Aggregation of amyloid β (Aβ) peptides is a significant event that underpins Alzheimer disease (AD) pathology. Aβ aggregates, especially the low-molecular weight oligomers, are the primary toxic agents in AD and hence, there is increasing interest in understanding their formation and behavior. Aggregation is a nucleation-dependent process in which the pre-nucleation events are dominated by Aβ homotypic interactions. Dynamic flux and stochasticity during pre-nucleation renders the reactions susceptible to perturbations by other molecules. In this context, we investigate the heterotypic interactions between Aβ and fatty acids (FAs) by two independent tool-sets such as reduced order modelling (ROM) and ensemble kinetic simulation (EKS). We observe that FAs influence Aβ dynamics distinctively in three broadly-defined FA concentration regimes containing non-micellar, pseudo-micellar or micellar phases. While the non-micellar phase promotes on-pathway fibrils, pseudo-micellar and micellar phases promote predominantly off-pathway oligomers, albeit via subtly different mechanisms. Importantly off-pathway oligomers saturate within a limited molecular size, and likely with a different overall conformation than those formed along the on-pathway, suggesting the generation of distinct conformeric strains of Aβ, which may have profound phenotypic outcomes. Our results validate previous experimental observations and provide insights into potential influence of biological interfaces in modulating Aβ aggregation pathways.
Aggregation of Aβ constitutes the central process in Alzheimer disease (AD) pathology. Brains of AD patients contain large amounts of proteinacious plaques mainly comprised of insoluble Aβ fibril deposits. Monomeric Aβ peptides (Aβ40 and Aβ42) spontaneously undergo aggregation towards large fibrils in a nucleation dependent manner. The effects of nucleation on aggregation dynamics have been extensively studied1,2,3,4,5,6,7,8 that point to a key rate-limiting step for the formation of nucleus/nuclei9,10,11,12,13. Therefore, the dynamics associated with reactions leading up to nucleation is critical for aggregation. Energetically, the pre-nucleation phase is nebulous with monomers and oligomers (dimers, trimers etc.) involved in a dynamic flux dominated by stochastic interactions14,15,16. Furthermore, intrinsic disorder and amphipathic nature of Aβ facilitate multiple phase transitions and heterogeneous interactions during nucleation, making the process particularly sensitive to environmental factors such as pH, ionic strength, temperature and other interacting partners17,18,19,20,21,22. This is significant because smaller, soluble aggregates have emerged as the primary neurotoxic agents responsible for memory loss in AD23,24,25,26. Furthermore, it is clear that oligomers may not be the obligatory intermediates to fibril formation, and that the oligomers can also be populated along alternate pathways of aggregation (off-pathways)22, 27,28,29,30,31,32. Off-pathway oligomers differ from those formed along the on-pathway resulting in multiple conformational variants with distinct biochemical and cellular properties. Given the conformational diversity among oligomers, it is imperative to determine the factors that affect dynamics involved in such oligomer formation to establish a framework of molecular mechanisms that better defines amyloid progression.
One class of biologically important interacting partners that affect Aβ pre-nucleation dynamics are the anionic surfactants such as fatty acids and lipids33,34,35,36,37,38,39. Lipids and fatty acids are profoundly important in physiological contexts as they are abundant in both brain tissues and CSF40, 41. The amphipathic Aβ peptide is known to have strong affinity for membranes and hence, these interactions may affect the pre-nucleation dynamics. Several reports also demonstrate the effects of phospholipids on Aβ aggregation42,43,44. Similarly, both polyunsaturated (PUFAs) and saturated fatty acids are also known to have significant effects on the AD brain45, 46. Kumar and co-workers have previously reported the generation of 4–5 mers and 12–24 mers from lauric acid along a non-fibril formation pathway22. More importantly, using carbon chain lengths of C9-C12 fatty acids (FAs), they established that below (non-micellar), near (pseudo-micellar) and above (micellar) their respective critical micelle concentrations (CMCs), FAs generate Aβ oligomers or fibrils via distinct pathways22. A schematic of the switching dynamics from on- to the off-pathway with increasing concentration of FAs is shown in Fig. 1. Here the bold horizontal arrow signifies increasing FA concentration while the vertical arrows conceptually depict the dynamical aggregation regimes of the system with the inclusion of monomeric Aβ. It can be observed that low FA concentration involves the non-micellar phase where only the on-pathway reactions are active; while high FA concentration (beyond the CMC) involves the micellar phase where only the off-pathway reactions are active (with on-pathway being completely switched off). The middle phase of the FA concentration arrow denotes the near-CMC (i.e., pseudo-micellar) phase and is the most interesting one; here, both on- and off-pathway reactions are active and the monomeric Aβ may form aggregated oligomers from both pathways simultaneously (as denoted by the arrows).
In this report, we sought to understand the dynamics of heterotypic interactions between Aβ and FAs and how phase transitions of FAs modulate Aβ aggregation. We first use a reduced order model (ROM) and a corresponding mathematical analysis rooted in a linear stability argument to identify the switching behavior from on- to off-pathway in the presence of micellar interfaces. The ROM can be thought of as a toy-version of the far more complex biophysical problem. However, despite considering only 4-species in this model, the ROM still deals with a highly non-linear problem which captures the overall aggregation dynamics of the Aβ system and allows for rigorous examination of features such as dynamic stability which would be intractable in the case of a very fine grained model. Mathematical biologists consistently simplify complex biological problems to gain initial insight into problems, to understand the nature of equilibria, stability, bifurcations in the larger system, before venturing to speak of specific details which is the objective of the ROM. This analysis was used as the basis to further develop a more detailed model, which was built around the discoveries of the ROM and experimental data.
