Abstract
Tuberculosis (TB) is a global health problem that affects over 10 million people. There is an urgent need to develop novel antimicrobial therapies to combat TB. To achieve this, a thorough understanding of key validated drug targets is required. The enoyl reductase InhA, responsible for synthesis of essential mycolic acids in the mycobacterial cell wall, is the target for the frontline anti-TB drug isoniazid. To better understand the activity of this protein a series of mutants, targeted to the NADH co-factor binding pocket were created. Residues P193 and W222 comprise a series of hydrophobic residues surrounding the cofactor binding site and mutation of both residues negatively affect InhA function. Construction of an M155A mutant of InhA results in increased affinity for NADH and DD-CoA turnover but with a reduction in Vmax for DD-CoA, impairing overall activity. This suggests that NADH-binding geometry of InhA likely permits long-range interactions between residues in the NADH-binding pocket to facilitate substrate turnover in the DD-CoA binding region of the protein. Understanding the precise details of substrate binding and turnover in InhA and how this may affect protein-protein interactions may facilitate the development of improved inhibitors enabling the development of novel anti-TB drugs.
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Introduction
Tuberculosis (TB) remains a significant global health problem. An estimated 10.4 million people developed TB and 1.4 million died in 2015 as a result of infection with Mycobacterium tuberculosis, the causative agent of TB1. The TB problem is exacerbated by the emergence of multi-drug resistant (MDR) M. tuberculosis, of which there were an estimated 440,000 cases in 20082, 3. There have been few major breakthroughs in TB treatment in the last 50 years4 and as a result there is an urgent need for novel drugs and treatment regimens to combat the spread of the disease.
Mycolic acids are vital, major components of the mycobacterial cell wall and their biosynthesis is a key validated target for many frontline anti-tubercular drugs such as Isoniazid (INH), ethionamide and Isoxyl5, 6. Mycolic acids have also recently been the focus of the development of new anti-tuberculosis drugs such as pyridomycin7. Mycolic acids are long chain α-alkyl β-hydroxyl fatty acids existing in two main forms in the mycobacterial cell; either as unanchored trehalose or glycerol esters, or as anchored mycolic acids, which are linked to the arabinogalactan component of the cell wall, contributing to the unique overall cell wall architecture of the mycobacteria8. In M. tuberculosis, mycolic acids are biosynthesised through the action of two fatty acid synthases, a eukaryotic-like type I and a prokaryotic-like type II enzyme termed FAS I and FAS II respectively. FAS I performs the de novo biosynthesis of C16 and C24–26 acyl-CoAs9. These medium-length acyl-CoAs then serve as primers for the FAS II system, being iteratively condensed through the action of the β-ketoacid-AcpM synthase III (FabH) with the malonyl-Acyl carrier protein (AcpM)10, 11. The second stage of elongation requires the action of the NADPH-dependent-β-ketoacyl-ACP reductase, MabA, resulting in the formation of β-hydroxyacyl-AcpM. The β-hydroxyacyl-AcpM is subsequently dehydrated by the HadABC dehydratases12 and then subsequently reduced by the 2-trans-enoyl-AcpM reductase, InhA13. The successive steps of condensation resulting in the long chain meromycolyl-AcpMs (up toC56), which are the direct precursors of mature mycolic acid, are performed by the β-ketoacyl-AcpM synthases (KasA and KasB)14, 15.
A key enzymatic step in this pathway is the 2-trans-enoyl-AcpM reductase, InhA, which belongs to a family of short-chain reductases13, 16 (Pfam Family PF1356117) and is essential for growth and survival in mycobacteria18.
