscopy techniques (in contrast to smaller vesicles); (ii) GUVs, even if immobilised, are largely free of curvature stress provided that the surface contact area is comparatively small; and (iii) GUVs can be easily manipulated, and their membrane tension can be precisely controlled. However, due to the large size of GUVs imaging of the membrane surface requires 3D scanning and thus limits the time resolution of the experiment. Moreover, preparation of GUVs with a desired protein and lipid composition can be challenging3: There is no universal protocol because different procedures may be required to achieve optimal results depending on, among other factors, protein, lipid, and buffer compositions.
Traditionally, GUVs are formed with a gentle hydration method1, but this method is rather slow and inefficient3. A major improvement was introduced by Angelova and Dimitrov4 with the electroformation technique. In this method, vesicles are formed from a dried lipid film while an external electric field is applied. Initially, lipid films were deposited directly on platinum (Pt) electrodes4. Later this technique was modified to work with glass slides coated with a conductive surface (indium tin oxide, ITO)5. Additional GUV preparation methods have also been developed including osmotic shock6, gel assisted swelling7,8, peptide-induced fusion of smaller precursor vesicles9, detergent-mediated reconstitution10, droplet-transfer method11, or an inkjet-based formation12.
Preparation of protein-containing GUVs (proteo-GUVs) has been a particularly challenging task due to the fact that GUV-formation usually includes steps that can denature proteins. Such steps may include: (i) formation of a lipid mixture from lipids diluted in organic solvents, (ii) drying, and (iii) formation of vesicles in a salt-free environment (but note that there are ongoing efforts to optimize protocols utilizing physiological ionic strength buffers, see e.g. ref.13). Despite these drawbacks, GUVs are increasingly used for in vitro reconstitution of membrane proteins and for the study of membrane remodelling, for instance for studying bacteriorhodopsin14, ion channels13,15,16, or exocytosis of synaptic vesicles17,18,19.
Nevertheless, the preparation of proteo-GUVs still requires time-consuming optimization as protocols are frequently difficult to reproduce between laboratories (own experience and personal communication with other researchers in the field), largely because not all variables affecting the outcome are controlled and optimised. This includes, for instance, design of the electroformation chamber including slide resistance when using ITO slides, spacer thickness (that is necessary for calculating the electric field), and for Pt electrodes information about the thickness and the axial distance between the wires. Moreover, the parameters of the applied electric field are critical for the outcome, with the results depending on the chamber geometry and the precise voltage-time profile of the applied electric field.
Here we report a convenient protocol for the preparation of proteo-GUVs containing functionally active neuronal SNARE (soluble N-ethylmaleimide-sensitive factor activating protein receptor) proteins for the study of membrane fusion in vitro. SNARE proteins represent a superfamily of small, mostly membrane-anchored proteins that catalyse the fusion of membranes in all eukaryotic cells. In neurons, exocytosis of synaptic vesicles is mediated by the SNARE proteins syntaxin-1A and SNAP-25 present at the plasma membrane, and synaptobrevin-2 present on the vesicles. Our protocol is straightforward and requires only a simple and affordable, in-house-built setup, therefore it can be easily adapted for other proteins and lipid compositions.
Results
Setup Design
Electroformation of GUVs can be performed by applying an alternating electric field in the formation chamber that either consists of two glasses coated with conductive material (such as ITO) that are separated by a spacer (see e.g. refs18,20,21,22; Fig. 1a), or that contains two Pt electrodes (presented in this work; Fig. 1b). In the first approach, the parameters that critically influence the electroformation are the electrical resistance of the ITO coat and the distance between the two conductive surfaces. When Pt wires are used for preparation of GUVs, the two important parameters are the axial distance and the thickness of the two parallel electrodes. In both cases, additional parameters have a strong influence on GUV formation such as chamber volume, chamber cleanliness and deterioration due to repeated use, concentration of lipids, the method of lipid drying, and the composition of the buffer used for electroformation.
