A major challenge in the industrial use of enzymes is maintaining their stability at elevated temperatures and in harsh organic solvents. In order to address this issue, we investigated the use of nanotubes as a support material for the immobilization and stabilization of enzymes in this work. SnO2 hollow nanotubes with a high surface area were synthesized by electrospinning the SnCl2 precursor and polyvinylpyrrolidone (dissolved in dimethyl formamide and ethanol). The electrospun product was used for the covalent immobilization of enzymes such as lipase, horseradish peroxidase, and glucose oxidase. The use of SnO2 hollow nanotubes as a support was promising for all immobilized enzymes, with lipase having the highest protein loading value of 217 mg/g, immobilization yield of 93%, and immobilization efficiency of 89%. The immobilized enzymes were fully characterized by various analytical methods. The covalently bonded lipase showed a half-life value of 4.5 h at 70 °C and retained ~91% of its original activity even after 10 repetitive cycles of use. Thus, the SnO2 hollow nanotubes with their high surface area are promising as a support material for the immobilization of enzymes, leading to improved thermal stability and a higher residual activity of the immobilized enzyme under harsh solvent conditions, as compared to the free enzyme.
Owing to the benignity, high selectivity, and good activity of biocatalysts, there has been a global race to industrialize enzymes for the modern world. Nanoparticle-based enzyme immobilization has attracted significant attention in the past few decades for industrial applications. Several types of nanoparticles with distinct structural and morphological features (e.g., nanotubes, yolk shell, spherical, hollow, composite, and pore shell nanoparticles) have been used for the immobilization of enzymes, prolonging the availability of the biocatalysts and resulting in redundant downstream and purification processes1,2,3,4,5. Numerous benefits such as improvements in the activity, stability, and reusability of the enzyme can be achieved by their immobilization onto porous nanostructures. However, in most cases, the use of nanoparticles results in decreased enzyme activity and lesser loading. Thus, immobilization techniques such as adsorption, entrapment, encapsulation, and covalent immobilization display a wide variety of behaviors in terms of the activity, selectivity, specificity, and stability of the enzyme6,7,8.
Glucose oxidase (GOx), horseradish peroxidase (HRP), and lipases have numerous industrial applications, including in food preservation, baking, wine production, development of biosensors9, medicines, bioremediation, degradation of dyes10, pharmaceuticals, leather making, paper production, foods, cosmetics, and biodiesel production11,12,13. Apart from ester synthesis, lipases also play a pivotal role in acidolysis, alcoholysis, hydrolysis, aminolysis, and interesterification reactions13. Although they are efficient biocatalysts, use of pure enzymes is associated with several problems such as their sensitivity, limited reusability, cost, and stability, which limit their industrialization14,15,16. To overcome these problems, enzymes have been immobilized onto a variety of supports, including bio-related nanoarchitectures, metal-organic frameworks and hybrids, polymers, and nanomaterials17,18,19,20,21,22,23. Additionally, several immobilization strategies have been developed to increase the stability and reusability of enzymes6,24. After achieving an efficient enzyme immobilization, researchers have sought to improve the various properties of the enzymes and nanoparticles to develop an ideal biocatalyst8,25.
SnO2 nanoparticles have been previously synthesized by solvothermal, hydrothermal, gel combustion, spray pyrolysis, physical vapor deposition, and sol-gel methods26,27,28. Compared to the SnO2 nanoparticles synthesized by the above mentioned methods, SnO2 nanotubes prepared by the simple, short, and economical electrospinning method have a well-defined tubular structure, with the lumen opening to the exterior. Additionally, the internal morphology and hydrophobicity of the support significantly affect the activity, stability, specificity, and selectivity of the immobilized enzyme29,30. Moreover, SnO2 nanoparticles exhibit different levels of electrical, optical, and magnetic properties depending on their particle size31,32. A variety of nanotubes and their composites, including carbon, halloysite, and SnO2 nanoparticles have previously been used as supports for the immobilization of enzymes17,33,34,35,36. Therefore, in the present study, unique SnO2 hollow nanotubes were synthesized by electrospinning methods and used for the first time as supports for the immobilization of enzymes, including lipase, HRP, and GOx. Compared with other nanoparticles reported previously17,20,36,37,38,39,40, the synthesized SnO2 hollow nanotubes were found to be superior supports for immobilizing a variety of enzymes owing to their large surface areas, additional surface porosities, and luminal openings. The improved immobilization yield and reusability of the SnO2 nanotube-bound enzymes indicate that these hollow nanotubes are promising supports for enzyme immobilization. In addition, enzymes immobilized on SnO2 hollow nanotubes exhibited higher loadings and improved properties compared to enzymes immobilized on carbon nanotubes35,38,39. Given the improved biochemical properties and stability of the immobilized enzymes, it is reasonable to expect that the SnO2 hollow nanotubes would also be a promising candidate for the immobilization of various important enzymes for industrial applications.
