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Selective uptake and sensing of nitrate in poly(3,4-ethylenedioxythiophene)

Scientific Reportsvolume 7, Article number: 16581 (2017) | Download Citation


Nitrogen (N) as a nutrient, in the form of nitrate (NO3 ), is essential for plant growth. Chemical fertilizers are used to increase crop yields, but overuse can lead to forms of environmental pollution necessitating methods to detect and monitor the level of NO3 in-situ in agricultural soils. Herein we report for the first time the NO3 selectivity of the inherently conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT). This selectivity occurs when PEDOT thin films are exposed to an aqueous environment containing not only NO3 , but a mixture of other ions present in concentrations (ppm) typical of real agricultural soil. The PEDOT sensitivity to absorb NO3 from solution is determined to be <1 ppm.


In order to enhance the effectiveness of the soil and improve quality and yield in agricultural production, fertilizers and chemical materials are typically utilised to assist in the global need for food, feed, fuel and fibre1,2. With the continued use of chemicals in modern industries and agriculture, detection and monitoring of anion contamination is increasingly important. For instance, nitrate (NO3 ) levels above 10 mg/L (10 ppm) in groundwater, originating from nitrogen-based fertilizers, have been linked to health issues for humans3.

The promising features of conducting polymers (CPs) have attracted significant attention from scientists aiming to improve the conductivity of CPs as synthetic metals4. Among the known CPs, poly(3,4-ethylenedioxythiophene) (PEDOT) has been considered a desirable material in a variety of applications including solar cells5, organic light emitting devices6 and medical devices7 owing to its unique characteristics of excellent conduction and electro-optical properties. Developments in the synthesis of CPs have shown that PEDOT becomes highly conductive when doped and/or with certain post-treatment8. In principle, the pristine PEDOT is obtained indirectly via de-doping, hence electrochemical techniques such as cyclic voltammetry are used to discharge or recharge CPs in the presence of an electrolyte (salt solution containing anions)9. As a response to electrochemical oxidation or reduction, the electrical and optical properties of PEDOT change significantly due to the doping or de-doping process (anion uptake or release)10,11. This phenomenon makes PEDOT an ideal substance for controlled drug release applications12, however anion uptake of this CP can be employed in other advanced applications such as sensors. For instance, Zampetti et al. reported a highly sensitive sensor using an ultra-thin layer of PEDOT to monitor NO and NO2 gases in breath and eventually to monitor asthma13. In another study Luo and co-workers used PEDOT as a matrix of a nanocomposite to create an electrochemical sensor for detection of nitrite14. Gokhale et al. immobilised nitrate reductase enzymes within PEDOT nanowires, where the enzymes reduce nitrate to nitrite, and the resultant electrons are transferred via methyl viologens in solution to the PEDOT bioelectronic sensor15.

In recent years, various materials and methods have been trialled for the purpose of developing a nitrate sensor for agricultural purposes16,17,18,19. Coupling LED light sources in a spectrophotometric analysis allows for colour-developing agents to be mixed with extracted water samples to yield colour intensity vs concentration curves20. These calibration curves then allow for determination of anion content within the extracted water. Weber and co-workers utilised an impedance technique in a two electrode flow cell arrangement with extracted water from soil added to a known soil mix21. The dielectric constant of the known soil mix when dry is compared to the same mix when wetted with different total salt content. Without providing details of how, this process is reported to be adaptable to specifically sense NO3 . More recently from the same research group, Ali et al. reported an electrochemical sensor comprising a working electrode of electrospun PEDOT nanofibers decorated with graphene oxide on gold substrates22. Enzymes to convert nitrate to nitrite are immobilised on the graphene oxide, hence rendering an electrochemical reaction that can be determined via electrochemical impedance spectroscopy. This method requires a nitrate sensitive working electrode, plus a counter and reference electrode. Further, Higson and co-workers, in their excellent review of anion selective sensors, highlight that doping CPs with anion-recognition sites presents an ideal scenario for simple detection of specific anions23. Many of the anion sensing-CP studies to date have focused on polypyrrole24,25 and its derivatives26 using electrochemical processes for detection. In the present work, we fabricated simple PEDOT-Tosylate thin films via Vapour Phase Polymerisation (VPP)27, electrochemically de-doped the PEDOT to remove most of the Tosylate, then selectively re-doped it with NO3 directly from a mixed ion solution extracted from agricultural soil. This re-doping was done using concentrations of NO3 in the ppm range, without any external electrical stimulus, nor with the need for engineering anion recognition sites. Selectivity for NO3 was observed to be inherent for VPP PEDOT.

