Abstract
Milk production in dairy cow udders is a complex and dynamic physiological process that has resisted explanatory modelling thus far. The current standard model, Wood’s model, is empirical in nature, represents yield in daily terms, and was published in 1967. Here, we have developed a dynamic and integrated explanatory model that describes milk yield at the scale of the milking session. Our approach allowed us to formally represent and mathematically relate biological features of known relevance while accounting for stochasticity and conditional elements in the form of explicit hypotheses, which could then be tested and validated using real-life data. Using an explanatory mathematical and biological model to explore a physiological process and pinpoint potential problems (i.e., “problem finding”), it is possible to filter out unimportant variables that can be ignored, retaining only those essential to generating the most realistic model possible. Such modelling efforts are multidisciplinary by necessity. It is also helpful downstream because model results can be compared with observed data, via parameter estimation using maximum likelihood and statistical testing using model residuals. The process in its entirety yields a coherent, robust, and thus repeatable, model.
Similar content being viewed by others
Introduction
In milk-yield studies, Wood’s model is the standard of reference1,2,3, in part because of its good fit to observed data, but mostly because no better alternatives exist4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22. A study in 194723 first underscored the need for an explanatory model of milk production physiology to better understand and thus control udder mastitis24, 25, a problem that remains a major concern in the dairy industry; this need has yet to be met.
Historically, researchers have characterised milk production in cow udders by individually examining different system components, namely the dynamics of the alveolar population over the course of lactation26,27,28,29, of alveolar milk synthesis between milking sessions (accounting for different udder compartments and quarters)30,31,32,33,34, and of udder emptying during milking35,36,37. This work helped clarify the biological mechanisms at play. Subsequent research was able to go further in describing system components and demonstrating how they interacted in dairy cows. For example, scientists focused on the dynamics of alveolar populations and of milk synthesis38,39,40,41,42; the links between milk synthesis, udder compartments, and milking interval duration43,44,45,46,47,48,49,50,51,52; and the consequences of udder-emptying problems for milk yield53,54,55,56,57,58,59,60,61,62,63,64,65,66.
It is important to note that these biology-oriented models were based on daily milk yield rather than on session-specific milk yield. In 1991, Beever67 compared results obtained from empirical models with those from more mechanistic models, inspiring further research. Notably, Dijkstra26 (in 1997), Pollott29, 68 (in 2000 and 2004), and Hanigan69 (in 2007) developed daily-yield biological models in which parameter estimates could be adjusted to real-life observations. Other related work led to the creation and exploration of biological simulations of daily yield (e.g., Shorten70 in 2002). However, these models were overparameterised and thus parameter estimation from observed data was not possible.
Because the research described above was focused on daily milk yield and did not address udder-emptying dynamics or the precision of session-level measurements, it cannot be used to fully parse the variance associated with milk yield. Furthermore, such approaches cannot directly exploit data from automated milking systems, which are becoming more and more common. To obtain daily yields (i.e., for a 24-hour period) from such systems, additional calculations71, 72 must be used, which increases response variability.
Our goal was to exploit this previous research to construct a more holistic model of milk production. We therefore selected the information that was needed to properly represent per-session milk yield for a given animal over its entire lactation period. We used a maximum likelihood estimation approach and explicitly modelled the mean, variance, and covariance of the random variables that we studied. First, we present a simplified version of the model’s formalisation. We then provide and discuss the results. In the latter part of the methods, we address more technical details—the mathematics and statistics that were used to systematically develop the model.
Model formalisation
Here, we present a mathematical representation of this biological process that has deterministic properties; it also has stochastic properties, which are framed by probabilities, management of randomness, and the potential influence of unobserved conditional elements (see Fig. 1 for model structure and Methods for the detailed model description). In our biological study system, cows are milked for the first time shortly after calving. Then, cows are milked twice a day, in the morning and the evening (mean interval durations: 14 hours between evening and morning milkings and 10 hours between morning and evening milkings; see Supplementary Fig. 1). For the total lactation period and each milking session, we know milk yield (a quantity whose precision is established a priori) for the four quarters of the udder; we also know the date and time of each milking session.