Results and Discussion
Synthesis and characterization of the SnO2 hollow nanotubes
A schematic diagram of the synthesis of SnO2 hollow nanotubes and the subsequent immobilization of the enzyme is given in Fig. 1. The SnO2 hollow nanotubes were prepared by a modified version of a previously reported method41. A solution of polyvinylpyrrolidone (PVP, used as the sacrificial agent to form the main pores of the nanotubes) and SnCl2 in a mixed solvent solution of dimethyl formamide (DMF) and ethanol was electrospun. The product was dried in an oven and then subjected to thermal annealing in air. This calcination process resulted in the successful fabrication of SnO2 hollow nanotubes of length ranging from a few hundred nanometers to a few micrometers and diameter in the range of 200–300 nm. The SnO2 hollow nanotubes were composed of clusters of tens of nanometer-sized nanoparticles (Fig. 2). Although the tubular structures could be inferred from the scanning electron microscopy (SEM) images (Fig. 2a and c), transmission electron microscopy (TEM) (Fig. 2e) and the back-scattering mode of SEM were used to confirm the inner vacancy of the structures (Supplementary Fig. 1). The tubular shape instead of a rod-like form indicated that the structure consisted of SnO2 nanoparticles, which were ~40–50 nm in size (Fig. 2e). To gain further insight into the crystallographic attributes of the SnO2 nanotubes, X-ray diffraction (XRD) was performed (Fig. 3a). All the peaks of the XRD pattern could be assigned to the rutile phase of SnO2 (JCPDS No. 41–1445). Furthermore, the size of the SnO2 nanoparticles was calculated from the strongest peak (110) using Scherrer’s equation and found to be 43.18 nm, which matched the size evaluated from the SEM images (Fig. 2a and c). The surface area and total pore volume of the SnO2 hollow nanotubes were 15.6 m2/g and 0.12 cm3/g, respectively, as calculated with the multi-point Brunauer–Emmett–Teller (BET) method, and the isotherm was plotted accordingly (Fig. 3b). The surface area and total pore size significantly decreased up to 6.3 m2/g and 0.05 cm3/g, respectively, upon immobilization of the enzyme on the SnO2 hollow nanotubes, indicating both internal and external immobilization. Moreover, as revealed in the Barrett–Joyner–Halenda (BJH) plot (Supplementary Fig. 2), there were small peaks corresponding to the pore sizes of 24.4 and 58.1 nm, indicating the existence of pores among the SnO2 nanoparticles. A decrease in the SnO2 hollow nanotube porosity to 21.3 and 43.6 nm indicated internal immobilization of the enzyme, which was expected to facilitate the diffusion of the substrate. The presence of larger pores (with diameter over 100 nm) was attributed to the hollow structure of the SnO2 nanotubes. Additionally, the average pore diameter deduced from the BJH method for the SnO2 nanotubes was 45.4 nm. As globular enzymes like lipase, HRP, and GOx have a typical diameter of 3–6 nm, they could be easily loaded into the lumen of these SnO2 nanotubes and it was difficult for them to diffuse back out owing to their covalent binding with the support matrix. The use of SnO2 nanotubes as a support resulted in a high loading (217 mg/g) and immobilization yield (93%) of lipase, which could be attributed to the additional porosity along with the luminal openings (evident in Fig. 3b and Supplementary Fig. 2). In comparison with a previous report that indicated an enzyme loading of 11.6 mg/g for pristine halloysite nanotubes (HNTs) and 168.8 mg/g for dopamine surface-modified HNTs, the highly porous SnO2 hollow nanotubes synthesized in this work provide a better support for enzyme immobilization33.