Results and Discussion

The initial electrochemical reduction process leads to the sheet resistance (Rs) of the PEDOT increasing - rendering the PEDOT lower in conductivity and higher in opacity, due to the decreased number of anions within28. Interestingly, the removal of anions from PEDOT can also be observed in the absence of any electrical stimuli. As-prepared PEDOT immersed in Milli-Q water (no Tosylate in solution) displayed an increase in optical opacity in line with anion removal and PEDOT reduction (Fig. 1a). When (electrochemically) reduced PEDOT is exposed to electrolyte containing NO3 it appears more optically transparent to the eye, and is verified by UV-Vis spectroscopy (Fig. 1b). This occurs without the application of any external electric potential to drive electrochemical insertion of the anions, indicating the movement of anions in and out of the PEDOT occurs via diffusion. The diffusion is hypothesised to arise from the chemical potential difference between PEDOT and electrolyte with respect to the different anion concentration in each phase (PEDOT vs electrolyte).

Figure 1
Figure 1

Properties of reduced PEDOT exposed to NO3−. Absorption spectra of (a) PEDOT as-prepared and after reduction by Milli-Q water, (b) electrochemically reduced PEDOT before and after exposure to NO3 (c) neutral and bipolaron of PEDOT- as prepared, electrochemically reduced and reduced PEDOT after exposure to NO3 , and (d) measured sheet resistance (Ω/□) of PEDOT before and after treatment.

Figure 1c and d follow the changes in optical and electrical properties of the PEDOT through the various steps. The as-prepared PEDOT has the lowest Rs, which increases slightly upon exposure to Milli-Q water, and significantly when electrochemically reduced. In both cases anions are being released from the PEDOT. When the reduced PEDOT is exposed to the nitrate solution Rs decreases. This highlights the re-uptake of anions into the PEDOT film, thus doping or oxidising the PEDOT. The chemical analysis of the polymer film via X-ray photoelectron spectroscopy (XPS) confirmed the anion uptake by reduced PEDOT within this process (Figure S1).

The ion chromatography (IC) analysis of real water extracted from an agricultural field showed that in practice there is a mixture of ions in low concentrations (Fig. 2a). In fact, while there is a variation in NO3 concentration, both the Cl and SO4 concentrations are significant and Br is detectable. The use of three distinct samples in the current study is in contrast with other studies that rely on one sample diluted to various NO3 concentrations22. Dilutions such as these not only change the NO3 concentration but also the background ion content. Owing to the fact that PEDOT takes up anions from the surrounding aqueous environment, the performance and sensitivity to specific ions was also evaluated. The chemical analysis of the PEDOT films, using XPS, revealed that only NO3 (in varying concentrations) was absorbed by PEDOT (Figs 2b and S2). The XPS spectra showed an absence of Cl, SO4 or Br (Figure S4 presents analysis of the S 2p fine scan). Comparing the XPS data for the atomic percentage of N with the NO3 concentration in solution from IC shows that the uptake of N is related to the concentration of NO3 in solution (Fig. 2c). The error bars in Fig. 2c and d represent the measurement errors in determining the atomic percentage and relative change in conductivity. Samples were found to be within these measurement errors.