Previously, we prepared SNARE-containing GUVs using ITO-coated glasses18,20 (see also Fig. 1a). However, GUV formation was not easily reproducible and yielded relatively small GUVs (often below 5 µm in diameter), prompting us to work out a more reliable protocol that would yield larger GUVs. We therefore designed a Pt wire-based electroformation chamber (in short Pt chamber; Figs 1b and 2a; other designs are presented for example in refs13,23). The main idea was to design a chamber that could be effectively cleaned with organic solvents (to remove residual lipids), and that allows for easy monitoring of GUV formation (compatibility with a standard Zeiss microscopy stage). For this purpose, a largely chemically inert PTFE (polytetrafluoroethylene) was chosen as the main material for the chamber with two Pt wires (0.5 mm in diameter) embedded close to the chamber bottom. The dimensions of the chamber were chosen to allow for sealing with a standard size (25 mm in diameter) microscopic coverslip and to fit, together with the wiring, on a microscopy stage (Figs 1b and 2a,e). For electroformation we used a digital function generator (Velleman PCGU1000), connected via USB to a Windows PC (Fig. 1c). This function generator is inexpensive in comparison to other (usually stand-alone) laboratory function generators, and the output voltage waveform can be easily programmed within the accompanying software (PcLab2000SE, Velleman). The Pt chamber was connected to the function generator using cable with BNC connector and pin socket. In this setup socket pitch of 2.54 mm fits the 2.5 mm axial distance of Pt electrodes, while for the ITO chamber the latter were replaced with crocodile clips (Fig. 1c). Additionally, by using BNC Y-splitters, multiple chambers can be connected to one function generator. In conclusion, the whole electroformation setup consists of a PC, a function generator, connecting cables, and electroformation chambers (Fig. 1c). One electroformation chamber can be then placed on a microscope (as shown in Fig. 2e, to allow live monitoring, see Supplementary Video 1), while others can be placed for stability in a suitable stand (like the one made from the polyethylene foam shown in Fig. 2d).
Formation of GUVs in a Pt chamber
The most common approach for formation of GUVs containing transmembrane proteins is to start with small liposomes reconstituted with membrane proteins using standard protocols, e.g. using detergent removal by size exclusion chromatography3,20,24 (Fig. 3a). These proteoliposomes are deposited on the conductive surface in the electroformation chamber and dried in order to remove the aqueous buffer. For instance, for preparing SNARE-GUVs with a Pt chamber 5–7 × 1 µl drops of SUVs are deposited on each Pt wire (10–14 drops/Pt chamber) and dried under vacuum for around 30 minutes (Fig. 3a). Next, the Pt chamber is sealed with a coverslip (25 mm diameter, coated with β-Casein to prevent bursting of GUVs making contact with the glass surface) and a silicone glue (see photo in Fig. 2c). The sealed chamber is then connected to the function generator and filled with an electroformation solution — typically water with sucrose (we used 800 µl of 200 mM sucrose solution in each chamber; see photos in Fig. 2d and e). Immediately afterwards electroformation is started by switching on the AC field. In our hands, the best GUV quality and highest protein activity was obtained when electroformation was performed for 1 h at 10 Hz, 2.2 Vpp (peak-to-peak voltage, sine wave shape), followed by a detachment phase (detaching GUVs from Pt wires into the solution) of 30 min at 2–4 Hz, 2.2 Vpp (sine wave shape, Fig. 3b and Supplementary Video 1). After detachment, GUVs are collected by pipetting with a cut 1 ml micropipette tip and transferred directly to the imaging chamber, or stored refrigerated for up to a week in a microcentrifuge tube (Fig. 3a).
GUV quality analysis — vesicle diameter and efficiency of protein reconstitution
Depending on the biological problem to be studied, the average diameter of GUVs may be critical. For instance, in some experiments handling and visualization of larger GUVs may be beneficial. The SNARE-GUVs prepared with a Pt chamber have diameters ranging from around 5 to 30 µm (Fig. 4a and c). Thus, the average diameter (13.5 µm, Fig. 4a) is substantially larger than that of the same GUVs prepared with ITO slides (5.8 µm)18. Another parameter critical for the assessment of GUV quality is the amount of protein incorporated in the membrane. In GUVs prepared with a Pt chamber we observe efficient protein incorporation by monitoring fluorescence intensity of Texas Red labelled proteins in the GUV membrane (Fig. 4b). By comparing these intensities with those of labelled lipid (for details see Materials and Methods and ref.18), the protein concentration in the membrane can be estimated (see histogram in Fig. 4b). Although the protein to lipid ratio showed some variability, there was no correlation with the size of the GUVs.