Secondly, using an ensemble kinetic simulation (EKS) model, we demonstrate that in three distinct phase-transition regimes, FAs significantly and consistently modulate Aβ aggregation by altering the pathways. These results are in agreement with the reported in vitro experimental observations, and provides detailed molecular insights into the heterotypic interactions between Aβ and FAs. These insights also reveal that generation of conformationally-distinct oligomeric strains (produced by the oligomers involved in the on- and off-pathways) are highly likely in physiological environments containing lipid and FA interfaces due to the dynamics involved in the heterotypic FA-Aβ interactions.
Stability analysis for a reduced order model (ROM)
We examined the sets of on- and off-pathway reactions shown below. The model system considered is as follows:
where the reaction 2 is referred to the on-pathway, while reaction 3 is the off-pathway. In these reactions, A 1 denotes the Aβ monomers while denotes the off-pathway monomers created in the presence of fatty acids, FA (considering critical micelle concentration, CMC). A n are the on-pathway oligomers while are the off-pathway oligomers made of n monomeric units. Finally, and are the forward and backward rate constants of the corresponding reactions (i = 1, 2, 3). The details of the modeling methodology are elaborated in the Methods section. We are aware that the on-pathway fibrils are considerably larger than the terminal species of the off-pathway, i.e. size of (A n ) ≫ size of (A ′ n ) or n ≫ n′. In the ROM analysis however, both pathways terminate at the same value of n since we believe this to be sufficient to elucidate the competition between the two pathways in our model. Since the only “bridge” from the on- to off-pathway is between the monomeric species, once the reaction chooses the path from , the on-pathway dominates in the steady state (or vice-versa) and higher order oligomer dynamics do not reveal anything more about the process. The asymmetric nature of the two pathways is however, considered in our follow-up analysis to understand detailed mechanistic phenomena.
Based on the results of our analysis presented in the Methods section, Fig. 2 shows the eigenvalues λ i (i = 1–4), of the Jacobian matrix for the mass action kinetic equations (18–21). These eigenvalues appear as a function of α 3, a normalized parameter which depend on the CMC and rate constants. The eigenvalues depict the rate of decay of any perturbation to the stable, non-zero equilibria of the system of equations (18–21). It so happens that the critical parameter α 3 is key to determining whether the on-pathway or off-pathway is dominant. The Fig. 2 shows some significant events in the dynamics of this system. As α 3 is increased from zero, the real part of the eigenvalues λ i are all negative, indicating that the equilibria are stable. When α 3 < 1.0, we observe that λ 2 < λ 1. However, when α 3 > 1.0, it follows that λ 2 > λ 1 indicating an exchange in the magnitude of the stability. For α 3 > 1.0, the species B 1 (dimensionless version of A 1) dominate in magnitude and also become more stable than (dimensionless version of ), indicating a transcritical-like bifurcation at α 3 = 1.0.
Note that in the dynamical systems literature, transcritical bifurcation refers to a stable equilibrium and an unstable equilibrium which exchange stabilities with respect to some parameter47. In our model system, the switching of stabilities is revealed by α 3, which, by its definition, contains the ratio of the forward to backward rate constants for this reaction, as well as the fatty acid concentration which enhances the formation of the off-pathway species. Figure 3(b) shows the rate of reformation of the species B n and , which is slower than the monomeric species.
Another interesting phenomenon that occurs in this system is a discontinuity in the eigenvalues or second switching which occurs at around α 3 = 1.87 (see Fig. 3(a)). The Table 1 in the Supplementary Information captures the variety of switching behaviors and changes in behavior of the ROM system when different parameters (rate constants) are varied. At this stage, the biophysical implication of this second critical point is unclear to us although it is most certainly a symptom of the high nonlinearity of the system.
We explore the effect of n on our system, for 1 ≤ n ≤ 20. Our analysis reveals significant changes to the stability of the system as n varies; in particular, the eigenvalues λ 1 and λ 2 show some dramatic changes with n. For 1 ≤ n < 12, multiple switching points (at least two) were observed with λ 2 eventually being more negative than λ 1, i.e. off-pathway eventually becomes more stable for sufficiently large α 3. However, for 12 ≤ n ≤ 20, the on-pathway eventually becomes more stable. The physical significance of the multiple switching, in particular the one beyond α 3 = 1 still eludes us and it remains to be verified if this an indication of some underlying bifurcation in the system or is simply an artifact of the nonlinear system or an indication of some nucleation type event observed in our previous work using similar methods48.
The results of our stability analysis with support from the Nash equilibrium for this system (see Methods section), allows us to draw the following conclusions: (i) The simplified, though highly nonlinear system of ROM equations with 4-species captures the essential dynamics of the Aβ system and can help explain the switching dynamics considering only the CMC; (ii) The non-dimensional parameter α 3 is seen to be the key control parameter in this analysis indicating that FA concentration, along with the ratio of the rate constants along this “bridge” reaction pathway determine the ultimate fate of the system. (iii) The presence of a second, discontinuous switching of eigenvalues at α 3 ≥ 1.87 potentially indicates a different biophysical phenomenon occurring along the off-pathway implicating the involvement of additional species that may play an important role in the FA dynamics. To explore the dynamics between on- and off-pathways of Aβ aggregation, we then conducted a more detailed ensemble simulation using chemical kinetics under varying concentration of FAs.
