Hydrogen sulfide (H2S) has been recognized as a signalling molecule which affects the activity of ion channels and transporters in epithelial cells. The cystic fibrosis transmembrane conductance regulator (CFTR) is an epithelial anion channel and a key regulator of electrolyte and fluid homeostasis. In this study, we investigated the regulation of CFTR by H2S. Human CFTR was heterologously expressed in Xenopus oocytes and its activity was electrophysiologically measured by microelectrode recordings. The H2S-forming sulphur salt Na2S as well as the slow-releasing H2S-liberating compound GYY4137 increased transmembrane currents of CFTR-expressing oocytes. Na2S had no effect on native, non-injected oocytes. The effect of Na2S was blocked by the CFTR inhibitor CFTR_inh172, the adenylyl cyclase inhibitor MDL 12330A, and the protein kinase A antagonist cAMPS-Rp. Na2S potentiated CFTR stimulation by forskolin, but not that by IBMX. Na2S enhanced CFTR stimulation by membrane-permeable 8Br-cAMP under inhibition of adenylyl cyclase-mediated cAMP production by MDL 12330A. These data indicate that H2S activates CFTR in Xenopus oocytes by inhibiting phosphodiesterase activity and subsequent stimulation of CFTR by cAMP-dependent protein kinase A. In epithelia, an increased CFTR activity may correspond to a pro-secretory response to H2S which may be endogenously produced by the epithelium or H2S-generating microflora.
The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride and bicarbonate conducting anion channel which is found in many vertebrate epithelia and essential for the epithelial regulation of electrolyte and fluid homeostasis. Gene mutations in CFTR cause cystic fibrosis, the most common autosomal-recessive disorder in Caucasians, with a disease incidence of around 1 in 1000–3000 in northern Europeans1. The CFTR protein contains two membrane-spanning regions (each consisting of six transmembrane domains) functioning as the channel pore, which are connected to two intracellular nucleotide binding domains (NBD1 and NBD2) as well as a regulatory (R) domain2. NBD1 and NBD2 regulate the opening and closing of the channel by binding and hydrolysing ATP2,3, whereas the R-domain initiates the transitions in channel conformation by protein kinase A (PKA)-dependent phosphorylation2. Although the NBDs and R-domain contain various phosphorylation sites which control biogenesis, trafficking, interaction with other proteins and channel open probability2, PKA is the primary regulator of CFTR activity. At the cellular level, CFTR is hence mainly regulated by cAMP-coupled signalling events.
CFTR is located in the apical membrane of epithelial cells, where it primarily represents a conductance for chloride ions and facilitates transepithelial movement of chloride4. CFTR allows chloride permeation in and out of the cells and the direction of chloride flux is determined by the gradient for chloride ions between the cytoplasm and luminal extracellular fluid, as well as the apical membrane potential of the epithelial cells5. Whereas CFTR mediates chloride secretion in the intestine, pancreas, secretory coils of sweat glands or serous cells of airway submucosal glands4,6, it absorbs chloride in the ducts of sweat glands4 or, as recently suggested, in the airway surface epithelium7. Its physiological importance is also reflected in the consequence of CFTR malfunction and cystic fibrosis phenotype which includes pancreas insufficiency, airway mucus obstruction, meconium ileus and high sweat chloride concentrations1. Under physiological conditions, multiple cellular signalling cascades regulate the activity or membrane abundance of CFTR8, thus allowing for a precise regulation of chloride flux and, eventually, electrolyte and fluid homeostasis.
Hydrogen sulfide (H2S) is a well-known environmental chemical threat with a characteristic odour of rotten eggs; however, research over the past decade has revealed that H2S is also an important cellular signalling molecule9. H2S is involved in a variety of physiological and patho-physiological processes (for review see ref.9), and H2S-liberating compounds are currently explored for a therapeutic potential10. Physiological concentrations of H2S are likely in the nano- to low micro-molar range and depend on its production, intracellular storage and mitochondrial degradation11. H2S is enzymatically generated within the metabolism of L-cysteine by cystathionine-γ-lyase, cystathionine-β-synthase or 3-mercaptopyruvate sulfurtransferase. Enzymatically generated H2S can either be stored as sulfane sulfur (oxidative formation of protein polysulfides)12,13; or degraded by the sulfide oxidation pathway in mitochondria14.
In addition to enzymatically generated H2S, there are exogenous sources of this gas15. For example, up to millimolar amounts of H2S can occur in the digestive tract due to food content and the activity of sulphur-metabolising microbiota16. Epithelia are predominantly exposed to exogenous H2S of environmental or microbiological origin and are challenged to maintain low, physiological H2S concentrations in order to prevent potential toxicity due to high levels of exogenous H2S15,17. In addition to a high H2S metabolising capacity in epithelial cells17, we recently hypothesised that epithelia use their electrolyte and liquid secreting machinery as a defence strategy in order to flush potentially harmful sources for H2S from the epithelial surfaces15.