Figure 2
Figure 2

Exposure of reduced PEDOT to water extracted from agricultural soil. (a) Ion chromatography (IC) analysis of anions (see legend) present in the water samples (1 to 3) extracted from the field, (b) chemical analysis of treated PEDOTs using X-ray photoelectron spectroscopy (XPS), (c) atomic percentage of N absorbed by reduced PEDOT, and (d) calculated change of PEDOT’s sheet resistance after exposure to the extracted water samples 1 through 3.

The UV-Vis-NIR spectrophotometer was used to investigate the oxidation of PEDOT as a result of taking up anions (Figure S3). Although providing confirmation of the sensitivity of PEDOT to low concentration of anions it appears that the optical technique is not sensitive enough to correlate the oxidation level of PEDOT to anion concentration, as all spectra of treated films were almost the same. However, the comparison of the change in sheet resistance of treated PEDOT films illustrates a correlation with the concentration of NO3 in solution (Fig. 2d).

The correlation between N present and the change in electrical properties in the exposed PEDOT with the NO3 concentration in solution was tested over a wider range of NO3 concentrations (<0.05 up to 280 ppm). The chemical analysis of these solutions is provided in Fig. 3a (IC analysis). NO3 is the major component of solutions A, B and C, whereas in D and E, Cl, SO4 and NO3 all appear in concentrations <1 ppm. XPS analysis of the corresponding exposed PEDOT samples reveals a similar correlation between N in PEDOT and NO3 in solution (Fig. 3b). The lower limit of N uptake into the PEDOT is observed to occur between 0.02 and 0.2 ppm NO3 concentration. At 0.02 ppm it cannot be concluded that N is present in the PEDOT merely that it is below the measurement resolution of the XPS. The lower limit is demonstrated however by the measured change in sheet resistance of the PEDOT from its reduced state and after exposure to NO3 (Fig. 3c). The percentage change for the 0.02 and 0.2 ppm NO3 exposure is comparable. When comparing the absolute uptake of N in PEDOT from the agricultural soil water samples and the NO3 samples, the NO3 samples uptake more N. This is rationalised as being due to the presence of only one cation, Na+, in solution at the same concentration as the NO3 . Conversely the extracted agricultural water has a complex mix of anions and cations, in most cases at concentrations exceeding that of the NO3 . The complex mix of ions is hypothesised to retard the uptake of N into PEDOT.

Figure 3
Figure 3

Uptake of differing concentrations of NO3− by reduced PEDOT. (a) Ion Chromatography analysis of anion content in samples A through E, showing decreasing [NO3 ] and background [Cl] and [SO4 ], (b) atomic percentage of N taken up by the reduced PEDOT and (c) calculated change of PEDOT’s sheet resistance, after exposure to different [NO3 ] solutions (log scale).

It is necessary to compare the sensitivity of the PEDOT material for NO3 with other more commonly used techniques, such as IC which is generally used as the benchmark technique for determining ion concentration(s). This technique has a typical minimum measurable concentration of 0.05 ppm. Otterpohl and co-workers29 compare a range of techniques to measure NO3 in the presence of high concentrations of Cl. The reported minimum measurable concentration across those studied was between 0.2 and 2 ppm. An electrochemical process was employed by Badea et al. using coated platinum electrodes30. While the minimum [NO3 ] was not reported, concentrations of 4 to 44 ppm NO3 was measured in mixed ion solutions. Wood and co-workers31 developed a nitrate selective electrode based upon N,N,N-triallyl leucine betaine chloride in a cross-linked polymer binder. This sensor covered a concentration range of 0.47 to 16 ppm NO3 . A downside to electrochemical measurements such as these is the need for a reference electrode to be immersed in the same environment. In contrast, a PEDOT film is easily fabricated, requires no reference electrode, and demonstrates selectivity for NO3 across a comparable concentration range to other techniques even in the presence of other anions. The hypothesised origins of this selectivity relates to the π,π-enhanced anion-π interactions described by Dawson et al.32. This is a reasonable assumption given a PEDOT film is well described as a supramolecular structure with strong π-bonding and an affinity for anions.