Ensemble kinetic simulation (EKSs) on Aβ-FA dynamics
In order to further investigate the dynamics between the on- and off-pathways in the presence of FAs, we used the EKS approach. EKS approach involves numerical ordinary differential equation (ODE)-based simulations that encompasses ensembles of the dynamic species involved in the reaction flux. Fundamental aspects of EKSs were used in the on-pathway model that we had previously reported10, 48. In this report, we have expanded the EKS model to simulate various phase-transition regimes of FAs and Aβ, especially along off-pathway (elaborated in the Methods section). Before modeling on- and off-pathway dynamics, it is imperative to simulate the phase-transitions of FAs during micelle formation. We specifically chose three concentration regimes at which FAs show distinct phase changes: low concentrations where the FAs are non-micellar (FA n ), near their CMCs where they are pseudo-micellar (FA pm ), and high concentration where they are fully micellar (FA m ). We conjecture the nomenclature, pseudo-micelles to define the dynamic state between a non-micelle and a micelle, which seemed to affect Aβ in a distinct way22. The hydrodynamic radii (Rh) estimates of FA pm and FA m indicated that the former is ~6–7 times larger than the latter, confirming the loosely-held, amorphous state of FA pm (Table 1).
Dynamics in non-micellar phase - Aβ–FA n interactions
First, for the control experiment in the absence of FAs (on-pathway reactions, exclusively), we find the forward rate constants during the nucleation stage (k nuon ) is considerably lower than the forward rate constant (k fbon ) of elongation stage, as expected (Fig. 4(a)). The simulated data showed good correlation with the experimental data with an R 2 value of 96.2, and an equally good agreement in the lag times (Fig. 4(a)) for C12, as well as C9-C11 FA data (Fig. 4(b)). The estimated rate constants for control, i.e., on-pathway, are shown in Supplementary Table 2 in Appendix-B under Supplementary Information. Secondly, in the presence of FA n , on-pathway reactions still dominate due to the absence of micelles (or pseudo-micelles) in the solution. We hypothesize that FA n s interact with Aβ and catalyze aggregation by altering the rate constants along the on-pathway by a factor of K, resulting in a change in the final fibril concentration. The corresponding set of reactions for this model are shown in Appendix-C under Supplementary Information. We obtain the value of K < 1 for the best fit with the experimental data pointing to a slowdown in the elongation stage of the on-pathway (Supplementary Table 3 in Appendix-C under Supplementary Information).
The simulations of Aβ interactions with C12 FA n when compared to the on-pathway fibril growth characteristics, provided two important observations. First, the overall aggregation was augmented with the introduction of FA n , consistent with the overall ThT intensity observed in experiments (Fig. 4(a); red). Second, the third phase of fibril growth curve (i.e., the slower growth phase towards saturation subsequent to the exponential growth phase) was delayed for a considerable amount of time with C12 FA n (as opposed to the on-pathway), despite no change in the lag time (Fig. 4(a)). We hypothesize these effects are caused by the introduction of FA n , which modulate the on-pathway rate constants. To test this hypothesis, we systematically analyzed the effects that FA n may have on the rates of on-pathway reactions at critical times of the aggregation process. First, the forward rate constants during the pre-nucleation stage was altered (increased/decreased) by a factor of K, which did not fit the fibril growth curve for the C12 FA n case and concomitantly could not validate this behavior (data not shown). Next, upon varying the forward elongation rate constant (k fbon ), a small decrease in the forward elongation rate constant render higher ThT intensity with delayed saturation time, and without changing the lag time. This suggests that the addition of FA n molecules effectively decrease the forward elongation rate constants. According to the Lee’s and our own on-pathway models48, 49, the elongation stage (ie. ; ; … and ) does not lead to the increase in ThT intensity directly as these reactions may not increase the number of ThT binding sites significantly to augment fluorescence intensities. Hence, a decrease in the elongation rate constant essentially extends the duration of the pre-nucleation stage, although without increasing the lag time. This subsequently could increase the formation of pre-nucleated on-pathway aggregates (specifically 12 mers, i.e., A 12s) resulting in higher ThT intensity/fibril growth. The increased number of A 12s resulting from these reactions then react with one another as well as the on-pathway oligomers during the pre-nucleation stage (monomers to 11 mers, i.e., A 1–A 11) to generate higher order aggregates with concomitant higher ThT intensities. The predicted K value for different initial FA n concentrations is shown in Supplementary Table 3 in Appendix-C under Supplementary Information. Similar corresponding plots for ThT vs simulations for C9-C11 FA n are shown in Fig. 4(b). The high R 2 values obtained for the fits (Supplementary Table 3) suggest that FA n s promote on-pathway fibrils by a slightly different mechanism as compared to the Aβ aggregation in the absence of FAs. Hence, as shown in Fig. 4(c), the ratio of /A 4 is effectively zero.
We further consider two hypothetical but physically relevant scenarios in which varying rates of entry of Aβ monomers and FAs were considered as opposed to a fixed concentration50. The reaction models for this scenario were then minimally modified to consider these additional entry rates as variables to evaluate the oligomer concentration dynamics. As expected in Fig. 4(c), the interaction of FA n with Aβ generated no off-pathway oligomers due to the absence of micelles or pseudo-micelles. Only on-pathway aggregates were formed exclusively, although by a slightly different mechanism. Based on these results, we determine that FA n s decrease the rates during the fibril elongation stage along the on-pathway, which seems to hold good for each type of FA used (C9-C12).