In our model, the udder comprises a single quarter with a single compartment. Over a given lactation period, the size of the alveolar population is constant; it is only renewed between lactation periods. Alveoli belong to one of three classes, whose proportions change over the lactation period26, 29. Time is expressed in hours since the beginning of lactation, which occurs prior to calving. Since the precise timing of this moment is unknown, it is necessary to estimate δ, which is the number of hours between the start of lactation and the first milking session. Once milk production has begun, changes in alveolar class proportions are described using a system of ordinary differential equations (SODE) with two parameters to estimate: alveolar activation rate, λ A , and alveolar inactivation rate, λ R , which are constant over the lactation period. Milk yield is modelled by considering that milk production between two successive milking sessions is a function of interval duration, the proportion of activated alveoli during the interval, and milk secretion rate30, 32. The latter remains constant between successive milking sessions. When modelling milk extraction during the milking session, it is assumed that the udder is not fully emptied35,36,37; the degree of extraction depends on the milk retention rate, π. Because the retention rate and the secretion rate are constant over the lactation period, we can use the normalized estimated residuals to calculate the following: 1) variation in the retention rate for a specific milking session π(k), which corresponds to the physiological fact that extreme retention rates are seen during certain milking sessions; and 2) variation among intervals in the milk secretion rate PL(k), which corresponds to the physiological fact that secretion rate changes over the lactation period. We could thus determine the interval-specific proportions β(k) = PL(k)/max(PL(k)), which are constant within intervals. From a methodological standpoint, these two variation types are conditional elements linked to variation-generating factors that act over the lactation period and that are not necessarily characterized a priori. In the variance model, the total variance for each milking session is broken down into individual components of stochasticity. Therefore, we could obtain a component’s proportional contribution for each milking session and for the entire lactation period. We also estimated the variance associated with interval duration, which can be treated as a random variable for which only mean and variance are known. A model describing daily production (hereafter, the daily-yield model) was derived from the results of the finer-scale, milking-session model. Once validated, its findings were compared with those obtained using Wood’s model.
Results
The maximum likelihood estimation results (Tables 1 and 2 for milking-session model, and Table 3 for Wood’s model) are illustrated with data from the second lactation of a healthy Montbéliarde cow (i.e., never displayed symptoms of clinical mastitis).
Following model validation (Fig. 2), we obtained normally distributed standardized residuals for the milking-session model and the daily-yield model (Kolmogorov-Smirnov [KS] test: P-value = 0.4458 and P-value = 0.7131, respectively). The residuals were also uncorrelated (Box-Pierce test: P-value = 0.1599 and P-value = 0.1169, respectively). For Wood’s model, the residuals were normally distributed (KS test: P-value = 0.5817) but auto-correlated (P-value < 10−6); consequently, the confidence intervals and the statistical tests using estimated variances were unreliable.
The milking-session and daily-yield models appear to fit the observed data nicely (r = 0.93 and r = 0.92, respectively). For Wood’s model, the correlation was slightly weaker (r = 0.87). We then plotted the observed values alongside the estimated values obtained from three milking-session model types (i.e., morning milking, evening milking, and daily yield; ±95% CI). We estimated daily yield by aggregating the values for a day’s successive milking sessions; we then compared the results with those obtained using Wood’s model (Fig. 3).
Using the milking-session model, we estimated changes in the mean proportions of the alveolar classes over the lactation period. We also estimated changes in β (Table 4) and π (Table 5) over successive milking sessions. The estimated proportion of total variance explained by the milking-session model and the daily-yield model was 1.03 and 1.07, respectively. The variance model did a good job of accounting for total observed variance. Upon breaking down total variance, we found that variance in the proportion of activated alveoli made the largest contribution (total variance for the lactation period: 78.4%; total variance per session: Fig. 4).
When precise interval duration was unknown, the proportion of total variance explained by the milking-session model and the daily-yield model was 0.98 and 1.07, respectively; total variance was higher than previously. As before, the proportion of activated alveoli made the largest contribution to total variance overall (62.1%) and nearly always to session-specific variance. However, the variance due to the limited information on interval duration was the second largest contributor (18.8%; see Supplementary Fig. 4). We estimated also daily yield by aggregating the values for a day’s successive milking sessions; we then compared the results with those obtained using Wood’s model (see Supplementary Fig. 5). When precise interval duration was unknown, the daily-yield model, like Wood’s model, was subject to “smoothing”. In such cases, our model could obtain estimates of characteristic lactation traits, such as the length of peak lactation (28 days for our model vs. 47 days for Wood’s model) and peak milk yield (33.8 kg for our model vs. 33.2 kg for Wood’s model)73,74,75. Total milk production per lactation period was 6,749 kg in the observed data, 6,744 kg in our model, and 6,750 kg in Wood’s model.
A simulation was used to examine the influence of the model’s four main parameters—λ A , λ R , PL max , and π—on the mean and variance models, as well as on the main model validation criteria (see Supplementary Fig. 6).
Discussion
In summary, our biological model incorporates different sources of variability and provides a good fit for observed milking-session and daily yields. It uses data that are easy for livestock farmers to obtain, notably milk yields for all four quarters of the udder for each milking session and the length of time between successive sessions.
The proportion of activated alveoli over time is the largest contributor to variance; this variance component includes a dispersion parameter that accounts for underdispersion because of the interdependence of the alveolar classes. To characterise quarter-specific variability, quarter-specific milk yield per milking session must be quantified. Part of this variability could also stem from inconsistency in alveolar activation and inactivation rates across the lactation period or from the rates being quarter specific. However, our assumption of rate consistency was validated ex post because the model’s predictions agreed with the observed data.
Since interval duration is the main factor explaining milk yield, it needs to be described more precisely. When only mean duration is available, variance associated with this variable becomes the second greatest contributor to variance in milk yield. Consequently, it is clear that daily-yield models (where interval duration is a constant 24 hours) will provide less precise yield estimates. Moreover, such models cannot account for milk retention, meaning that the autocorrelation among residuals will render any statistical results unreliable; this is the case for yields obtained with Wood’s model. Our model could be used with automated milking systems (i.e., robotic systems) in which milking occurs every 8 hours on average, making it possible to model quarter-specific yields.