The synthesized particles were functionalized with different concentrations of glutaraldehyde and the immobilization yield (%) was checked versus the immobilization efficiency (%). Glutaraldehyde concentrations of 0.8, 0.6, and 1 M were investigated for optimal functionalization of the SnO2 hollow nanotubes for lipase, HRP, and GOx immobilization, respectively. Overall, the immobilization of the enzymes increased over time, and the optimum immobilization efficiency and loading were obtained after 24 h for all three enzymes (Fig. 4a). The pH of the solution had a profound effect on enzyme immobilization. Among the solutions with different pH utilized for optimizing immobilization, the solution with pH 7.5 gave the highest immobilization efficiency and immobilization yield for lipase (Fig. 4b), whereas the optimum pH value for the immobilization of HRP and GOx was 7.0. Moreover, enzyme immobilization at 4 °C resulted in the highest immobilization efficiency and immobilization yield, as depicted in Supplementary Fig. 3 for lipase.
The SnO2 hollow nanotubes showed an immobilization yield of 93% and an immobilization efficiency of 89% for lipase. The activities of HRP and GOx were also retained to a substantial extent after immobilization, with immobilization efficiencies of 78% and 81%, respectively (Table 1). The variations in the immobilization parameters may be because of the differences in the properties of the enzymes. It was found that for each 1 g of SnO2 hollow nanotubes, the loading of lipase, HRP, and GOx was 217, 181, and 196 mg, respectively.
Leaching and cross-linking of immobilized lipase
Although immobilization can significantly improve the different characteristics of the enzymes, leaching is still a key problem because it hinders the repeated use of the immobilized enzyme4,42,43. The SnO2 hollow nanotube-immobilized lipase was treated with NaCl to evaluate the leaching effect. A substantial amount of the wash-out protein was detected in the supernatant. This excess amount of the enzyme in the supernatant corresponded to that had physically adsorbed onto the nanotubes, in addition to the amount that had covalently attached to the substrate. In order to solve the leaching problem and to improve the stability and reusability of the enzyme, additional cross-linking was achieved with glutaraldehyde44,45. Among the three enzymes, the least amount of leaching (11%) was noticed for lipase after its cross-linking with 0.1 M glutaraldehyde. The other two enzymes also showed a decrease in leaching after cross-linking (Supplementary Table 1). However, a significant decrease in the immobilization efficiency was observed on using higher concentrations of glutaraldehyde (0.2–0.5 M), which may be due to substrate diffusion limitations after the high cross-linking of the enzyme4.
Characterization of the immobilized lipase
Maximum residual activities of the free, immobilized, and cross-linked lipases were obtained at the pH values of 8.5, 9, and 9.5, respectively (Fig. 5a). The immobilized and cross-linked lipases maintained higher residual activities over a broad pH range of 8.5–10 in comparison with the free lipase. The residual activities of the cross-linked lipase were 100%, 98%, and 93% at pH 9, 9.5, and 10, respectively, whereas those of the free enzyme were 87%, 72%, and 59%, respectively. In addition to the shift in the optimum pH values after immobilization, the residual activities of the immobilized and cross-linked lipases were also improved at higher temperatures. The optimum temperature for the free lipase was 50 °C, which shifted to 55 °C for the immobilized and cross-linked lipases (Fig. 5b). At higher temperatures (60–80 °C), the cross-linked lipase retained a higher relative activity in comparison to the free and immobilized lipases. This shift in optimum temperature for the immobilized and cross-linked lipases may be attributed to their binding behavior. The SnO2 hollow nanotubes provide additional rigidity to the external backbone of the immobilized enzyme after firmly holding it within their lumen (Fig. 2f). Consequently, the effects usually appeared at higher temperatures (e.g., the breakdown of the interactions responsible for the proper globular and catalytically active structure of the enzyme) became less prominent, leading to enhanced thermal stability of the immobilized enzyme46.