In (Fig. 4(d)), we report the oligomer ratios (of the same size) between the control and FA n set-ups. We considered four oligomers for this test: A 1, A 4, A 8, A 12; in each case, these ratios rise above the value of 1, showing that the presence of more below-CMC oligomers than on-pathway oligomers in the system as time increases. Note that these were generated from two stand-alone simulations, one for the control and the other one for FA n as in both cases only on-pathway oligomers are produced. The results clearly suggest that the FA n scenario produces more oligomers (and possibly more fibrils) with time which supports the experimental observation of increased ThT intensity for FA n . Additionally, as expected the ratios are smaller for larger oligomers; this is simply because of the reaction order as fewer oligomers of larger size are formed in the system. Thus our reaction hypothesis of a slowdown of the elongation stage in the presence of FA n s, although counter-intuitive, accurately validates the experimental observations of increased ThT intensity.
Dynamics in pseudo-micellar phase - Aβ/FA pm interactions
FA concentration around the CMC increases the concentration of FA pm in the bulk solution, the presence of which shifts the reaction flux towards predominantly off-pathway as established experimentally22, 51 (Fig. 1). As the forward elongation rate constant becomes crucial only at the later phase of the on-pathway reactions, the effect of K was neglected here. This allowed us to assume that effectively all FAs convert to FA pm s and thus making the effects of free FAs during the on-pathway elongation stage negligible. The predicted rate constants from the control experimental data were used here for the on-pathway set of reactions, and the parameter space was iterated to only estimate the rate constants involving off-pathway reactions. Thus, our combined on- and off-pathway model was able to explain all the experimental observations in the presence of FA pm (corresponding R 2 values are reported in Table 2 for C9-C12 FAs). Furthermore, previous reports identified that 12–24 mers (LFAOs) are the predominant species generated in the presence of C12 FA pm 22, 51. Our simulations predict the presence of multiple conformers within the oligomer of a specific size (strains) as observed before52. The 12–24 mer LFAOs show conformational dynamics (represented as and here), wherein the , and not , is able to associate with itself. Note that both species ( and ) correspond to 12–24 mer LFAOs and we considered up to four-fold lateral associations between them to form which are essentially 48–96 mers as previously established52. Such LFAOs then undergo a secondary fragmentation to produce four which are not allowed to laterally associate thereby making the 12–24 mers as the predominant species. This is due to subtle yet distinct structural differences between the two 12–24 mer LFAOs53. In our model, we identified that lateral association and fragmentation are both critically important in Aβ/FA pm reaction dynamics, which ultimately explains the formation of stable oligomers of size 12–24 i.e., . The predicted concentration of pseudo-micelles are shown in Table 2 while the predicted rate constants and shown in Supplementary Table 4 in Appendix-D under Supplementary Information.
Overall simulation of the ThT intensity data for FA pm regime yielded good correspondence with the experimental results for C12 (Fig. 5(a); green). We obtained a high R 2 value and nearly similar correspondence with the experimental lag time and saturation time for C12 as well for C9-C11 FAs (Fig. 5(b)). The comparative concentration dynamics of off-pathway and on-pathway aggregates in the presence of FA pm are shown in Fig. 5(c–f) for C12 fatty acid. Figure 5(e) is the data for constant FA pm and varying Aβ monomer entry rates while Fig. 5(f) is the data obtained for constant monomers and varying FA pm entry rates. In our model, for the sake of simplicity and comparison, we considered aggregates of similar sizes from both on- and off-pathway. We observe that the on-pathway oligomers A 4, A 7 and A 12 show delayed emergence (Fig. 5(c)). It is noteworthy that A 4 emerges rapidly, and saturates before decreasing in concentration. This behavior is due to the elongation of oligomers by A 12s that occurs along the on-pathway, which form larger fibrils at the cost of A 4 consumption. Along the off-pathway, aggregates , and show expected characteristics of being formed rapidly initially before saturating (Fig. 5(d)). It is important to note that the elongation in the off-pathway leads to consuming and oligomers to form the 12–24 mers as discussed previously. Such then associate to form which are seen in greater quantities towards the beginning with their concentration decreasing with time, which is likely due to fragmentation of high molecular weight aggregates. Finally, most of the FA pm dynamics leads to the formation of the with time, which becomes the predominant ThT positive species in the system. This dynamics is further corroborated by incorporation of physiologically relevant monomer entry rates into the system (Fig. 5(d)). Here, the dynamics show more conclusively the dominance of the oligomer in the system with increasing time followed by and . In order to verify the potential fragmentation identified by our simulation, we investigated this possibility experimentally by incubating Aβ and C12 FA near its CMC (Fig. 6). The results indicate that incubation rapidly results in the formation of aggregates corresponding to 50–60 nm diameter within one hour of incubation (Fig. 6(a) and (b)). However, after two hours of incubation, the size of the aggregates decreased to 10 nm diameter. No more change in the aggregate size was observed even after 48 h (data not shown). This phenomenon was also observed in the aggregate size monitored by immunoblotting (Fig. 6(c)), and supports the possibility of fragmentation of aggregates as predicted by the EKS model predictions.