Our model is based on a small number of parameters that all have clear biological interpretations. It also accounts for the action of conditional elements via the use of two types of variation. Retention rate could explain the persistence of pathogenic agents in the udder and their subsequent migration into the alveolar compartment when the alveolus relaxes at the end of the milking session. Variation in β, which accounts for the influence of several potential conditional elements during the lactation period, can be used to test their effects a posteriori. It should be noted that interpreting β may be tricky because it is an umbrella variable that encompasses several different mechanisms, including disease-related udder problems. Furthermore, this model can be used to study inter- and intra-individual variability in parameter values (e.g., by incorporating information on a single animal’s different lactations). Ultimately, this model could also be extended to other dairy livestock species.
This approach includes a variance model that helps ensure the validity of the overall model over the course of its development. By using maximum likelihood estimation, we obtained estimates that do not violate statistical assumptions. This work underscores the advantages of using a multidisciplinary approach to tackle a biological question, where a model is fit to observed data and a minimal number of biological mechanisms are represented. By using a biological model as a source for generating mathematical models, statistical models, and computer models, we can help ensure that biological reality is reflected. Therefore, the scientific knowledge and biological hypotheses included in the model ultimately inform the final interpretation of the results downstream. The model can thus identify gaps in knowledge that must be filled to better understand key biological mechanisms. In short, using this modelling approach, we were able to reduce a “rather complex” system to a “somewhat complicated” system that is coherent and understandable.
Methods
Modelling mean yield per milking session
We treated the udder as comprising a single quarter and a single compartment with an alveolar population of constant size for a given lactation period; the population is renewed only between lactations. Alveoli are epithelial cells that secrete and store milk. The population is split into three classes whose relative abundances changed over the lactation period. These classes are as follows: (1) non-activated alveoli, NCI(t); (2) activated alveoli (i.e., capable of producing milk), NCA(t); and (3) inactivated alveoli, NCR(t). Time is expressed in hours since t = 0, or the beginning of lactation, which generally precedes calving. Since the precise moment lactation begins is unknown, we estimated δ, or the length of time between the beginning of lactation and the first milking session. If alveolar population size, NCT, is constant over the lactation period, then, at any given moment, NCI(t) + NCA(t) + NCR(t) = NCT. We can therefore obtain the proportions of alveoli in the different classes at any point in time: NI(t) = NCI(t)/NCT; NA(t) = NCA(t)/NCT; and NR(t) = NCR(t)/NCT. When lactation begins, NI(0) = 1, NA(0) = 0, and NR(0) = 0. After lactation begins, and assuming alveolar activation and inactivation are random processes, changes in the relative proportions of the different alveolar classes can be described using a system of ordinary differential equations (SODE):
In this SODE, two parameters must be estimated: the rate of alveolar activation, λA, and the rate of alveolar inactivation, λR, which are considered to be constant over the lactation period.
The SODE has an explicit solution that is a function of the time since the beginning of lactation:
Milk secretion between successive milking sessions, denoted k and (k − 1), is a function of the time elapsed between the two, (t(k) − t(k − 1)); the proportion of activated alveoli during this interval, NA(t); the maximum volumetric capacity of the udder, Vmax; and the maximum secretion rate if all the alveoli are secreting milk, Pmax. The latter equals the product of per-alveolus secretion and the udder’s total number of alveoli, NCT, for a given lactation period. Milk secretion, YP(t), is therefore defined by the following differential equation:
If the compartment, the site of milk secretion, is considered to be empty at time t(k − 1) after milking session (k − 1), this equation has an explicit solution:
When milking occurs twice daily, as in our study system, where the mean time between successive milking sessions was 14 (evening to morning) and 10 hours (morning to evening), the udder is far from reaching maximum capacity. Consequently, Vmax and Pmax cannot be estimated. Furthermore, because Pmax is rather low, secretion can be approximated as follows:
In other words, when PL = Vmax · Pmax, PL can be estimated, and then milk secretion at any given moment is a linear function of the proportion of activated alveoli:
This equation has an explicit solution and yields the following after integration:
where
and
By definition, the parameter PL represents, to varying degrees, the udder’s total number of alveoli, udder maximum capacity, and the milk secretion rate of activated alveoli. Its value can therefore vary over the lactation period. More generally, certain factors could result in PL being constant within intervals but variable over the lactation period. Because we have no information a priori regarding these factors, the goal is to determine if they are present and account for them in the model as necessary. Using the residuals from an initial model in which PL was assumed to be constant over the lactation period, we could choose the intervals to be included in the final model.