Instrumental analyses of the immobilized enzyme
The SEM images of the immobilized lipases are presented in Fig. 2. It is evident that the immobilized enzyme is bound to the SnO2 hollow nanotubes (Fig. 2b and d). The TEM images of the immobilized lipases are provided in Fig. 2e and f. The dark internal background of the SnO2 hollow nanotubes after enzyme immobilization confirms the internal localization of lipase. These results suggest that lipase was bound both on the surface and in the internal lumen of the SnO2 hollow nanotubes, owing to the porous nature of the support (Fig. 2b,d and f). In order to explore and quantify the SnO2 hollow nanotube modifications before and after enzyme immobilization, elemental analysis was performed (Supplementary Table 2). A significant increase in the relative content of each element (C, O, and N) after enzyme immobilization indicated a high loading of the enzyme onto the SnO2 hollow nanotubes. Moreover, the high intensity of the green fluorescence on the surface of the SnO2 hollow nanotubes was a result of the fluorescein isothiocyanate (FITC)-tagged lipase, indicating a greater concentration of the enzyme on the surface (Fig. 6). A uniform distribution of the enzyme along the walls of the nanotubes was indicated by the green fluorescence (Fig. 6b and c). Thus, on the basis of the SEM, TEM, and confocal laser scanning microscopy (CLSM) results, it was concluded that the enzyme had been immobilized both internally and externally onto the SnO2 hollow nanotubes. In addition, the high loading of lipase was confirmed, with a significant reduction in weight to 59% observed for the SnO2 hollow nanotubes-lipase complex in the thermogravimetric analysis (Supplementary Fig. 4). The presence of additional peaks at 1718–1613 cm−1 in the Fourier-transform infrared (FTIR) spectrum of the immobilized lipase, which could be correlated to the amide bond (N=C=O) stretching vibrations, further illustrated the efficient immobilization of the enzyme (Fig. 6a). The O–Sn–O stretching vibrations of the SnO2 nanotube particles were detected at 637 cm−1 47,48,49. The peaks in the range of 1400–757 cm−1 in the FTIR spectrum of SnO2-bound lipase were attributed to the C–O, C–N, and C–C vibrations. Broad bands detected at 1550–1650 cm−1 are consistent with the C=O stretching (amide I band, 1650 cm−1) and N-H bending (amide II band, 1550 cm−1) vibrations of the protein, with the additional C=O stretching peak (1730 cm−1) confirming lipase immobilization50. Moreover, the peaks between 3050 and 3500 cm−1 could be assigned to the –OH vibrations of amino acids51. The secondary structure of lipase immobilized on SnO2 hollow nanotubes was examined by circular dichroism (CD). The immobilized lipase exhibited a similar CD spectrum to its free form, suggesting that no significant change to its secondary structure occurs upon immobilization (Supplementary Fig. 5).
The kinetic parameters (K m and V max) of free lipase changed after its immobilization onto the SnO2 hollow nanotubes (Table 2). The Michaelis–Menten model was applied for the determination of these parameters. The apparent K m and V max values for free lipase were 0.71 mM and 217 µmol·min−1·mL−1, respectively. After immobilization, a slight increase of 6.6% and 12.4% in the K m value, and a decrease of 11.2% and 16.6% in the V max value, were noticed for the immobilized and cross-linked lipases, respectively. This increase in K m and decrease in V max after immobilization onto the SnO2 hollow nanotubes is indicative of substrate inhibition or diffusional hindrances. After enzyme immobilization, most of the nanoparticle surface, lumen, and pores are occupied by the enzyme, which provides a low surface area for substrate interaction. The opposite phenomenon (i.e., a decrease in K m and an increase in V max) appeared when the enzyme loading was decreased to half of the original loading (Supplementary Table 3). This further supports the rationale that the porosity and nanoparticle surface area would decrease with heavy enzyme loading.
Effect of metal ions and organic solvents
The effect of the presence of different metal ions in 2 mM concentration on the activity of the free and immobilized lipases was investigated. Divalent ions, especially Ca2+ and Mn2+, increased the activity of the immobilized lipase by 62% and 45%, respectively. Although the use of most of the other metal ions led to similar residual activities, addition of Na+ and K+ ions resulted in a sharp decrease in the activity of the free enzyme (Supplementary Table 4). Using solvents such as dimethyl sulfoxide (DMSO), acetone, n-hexane, and DMF resulted in a significant increase of 168%, 152%, 144%, and 117%, respectively, in the immobilized lipase activity. In contrast, phenol, methyl formate, acetic acid, and sodium dodecyl sulfate reduced the activity of the enzyme. An increase in the relative activity (%) was recorded for nonpolar hydrophobic solvents, and vice versa for polar hydrophilic solvents (Supplementary Table 5).