In epithelia, H2S exerts pro-secretory effects by either enhancing the secretion, or decreasing the absorption of electrolytes and liquid across the epithelium15. In rat, guinea pig and human colon preparations, H2S stimulates the secretion of chloride15. This is either due to direct actions on ion channels and transporters within the epithelial cells18,19, or indirectly by stimulating enteric secretomotor neurons20. In rat colon epithelial cells, H2S induces an increase in the intracellular calcium concentration, which triggers the opening of apical calcium-sensitive chloride channels as well as calcium- and ATP-sensitive potassium channels in the basolateral epithelial cell membrane. This hyperpolarises the apical membrane potential and facilitates apical chloride efflux18,19. Similar observations were recently reported in a rat vaginal epithelial preparation, where exogenous H2S elicits a chloride secretion which involved activation of basolateral ATP-sensitive potassium channels and apical chloride efflux via CFTR21. However, the mechanism of how H2S might facilitate opening of CFTR remains elusive.
In this study we investigated the regulation of CFTR by H2S. Using heterologous expression of human CFTR in Xenopus oocytes, we provide evidence that low-micromolar H2S concentrations decrease phosphodiesterase activity. This activates the cAMP/PKA signalling axis and triggers activation of CFTR.
Hydrogen sulfide stimulates CFTR in Xenopus oocytes
Human CFTR was heterologously expressed in Xenopus laevis oocytes and channel activity was measured electrophysiologically by two-electrode voltage-clamp (TEVC) microelectrode recordings. In order to investigate the potential influence of H2S on heterologously expressed CFTR, the H2S-forming sulphur salt Na2S was employed (Fig. 1). The application of 50 µM Na2S to CFTR-expressing oocytes significantly increased transmembrane currents from −0.059 ± 0.015 µA to −0.395 ± 0.11 µA (n = 6; N = 3; P = 0.0313; Fig. 1a,b). This effect was transient, as transmembrane currents began to decline in the presence of Na2S. Furthermore, the effect was fully reversible upon removal of Na2S. In order to confirm expression of CFTR, the cAMP-elevating compounds forskolin (5 µM) and IBMX (100 µM) were applied to the oocyte’s superfusate. The application of forskolin/IBMX elicited a significant increase in transmembrane current from −0.076 ± 0.015 µA to −1.055 ± 0.186 µA (n = 6; N = 3; P = 0.0030; Fig. 1a,b). Native oocytes which did not express CFTR did not respond to forskolin/IBMX (Fig. 1c). Transmembrane currents of these oocytes were −0.054 ± 0.026 µA before, and −0.056 ± 0.028 µA after application of the drugs (n = 8; N = 3; P = 0.7525). There was also no effect of Na2S (50 µM) on native oocytes. Transmembrane currents were −0.058 ± 0.021 µA without and −0.057 ± 0.023 µA with Na2S (n = 8; N = 3; P = 0.4375; Fig. 1c). However, there was a small but significant increase in transmembrane current from −0.027 ± 0.005 µA to −0.035 ± 0.006 µA when a combination of Na2S and forskolin/IBMX was aspplied (n = 10; N = 2; P = 0.008; Fig. 1c).
The Na2S-induced current of CFTR-expressing oocytes was rapidly inhibited by the additional application of the CFTR inhibitor CFTR_inh-172 (Fig. 1d,e). Under perfusion with 50 µM Na2S, transmembrane currents increased to −0.757 ± 0.145 µA and were rapidly inhibited to −0.153 ± 0.039 µA after addition of 25 µM CFTR_inh-172 (n = 8; N = 2; P = 0.0078). The transient nature of the current which was stimulated by Na2S was not the result of a rapid evaporative loss of H2S from the buffer solutions. Na2S was measured in the employed buffer solution by the formation of methylene blue and detection of its absorbance at 670 nm (Fig. 1f). There was only a minor decrease in methylene blue concentrations over time, indicating that H2S was present in the buffers even after a time period where current signals began to decline.
In order to confirm that the observed activation of CFTR by Na2S was due to H2S, the H2S-releasing compound GYY4137 which is chemically different from a sulphur salt22 was employed. Since GYY4137 is a slow-releasing H2S donor22, higher concentrations (500 µM) were used (Fig. 1g,h). GYY4137 elicited a small but significant activation of CFTR. Transmembrane currents of CFTR expressing oocytes significantly increased from −0.046 ± 0.010 µA to −0.119 ± 0.020 µA (n = 7; N = 2; P = 0.0156) due to application of GYY4137 (Fig. 1g,h).