If we assume that the udder (i.e., cisterns) has not been completely emptied at the end of each milking session, then it clearly becomes important to consider the milk retention rate, or π, which is assumed to be constant over the lactation period. For a given milking session, k, the milk extracted, YE(k), is thus the sum of the milk produced since the previous session, YP(k), to which is added the residual milk (carryover) from the previous session YR(k − 1) and from which is subtracted the residual milk from the current session, YR(k). Therefore,
If we consider that
then
Here, we are thus assuming that the probability of milk extraction during a given session is (1 − π). Using the residuals from an initial model in which this parameter was held constant over the lactation period, we could identify any extreme retention rate values during milking sessions. Including retention rate in the model allows us to account for the autocorrelation commonly observed in milk yield over successive sessions.
Using a model in which milk retention rate, π, and milk production rate, PL, are constant over the lactation period, we can exploit the normalized estimated residuals to characterize the following sources of variance:
-
(1)
Variance in the milk retention rate that is specific to a given milking session, which reflects the physiological reality that retention rates can be extreme for certain sessions. Extreme values were considered to occur when the standardized residual had a probability of less than 0.001 of occurring. These cases were estimated in the final model by considering rate values that were “too” different from the mean rate.
-
(2)
Variance over time in the secretion rate, which reflects the physiological fact that the values of parameters associated with milk secretion change over time. Such variance can be identified by analysing changes in the mean and variance of the model’s standardized residuals over the course of successive milking sessions. This task was accomplished using the cpt.meanvar function in the R package changepoint76, with which probable parameter change points can be statistically identified (alpha level of 0.05). We thus obtained the parameter PL(k), which is constant within intervals over the lactation period. Since PLmax = max(PL(k)), we could introduce the parameter β(k), which is equal to PL(k)/PLmax and varies between 0 and 1. It therefore characterises the udder’s milk production potential over the lactation period. However, it is somewhat more difficult to interpret β(k) because, by definition, it represents the effects of several different physiological mechanisms. For instance, its value is influenced by the secretion rate, which can vary over the lactation period based on an animal’s diet, by udder swelling when an animal is put out to graze, by the proportion of activated alveoli, and by milk “build-up” when the alveolar compartment contracts as milking begins.
By accounting for these two types of variation in the model, we obtained independent, standardized residuals that follow a normal distribution, making it possible to validate the adjusted model. From a methodological standpoint, the two categories of variation correspond to conditional elements, which are factors that introduce variation over the lactation period and for which precise information is rarely available beforehand. Modelling such variance may therefore be helpful in identifying such factors a priori and thus making it possible to test their effects a posteriori.
Using estimated milking-session means and variances (obtained exclusively when residuals are uncorrelated), we could estimate daily means and variances for milk yield, by summing the results for a day’s morning and evening sessions. We then validated this daily-yield model using estimated normalised residuals from the milking-session models.
Wood’s model, which is an empirical model that describes the daily lactation curve of a dairy cow, is the standard reference model. It employs a gamma function that mirrors milk yield over the course of a day x j :
However, its three parameters (a, b, and c) do not have a clear biological interpretation and the parameters are estimated using least squares.
Modelling variance in yield per milking session
To model session-specific variance in yield, we broke down total variance into its different components. For the variance associated with measurement error, we considered that, for each milking session k, the amount of milk measured, or YM(k), is a random variable that follows a log-normal distribution, whose level of precision is determined beforehand using a coefficient of variation, CVM, equal to 0.025 (or 2.5%). The model describing measured milk yield is as follows: Y M (k) = Y E (k) · exp(ε), where E(ε) = 0 and Var(ε) = CV M 2. The mean is calculated using E(Y M (k)) = E(Y E (k)), since when |ε| < 1, \(E(exp(\varepsilon ))=exp(E(\varepsilon ))=1.\) To find the variance, we use
where YE(k) is also a random variable.
For the variance associated with the amount of milk extracted, YE(k), we consider that, for each milking session k, YE(k) is a random variable whose mean and variance are estimated using
and
respectively.
The milk produced since the previous milking session, YP(k), is also a random variable. Calculating the variance in YE(k) requires an iterative approach involving the variance in the amount of residual milk:
where E(YR(0)) = 0 and Var(YR(0)) = 0.
Variance in milk secretion within intervals, YP(k), can be due to either random variability in β(k) or random variability in the mean proportion of activated alveoli, MNA(k), for the current interval. Therefore, the mean can be found as follows:
where
Variance is equal to
where
and
The latter equation includes a parameter, ϕ, that must be estimated. It is a dispersion parameter that can handle underdispersion and thus account for interdependence among alveoli.
For a given milking session, total variance Var(Y M (k)) can be broken down into variance due to measurement error,
variance due to milk retention,
milk carryover,
the proportion of PLmax,
and the proportion of activated alveoli,
Therefore, for each milking session, we obtained:
We quantify the relative contribution of each variance component by dividing the component’s value by total variance, for each milking session and for the full lactation period. To determine the amount of variance explained by the model, we find the ratio of the total variance as calculated above to the total residual variance remaining after maximum likelihood estimation.
We also modelled the variance associated with interval duration by treating the latter as a random variable for which only the mean and variance are known. The relevant equations are as follows:
where
Δt(k) = 14.0 if k is a morning session, Δt(k) = 10.0 if k is an evening session and Var T = 0.25.