Thermal, pH stabilities and reusability of the enzyme
The thermal stability of an enzyme is of utmost importance for an evaluation of its industrial applicability. Weak forces of attraction between the enzyme and the support particle typically result in poor operational stability, and therefore, covalent immobilization has been introduced to achieve a more firm binding and better stability34,52. The stabilities of the free, immobilized, and cross-linked lipases at different temperatures (35–70 °C) were recorded after 4.5 h (Fig. 7a). It was found that the stability of the immobilized and cross-linked lipases at 70 °C increased from 4% (free lipase) to 42% and 61%, respectively, after incubation for 4.5 h, which was almost 11- and 15-fold higher than that of free lipase (Supplementary Fig. 6). The half-lives of the free, immobilized, and cross-linked lipases at 50 °C were 7.1, 22, and 31 h, respectively (Supplementary Fig. 6). The half-life of the cross-linked lipase was 141% and 333% higher than that of the immobilized and free lipases, respectively. Overall, the thermal stability of the cross-linked lipase was significantly enhanced after cross-linking with glutaraldehyde. Compared with the results of a previous report, in which a Fe3O4@chitosan-bound lipase retained 53% of its initial activity after 3 h incubation at 70 °C, the SnO2 hollow nanotube-immobilized lipase showed 150% higher relative stability at the same temperature53. In comparison with yet another study where a hen egg derivative-immobilized lipase showed a half-life of 2 h at 70 °C, the immobilized lipase in our study presented 225% higher relative stability at the same temperature4,45. The enhanced thermostability of the immobilized and cross-linked lipases described in this work may be accredited to their binding mode with the SnO2 hollow nanotubes, in which the carrier provided an external backbone to the enzyme to avoid its structural deformation and thus the negative effects of temperature were minimal (Figs 2f and 3b). In addition to its thermal stability, the immobilized lipase showed a significant increase in its pH stability. The immobilized and cross-linked lipases showed 38% and 52% stability at pH 11 after 4 h, whereas the free enzyme completely lost its residual activity (Supplementary Fig. 7). At an alkaline pH, the immobilized and cross-linked lipases were more stable compared to the free lipase. This increase in pH stability may be attributed to the binding behavior of the lipase with the SnO2 hollow nanotubes that maintains the three-dimensional structure of the enzyme even at elevated pH values and resists the interference of high pH54.
The reusability of an enzyme also plays a pivotal role in its industrial application. As indicated in Fig. 7b, the cross-linked and immobilized lipases retained 91% and 82% of their original activities after ten consecutive cycles of use, where the cross-linked lipase had better performance in this regard. The loss of activity of the cross-linked and immobilized lipases can be attributed to the weakening of the binding between the enzyme and the support. Moreover, frequent encounters between the active site and the substrate may distort the immobilized enzyme, resulting in its decreased catalytic efficiency. In previous studies, the reported loss in residual activity of immobilized lipase was 12% after the first cycle35, 55% after seven cycles45, and 30% after ten repetitive cycles53.
The cyclic voltammograms of the SnO2 hollow nanotubes and the free and immobilized lipases affixed onto a glassy carbon electrode (GCE) in a buffer (50 mM) containing palm oil (50 mg/dL), are presented in Supplementary Fig. 8. The anodic and cathodic current peaks for palm oil were detected in the presence of a tri-electrode biosensor. The bioelectrode showed reduction potentials of 0.49, 0.58, and 0.71 V for the free SnO2 hollow nanotubes, free lipase, and SnO2 hollow nanotube-immobilized lipase, respectively. Here, the shift in the redox potential of palm oil for the immobilized lipase might be primarily due to the charge-conducting SnO2 hollow nanotubes. The maximum peak current densities for the free and immobilized lipases in the presence of 50 mg/dL of palm oil were 0.22 and 1.54 µA cm−2, respectively. With these highly desirable electrochemical properties, the results suggest that the immobilized lipase is quite efficient at detecting long-chain fatty acids like palm oil. The high recognition ability of the lipase toward long-chain fatty acids after its immobilization onto SnO2 nanotubes may be attributed to the conformational changes in the enzyme structure that lead to lid domain opening, which facilitates enzyme–substrate interactions. The optimum pH values for the maximum anodic currents of the free and immobilized lipases fixed onto the GCE were pH 8.0 and 8.5, respectively. The current density increased linearly with an increase in the substrate concentration from 25 to 100 mg/dL. Here, the immobilized lipase was more efficient for the detection of palm oil compared to the free enzyme. Thus, this work presents the first example of an enzyme immobilized onto SnO2 hollow nanotubes and its use as a biosensor.