Recently it has been shown that InhA is controlled post-translationally by phosphorylation19,20,21, suggesting that there is a fine level control over mycolic acid biosynthesis and maturation in mycobacteria. Genetic inactivation of InhA results in the accumulation of the FAS I end product Hexacosanoic acid (C26) and is similar to that observed in INH treated cells18. INH is a pro-drug and in vivo there is a requirement for INH to be activated by the catalase peroxidase, KatG, resulting in the formation of a isonicotinoyl radical that reacts with NAD forming the InhA inhibitory INH-NAD adduct22. Interestingly much of the clinical resistance observed is due to recessive mutations in several genes (katG, ndh, msh, nat) whereas dominant mutations occur in inhA 23. InhA is the primary target of INH, ethionamide and triclosan24, 25, given the demonstration that the transfer of the inhA S94A mutant allele in M. tuberculosis is sufficient to confer resistance to both INH and ethionamide2. Moreover, over-expression of inhA also confers resistance to INH in mycobacteria1, 26.
InhA plays and essential role in the biosynthesis of mycolic acids in M. tuberculosis and remains one of our primary and validated anti-TB drug targets. Given the rise of MDR-TB in the clinic it is important that a full understanding of the catalytic mechanism of this enzyme is obtained. Specifically, the role played by all potential molecular contacts with substrate, cofactors and inhibitors and how these residues contribute to overall enzyme functionality will give us a better understanding of how to design more potent inhibitors of their activity. Here we examine previously unstudied catalytic pocket residues through mutations in terms of their kinetics relating to NADH and DD-CoA substrate binding and turnover. We demonstrate that mutation of NADH-binding pocket residues can have profound effects on DD-CoA binding and suggest that there are long range molecular interactions in the InhA protein.
Results
Structural studies of InhA performed by Rozwarski et al.3, 27, suggest that hydrogen-bonding interactions within the NADH binding pocket of InhA are key to INH inhibitory activity. To investigate this, single point mutations of InhA were made that potentially disrupt key molecular interactions. The S94A mutation is known to confer INH resistance in the clinic and in experimental studies, with the direct role of this residue believed to be via coordination of a water molecule, within a wider hydrogen-bonding network4, 27. The work of Rozwarski et al.5, 6, 27, suggests that M155 interacts indirectly with the nicotinamide ring of NADH or the pyridine ring of the INH-NAD adduct via a coordinated water molecule. The hydrophobic residues P193 and W222 are part of a series of hydrophobic residues that surround the pyridine ring of the isonicotinic acyl group7, 27 (Fig. S1).
To establish the biochemical role of each of these residues, each site was mutagenized (Oligonucleotides are detailed in Table 1) to an alanine residue and the resulting mutant enzymes were assayed for biochemical function with the natural substrates NADH and DD-CoA and the ability to turnover NADH was tested in the presence of the drug adduct INH-NAD and compared to the WT InhA enzyme. All purified mutant proteins were subjected to Circular Dichroism (CD), to evidence correct folding of the mutant proteins. The CD spectra of WT and all mutant proteins exhibited spectra that could be superimposed on each other, indicating that changes to enzyme activity was not due to misfolding or major overall structural changes (data not shown).
Kinetic analysis of InhA and site-directed mutant proteins
An overall kinetic analysis of WT InhA, InhA S94A, InhA M155A, InhA P193A and InhA W222A proteins is summarised in Tables 2 and 3 and Fig. 1. The WT and S94A InhA alleles exhibited similar values to those previously obtained in the literature and served as useful benchmark values with which to understand the role the additional residues that were studied here.
As previously shown8, 16, the S94A mutation has similar activity compared to WT but a reduced NADH affinity (Km), albeit with an increased Vmax (Tables 2 and 3). Here we show that the affinity of InhA S94A for DD-CoA is also decreased, with a Km of 106 µM compared to 75 µM for WT InhA, again however there is also an increase in Vmax (Tables 2 and 3). The InhA S94A mutant kcat NADH and kcat DD-CoA was found to have increased 1746 min−1 and 789 min−1 respectively compared to 1022 min−1 and 694 min−1 for WT InhA. The kcat/Km ratio for NADH indicates that the efficiency of the S94A mutants is 82% of the WT (Table 2: kcat/Km of 8.5 μM−1 min−1 compared to 10.4 μM−1 min−1). The kcat/Km ratio for DD-CoA indicates around 80% efficiency of the S94A mutant compared to the WT InhA.