Figure 5(e) and (f) show the ratio of the oligomer distributions for C12 fatty acids considering constant entry rates of Aβ monomers with fixed initial FA pm concentration (Fig. 5(e)) and FA pm into the system for fixed initial monomer concentration (Fig. 5(f)), respectively, as described before for FA n . These plots illustrate the switching behavior between the on- and off-pathways as predicted by the ROM stability analysis (Figs 2 and 3). For example, we can observe that for negligible monomer or FA pm entry rates, the on-pathway is active, but the switching occurs quickly to off-pathway when the monomer entry rate is about 0.001 × 100 μM h −1 (Fig. 5(e)). This switching effect is however faster when the FA pm entry rate is considered as shown in Fig. 5(f) (the amplitude of the ratio is higher in the latter case). Also, the ratios are higher after 50 h of incubation as compared to after 100 or 200 h (Fig. 5(e) and (f)). This suggests that either the concentration decreases with time or the A 12 increases with time or both cases happen simultaneously. As shown in Fig. 5(d), the increase in A 12 is more pronounced that that in concentration with time (note that both concentration increase with time) resulting in a decrease in the recorded ratio with time. Nevertheless, we can conclude that generally the switch to off-pathway is extremely fast with higher FA pm entry rates being a more crucial factor than an increase in the monomer entry rates for the observed effect.
Comparing the FA pm ThT intensity plots (Fig. 5(b)), we additionally observe that the concentration of FA pm significantly affects the behavior of aggregation. For example, higher the FA pm concentration present in the system (correlates with smaller fatty acid chain length), more the saturation is delayed for the ThT growth curve. As expected, our predicted FA pm concentration is highest near the CMC, and the concentration starts to decrease when total FA concentration is either increased or decreased. The predicted FA pm concentration for each fatty acid chain length at the near CMC zone experiments is shown in Table 2. Note that the prediction for the C9 case shows a small deviation primarily because the experiment was done at a 100 mM concentration while the actual CMC was 85 mM, i.e., a deviation of 17.6%.
Dynamics in micellar phase - Aβ − FA m interactions
The above-CMC regime (FA m ) only produces along the off-pathway that do not seem to aggregate any further due to the stabilization by the micelles22 (Fig. 7(a)). Therefore, the corresponding reaction model only considers the pre-nucleation stage in the off-pathway. Furthermore, the oligomers do not bind to ThT and hence, are invisible to this assay. This complicates the precise predictions of concentrations and direct correlation with the experimental data. Therefore, we could not estimate the accurate rate constants for FA m . It is noteworthy that we assumed the off-pathway reactions in the presence of FA pm also undergoes similar pre-nucleation stage reactions besides others. The rate constants of the pre-nucleation stage from FA pm data was used for this simulation to determine the dynamics of production. As shown in Fig. 7(b) and (c), one could observe that is generated rapidly accompanying a switching to the off-pathway, even with negligible monomer or micelle entry rates. As the formation presumably involves a concerted single step reaction, the concentration profile is different from its on-pathway counterpart, A 4. Specifically, as micelle entry rate increases, number of micelles in the system also increases such that the concentration increases much faster than that of the A 4 concentration as shown in Fig. 7(c). However, Fig. 7(c) suggests that for a fixed initial micelle concentration, and as the Aβ monomer entry increases, the ratio of goes towards saturation. This is potentially due to reduction in the number of micelles available for accommodating Aβ on the micellar surface.
The results reported here by two independent methods (ROM and EKS) reaffirm the experimental observations that FAs modulate Aβ aggregation. Specifically, the following significant conclusions can be drawn from this work: (a) interactions of Aβ with non-micellar FAs (FA n ) generate predominantly high molecular weight, on-pathway aggregates. (b) Interactions of Aβ with FAs near the latter’s CMC (FA pm ) generate predominantly low molecular weight oligomers (12–24 mers) along off-pathway along with some minor quantities of on-pathway fibrils. (c) Interactions of Aβ with high concentrations of FAs (FA m ; above their CMCs) exclusively generate off-pathway 4–5 mer oligomers without any fibrils suggesting that the on-pathway was completely turned off.
Perhaps most significantly, simulations from both ROM and EKS, as well as the previously demonstrated experimental evidence have identified that both concentration and phase-transitions of FAs dictate switching of Aβ aggregation pathways. Furthermore, the report demonstrates that under three distinct phases, FA n , FA pm , and FA m , the FAs not only modulate Aβ aggregation pathways but also promote low molecular weight oligomers, which are increasingly known to be the main pathogenic species in AD and other related neurodegenerative disorders. Specifically under pseudo-micellar conditions, where FA pm undergoes a distinct phase transition, they preferentially promote and stabilize low-molecular weight oligomers. The ability of certain phases of FAs, or in general any biological surfactants, to promote the formation of low molecular weight, off-pathway presents high significance in AD pathology. This is because oligomers generated in this manner could lead to the formation of various toxic ‘conformeric strains’ of Aβ aggregates, which could manifest in distinct phenotypic manifestations observed in AD. Indeed, Dean et al., have shown that LFAOs, which are generated in the presence of C12 FAs, are able to propagate morphologically distinct fibrils and caused excessive cerebral amyloid angiopathy (CAA) in transgenic mice brains, which was absent in similar reactions seeded with on-pathway fibrils52.