In this study, SnO2 hollow nanotubes with a high surface area were prepared by the electrospinning method. The unique properties of the SnO2 hollow nanotubes were employed as a support for the immobilization of lipase, GOx, and HRP. Based on the higher loading capacities and residual activities of the enzymes after their immobilization, it is concluded that SnO2 hollow nanotubes are an efficient support for enzyme immobilization. Moreover, cross-linking significantly enhanced the properties of the enzymes, such as their stability at elevated temperatures. Additionally, the high stability of the SnO2 hollow nanotube-immobilized enzymes, both in commonly used solvents and at high pH values, makes them a suitable alternative biocatalyst for a broad range of applications. Therefore, the immobilization of important enzymes on SnO2 hollow nanotubes (with enhanced electrochemical properties) as a support is a promising approach for numerous industrial applications.
Materials and reagents
Lipase from Thermomyces lanuginosus (Lipozyme TL 100 L) was procured from Novozymes Inc. (Franklinton, NC, USA). HRP and GOx were obtained from Sigma-Aldrich (St. Louis, MO, USA). p-Nitrophenyl palmitate (p-NPP), pyrogallol, glucose, o-dianisidine, hydrogen peroxide, Triton X-100, DMSO, DMF, acetone, and acetic acid were also purchased from Sigma-Aldrich. All other materials were of analytical grade and obtained from commercial sources, unless otherwise stated.
Synthesis of SnO2 hollow nanotubes
The SnO2 hollow nanotubes were prepared by following a modified version of a previously reported method41. A specific amount of SnCl2 (0.4 g) was dissolved in a 1:1 solvent mixture of DMF (2.5 mL) and ethanol (2.5 mL). After the solution had become homogeneous, 0.5 g of PVP (M W = ~130 M) was dissolved in the above solution at 50 °C for 3 h. The solution was then loaded into a syringe and electrospun at 0.5 mL/h at 14–15 kV. The collected electrospun product was dried overnight in an oven at 120 °C to evaporate any residual solvent. Finally, the product was thermally annealed in air at 500 °C for 1 h to remove the PVP and to form SnO2. The as-prepared SnO2 hollow nanotubes were characterized by SEM, TEM, XRD, and BET analyses.
The lipase activity was measured using p-NPP as the substrate, according to a previously described method42. The assay was performed at 50 °C in a total reaction volume of 200 µL that contained 176 µL of the Tris-HCl buffer (20 mM, pH 8.5) and 20 µL of p-NPP. The activity of HRP was determined by using hydrogen peroxide (0.97 M) and pyrogallol (13 mM) as substrates in sodium phosphate buffer (0.1 M) at pH 6. The GOx assay was performed with a HRP (20 µg/mL)-coupled reaction, using o-dianisidine (0.32 mM) and glucose (0.1 M) as the substrates in phosphate buffer (0.24 M) at pH 5.643.
For the immobilization of enzymes onto the SnO2 hollow nanotubes, 10 mg of the nanoparticles were functionalized with 0.6 M glutaraldehyde. The nanoparticles were then mixed with 1 mg of the enzyme (GOx, HRP, or lipase) at different pH and incubated at 4 °C and 150 rpm for 24 h. The unbound enzyme molecules were separated into the supernatant by centrifugation at 13000 rpm for 10 min at 4 °C. The concentration of proteins in the supernatant was measured by the Bradford assay with bovine serum albumin as the standard55. The immobilization yield and immobilization efficiency were calculated as described by Patel et al.56. The synthesized SnO2 hollow nanotubes not only provided a profound surface area for the immobilization of the enzyme but also increased the stability and activity of the enzyme under various harsh conditions.
Optimization of the incubation time
To determine the optimal duration for an efficient immobilization of enzymes onto the SnO2 hollow nanotubes, the amount of enzyme immobilized on the particles was determined at specific time intervals. Different concentrations of the enzyme (10–400 mg of enzyme per gram of support) were used to determine the loading capacity of the SnO2 hollow nanotubes in the reaction. As lipase demonstrated the highest immobilization efficiency and immobilization yield, it was selected for further study.
Leaching and cross-linking of enzymes
The leaching of immobilized enzymes on pre-functionalized SnO2 hollow nanotubes was determined under similar conditions by treatment with NaCl (1 M) for 1 h at 25 °C. The detached enzymes were monitored by analyzing the protein concentration in the supernatant after centrifugation at 10000 rpm for 10 min at 4 °C. The extent of leaching was determined as described by Patel et al.56. The immobilized enzymes were cross-linked, using glutaraldehyde (0.1 M) in phosphate buffer (50 mM, pH 7), for 30 min at 4 °C with shaking at 150 rpm.