For a given milking session k, total variance is therefore:
Applying and validating the model
Model parameter estimation was carried out using the maximum likelihood estimator obtained from the milking-session model. Consequently, if YO(k) is observed milk yield for milking session k, then the model’s standardised residuals, (Y O (k) − E(Y M (k)))/Var(Y M (k))1/2, follow a normal distribution if the model is accurate. The model parameters were therefore estimated using the maximum likelihood estimator, by integrating the mean and variance models. To ensure the different parameters had valid values, we used the appropriate transformations. For instance, when a parameter ϴ can only have positive values, we used the transformation ϴ = exp(α), where the domain of α is all real numbers. When a parameter ϴ is only meaningful at values between 0 and 1, we used the transformation ϴ = exp(−exp(α)), where the domain of α is once again all real numbers.
The nlm function in R was used to minimize the model’s maximum likelihood function (see Supplementary Note: “Parameter estimation using the nlm function in R.” online). We obtained the estimator variances along the main diagonal by inversing the Hessian matrix. The parameters’ variances and 95% confidence intervals were then calculated after inversely transforming their estimates and their bounds, by exploiting the normality of the maximum likelihood estimators.
We then validated the milking-session and daily-yield models using an analysis of the model’s standardized residuals, which were normally distributed and uncorrelated, meeting statistical assumptions. Normality was tested using a Kolmogorov-Smirnov (KS) test. A Box-Pierce77 test was used to confirm that the residuals were not auto-correlated. The results were visualised by plotting the empirical cumulative distribution function (R function ecdf) and estimates of the partial autocorrelation function (R function pacf), respectively.
We validated the models for session-specific means and variances by simulating different sets of milking-session observations while accounting for the different conditional elements included in the variance model. We obtained a large number of simulated datasets (n = 1,000), which allowed us to estimate session-specific means and total variances. Strong correlations between the estimates obtained from the models and the simulations for the means, total variances, and 95% confidence intervals helped validate the model structure used to arrive at the values of those statistics. Taken together, the simulated datasets obtained for the full lactation period allowed us to confirm the robustness of the milking-session and daily-yield models (see Supplementary Figs 2 and 3).
We determined the variance due to the parameter estimators by integrating the estimator variance-covariance matrix into the simulations. Over the lactation period, the variance associated with estimator precision represented 4% of the total estimated variance. We found that, for the first milking sessions, the greatest influence was wielded by the parameter estimating the duration δ between the beginning of lactation and the first milking session.
A pilot simulation program was developed to help visualise the influence of the milk-yield model’s four main parameters — λ A , λ R , PL max , and π — on the mean and variance models and on the principal criteria for model validation. It uses the R packages tcltk and tkrplot (see Supplementary Fig. 6).
Data Availability
The data are available in a ZIP file (4.28 MB), “ModelPL-SR-PG-20170425”, which is located in an INRA repository at the permanent web address: “http://epia.clermont.inra.fr/plsrpg”. In exchange for access to the file, we simply request that the interested party provide his or her email. The following information is included in the ZIP file. (1) The lactation data that was analysed in this manuscript and lactation data for two other dairy cattle species—the Holstein and the Montbéliarde. They are located in the “Data” directory; data on different lactation ranks (two to six) are also included. This information can be used to illustrate how the model can be generalised. (2) The numerical results and graphs in pdf format are located in the “GraphPDF” directory. (3) The R scripts we used are in the “ProgR” directory. They include the scripts for model parameter estimation, the simulation integrating random parameter variability, the automatic output of numerical results and graphs in pdf format, and the running of the pilot simulation. (4) In the latter directory, there is also a general script, “ScriptRgeneral.txt”, for implementing the other scripts in a logical order. We chose to leave all the scripts in R so that they are easy to read and evaluate if one has a basic understanding of the R language. All of the functions described above will ultimately be grouped into an R package once they have been optimized and once estimation procedures have been written in C. An example library for the Windows OS is included in “ProgC” directory.
References
Wood, P. D. P. Algebric model of the lactation curve in cattle. Nature 216, 164–165 (1967).
Wood, P. D. P. Factors affecting persistency of lactation in cattle. Nature 218, 894 (1968).
Wood, P. D. P. Factors affecting the shape of the lactation curve in cattle. Animal Production 11, 307–316 (1969).
Scherchand, L., McNew, R. W., Kellogg, D. W. & Johnson, Z. B. Selection of a mathematical model to generate lactation curves using daily milk yields of Holstein cows. Journal of Dairy Science 78, 2507–2513 (1995).
Scott, T. A., Yandell, B., Zepeda, L., Shaver, R. D. & Smith, T. R. Use of Lactation Curves for Analysis of Milk Production Data. Journal of Dairy Science 79, 1885–1894 (1996).
Olori, V. E., Brotherstone, S., Hill, W. G. & McGuirk, B. J. Fit of standard models of the lactation curve to weekly records of milk production of cows in a single herd. Livestock Production Science 58, 55–63 (1999).
Grossman, M., Hartz, S. M. & Koops, W. J. Persistency of lactation yield: A novel approach. Journal of Dairy Science 82, 2192–2197 (1999).