In sum, these data indicate that H2S activates human CFTR which is heterologously expressed in Xenopus oocytes.
Hydrogen sulfide stimulates CFTR activity via cAMP-mediated signalling events
The classical intracellular signalling cascade activating CFTR involves adenylyl cyclase (AC)-mediated production of cAMP and subsequent activation of protein kinase A (PKA). PKA phosphorylates the regulatory domain of CFTR and activates the channel in the presence of ATP. In order to investigate whether or not H2S interferes with this signalling pathway, the effect of the cAMP-elevating drugs forskolin/IBMX was evaluated with or without H2S.
The application of forskolin/IBMX elicited a significant and transient increase in transmembrane current (Fig. 2a) from −0.064 ± 0.009 µA to −1.364 ± 0.284 µA (n = 11; N = 5; P = 0.0009). This effect was fully reversible upon removal of the drugs. A second application of forskolin/IBMX again stimulated CFTR activity from −0.081 ± 0.022 µA to −0.604 ± 0.114 A (n = 11; N = 5; P = 0.0038; Fig. 2a). The second effect of forskolin/IBMX was normalised to the effect of the first forskolin/IBMX application and defined as ‘normalised CFTR activity’. Without any additional treatment, control oocytes had thus a normalised CFTR activity of 0.42 ± 0.04 (n = 11; N = 5). We then applied increasing concentrations of Na2S after the first application of forskolin/IBMX (Fig. 2a). Interestingly, 50 µM Na2S which elicited robust currents in previous experiments (Fig. 1) did not significantly stimulate transmembrane currents after the oocytes had been exposed to forskolin/IBMX (Fig. 2a,c). Only a high dose of 300 µM Na2S triggered a small increase in transmembrane currents from −0.033 ± 0.010 µA to −0.051 ± 0.009 µA (n = 8; N = 3; P = 0.0423; Fig. 2a,c). However, despite the lack of an effect of Na2S after previous exposure of the oocytes to forskolin/IBMX, Na2S enhanced the second effect of forskolin/IBMX. Normalised CFTR activity dose-dependently increased due to application of Na2S (Fig. 2a,d). This effect was inhibited by 25 µM of CFTR_inh.172 (Fig. 2b,d). Furthermore, there was only a minor current activation due to 50 µM Na2S and forskolin/IBMX in native, non-CFTR expressing oocytes (Fig. 1c). These data suggest that Na2S potentiates CFTR-activity which was elicited by forskolin/IBMX.
To investigate if the Na2S-induced stimulation of CFTR involves AC and PKA, specific inhibitors of these enzymes were employed and Na2S-induced currents (INa2S) were estimated with or without these drugs (Fig. 3). Na2S (50 µM) was applied twice to CFTR-expressing oocyte in order to control for a potential desensitisation in response to repetitive Na2S-exposure (Fig. 3a,b). The first INa2S was 0.321 ± 0.108 µA and not significantly different from the second INa2S which was 0.312 ± 0.087 µA (Fig. 3b; n = 6; N = 5; P = 0.854).
MDL 12330 A was used as an inhibitor of AC. In control experiments, Na2S (50 µM) was applied to CFTR-expressing oocytes, which led to an INa2S of 0.130 ± 0.040 µA (n = 6; N = 2). Afterwards, the oocytes were perfused with DMSO (0.1%; the solvent for MDL 12330A) and stimulated again with 50 µM Na2S. This resulted in an INa2S of 0.085 ± 0.019 µA (n = 6; N = 2), which was not significantly different (P = 0.1395) from the first INa2S (Fig. 3c,d). By contrast, when MDL 12330A was applied, INa2S significantly decreased from 0.133 ± 0.035 µA to 0.011 ± 0.003 µA (n = 8; N = 2; P = 0.0056; Fig. 3c,d).
A similar experiment was performed with cAMPS-Rp – a PKA antagonist – which was directly injected into the oocytes during experiments. For control experiments, CFTR-expressing oocytes were stimulated with Na2S (50 µM). Subsequently, 9.2 nl of an intracellular-analogous solution (IAS) was injected into the oocytes and the cells were stimulated a second time with Na2S. This manoeuvre increased (although values did not reach statistical significance) INa2S from 0.255 ± 0.046 µA to 0.484 ± 0.126 µA (n = 9; N = 2; P = 0.0743; Fig. 3e,f). A similar observation has been reported in a previous study23, where a volume-increase in oocytes increased the activation of CFTR by cAMP-elevating compounds. By contrast, injection of the PKA antagonist cAMPS-Rp abrogated the second effect of Na2S. Values of INa2S significantly decreased from 0.248 ± 0.061 µA to 0.011 ± 0.006 µA (n = 9; N = 2; P = 0.0039; Fig. 3e,f). Taken together, these data show that H2S activates CFTR by cAMP- and PKA-mediated signalling in Xenopus oocytes.