In summary, the conductive polymer PEDOT is observed to show specific selectivity for NO3 uptake from aqueous solutions. The NO3 dopes PEDOT via a passive process (not requiring external driving voltages or electric fields) in preference to other anions even if present in equal or higher concentrations in solution. In the case of neat NO3 solutions, N is detected in PEDOT from solutions as low as 0.2 ppm NO3 . In practice the change in electrical properties of the PEDOT could be used to detect the concentration of NO3 from solution. Such sensing is of interest in relation to aqueous environments where NO3 is present, such as agricultural water in soil. These novel findings extend the function of PEDOT beyond its typical use as a flexible conductive and optoelectronic p-type material.

Materials and Methods

Fe(III) tosylate was received from H. C. Starck as a 54 wt.% solution in butanol (Baytron CB 54). 3,4-Ethylenedi-oxythiophene (EDOT) monomer, lithium perchlorate, sodium nitrate and sodium chloride and the block copolymers poly(ethylene glycol−propylene glycol−ethylene glycol) (PEG-PPG-PEG) in Mw = 5800 were obtained from Sigma-Aldrich. All chemicals were used without further purification.

The conductivity was calculated using σ = (Rs.t)−1, where Rs is the sheet resistance and t is the thickness of the PEDOT film. Rs was measured using a Jandel four point probe, and film thickness was measured with a Bruker DektakXT profilometer with 12.5 μm stylus under a 3 mg load. The surface chemistry of polymer films was analysed using an SPECS (SAGE, Phoibos 150-HSA) X-ray photoelectron spectroscopy (XPS) system fitted with a non-monochromated Al anode, with a power of 200 W and a base pressure of 2 × 10−6 Pa. Curve fitting was performed with Casa XPS (Neil Fairley, U.K.), using a linear background. Spectra were charge corrected relative to the aliphatic carbon peak at 285 eV.

PEDOT films were synthesized on cleaned quartz slides as substrate using the vacuum-VPP process. Prior to polymerisation, substrates were air plasma treated (PDC-32G, Harrick Inc.) for 2 min. The oxidant film was directly spin-coated (400B-6NPP, Laurell Technologies Inc.) onto substrate at 1500 rpm for 25 s and then placed on a hotplate set to 70 °C for 30 s to evaporate the solvents. Samples were then removed from the hotplate and placed into a polymerisation chamber (Binder Vacuum Oven-VD 115) set to 35 °C. After polymerisation, PEDOT films were carefully washed with ethanol to remove any unreacted species and existing oxidant solution. Since PEDOT is almost fully doped via the VPP process, a three electrode system in electrochemical cell was employed to electrochemically reduce the PEDOT films in the presence of a standard Ag/AgCl electrode (3.8 M KCl) as the reference electrode, in order to remove most of the anions from the PEDOT films.

Three different extracted water samples from the field were provided by Sentek Pty Ltd. Provided samples were filtered using Acrodisc Syringe Filter 0.45 μm Supor Membrane to remove particulate material, and then electrochemically reduced PEDOT films were exposed to the samples. PEDOT films were kept in the water samples for one hour, and then dried by air. Treated PEDOT films were investigated before and after treatment using various techniques, investigating the electrical and optical properties, in addition to their chemical composition.

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  1. 1.

    Bashan, Y., de-Bashan, L. E., Prabhu, S. R. & Hernandez, J. P. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant Soil 378, 1–33 (2014).

  2. 2.

    Schroeder, J. I. et al. Using membrane transporters to improve crops for sustainable food production. Nature 497, 60–66 (2013).

  3. 3.

    Knobeloch, L., Salna, B., Hogan, A., Postle, J. & Anderson, H. Blue babies and nitrate-contaminated well water. Environ Health Perspect 108, 675 (2000).

  4. 4.