Vargas, B., Koops, W. J., Herrero, M. & Van Arendonk, J. A. M. Modeling extended lactations of dairy cows. Journal of Dairy Science 83, 1371–1380 (2000).
Landete-Castillejos, T. & Gallego, L. The ability of mathematical models to describe the shape of lactation curves. Journal of Animal Science 78, 3010–3013 (2000).
Rekaya, R., Carabaño, M. J. & Toro, M. A. Bayesian analysis of lactation curves of Holstein-Friesian cattle using a nonlinear model. Journal of Dairy Science 83, 2691–2701 (2000).
Jaffrezic, F., White, I. M. S., Thompson, R. & Visscher, P. M. Contrasting models for lactation curve analysis. Journal of Dairy Science 85, 968–975 (2002).
Grossman, M. & Koops, W. J. Modeling extended lactation curves of dairy cattle: A biological basis for the multiphasic approach. Journal of Dairy Science 86, 988–998 (2003).
Togashi, K. & Lin, C. Y. Modifying the lactation curve to improve lactation milk and persistency. Journal of Dairy Science 86, 1487–1493 (2003).
Druet, T., Jaffrézic, F., Boichard, D. & Ducrocq, V. Modeling lactation curves and estimation of genetic parameters for first lactation test-day records of French Holstein cows. Journal of Dairy Science 86, 2480–2490 (2003).
Macciotta, N. P. P., Vicario, D., Di Mauro, C. & Cappio-Borlino, A. A multivariate approach to modeling shapes of individual lactation curves in cattle. Journal of Dairy Science 87, 1092–1098 (2004).
Val-Arreola, D., Kebreab, E., Dijkstra, J. & France, J. Study of the lactation curve in dairy cattle on farms in central Mexico. Journal of Dairy Science 87, 3789–3799 (2004).
Macciotta, N. P. P., Vicario, D. & Cappio-Borlino, A. Detection of different shapes of lactation curve for milk yield in dairy cattle by empirical mathematical models. Journal of Dairy Science 88, 1178–1191 (2005).
Silvestre, A. M., Petim-Batista, F. & Colaço, J. The accuracy of seven mathematical functions in modeling dairy cattle lactation curves based on test-day records from varying sample schemes. Journal of Dairy Science 89, 1813–1821 (2006).
Dematawewa, C. M. B., Pearson, R. E. & VanRaden, P. M. Modeling extended lactations of Holsteins. Journal of Dairy Science 90, 3924–3936 (2007).
Adediran, S. A., Ratkowsky, D. A., Donaghy, D. J. & Malau-Aduli, A. E. O. Comparative evaluation of a new lactation curve model for pasture-based Holstein-Friesian dairy cows. Journal of Dairy Science 95, 5344–5356 (2012).
Murphy, M. D., O’Mahony, M. J., Shalloo, L., French, P. & Upton, J. Comparison of modeling techniques for milk-production forecasting. Journal of Dairy Science 97, 3352–3363 (2014).
López, S. et al. On the analysis of Canadian Holstein dairy cow lactation curves using standard growth functions. Journal of Dairy Science 98, 2701–2712 (2015).
Little, R. B. & Plastridge, W. N. In Bovine Mastitis. A symposium. Preface (McGraw-Hill Book Company, 1946).
Gasqui, P., Coulon, J.-B. & Pons, O. An individual modelling tool for within and between lactation consecutive cases of clinical mastitis in the dairy cow: an approach based on a survival model. Veterinary Research 34, 85–104 (2003).
Coulon, J.-B. et al. Effect of mastitis and related-germ on milk yield and composition during naturally-occurring udder infections in dairy cows. Animal Research 51, 383–393 (2002).
Dijkstra, J. et al. A model to describe growth patterns of the mammary gland during pregnancy and lactation. Journal of Dairy Science 80, 2340–2354 (1997).
Wilde, C. J., Quarrie, L. H., Tonner, E., Flint, D. J. & Peaker, M. Mammary apoptosis. Livestock Production Science 50, 29–37 (1997).
Knight, C. H., Peaker, M. & Wilde, C. J. Local control of mammary development and function. Reviews of Reproduction 3, 104–112 (1998).
Pollott, G. E. A Biological Approach to Lactation Curve Analysis for Milk Yield. Journal of Dairy Science 83, 2448–2458 (2000).
Knight, C. H., Hirst, D. & Dewhurst, R. J. Milk accumulation and distribution in the bovine udder during the interval between milkings. Journal of Dairy Research 61, 167–177 (1994).
Knight, C. H. & Dewhurst, R. J. Once daily milking of dairy cows: relationship between yield loss and cisternal milk storage. Journal of Dairy Research 61, 441–449 (1994).
Davis, S. R. et al. Partitioning of milk accumulation between cisternal and alveolar compartments of the bovine udder: relationship to production loss during once daily milking. Journal of Dairy Research 65, 1–8 (1998).
Davis, S. R., Farr, V. C. & Stelwagen, K. Regulation of yield loss and milk composition during once-daily milking: a review. Livestock Production Science 59, 77–94 (1999).