GUV quality analysis — protein activity
SNARE proteins catalyse most membrane fusion reactions in eukaryotic cells25. Therefore, the best test for their activity upon membrane reconstitution and vesicle formation is to perform fusion assays26. Here we measured fusion using a lipid mixing assay18,27. In this experiment, immobilised GUVs containing labelled lipid NBD-PE as fluorescence donor and a stabilised complex of plasma membrane SNARE proteins28, were incubated with SUVs containing Lissamine Rhodamine-PE (Rho-PE) as fluorescence acceptor and the vesicular SNARE (schematic illustration in Fig. 4d). Upon SNARE-mediated membrane fusion, these two labels are in the same membrane and undergo Förster resonance energy transfer (FRET), causing quenching of NBD. If Rho is then bleached, a corresponding recovery of the NBD fluorescence intensity is observed (Fig. 4e, red). As a control for the specificity of this reaction, we used a synaptobrevin mutant (Δ84)29 that stops the fusion reaction at the docked state, preventing mixing of lipids and thus reducing FRET (Fig. 4e, grey).
Discussion
Here we describe a convenient procedure for preparing proteo-GUVs containing SNARE-proteins of the presynaptic plasma membrane using in-house-built devices. The electroformation chamber described here is made from PTFE and thus can be cleaned with organic solvents. Moreover, the chamber allows for directly monitoring the formation of GUVs under a microscope. Additionally, the function generator used in this study can be easily programmed, allowing for testing of multiple electroformation protocols (a crucial step when establishing a protocol for proteo-GUV formation).
The protocol described here is convenient and avoids some of the problems associated with other methods. For instance, the procedures involving osmotic shock6 require repetitive drying-rehydration cycles, which are likely to be detrimental for maintaining membrane proteins in a functional state. Furthermore, gel-assisted swelling30 was reported to yield GUVs with altered mechanical properties31. Another possibility is to reconstitute proteins into the preformed GUVs with the aid of low concentrations of a detergent10, yet it requires extensive optimization of detergent type and concentration3, and it is very difficult to achieve efficient protein insertion while maintaining the GUVs intact. For special purposes, i.e. when membrane asymmetry is required, GUVs may be prepared with inkjet method12, however these technique requires a more specialised, and expensive equipment.
We conclude that our protocol offers a convenient method for the preparation of large GUVs containing moderate-high concentrations of membrane proteins. The yield of high-quality GUVs is comparably high, and only a single drying step is required, helping to preserve protein activity. For sensitive proteins, additional protection during the drying process may be necessary, for instance by adding disaccharides or ethylene glycol3,32,33,34.
Materials and Methods
Materials
Lipids were purchased from Avanti Polar Lipids: DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), 18:1 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl)), DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), cholesterol (ovine wool), 18:1 NBD-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)), and 18:1 Liss Rho-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)). Texas Red - coupled DHPE was purchased from Invitrogen. Lipophilic tracers — DiO and DiD, NeutrAvidin, biotinylated bovine serum albumin, and Texas Red maleimide (for protein labelling) were from Thermo Fisher Scientific. β-Casein (from bovine milk) was from Sigma-Aldrich, and Picodent Twinsil® 22 (silicone glue) was from Picodent.
Protein purification
Synaptic SNARE proteins: syntaxin-1A (183–288)35, SNAP-25 (cysteine free36 and S130C37), synaptobrevin-2 (wild type38 and Δ84 mutant39), and synaptobrevin-2 fragment (49–96)28; were derived from Rattus norvegicus and were expressed as pET28a constructs in Escherichia coli strain BL21 (DE3). Single proteins were purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) and subsequent ion exchange chromatography on an Äkta system (GE Healthcare) with either MonoQ (syntaxin-1A and SNAP-25) or MonoS columns (synaptobrevin). For the proteins containing transmembrane region, i.e. syntaxin and synaptobrevin full length, buffer containing 1% CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, from Anatrace) or 1% octyl β-D-glucopyranoside (Glycon) was used, respectively. The assembly of the plasma membrane SNARE complex (consisting of syntaxin, SNAP-25, and synaptobrevin fragment 49–96) — a so called ΔN complex28 — was done by mixing the monomers overnight at 4 °C, followed by purification of the complex by ion exchange chromatography (MonoQ column) in a buffer containing CHAPS as described28. Fluorescence labelling of SNAP-25 (S130C) was carried out using Texas Red maleimide according to manufacturer’s instructions. Labelled ΔN complex was formed by replacing SNAP-25 with a S130C mutant labelled with Texas Red.