Hydrogen sulfide targets phosphodiesterase rather than adenylyl cyclase
An increase in intracellular cAMP concentrations could either be the result of enhanced cAMP production by AC, or inhibition of cAMP degradation by phosphodiesterase (PDE). H2S might thus stimulate AC or inhibit PDE – both effects would result in accumulation of cAMP, a downstream activation of PKA and subsequent stimulation of CFTR. In order to discriminate between AC- and PDE-mediated contributions to CFTR activation, CFTR was stimulated with maximal effective concentrations of either forskolin (AC activator) or IBMX (PDE inhibitor). If H2S potentiated the effect of forskolin, but not that of IBMX, H2S likely prevents cAMP degradation by PDE. If H2S potentiated the effect of IBMX, but not that of forskolin, H2S likely stimulates cAMP production by AC.
First, we investigated if H2S affects the effect of forskolin alone (Fig. 4a). Since we were not able to additionally stimulate CFTR activity by increasing the forskolin concentration to 30 µM (data not shown), we considered the employed concentration of 5 µM as maximally effective, an observation which is consistent with a reported EC50 value of ~0.07 µM for forskolin in airway epithelia24. In control experiments, forskolin (5 µM) was applied to CFTR-expressing oocytes, which stimulated IM by 0.324 ± 0.056 µA (n = 13; N = 3). After wash-out, the oocytes were stimulated again with 5 µM forskolin. This resulted in a second stimulation of IM by 0.363 ± 0.068 µA (n = 13; N = 3; Fig. 4a). The second effect of forskolin was normalised to the effect of the first forskolin application and defined as ‘normalised forskolin effect’ (Fig. 4b). Under these control conditions, the normalised forskolin effect was 1.135 ± 0.146 (n = 13; N = 2). By contrast, oocytes which were treated with 50 µM Na2S (together with the second application of forskolin) had a significantly enhanced normalised forskolin effect of 3.054 ± 0.405 (n = 13, N = 3, P < 0.0001, Gaussian approximation; Fig. 4a,b).
An identical protocol was employed with a high concentration of the PDE inhibitor IBMX (1 mM) and a ‘normalised IBMX effect’ was estimated (Fig. 4c,d). The normalised IBMX effect was 0.714 ± 0.080 (n = 10; N = 3) under control conditions, and not significantly different from that which was estimated in the presence of Na2S which was 0.966 ± 0.178 (n = 10; N = 3; P = 0.2208). Na2S thus enhanced the effect of the AC-agonist forskolin, but not that of the PDE-inhibitor IBMX. H2S might therefore impair PDE-mediated cAMP degradation rather than AC-mediated cAMP production.
In order to confirm these observations a different strategy was employed (Fig. 4e–g). AC-mediated cAMP-production was blocked by application of the AC inhibitor MDL 12330 A (20 µM) to CFTR-expressing oocytes. Subsequently, 100 µM of membrane-permeable 8-Br-cAMP was applied. This stimulated an increase in transmembrane current (IcAMP) of 0.056 ± 0.0221 µA (n = 7; N = 4). After washout of all drugs, MDL 12330 A (20 µM) was applied again and 50 µM Na2S was added. Afterwards, 8Br-cAMP was additionally applied and IcAMP significantly increased to 0.590 ± 0.154 µA (n = 7; N = 4; P = 0.0124; Fig. 4e,g). By contrast, there was no difference between the first and second IcAMP (0.091 ± 0.026 µA and 0.064 ± 0.016 µA; n = 5; N = 2; P = 0.1561) when the procedure was repeated without Na2S (Fig. 4f,g).These data indicate that H2S enhances the efficacy of 8-Br-cAMP.
In sum, these data provide evidence that H2S inhibits endogenous PDE in Xenopus oocytes. This results in cAMP-mediated stimulation of CFTR-activity via downstream activation by PKA.
In this study we investigated the regulation of CFTR by H2S. Previous studies using the mouse hippocampal cell line HT2225 or rat vaginal epithelial preparations21 suggested that CFTR might be a target for H2S. In order to elaborate on this hypothesis, human CFTR was heterologously expressed in Xenopus oocytes and functional CFTR expression was confirmed by application of the cAMP-elevating compounds forskolin and IBMX, which resulted in a transient increase in transmembrane currents which did not occur in native, non-injected oocytes. These observations are consistent with previously published functional electrophysiological analyses of human CFTR in Xenopus oocytes23,26. The H2S-liberating sulphur salt Na2S elicited a transient current stimulation of CFTR-expressing oocytes which was readily inhibited by the CFTR inhibitor CFTR_inh172 and did not occur in native oocytes. Furthermore, the slow-releasing H2S-liberating molecule GYY4137 also stimulated transmembrane currents in Xenopus oocytes. These data indicate that H2S, released from Na2S or GYY4137, stimulates CFTR activity.