Stelwagen, K. Effect of milking frequency on mammary functioning and shape of the lactation curve. Journal of Dairy Science 84, E204–E211 (2001).
Bruckmaier, R. M. Milk ejection during machine milking in dairy cows. Livestock Production Science 70, 121–124 (2001).
Bruckmaier, R. M. & Hilger, M. Milk ejection in dairy cows at different degrees of udder filling. Journal of Dairy Research 68, 369–376 (2001).
Bruckmaier, R. M., Macuhova, J. & Meyer, H. H. D. Specific aspects of milk ejection in robotic milking: a review. Livestock Production Science 72, 169–176 (2001).
Capuco, A. V., Wood, D. L., Baldwin, R., Mcleod, K. & Paape, M. J. Mammary cell number, proliferation, and apoptosis during a bovine lactation: relation to milk production and effect of bST. Journal of Dairy Science 84, 2177–2187 (2001).
Nørgaard, J., Sørensen, A., Sørensen, M. T., Andersen, J. B. & Sejrsen, K. Mammary cell turnover and enzyme activity in dairy cows: Effects of milking frequency and diet energy density. Journal of Dairy Science 88, 975–982 (2005).
Sorensen, M. T., Nørgaard, J. V., Theil, P. K., Vestergaard, M. & Sejrsen, K. Cell turnover and activity in mammary tissue during lactation and the dry period in dairy cows. Journal of Dairy Science 89, 4632–4639 (2006).
Fitzgerald, A. C., Annen-Dawson, E. L., Baumgard, L. H. & Collier, R. J. Evaluation of continuous lactation and increased milking frequency on milk production and mammary cell turnover in primiparous Holstein cows. Journal of Dairy Science 90, 5483–5489 (2007).
Pollott, G. E., Wilson, K., Jerram, L., Fowkes, R. C. & Lawson, C. A noninvasive method for measuring mammary apoptosis and epithelial cell activation in dairy animals using microparticles extracted form milk. Journal of Dairy Science 97, 5017–5022 (2014).
Ayadi, M., Caja, G., Such, X., Rovai, M. & Albanell, E. Effect of different milking intervals on the composition of cisternal and alveolar milk in dairy cows. Journal of Dairy Research 71, 304–310 (2004).
Bach, A. & Busto, I. Effects on milk yield of milking interval regularity and teat cup attachment failures with robotic milking systems. Journal of Dairy Research 72, 101–106 (2005).
Hickson, R. E., Lopez-Villalobos, N., Dalley, D. E., Clark, D. A. & Holmes, C. W. Yields and persistency of lactation in Friesian and Jersey cows milked once daily. Journal of Dairy Science 89, 2017–2024 (2006).
Wall, E. H. & McFadden, T. B. The milk yield response to Frequent milking in early lactation of dairy cows is locally regulated. Journal of Dairy Science 90, 716–720 (2007).
Bernier-Dodier, P., Delbecchi, L., Wagner, G. F., Talbot, B. G. & Lacasse, P. Effect of milking frequency on lactation persistency and mammary gland remodeling in mid-lactation cows. Journal of Dairy Science 93, 555–564 (2010).
Soberon, F. et al. Effects of increased milking frequency on metabolism and mammary cell proliferation in Holstein dairy cows. Journal of Dairy Science 93, 565–573 (2010).
Stelwagen, K. et al. Reduced milking frequency: Milk production and management implications. Journal of Dairy Science 96, 3401–3413 (2013).
Lyons, N. A., Kerrisk, K. L., Dhand, N. K. & Garcia, S. C. Factors associated with extended milking intervals in a pasture-based automatic milking system. Livestock Science 158, 179–188 (2013).
Murney, R., Stelwagen, K., Wheeler, T. T., Margerison, J. K. & Singh, K. The effects of milking frequency in early lactation on milk yield, mammary cell turnover, and secretory activity in grazing dairy cows. Journal of Dairy Science 98, 305–311 (2015).
Charton, C. et al. Individual responses of dairy cows to a 24-hour milking interval. Journal of Dairy Science 99, 3103–3112 (2016).
Querengässer, J., Geishauser, T., Querengässer, K., Bruckmaier, R. & Fehlings, K. Investigations on milk flow and milk yield from teats with milk flow disorders. Journal of Dairy Science 85, 810–817 (2002).
Stewart, S. et al. Effects of automatic cluster remover settings on average milking duration, milk flow, and milk yield. Journal of Dairy Science 85, 818–823 (2002).
Clarke, T. et al. Milking regimes to shorten milking duration. Journal of Dairy Research 71, 419–426 (2004).
Caja, G., Ayadi, M. & Knight, C. H. Changes in cisternal compartment based on stage of lactation and time since milk ejection in the udder of dairy cows. Journal of Dairy Science 87, 2409–2415 (2004).
Weiss, D., Weinfurtner, M. & Bruckmaier, R. M. Teat anatomy and its relationship with quarter and udder milk flow characteristics in dairy cows. Journal of Dairy Science 87, 3280–3289 (2004).