Mechanistically, simulations reported here have revealed a deeper understanding of Aβ-FA interactions. Some of the salient dynamics involved in the modulation of Aβ aggregation by FAs in distinct phases include: (i) in the presence of FA n , the free FAs seem to slow the kinetics of elongation (or post-nucleation) stage along the on-pathway, (ii) in the presence of FA m , aggregation does not proceed beyond (or ) likely due to the stabilization of Aβ by FA m as Aβ-FA mm complex. Further investigation of this complex by molecular dynamics based studies to infer why such complexes cannot aggregate is on-going and will be reported at a later date, and (iii) in the presence of FA pm , Aβ forms A 4 during the pre-nucleation phase followed by a slower progression to , and elongation to form 12–24 mers denoted by . Association of to form , which is thermodynamically unstable, leads to a fragmentation to form as supported by experimental evidence (Fig. 6). The most likely possibility is that the are structurally distinct and different from that are trapped in some kinetic minimum along the off-pathway as thought to be22, 51, which renders them incapable of aggregating further.
In addition to these insights, EKS model also considered a physiological scenario in which instead of constant amount or Aβ or FAs, a constant influx of Aβ monomers as well as pseudo-micellar and micellar FAs were considered. Incorporating specific entry rates for Aβ and FAs, we observe that the presence of pseudo-micellar phase influences the switching of pathways more than that induced by the monomers. However, we observed that even a slight increase in the entry rates of either FA or Aβ is enough to switch the dynamics preferentially towards the off-pathway. This is evident in Fig. 8, which shows a surface plot which considers the monomer and FA pm /FA m rates. The corresponding oligomer concentration ratios along off- and on-pathway show that the on-pathway is active only when FA pm entry rates are very low (Fig. 8(a) and (b)). After 50 h of incubation, off-pathway is predominantly activated for the highest FA pm entry rate and low monomer entry rates. In other words, all monomers entering the system are consumed by FA pm leaving little scope for on-pathway reaction to occur. However, after 200 h, off-pathway is most active when both FA pm and monomer entry rates are high resulting in most monomers being consumed by the FA pm . The scenario is bit different for FA m , where the off-pathway is most active when both monomer and FA m entry rates are high both after 50 and 200 h (Fig. 8(c) and (d)). The dynamics seems to be more stable after 200 h than after 50 h, as oligomer concentrations saturate by then. However after 50 h, a switch to off-pathway happens only with sufficiently high Aβ monomer and FA m entry rates, and there may be a critical point in this entry rate for both that would dictate whether a switch to the off-pathway occurs or not. In later times though (200 h, for example), there is a higher propensity of the system to switch to the off-pathway even with lower monomer or micelle entry rates.
Overall, this report has brought forth several interesting observations on the dynamics of Aβ-FA interactions, which seems to depend critically on the concentration and phase of FAs. Furthermore, the switching dynamics in conjunction with the constant infusion of Aβ monomers and FAs in their distinct phases illustrate the potential of biological interfaces to influence Aβ into forming several different conformationally distinct aggregates. Such conformeric strains could then influence phenotypic outcomes in AD pathology.
Preparation of large fatty acid-derived oligomers (LFAOs)
C12:0 fatty acid (5 mM) with 50 mM NaCl was added to freshly purified Aβ monomer (50 μM) buffered in 20 mM Tris, pH 8.0, and kept at 37°C in quiescent conditions. Separate reactions were started in a staggered fashion such that at the time of data collection, the sample would reach the desired time point.
Dynamic light scattering (DLS)
DLS data was collected using a Zetasizer Nano S instrument (Malvern, Inc., Worcestershire, UK). At each time point, DLS was collected by averaging 24 runs of 5 s each with a pre equilibration time of 30 s. This average was used to determine the diameter (nm) using the volume (%) function. For determining micelle size without Aβ, stock concentrations of C9:0, C10:0, C11:0, and C12:0 were diluted to the appropriate concentration in 50 mM NaCl buffered in 20 mM Tris, pH 8.0.
SDS-PAGE with immunoblotting
LFAO preparations were staggered in start such that at the time of initiating SDS-PAGE experiments, each sample was at the desired time point. Samples were diluted into 1X Laemmli loading buffer with 1% SDS, and loaded onto 4–20% BioRad TGX gels without boiling. Pre-stained MW markers (Novex Sharp Protein Standard, Life Technologies) were run in parallel for MW determination. Proteins were transferred to 0.2 μm nitrocellulose membrane (BioRad) and boiled for 1 min in a microwave oven in 1X PBS, followed by blocking for 1.5 h in 1X PBS containing 5% nonfat dry milk with 1% tween 20. Blots were then probed overnight at 4 °C with a 1:6000 dilution of Ab5 monoclonal antibody, which detects amino acids 1–16 of Aβ. Blots were then incubated with a 1:6000 dilution of anti-mouse, horseradish peroxidase conjugated secondary antibody and developed with ECL reagent (Thermo Scientific).
Reduced Order model (ROM)
The system of differential equations corresponding to Eqns 1 determines the evolution of the 4 species () and are given by
The above equations are better suited for analysis in non-dimensional form. If we chose the characteristic concentration and time as A 0 (the equilibrium concentration of monomers) and , respectively, then the dimensionless variables can be defined as follows:
The corresponding dimensionless differential equations, in terms of dimensionless time ‘s’, now can be written as
However, the on-pathway cannot be switched off and always persists. In this case the equilibrium solution is given by the pair .