We then elaborated on the signalling mechanisms which mediate the H2S-induced activation of CFTR. We first stimulated CFTR-expressing oocytes with forskolin/IBMX and after removal of these drugs, cells were exposed to H2S. Interestingly, a direct response to H2S only occurred when high concentrations of Na2S were employed. When forskolin/IBMX were applied twice to CFTR-expressing oocytes, the second activation by forskolin/IBMX was ~60% smaller than the first one. This is consistent with previous reports23 and can be explained by a desensitisation in response to compounds which lead to a strong increase in intracellular cAMP26. The lack of a pronounced effect of H2S after initial stimulation with forskolin/IBMX is likely explained by the fact that Na2S alone is a much weaker stimulator of CFTR activity than forskolin/IBMX (as shown in Fig. 1a) and its effect is lost within the desensitisation after exposure to these compounds. Nevertheless, we found that H2S potentiated the second effect of forskolin and IBMX, indicating that the effect of H2S is additive to that of forskolin/IBMX. Furthermore, the H2S-induced activation of CFTR was lost in the presence of the AC inhibitor MDL12330A as well as the PKA antagonist cAMPS-Rp. These data indicate that H2S activates CFTR via the cAMP/PKA signalling axis, a finding which is consistent with a previous study demonstrating an increase in intracellular cAMP concentrations by H2S in Xenopus oocytes27. H2S is membrane-permeable28 and might target AC in order to stimulate cAMP production. It has been previously shown that H2S either stimulates27,29 or inhibits30,31 AC activity, indicating that this enzyme represents indeed a molecular target for H2S. However, we found that H2S was able to potentiate the stimulation of CFTR by the AC-activator forskolin, but not by a high concentration (1 mM) of the PDE inhibitor IBMX. If H2S was acting on AC, there should be an additional effect over that of PDE inhibition alone – irrespective of the concentration of the PDE inhibitor. These data therefore suggest that H2S targets degradation rather than production of cAMP. However, these experiments alone do not justify a definite conclusion on the target for H2S. Based on the experiment using forskolin alone, a potential effect of H2S on AC cannot be ruled out completely. Forskolin increases the affinitiy of two cytoplasmic domains (C1 and C2) of AC for each other and enhances its activity32. In the presence of forskolin, AC becomes more sensitive to e.g. Gsα32, suggesting that it is possible that there is a synergistic activation of AC by H2S in the presence of forskolin, but not in the presence of IBMX.
In addition to inhibiting PDEs, IBMX inhibits adenosine receptors, including endogenous adenosine receptors in Xenopus oocytes. Activation of these receptors inhibits AC and antagonising the receptor with IBMX might therefore elevate cAMP-concentrations – irrespective of the block of PDE-mediated degradation of cAMP. However, as shown in a study by Kobayashi et al. the receptors need to be activated by a ligand (adenosine) in order to generate a current signal in oocytes and this does not occur with a perfusion system as employed in our study33. Furthermore, it is not possible to discriminate between cGMP- and cAMP-PDEs using IBMX. It is possible that cGMP activates CFTR in Xenopus oocytes34, however, if H2S acted via cGMP, the effects of Na2S should not be sensitive to the adenylyl cyclase inhibitor MDL. This is further confirmed by the fact that H2S potentiated the stimulation of CFTR by exogenous membrane-permeable 8-Br-cAMP under conditions where endogenous cAMP production was blocked by the AC inhibitor MDL 12330A. The increased CFTR stimulation by 8-Br-cAMP in the presence of H2S can only be explained by impaired degradation of 8-Br-cAMP by endogenous PDE activity. Consistent with this hypothesis, several studies demonstrated that H2S is able to inhibit cAMP and cGMP degradation by PDEs35,36,37.
Most interestingly, small amounts (nano- to lower micro-molar range) of the sulphur salt NaHS inhibited PDE-mediated cAMP breakdown in cell-free systems35,37, suggesting that H2S might directly interfere with PDE activity – independently of cellular signalling cascades. This might be the result of an interference of H2S with zinc, thereby reducing the activity of zinc-dependent PDE35. Alternatively, H2S might target cysteine residues in the enzyme by persulfidation35. However, H2S alone cannot modify thiol groups38, whereas derivatives of H2S such as polysulfides13 or nitroxyl, which might be formed in the cytoplasm of cells, are able to do so38. The precise mechanisms how H2S regulates PDE function remain to be elucidated; however, the Xenopus oocyte might be a useful model in order to address these questions.