The surface structures of the SnO2 hollow nanotubes were characterized by SEM (SUPRA 55VP; Carl Zeiss, Oberkochen, Germany). For the TEM measurements, the dried films were first immersed in ethanol and a drop of this solution was placed onto a standard copper grid. The solvent was then allowed to evaporate in air. The XRD experiment was performed on a Rigaku 18 kW rotating anode X-ray generator operated at 40 kV and 300 mA. The measurements were performed using Cu Kα radiation (λ = 1.5406 Å) in the 2θ range of 10°–80° with a scanning speed of 3°/min, and the distance between the sample and the detector was 185 mm. The specific surface area of the SnO2 hollow nanotubes was determined from the N2 adsorption–desorption isotherm by the BET method (for specific surface area) using a Belsorp-mini II analyzer after drying the sample at room temperature for a day in a vacuum oven. Prior to the measurements, the SnO2 hollow nanotubes were degassed at 70 °C under dynamic vacuum (10−2 Torr) for 1 h. The total carbon, nitrogen, and oxygen contents of the SnO2 hollow nanotubes before and after lipase immobilization were analyzed using the 2400 Series II CHNS/O Elemental Analyzer (100 V; PerkinElmer, Waltham, MA, USA). Both free and immobilized enzymes were characterized by FTIR spectroscopy. In the CLSM analysis, DMSO (2 mg/mL) was used for the dissolution of 8 mg of FITC to label 1 mL of lipase in carbonate buffer (0.5 M, pH 9.0) and the resulting solution was incubated at 300 rpm for 6 h in the dark. The excess FITC was removed by dialysis against distilled water. The FITC-labeled lipase was then immobilized onto the SnO2 hollow nanotubes. CLSM images were taken with an FV-1000 Olympus confocal microscope. The organic matter attached to the SnO2 hollow nanotubes was detected by thermogravimetric analysis. CD analysis was performed using a CD detector (Chirascan-plus, Applied Photophysics, UK).
Characterization of the immobilized enzyme
The effect of pH on the activity of the free, immobilized, and cross-linked lipases was determined by using Tris-HCl buffer at different pH. Moreover, the effect of temperature on the activity of each type of lipase was determined at 35–80 °C. Similarly, the effects of different metal ions, organic solvents, and surfactants on both free and immobilized enzyme activities were determined.
For determination of the kinetic parameters, the sample was incubated at the optimum temperature and pH with different concentrations of the substrate (0.05–5.0 mM). The kinetic parameters K m and V max were calculated by nonlinear regression fitting of the Michaelis–Menten equation using Prism 5 (GraphPad Software Inc., La Jolla, CA, USA).
Stability of free and immobilized enzymes
To investigate the thermal stability of the free, immobilized, and cross-linked lipases, the residual activity (%) of each enzyme was calculated after 4.5 h of incubation at different temperatures (35–70 °C) at pH 7.0. For this purpose, the samples were taken at every 30-min interval and the residual activity (%) was measured under standard assay conditions. The initial activity was taken as 100% in each case. Additionally, the stability of the free, immobilized, and cross-linked lipases was investigated by calculating the residual activity in each case after incubation for 4 h at different pH at the optimal temperature.
Reusability of the immobilized enzyme
The reusability of the enzyme was determined for both immobilized and cross-linked lipases under standard conditions. After each cycle, the enzyme-bonded nanoparticles were separated by centrifugation at 13000 rpm for 5 min and 4 °C, washed twice with the buffer, and then resuspended in the fresh buffer/substrate solution under standard assay conditions. The residual activity (%) in the first cycle was taken as 100%.
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This research was supported by the Ministry of Science, ICT and Future Planning, Republic of Korea (2013M3A6A8073184, NRF-2017R1A2B3011676, NRF-2017R1A4A1014806, NRF-2015R1C1A1A01053807). This work also was supported by the Energy Efficiency & Resources Core Technology Program of the KETEP, granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (20153010092130). This work was also supported by the 2015 KU Brain Pool Fellowship of Konkuk University. This research was supported by the KU Research Professor program of Konkuk University.
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Indian Journal of Microbiology (2018)