Turning our attention now to the more general case of the non-dimensional equations (9–12), the equilibrium points, can be obtained by the vanishing of the equations (9–12). Solving for these four simultaneous equations, we obtain the following relations, in terms of the equilibrium concentration B 1,e
The subscript e indicates that these species are in equilibrium.
It should also be noted that if one treats the on- and off-pathway dynamics as a “competition” in accordance with the term used in game-theory54, 55, then the equilibrium is nothing but the Nash equilibrium of the system given by
(see Fig. 3 for an example of such an equilibrium). This equilibrium exists only at α 3 = 1 which also will be seen to have special significance in terms of the stability of the system. It follows that when α 3 > 1, then the off-pathway species dominate creating more equilibrium concentrations of and for α 3 < 1, on-pathway dominates resulting in greater production of .
We define and so that the former leads to off-pathway and the latter to on-pathway. Table 3 below points to a sample case of the various strategies in the competition between the two pathways with the pay-off for each “strategy” given by the equilibrium concentrations.
Time evolution of the different species
Linear stability analysis
To study the stability of the four species, we linearly perturb the system which is mathematically represented by
then the linearized set of differential equations for the perturbations, at o(ε) becomes
which can be expressed in operator form with the perturbation matrix, B given by
The stability of the equilibrium is found from computing the eigenvalues, λ i (i = 1, 2, 3, 4) of the matrix B. This is best done numerically. In the figures below we discuss the results of the computations. In particular, the term α 3 is varied throughout the analysis since it captures a significant dynamical feature of this problem; the switching from on-pathway to off-pathway. Over all, we choose the following ranges for our parameters: for i = 1, 2. Also, and . We begin with the base case defined by the choice , , n = 4 and B 1,e = 1 and then study the sensitivity of the system and our results to each of the parameters.
Ensemble Kinetic Simulations (EKS) of on- and off-pathways
Experimental observations and model assumptions
The detailed simulations and subsequent analysis are based on prior work where the in-vitro experiment using the non-esterified fatty acids of chain length C9 to C12 revealed a few crucial characteristics of Aβ42 aggregation22:
At FA concentration less than CMC range (FA n ), only on-pathway aggregates were produced. Furthermore an increase in ThT intensity than the control experiment was detected.
At near CMC (FA pm ), off-pathway fibrils of length 12–24 mers were noticed with very few fibrils being produced leading to the conclusion that the on-pathway was almost switched off.
At FA concentration higher than CMC (FA m ), off-pathway oligomers of length 4–5 mers were observed while no fibrils were produced, i.e., the on-pathway was switched off completely.
Before establishing a proper modeling of the off-pathway, we wish to explore the formation of micelles first. Ideally, CMC is the concentration of the surfactants (here, FAs) in the solution from where the micelle formation starts. Therefore, if high concentration of surfactants are introduced into the solution (FA m ), it directly forms micelles. At FA n and FA pm concentrations, ideally there should not be any micelles present in the solution whereas at FA m range, almost all of the FAs should convert into micelles. As discussed earlier, at FA pm , the FAs loosely form pseudo-micelles that are six-seven times larger than regular micelles. It can hence be hypothesized that as the FA concentration is increased beyond the CMC, extra FA molecules bind to the pseudo-micelles, which may then undergo structural change to form more stable and compact structured micelles (i.e., L) that were considered in the ROM model.
Based on the experimental observations, we make the following considerations to formulate the underlying reaction mechanism.
At FA n range, only on-pathway reactions take place, as in this zone no micelles are present in the solution. We assume that the presence of FAs have catalytic effects in altering the rate constants of the on-pathway with a factor of K resulting in a change in the final fibril concentration; the corresponding set of reactions are shown in Appendix-C under Supplementary Information.
At FA m concentrations, both on-pathway and off-pathway occur simultaneously; however, as seen in the ROM model, the existence of micelles can switch the Aβ monomers more towards the off-pathway aggregates. We further assume that one micelle binds four Aβ monomers at once to form the off-pathway species ; such oligomers cannot aggregate any further. This is because our experimental observations at FA m range point to the formation of which do not aggregate to form fibrils.
Similarly at FA pm range, both on- and off-pathway reactions occur simultaneously. The reaction mechanism for oligomer formation is similar as in the previous step. However, in this case the oligomer further aggregates to form 12–24 mers.
On-pathway reaction model
The on-pathway reaction model is motivated by our previous works in refs 9, 10, 47, 52, 56. Here, two different sets of reactions are considered. Firstly, the Aβ monomers form higher order aggregates (A 12) through monomer addition and eventually form fibrils; the modeling abstraction considers A 12 as fibrils, F. These reactions mainly depend on the monomer concentration and are slow being termed as pre-nucleation. Next, the higher order oligomers (A 12) react with the monomers and other on-pathway aggregates (A 2, …, A 11) and get elongated. The rates of these reactions depend on both monomer and fibril concentration. The pre-nucleation stage is slower, whereas the elongation phase is rapid, thereby causing the sigmoidal growth of the fibril concentration over time. The nucleation number of on-pathway reactions is taken as 12 as reported in ref. 48; these reactions are shown in Appendix-B under Supplementary Information.
FA n reaction model
At the FA n range, we assume the reactions to be exactly similar to the on-pathway model; however the rate constants for each phase of the sigmoidal ThT growth curve were varied by a factor of K. The corresponding reaction model is shown in Appendix-C under Supplementary Information.