In sum, we provide evidence that H2S inhibits endogenous PDE in the Xenopus oocyte, which likely results in an accumulation of cAMP and downstream activation of CFTR via PKA. This scenario, however, requires a constitutive production of cAMP in these cells. Xenopus oocytes are arrested in the G2 stage of meiosis I39. The G2 arrest is maintained by AC-mediated production of cAMP which is believed to prevent oocyte maturation via PKA-mediated signalling events39,40,41. Hence, there is a constitutive production of cAMP in Xenopus oocytes. Furthermore, there is an endogenous PDE activity in these cells42 and the activities of both AC as well as PDE determine the concentration of cAMP. When Xenopus oocytes are exposed to progesterone, cAMP levels decrease, the cells undergo nuclear membrane breakdown (GVBD) and mature into a fertilisable egg39. This process is inhibited by IBMX42, again demonstrating that inhibition of PDE activity leads to an increase in the concentration of cAMP. Consistently, IBMX as well as H2S alone were able to stimulate CFTR activity in these cells in the present study.
Our data are consistent with an emerging body of evidence that H2S targets the cAMP-signalling axis. Whereas this study and others27,29 provide evidence for an activation of the cAMP-pathway by H2S, inhibition of cAMP-signalling has also been reported43,44,45. A study by Lu et al. demonstrated inhibition of AC and stimulation of PDE by NaHS in AS4.1 cells44. These inconsistent findings suggest that the net-effect of H2S on cAMP signalling depends not only on whether AC or PDE is targeted by H2S, but also on the precise molecular repertoire of the cAMP-regulating machinery. In humans, ten isoforms of AC have been identified and there are 11 members of the PDE protein family which can generate nearly 100 different subtypes46. The specific isoform expression in a cell might critically determine whether H2S activates or inhibits cAMP-signalling. This is especially important, since H2S will diffuse across cell membranes in an unspecific manner and hence specificity is likely not achieved by membrane receptors but possibly by the subtype repertoire of intracellular signalling molecules.
In epithelia, H2S exerts pro-secretory or anti-absorptive effects15,47 and we recently suggested a concept by which epithelia use their electrolyte and liquid transport machinery as a defence mechanism in order to flush potential sources for harmful amounts of H2S from the epithelial surfaces15. The herein reported cAMP-mediated stimulation of CFTR activity would be consistent with a pro-secretory action on chloride-secreting epithelia. A recent study demonstrated a CFTR-mediated chloride secretion across rat vaginal epithelial preparations21. The authors speculated that this might be due to an increase in cAMP21 and our findings would support this hypothesis. Since H2S is not directly targeting CFTR, the PDE repertoire of epithelial cells will determine whether or not H2S triggers CFTR-mediated electrolyte secretion.
Aside from the pro-secretory effects, H2S prevents liquid absorption by sodium-transporting epithelia48,49,50. In these epithelia, cAMP/PKA signalling stimulates sodium absorption by an increase in the membrane abundance of sodium transporting molecules such as the epithelial sodium channel (ENaC)51,52. Interestingly, we recently showed that H2S prevents this cAMP-mediated increase in ENaC abundance in sodium-absorbing lung epithelial cells by yet unidentified mechanisms50. Furthermore, H2S did not enhance cAMP-mediated chloride secretion in primarily sodium absorbing pig airway surface epithelia (data not shown). Nevertheless, this illustrates that – depending on the specific enzymatic repertoire of the cAMP signalling axis – H2S might trigger cAMP-mediated electrolyte secretion in a fraction of epithelial cells, whilst simultaneously preventing enhanced electrolyte absorption in other cells. The herein reported data thus provide a step further in understanding the mechanisms of how H2S elicits a switch from absorptive to secretory electrolyte and water transport in epithelia.
Isolation of Xenopus laevis oocytes
All animal experiments were performed in accordance with the German animal welfare law and had been declared to the Animal Welfare Officer of the University (Registration No.: M_ 478 and M_549). The animal housing facility was licensed by the local authorities (Az: FD 62 - §11 JLU Tierphysiologie). The methods used to euthanize the animals humanely were consistent with the recommendations of the AVMA Guidelines for the Euthanasia of Animals. All procedures and experimental protocols were approved by the Animal Welfare Officer of the University as well as the regional council of Giessen (Registration No.: M_ 478 and M_549).
Oocytes of stages V/VI were isolated from freshly dissected Xenopus laevis ovaries and defolliculated exactly as previously described53. Isolated oocytes were stored at 16 °C in an oocyte culture solution containing 90 mM NaCl, 1 mM KCl, 2 mM CaCl2, 5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 2.5 mM sodium pyruvate, 0.06 mM penicillin G and 0.02 mM streptomycin sulfate at pH 7.4.