Preparation of small unilamellar vesicles and fluorescent labelling of vesicles
Small unilamellar vesicles (SUVs) containing SNARE proteins (the plasma membrane SNARE complex or synaptobrevin) were prepared by co-micellization followed by size exclusion chromatography as described before18 with the following lipid composition: DOPC, DOPE, DOPS, and cholesterol at a molar ratio of 5:2:2:1. For immobilization of GUVs 1 mol% of DOPE was replaced with biotinyl-cap-PE, and for fluorescent labelling 1 mol% of DOPC was replaced with a membrane dye DiO or DiD. In case of lipid mixing experiments, GUVs contained 1.5 mol% NBD-PE whereas the SUVs were labelled with 1.5 mol% of Rho-PE. Protein was reconstituted at protein to lipid ratio of 1:1000 (SNARE complex in GUVs) or 1:500 (synaptobrevin in SUVs). Liposomes were formed in a buffer consisting of 20 mM HEPES/KOH pH 7.4, and 150 mM KCl, with a final lipid concentration of ∼0.7 mM determined according to ref.40.
Preparation of giant unilamellar vesicles
GUVs containing SNARE proteins were prepared from vacuum-dried proteo-SUVs with the electroformation procedure using an in-house-built Pt electrode electroformation chamber (referred to as Pt chamber, see Fig. 2). The detailed GUV preparation protocol is described in the Results section.
Prior to use, the Pt chamber was cleaned by bath sonication (around 5–10 min) in ethanol and subsequently in chloroform. For sealing of the chamber, microscopy coverslips (25 mm in diameter) were used, that were first cleaned with ethanol and isopropanol, then coated with β-Casein (3 mg/ml, 5 min), and finally rinsed with water and dried.
Microscopy imaging and data analysis
The formation of GUVs was directly monitored at low magnification in the electroformation chamber with an epifluorescence microscope. For visualization in higher magnification, GUVs were collected after the electroformation procedure and transferred to the imaging chamber containing a coverslip functionalised with biotinylated BSA and neutravidin18, and imaging buffer (20 mM HEPES/KOH pH 7.4, 150 mM KCl, 1 mM MgCl2, at least 1.5 × volume of the GUV solution to be added). GUVs were allowed to settle for around 30 min prior to imaging, resulting in surface attachment. Microscopy imaging was done with a Zeiss Axiovert 200 epifluorescence microscope or with a Zeiss LSM 780 confocal microscope.
The efficiency of protein reconstitution was determined as described in ref.13, following the detailed protocol described in ref.18, by comparing membrane fluorescence intensity of Texas Red labelled ΔN complex with those of known concentration of Texas Red labelled DHPE. Bulk lipid mixing experiments were performed essentially as described in ref.18. Image analysis was performed in Fiji41 with self-written scripts18,42,43.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We are grateful to Dr. Susann Spindler and Martin Kaller (Erlangen, Germany) for helpful discussions. We thank the Workshop for Precision Mechanics and Electronics Workshop of the Max Planck Institute for Biophysical Chemistry (Göttingen, Germany) for preparing GUV chambers according to our design. This work was supported by US National Institutes of Health grant No. 2 P01 GM072694 (to R.J.).
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A.W. and R.J. designed and discussed the experiments. A.W. and L.J. designed the experimental setup, including the electroformation chamber. A.W. performed the experiments. A.W. and R.J. wrote the manuscript. All authors edited the manuscript.
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Witkowska, A., Jablonski, L. & Jahn, R. A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation. Sci Rep 8, 9422 (2018). https://doi.org/10.1038/s41598-018-27456-4
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DOI: https://doi.org/10.1038/s41598-018-27456-4
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