    MacDiarmid, A. G. “Synthetic Metals”: A Novel Role for Organic Polymers (Nobel Lecture). Angew Chem Int Ed 40, 2581–2590 (2001).

  5. 5.

    Kim, Y. H. et al. Highly conductive PEDOT: PSS electrode with optimized solvent and thermal post‐treatment for ITO‐free organic solar cells. Adv Funct Mater 21, 1076–1081 (2011).

  6. 6.

    Kim, Y. H. et al. Achieving High Efficiency and Improved Stability in ITO‐Free Transparent Organic Light‐Emitting Diodes with Conductive Polymer Electrodes. Adv Funct Mater 23, 3763–3769 (2013).

  7. 7.

    Ludwig, K. A., Uram, J. D., Yang, J., Martin, D. C. & Kipke, D. R. Chronic neural recordings using silicon microelectrode arrays electrochemically deposited with a poly (3,4-ethylenedioxythiophene)(PEDOT) filmThis work was supported by the Center for Wireless Integrated Microsystems NSF EEC-9986866 and the Whitaker Foundation. J Neural Eng 3, 59 (2006).

  8. 8.

    Mueller, M. et al. Vacuum vapour phase polymerization of high conductivity PEDOT: Role of PEG-PPG-PEG, the origin of water, and choice of oxidant. Polymer 53, 2146–2151 (2012).

  9. 9.

    Johanson, U., Marandi, M., Tamm, T. & Tamm, J. Comparative study of the behavior of anions in polypyrrole films. Electrochim Acta 50, 1523–1528 (2005).

  10. 10.

    Lock, J. P. et al. Electrochemical investigation of PEDOT films deposited via CVD for electrochromic applications. Synth Metals 157, 894–898 (2007).

  11. 11.

    Martinez J. G., Berrueco B. & Otero T. F. Deep reduced PEDOT films support electrochemical applications: biomimetic color front. Front Bioeng Biotechnol 3 (2015).

  12. 12.

    Svirskis, D., Travas-Sejdic, J., Rodgers, A. & Garg, S. Electrochemically controlled drug delivery based on intrinsically conducting polymers. J Control Release 146, 6–15 (2010).

  13. 13.

    Zampetti, E. et al. A high sensitive NO2 gas sensor based on PEDOT–PSS/TiO2 nanofibres. Sensor Actuat B 176, 390–398 (2013).

  14. 14.

    Xu, G., Liang, S., Fan, J., Sheng, G. & Luo, X. Amperometric sensing of nitrite using a glassy carbon electrode modified with a multilayer consisting of carboxylated nanocrystalline cellulose and poly(diallyldimethyl ammonium) ions in a PEDOT host. Microchim Acta 183, 2031–2037 (2016).

  15. 15.

    Gokhale, A. A., Lu, J., Weerasiri, R. R., Yu, J. & Lee, I. Amperometric detection and quantification of nitrate ions using a highly sensitive nanostructured membrane electrocodeposited biosensor array. Electroanalysis 27, 1127–1137 (2015).

  16. 16.

    Moorcroft, M. J., Davis, J. & Compton, R. G. Detection and determination of nitrate and nitrite: a review. Talanta 54, 785–803 (2001).

  17. 17.

    Siontorou, C. G. & Georgopoulos, K. N. A biosensor platform for soil management: the case of nitrites. J Clean Prod 111(Part A), 133–142 (2016).

  18. 18.

    Zahedi, M. M., Amiri, A. H. & Nasiri, M. Spectrophotometric monitoring of nitrite in seawater after liquid microextraction of its derivative with 2, 3-diaminonaphthalene. Water Qual Res J 52, 11–17 (2017).

  19. 19.

    Schierenbeck T. M. & Smith M. C. A path to impact for autonomous field deployable chemical sensors: A case study of in situ nitrite sensors. Env Sci Technol (2017).

  20. 20.