CFTR-cRNA synthesis and injection into oocytes
The plasmid construct for human CFTR (in pGEM-HE) was a kind gift from Professor Blanche Schwappach (University of Göttingen, Germany). Plasmids were linearised with Mlu1 (Promega, Mannheim, Germany) and subsequently in vitro transcribed with the RiboMAX Large Scale RNA Production System (Promega) using T7 RNA polymerase. CFTR-cRNA was diluted with diethyl pyrocarbonate (DEPC)-treated water to a final concentration of 250 ng/µl. Fifty-one nanoliters of CFTR-cRNA were injected into oocytes with a Nanoliter-Injector (Drummond Scientific, Broomall, Pennsylvania, USA) yielding final concentrations of 12.5 ng RNA/oocyte. Injected oocytes were cultured for 2–5 days at 16 °C in the oocyte culture solution.
Microelectrode recordings (Two-Electrode Voltage-Clamp, TEVC)
CFTR-expressing oocytes were placed in a Lucite chamber which was continuously perfused with oocyte ringer solution (ORS) containing 90 mM NaCl, 1 mM KCl, 2 mM CaCl2, 5 mM HEPES at pH 7.4. Chlorinated silver wires served as recording electrodes and were mounted into borosilicate glass capillaries (Hilgenberg, Malsfeld, Germany) with an outer diameter of 1.2 mm, which were pulled to microelectrodes with a DMZ universal puller (Zeitz-Instruments, Martinsried, Germany) and filled with 1 M KCl. Ag/AgCl wires were used as reference electrodes and placed directly into the recording chamber. The membrane voltage was clamped to −60 mV using a TEVC amplifier (Warner Instruments, Hamden, Connecticut, USA). Transmembrane currents (IM) were low-pass filtered at 1000 Hertz (Frequency Devices 902, Haverhill, Massachusetts, USA) and continuously recorded with a strip chart recorder (Kipp&Zonen, Delft, The Netherlands).
Determination of evaporative loss of H2S from buffer solutions
The equilibration of H2S with its concentration in air will eventually lead to evaporative loss of this gas from the experimental buffer solutions54. Therefore the relative concentration of H2S in ORS was determined at various time points after administration of 50 µM Na2S by the formation of methylene blue. Samples (300 µl) of the solutions were mixed with 500 µl of 4% zinc acetate and incubated on ice for at least 30 min. Afterwards, 200 µl of 0.1% dimethylphenylendiamine sulfate (in 5 M HCl) and 100 µl of 50 mM FeCl3 were added. Samples were vortexed, centrifuged at 5000× g and incubated for 5 min at room temperature. The absorption of methylene blue was measured at 670 nm with a Vis-spectrophotometer (Kruess Optronic, Hamburg, Germany).
Chemicals and solutions
In order to apply H2S, the sulfur salt Na2S (Sigma, Taufkirchen, Germany) or the slow releasing H2S donor GYY4137 (Santa Cruz, Biotechnology, Dallas, Texas, USA) were employed. Na2S was prepared as a stock solution of 50 mM in ORS freshly before experiments and immediately diluted to final working concentrations in order to prevent evaporative loss of H2S from the experimental solutions. Stock solutions of 100 mM GYY4137 were prepared in H2O and stored at −20 °C. Forskolin (MoBiTec, Göttingen, Germany) was used as a stimulator of adenylyl cyclase and stock solutions of 10 mM were prepared in dimethyl sulfoxide (DMSO, Sigma) and stored at −20 °C. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX; Sigma, Taufkirchen, Germany) was dissolved to 100 mM in DMSO and stored at +4 °C. The adenylyl cyclase inhibitor MDL 12330 A hydrochloride (MDL; Tocris Bioscience, Bristol, UK) was dissolved to 20 mM in DMSO and stored at +4 °C. cAMPS-Rp (Tocris Bioscience) was used as a competitive antagonist of cAMP-induced Protein Kinase A (PKA). Stock solutions of cAMPS-Rp were prepared to 10 mM in H2O and stored at −20 °C. cAMPS-Rp was injected into Xenopus oocytes during TEVC experiments. Therefore, stock solutions of cAMPS-Rp were diluted 1:1 with an intracellular-analogous solution (IAS) which contained 20 mM NaCl, 130 mM KCl, 2 mM MgCl2 and 5 mM HEPES at pH 7.3. This mixture (9.2 nl) was injected into oocytes, leading to concentrations of ~87 µM cAMPS-Rp per oocyte. Corresponding control experiments were performed with IAS. The membrane permeable cAMP-analogue 8-Br-cAMP (Tocris Bioscience) was prepared as a 10 mM stock solution in H2O and stored at −20 °C.
Drugs were generally applied using a gravity-driven perfusion system. In order to reduce the amount of drugs needed, the membrane-permeable cAMP-analogue 8-Br-cAMP was washed into the perfusion chamber and the perfusion was stopped afterwards. The PKA inhibitor cAMPS-Rp was directly injected into the oocytes since this strategy was established earlier and achieved adequate inhibition of PKA55.