The activity of an InhA M155A mutant was assessed to gain insight in to its effect on NADH and DD-CoA turnover. Interestingly the Vmax for NADH was equivalent to that of WT, however the M155A mutants Vmax for DD-CoA was around 64% of the WT rate (Fig. 1 and Tables 2 and 3). Overall the M155A mutant had an increased affinity (Km) for NADH (Table 2: 65 μM compared to 98 μM for WT). Moreover, modifying the M155 residue significantly increases the affinity of the mutant for DD-CoA (Table 3: 26 μM compared to 75 μM for WT). kcat of the InhA M155A mutant for NADH was similar to that of WT InhA (Table 2) however the kcat for DD-CoA was around 64% of the WT, suggesting that there may be long range structural interactions within the protein that are negatively affected by mutation of the M155 residue.
Mutation of P193 inactivates the ability of InhA to turn over either NADH and DD-CoA (Fig. 1A and B; Tables 2 and 3), which is consistent with the proposed role of P193 directly interacting with the nicotinamide group. The P193A mutant exhibits a much lower Km for both NADH and DD-CoA, with residual activity of the mutant around 1% of the WT InhA. This also leads to much reduced catalytic efficiency of the InhA P193A mutant with an NADH kcat of 5.8 min−1 compared to 1022 min−1 for WT InhA, which indicates that this mutation is likely to be integral to the pocket geometry and function. The kcat/Km ratio shows that the enzyme efficiency of InhA P193A with NADH is 12% of the WT and enzyme efficiency for DD-CoA is 5% of the WT. This suggests that this proline residue plays a key structural role in the distal area of the NADH binding pocket.
Similar to the role of residue P193, the tryptophan residue 222 is proposed to interact with the nicotinamide group9, 27. Kinetic analysis of the InhA W222A mutant indicates a reduction in overall enoyl reductase activity (Tables 2 and 3; Fig. 1). The W222A mutant showed an increased affinity (Km) for NADH but with a reduced Vmax (Table 2), with a concomitant reduction in kcat when compared to WT InhA. The efficiency of the W222A mutant, when considering the kcat/Km ratio shows that it is around 70% of the WT enzyme for NADH. Conversely the W222A mutation reduces the affinity of InhA for DD-CoA by around two-fold (Table 3), although Vmax is increased along with the kcat for DD-CoA. The kcat/Km ratio shows that the enzyme efficiency of an InhA W222A is around 80% of WT (Table 3). While it is proposed to have a similar interaction with NADH as P19327, W222 may play a role in the allosteric signalling to the DD-CoA binding region of the protein, or through mediating protein-protein interactions.
Inhibition of InhA and mutants with INH-NAD
To test how the mutant derivatives of InhA behave when exposed to the inhibitory drug adduct, the rate of NADH turnover (Vmax) was measured when the proteins were incubated with 100 μM INH-NAD, prior to activity assays with the exception of InhA S94A, which showed no difference in activity from protein incubated in the absence of INH-NAD and was subsequently incubated with 300 μM INH-NAD to demonstrate inhibition (Table 4). These data indicate that inhibition of all mutants with INH-NAD is possible with residual activity following incubation with INH-NAD of 17% for WT InhA, 19% for InhA M155A, 23% for InhA W222A. Incubation of InhA P193A with INH-NAD showed a similar level of activity as the uninhibited protein, which was already shown to be inactive (Table 4).
Discussion
Previous studies of InhA have identified key residues that contribute to the mechanism of action of both the enzyme10, 11, 28, its activation through phosphorylation12, 19 and also contribute to our knowledge of the mechanism of action of the frontline anti-TB drug, INH. The results presented here expand our knowledge of key residues in the active site by adding information to our understanding that the role NADH turnover may play in potential allosteric mediated interactions with DD-CoA and/or the assembly of multimeric states of InhA. Solving the structure of InhA allowed the identification of residues within InhA responsible for binding NADH that may play a key role in binding both natural substrate and the inhibitory drug adduct INH-NAD13, 22, 27. The residues studied here were predicted to make significant molecular contacts with NADH or INH-NAD and are indicated in Fig. S1.