Tancin, V., Ipema, B., Hogewerf, P. & Macuhova, J. Sources of variation in milk flow characteristics at udder and quarter levels. Journal of Dairy Science 89, 979–988 (2006).
Sandrucci, A., Tamburini, A., Bava, L. & Zucali, M. Factors affecting milk flow traits in dairy cows: Results of a field study. Journal of Dairy Science 90, 1159–1167 (2007).
De Passillé, A. M., Marnet, P.-G., Lapierre, H. & Rushen, J. Effects of twice-daily nursing on milk ejection and milk yield during nursing and milking in dairy cows. Journal of Dairy Science 91, 1416–1422 (2008).
Bruckmaier, R. M. & Wellnitz, O. Induction of milk ejection and milk removal in different production systems. Journal of Animal Science 86, 15–20 (2008).
Bade, R. D., Reinemann, D. J., Zucali, M., Ruegg, P. L. & Thompson, P. D. Interactions of vacuum, b-phase duration, and liner compression on milk flow rates in dairy cows. Journal of Dairy Science 92, 913–921 (2009).
Ambord, S. & Bruckmaier, R. M. Milk flow-dependent vacuum loss in high-line milking systems: Effects on milking characteristics and teat tissue condition. Journal of Dairy Science 93, 3588–3594 (2010).
Berry, D. P., Coughlan, B., Enright, B., Coughlan, S. & Burke, M. Factors associated with milking characteristics in dairy cows. Journal of Dairy Science 96, 5943–5953 (2013).
Edwards, J. P., Jago, J. G. & Lopez-Villalobos, N. Analysis of milking characteristics in New Zealand dairy cows. Journal of Dairy Science 97, 259–269 (2014).
Penry, J. F. et al. Effect of incomplete milking on milk production rate and composition with 2 daily milkings. Journal of Dairy Science 100, 1535–1540 (2017).
Beever, D. E., Rook, A. J., France, J., Dhanoa, M. S. & Gill, M. A review of empirical and mechanistic models of lactational performance by the dairy cow. Livestock Production Science 29, 115–130 (1991).
Pollott, G. E. Deconstructing milk yield and composition during lactation using biologically based lactation models. Journal of Dairy Science 87, 2375–2387 (2004).
Hanigan, M. D., Rius, A. G., Kolver, E. S. & Palliser, C. C. A redefinition of the representation of mammary cells and enzyme activities in a lactating dairy cow model. Journal of Dairy Science 90, 3816–3830 (2007).
Shorten, P. R., Vetharaniam, I., Soboleva, T. K., Wake, G. C. & Davis, S. R. Influence of milking frequency on mammary gland dynamics. Journal of Theoretical Biology 218, 521–530 (2002).
Nielsen, P. P., Pettersson, G., Svennersten-Sjaunja, K. M. & Norell, L. Variation in daily milk yield calculations for dairy cows milked in an automatic milking system. Journal of Dairy Science 93, 1069–1073 (2010).
Klopcic, M., Koops, W. J. & Kuipers, A. A mathematical function to predict daily milk yield of dairy cows in relation to the interval between milkings. Journal of Dairy Science 96, 6084–6090 (2013).
Silvestre, A. M., Martins, A. M., Santos, V. A., Ginja, M. M. & Colaço, J. A. Lactation curves for milk, fat and protein in dairy cows: a full approach. Livestock Science 122, 308–313 (2009).
Gradiz, L., Alvarado, L., Kahi, A. K. & Hirooka, H. Fit of Wood’s function to daily milk records and estimation of environmental and additive and non-additive genetic effects on lactation curve and lactation parameters of crossbred dual purpose cattle. Livestock Science 124, 321–329 (2009).
Pollott, G. E. Do Holstein lactations of varied lengths have different characteristics? Journal of Dairy Science 94, 6173–6180 (2011).
Killick, R. & Eckley, I. A. changepoint: An R Package for Changepoint Analysis. Journal of statistical Software 58(3), 1–19 (2014).
Box, G. E. P. & Pierce, D. A. Distribution of residual correlations in autoregressive-integrated moving average time series models. Journal of the American Statistical Association 65, 1509–1526 (1970).
Acknowledgements
This work was carried out as part of the INRA Rumiflame Metaprogramme. The observed data used in model development were collected at INRA’s Mirecourt Farm. The project would not have been successful without the support of Gwenaël Vourc’h, head of the Animal Epidemiology Research Unit (INRA Clermont-Ferrand-Theix), and the computer skills of Robin Loche and Gaël Nomezine. We wish to thank Joël Chadeuf (MIA Joint Research Unit, INRA Avignon) for his help, constructive criticism, and constant encouragement. We also wish to thank Jessica Pearce for translating the manuscript into English.
Author information
Authors and Affiliations
Contributions
P.G. developed the methodology and the computer model; he also performed the analysis. J.M.T. helped manage the data. Both authors discussed the results and wrote the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare that they have no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Gasqui, P., Trommenschlager, JM. A new standard model for milk yield in dairy cows based on udder physiology at the milking-session level. Sci Rep 7, 8897 (2017). https://doi.org/10.1038/s41598-017-09322-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-017-09322-x
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.