    Yokota, M., Okada, T. & Yamaguchi, I. An optical sensor for analysis of soil nutrients by using LED light sources. Meas Sci Technol 18, 2197 (2007).

  21. 21.

    Pandey, G., Kumar, R. & Weber, R. J. Real Time Detection of Soil Moisture and Nitrates Using On-Board In-Situ Impedance Spectroscopy. 2013 IEEE International Conference on Systems, Man, and Cybernetics; 2013 13–16 Oct. 2013; 2013. p. 1081–1086.

  22. 22.

    Ali, M. A. et al. Microfluidic impedimetric sensor for soil nitrate detection using graphene oxide and conductive nanofibers enabled sensing interface. Sensor Actuat B 239, 1289–1299 (2017).

  23. 23.

    Davis F., Collyer S. D. & Higson S. P. The construction and operation of anion sensors: current status and future perspectives. Anion Sensing 97–124 (2005).

  24. 24.

    Hutchins, R. S. & Bachas, L. G. Nitrate-selective electrode developed by electrochemically mediated imprinting/doping of polypyrrole. Anal Chem 67, 1654–1660 (1995).

  25. 25.

    Wasim, F., Mahmood, T. & Ayub, K. An accurate cost effective DFT approach to study the sensing behaviour of polypyrrole towards nitrate ions in gas and aqueous phases. Phys Chem Chem Phys 18, 19236–19247 (2016).

  26. 26.

    Bomar, E. M., Owens, G. S. & Murray, G. M. Nitrate Ion Selective Electrode Based on Ion Imprinted Poly (N-methylpyrrole). Chemosensors 5, 2 (2017).

  27. 27.

    Brooke R. et al. Recent advances in the synthesis of conducting polymers from the vapour phase. Prog Mater Sci (2017).

  28. 28.

    Brooke, R. et al. Inkjet printing and vapor phase polymerization: patterned conductive PEDOT for electronic applications. J Mater Chem C 1, 3353–3358 (2013).

  29. 29.

    Ramaswami, S., Gulyas, H., Behrendt, J. & Otterpohl, R. Measuring nitrate concentration in wastewaters with high chloride content. Int J Env Anal Chem 97, 56–70 (2017).

  30. 30.

    Badea, M. et al. New electrochemical sensors for detection of nitrites and nitrates. J Electroanal Chem 509, 66–72 (2001).

  31. 31.

    Le Goff, T. et al. An accurate and stable nitrate-selective electrode for the in situ determination of nitrate in agricultural drainage waters. Analyst 127, 507–511 (2002).

  32. 32.

    Dawson, R. E. et al. Experimental evidence for the functional relevance of anion–π interactions. Nature Chem 2, 533–538 (2010).

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Authors thank John Gouzos from CSIRO Land and Water division for undertaking the IC measurements. The authors from Sentek Pty Ltd would like to acknowledge the support of the Australian Government through the Department of Industry, Innovation and Science’s Innovation Connections Grant (RC53575). DRE acknowledges the support of the Australian Research Council through the Future Fellowship scheme (FT160100300).

Author information


  1. Future Industries Institute, University of South Australia, Mawson Lakes, South Australia, 5095, Australia

    • Sam Rudd
    •  & Drew Evans
  2. Sentek Pty Ltd, Stepney, South Australia, 5069, Australia

    • Michael Dalton
    • , Peter Buss
    • , Amanda Treijs
    • , Michael Portmann
    •  & Nick Ktoris


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S.R., M.D., P.B., A.T., M.P., N.K. and D.E. contributed to the experimental work and the writing of the main manuscript text. D.R. and S.R. prepared Figs 1–3. All authors reviewed the manuscript.

Competing Interests

MD, PB, AT, MP and NK are employees of Sentek Pty Ltd, a private company who manufacture and sell environmental sensing equipment. As such they declare their competing financial interest in relation to the described work. DR and SR declare no potential conflict of interest.

Corresponding author

Correspondence to Drew Evans.

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