InhA mutations in clinical INH resistant strains are generally located in the NADH binding pocket14,15,16, 29, with a key residue being S94. This study of an S94A mutant confirmed previous data12 that this single residue change results in an increased turnover of the NADH substrate, via an increase in Vmax coupled with a reduced affinity (Km) for NADH and the INH-NAD adduct, however this reduced affinity is not sufficient to explain drug resistance. The data presented here support this hypothesis, indicating that mutations resulting in altered geometry in the NADH binding pocket may have significant effects elsewhere in the protein, with the DD-CoA affinity of the S94A mutant also being altered despite the distance from this region of the protein. Moreover, the M155 and P193 mutations affect DD-CoA turnover and affinity in addition to the local effects observed in the NADH binding pocket. These observations fit with inter- and intramolecular signalling hypotheses of Rawat et al.13, 16, 29. and indicates that further work on interactions with the FASII complex and the molecular dynamics of the protein would be valuable in fully elucidating the mechanism of mycolic acid biosynthesis.
Work by Rawat et al.17, 29, also suggests that there is a two-step mechanism for InhA binding the INH-NAD adduct, where an initial weak binding of the adduct is followed by slow conversion to a tightly inhibited complex. Moreover, these authors suggest that protein-protein interaction, supported by the work of Bloch and others18, 30, 31, between multiple FASII pathway enzymes (including InhA) result in the formation of a functional mycolic acid biosynthetic complex. It is believed that these interactions may modulate the catalytic activity of InhA when a functional tetramer is formed and in a complex with other FASII enzymes gives rise to the appearance of allosteric binding characteristics of InhA kinetics19,20,21, 29, 32.
Intriguingly, an M155A mutation has an apparent decreased Km for NADH and decreased Km for DD-CoA suggesting an overall gain of function mutation via M155. These data show that mutation of a key NADH-binding pocket residue can have profound effects of other regions and activities of the enzyme. This is not simply mediated through binding of a substrate (NADH or INH-NAD) in the NADH-binding pocket as the InhA M155A mutant is inhibited by the INH-NAD drug adduct in a similar manner to WT InhA. These data suggest that the dynamics of catalytic activity and inhibition are subtler than previously thought and may point towards long range interactions within the molecule that are not easily observed through static crystallography approaches.
In summary, mutagenesis of key residues in the NADH-binding pocket of InhA can alter the enzymatic properties of both substrate binding pockets in a subtle manner and suggest that long-range intramolecular interactions may affect substrate turnover and binding for each of the substrates. Currently it is unclear how these mutations may affect assembly of the FASII protein complexes, but further work in these areas will allow deeper insight in to the inhibition of a fundamental cellular process for M. tuberculosis and may lead to novel therapeutics.
Materials and Methods
Bacterial strains and plasmids – Escherichia coli
DH5α (Invitrogen) was used for standard cloning/transformation and E. coli BL21 (DE3) Star (Novagen) was used for protein expression.
Site-directed mutagenesis, expression and purification of recombinant InhA and mutant proteins
InhA was expressed from the plasmid pETPhos_inhAWT (N-terminal Histidine tagged). Site-Directed Mutants (SDMs) were constructed using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) according to the manufacturer’s instructions. SDM oligonucleotides are detailed in Table 1. All SDM plasmids were confirmed by sequencing. BL21 (DE3) Star freshly transformed with Wild-Type or mutant InhA plasmids were grown in LB medium with 100 μg ml−1 carbenicillin (Melford labs) overnight at 37 °C. These overnight cultures were used to inoculate 500 ml of LB and were grown under the same conditions, with shaking until they reach an A600 = 0.5. Isopropyl-thio-β-D-galactopyranoside (IPTG) was added to a final concentration of 1 mM and incubation was continued for 3 h at 37 °C. Cells were collected by centrifugation (4000 × g, 10 mins), washed and resuspended in Buffer A (20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% Glycerol, and 20 mM imidazole) with Complete EDTA-Free Protease inhibitor cocktail (Roche) and Benzonase (Sigma-Aldrich). Cells were lysed using BugBuster (Novagen) according to the manufacturer’s instructions and lysates were clarified by centrifugation (4000 × g, 10 mins). InhA was purified by Ni2+-affinity chromatography (His-Trap, GE Healthcare) using an AKTA chromatography system. InhA was eluted from the column using Buffer B (20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% Glycerol and 1 M imidazole). Fractions containing pure InhA (with the histidine-tag still intact, but has no effect on activity18, 28) were pooled, dialysed to remove imidazole and concentrated as required and stored in 20% glycerol at −20 °C as required. All data reported are the result of three technical replicates generated from three independent biological (independent over-expression) replicates.