FA m reaction model
The proposed off-pathway reaction set is quite different from the on-pathway model and motivated by the experimental observations. In this model, at FA m range, the four Aβ monomers directly convert into the off-pathway oligomer () by reacting with micelles, L. These can not able elongated any further. These reactions are shown in Appendix-E under Supplementary Information.
FA pm reaction model
The modeling of off-pathway reactions at FA pm zone is more challenging however; there are no existing models for off-pathway reactions at this zone. Furthermore, the behavior of aggregation, although sigmoidal, is much dissimilar than the on-pathway reaction in terms of the faster time-scale involved; the formation of 12–24 mers in this zone exhibit considerably less lag time and saturation time. We consider the following reactions at this range. In the first stage denoted as primary nucleation, the form higher order oligomers () by monomer addition (i.e., adding A 1). In the next stage, further elongates with the intermediate oligomers (–) to form higher order oligomers (); this stage is termed as the elongation stage. Note that a is a modeling abstraction and is of variable length; it’s length ranges from 16 mers to 23 mers (considering the addition of each to A 12). This results in a 6-fold increase in the oligomer size hosted by the pseudo-micelles as compared to the micelles (which can only host upto , whereas pseudo-micelles can host 23 mers) and is consistent with the experimental observations on the diameters of pseudo-micelles and micelles. We next consider such oligomers to laterally associate and create bigger oligomers (); we term this stage as lateral association. As experimentally validated in Fig. 6, there is approximately a four-fold increase in oligomer size towards the beginning of the FA pm dynamics after which the oligomer size goes down to that of and ; hence it is highly likely that the laterally associated undergoes a secondary fragmentation into the lower order oligomer .
It is noteworthy that is a structurally different species than ; the latter can laterally associate while the former cannot. This assumption was necessary to fit with the experimentally observed ThT kinetics data. Considering the secondary fragmentation to produce instead of could not correlate the simulation plots with the experimentally observed ThT dynamics. The set of near-CMC reactions are shown in Appendix-D under Supplementary Information.
Thus, the model at the near-CMC range deals with several rate constants and parameters. There are four rate constants for on-pathway reactions: (i) forward and backward rate constants of pre-nucleation reaction (k nuon , k nuon_) from the on-pathway; (ii) forward and backward rate constants of elongation reactions (k fbon , k fbon_) from the on-pathway; (iii) forward and backward rate constants of pre-nucleation in the off-pathway (k con and k con_); (iv) forward and backward rate constants of nucleation reactions (k nouff , k nouff_); (v) forward and backward rate constants of elongation reactions (k fboff , k fboff_ ) (vi) forward and backward rate constants of lateral association reactions (k eloff , k eloff_ ); (vii) forward and backward rate constants of secondary fragmentation (k fagoff , k fagoff_ ); (viii) the fatty acid effect on the on-pathway rate constant parameter, K; and finally (ix) the pseudo-micelle concentration: p. Note that, the estimation of the pseudo-micelle concentration from the CMC values of fatty acids poses a different problem and needs controlled experimentation; however, it is biophysically not possible to exactly measure the pseudo-micelle concentration. We could only measure the diameter of pseudo-micelles. Hence we considered p as a free parameter along with the other rate constants to estimate the pseudo-micelle concentration.
It is difficult to estimate the proper rate constant values of all these parameters at once. Hence, we follow our divide and conquer strategy from ref. 10 to determine all the rate constants step by step. First, we matched the experimental data of the control experiment with the simulation considering the on-pathway reaction setup only and estimated the four rate constants involved in the on-pathway reactions. Subsequently, we used these values in the combined on-off-pathway model to estimate all the additional rate constants. More precisely, our simulation involves the steps below:
First, determine the rate constants for the on-pathway reactions from control experiment data.
Estimate the parameter K for the FA n experimental data by using the estimated on-pathway rate constants.
Estimate the rate constants of off-pathway aggregation with FA pm experimental data utilizing the estimated on-pathway rate constants above.
Validate the formation of off-pathway oligomers () in FA m concentration using all the estimated rate constants.
We first calculated the reaction flux for all reactions at a particular stage; then using these reaction fluxes, the differential equations for the rate of change of concentration is formulated for each oligomer. Next, these differential equations were solved using MATLAB’s ode solver and the R 2 value between the simulated curve and experimental data was calculated for the different rate constant combinations. We solved these differential equations for various rate constant combinations with each rate constant ranging from 10−5 to 105 and the pseudo-micelle concentration p from 1 to 100 μM with multiples of 5. After that, these rate constants were manually fine-tuned to better match the experimental data and obtain better rate constant estimates. The best-fitted simulation parameters are taken as estimated rate constants of these reactions; each of these parameters are reported in the Appendices under the corresponding reaction models. Moreover, all the rate constants estimated here by fitting with experimental ThT intensity plots required a mapping of the cumulative effects of the concentration of higher order ThT positive oligomers to the ThT values; such mapping is explained in Appendix-F in the Supplementary Information.
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The authors wish to thank the following agencies for financial support: National Center for Research Resources (5P20RR016476-11) and the National Institute of General Medical Sciences (8 P20 GM103476-11) from the National Institutes of Health for funding through INBRE (to VR), National Institute of Aging (R15AG046915) (to VR) and NSF Graduate Research Fellowship Program (NSF 1445151) and Gk-12 Program at USM (NSF 0947944) (to DND) and NSF 1351786 to PG.
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