Assaying InhA enzymatic activity
The enoyl reductase activity of InhA was assayed spectrophotometrically following the oxidation of NADH to NAD+ at 340 nm. All measurements (unless stated) were performed using a Shimadzu UV-2550 dual-beam absorption spectrometer (Shimadzu Scientific Instruments). Reactions were carried out at 37 °C in 100 mM phosphate buffer, pH7.5 containing 1.47 µM of InhA (Wild-type or mutant protein) and varying levels of NADH and the trans−2-Dodecenoyl-Coenzyme A (DD-CoA) substrate. The data all activity measurements were single cuvette measurements run in triplicate over a total duration of 300 seconds. The DD-CoA substrate was synthesised from 2-Dodecenoic acid (Obtained from Enamine Ltd) using the anhydride method of Quémard et al.13, 22. The INH-NAD adduct was synthesised in the presence of InhA, producing inhibited InhA according to the method of Rozwarski et al.22, 23, where 350 μM WT or mutant InhA was incubated in 50 mM HEPES buffer (pH 7.5) with 17.5 mM NADH, 35 mM INH and 3.5 mM MnCl2. Inhibition assays for all alleles were conducted by removing aliquots from the incubation mixture previously described and spectrophotometrically monitoring the turnover of NADH to NAD+ at 340 nm in the presence of DD-CoA in the same manner as for the activity assays. All control reactions were collected simultaneously consisting of a fixed 100 µM concentration of NADH and the appropriate amount of DD-CoA but in the absence of enzyme. The kinetic parameters for all assays were determined at a fixed, saturating concentration of either NADH or DD-CoA (100 μM and 50 μM respectively), whilst varying the substrate or cofactor accordingly. Thus allowing the kinetics of inhibited WT InhA and mutant InhA to be determined under appropriate conditions. Values for Km and Vmax for each allele were calculated from a plot of a series of v0 values determined experimentally by varying one substrate concentration whilst the holding other at a fixed value. Values for Km and Vmax initially as a function of [NADH] and then [DD-CoA] are obtained directly by non-linear regression fitting of the Michaelis-Menten equation,
(where [substrate] represents [DD-CoA] or [NADH]) to the data. The equation was incorporated as a user-defined fitting function into the curve fitting analysis suite within the Igor Pro 6.37 [Wavemetrics] software which was used in all data/statistical analysis procedures. The fitting procedure utilises the Levenberg-Marquardt algorithm or Nonlinear Least-Squares method which returns estimates for Km and Vmax along with the standard error (±2σ) taken as the uncertainty.
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Acknowledgements
Funding from The Leverhulme Trust (RPG248) for this work is gratefully acknowledged by PAH and NTH. We would also like to acknowledge the support of the Biochemical Society for funding vacation studentship to BVV.
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P.A.H. and N.T.H. conceived the study; D.S., V.M., K.R., B.V., M.T. conducted the experimental work; P.A.H., N.T.H. and D.S. wrote-up the work. All authors have given approval to the final version of the manuscript.
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Shaw, D.J., Robb, K., Vetter, B.V. et al. Disruption of key NADH-binding pocket residues of the Mycobacterium tuberculosis InhA affects DD-CoA binding ability. Sci Rep 7, 4714 (2017). https://doi.org/10.1038/s41598-017-05042-4
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DOI: https://doi.org/10.1038/s41598-017-05042-4
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