For electrophysiological transmembrane recordings, outward-anion currents are defined as negative current signals and depicted in all figures as downward-deflections of the current traces. Data are presented as individual data points (grey symbols) as well as means ± standard error of the mean (SEM). The number of individual oocytes is indicated with ‘n’, whereas the number of donor frogs is represented by ‘N’. Statistical analysis of data was performed with GraphPad Prism version 5 (La Jolla, California, USA). Data were analysed for normal distribution by the Kolmogorov-Smirnov test. For paired experiments, Student’s paired t-test or non-parametric Wilcoxon matched-pairs test (two-tailed) were used. Independent experiments were compared with Student’s unpaired t-test or non-parametric Mann-Whitney test (two-tailed). Mutiple comparison analysis was performed by Kruskall-Wallis test followed by Dunn’s Multiple Comparison Test. P-values ≤ 0.05 were regarded as statistically significant and marked with an asterisk (*). P-values ≤ 0.01 and ≤0.001 were marked with “**” and “***”, respectively.
Ratjen, F. et al. Cystic fibrosis. Nature reviews. Disease primers 1, 15010, doi:10.1038/nrdp.2015.10 (2015).
Farinha, C. M., Swiatecka-Urban, A., Brautigan, D. L. & Jordan, P. Regulatory Crosstalk by Protein Kinases on CFTR Trafficking and Activity. Frontiers in chemistry 4, 1, doi:10.3389/fchem.2016.00001 (2016).
Berger, A. L., Ikuma, M. & Welsh, M. J. Normal gating of CFTR requires ATP binding to both nucleotide-binding domains and hydrolysis at the second nucleotide-binding domain. Proceedings of the National Academy of Sciences of the United States of America 102, 455–460, doi:10.1073/pnas.0408575102 (2005).
Sheppard, D. N. & Welsh, M. J. Structure and function of the CFTR chloride channel. Physiological Reviews 79, S23–45 (1999).
Boucher, R. C. New concepts of the pathogenesis of cystic fibrosis lung disease. The European respiratory journal: official journal of the European Society for Clinical Respiratory Physiology 23, 146–158 (2004).
Widdicombe, J. H. & Wine, J. J. Airway Gland Structure and Function. Physiological Reviews 95, 1241–1319, doi:10.1152/physrev.00039.2014 (2015).
Shamsuddin, A. K. M. & Quinton, P. M. Surface Fluid Absorption and Secretion in Small Airways. The Journal of physiology, doi:10.1113/jphysiol.2012.230714 (2012).
Kunzelmann, K. & Mehta, A. CFTR: a hub for kinases and crosstalk of cAMP and Ca2+. The FEBS journal 280, 4417–4429, doi:10.1111/febs.12457 (2013).
Wang, R. Physiological implications of hydrogen sulfide: a whiff exploration that blossomed. Physiological reviews 92, 791–896, doi:10.1152/physrev.00017.2011 (2012).
Wallace, J. L. & Wang, R. Hydrogen sulfide-based therapeutics: exploiting a unique but ubiquitous gasotransmitter. Nature Reviews Drug Discovery 14, 329–345, doi:10.1038/nrd4433 (2015).
Kimura, H. Metabolic turnover of hydrogen sulfide. Frontiers in physiology 3, 101, doi:10.3389/fphys.2012.00101 (2012).
Bailey, T. S., Zakharov, L. N. & Pluth, M. D. Understanding Hydrogen Sulfide Storage: Probing Conditions for Sulfide Release from Hydrodisulfides. Journal of the American Chemical Society 136, 10573–10576, doi:10.1021/ja505371z (2014).
Kimura, H. Hydrogen sulfide and polysulfides as biological mediators. Molecules (Basel, Switzerland) 19, 16146–16157, doi:10.3390/molecules191016146 (2014).
Olson, K. R. Hydrogen sulfide as an oxygen sensor. Clinical chemistry and laboratory medicine: CCLM / FESCC 51, 623–632, doi:10.1515/cclm-2012-0551 (2013).
Pouokam, E. & Althaus, M. Epithelial Electrolyte Transport Physiology and the Gasotransmitter Hydrogen Sulfide. Oxidative Medicine and Cellular Longevity 2016, 4723416, doi:10.1155/2016/4723416 (2016).
Macfarlane, G. T., Gibson, G. R. & Cummings, J. H. Comparison of fermentation reactions in different regions of the human colon. Journal of Applied Bacteriology 72, 57–64, doi:10.1111/j.1365-2672.1992.tb04882.x (1992).
Blachier, F. et al. Luminal sulfide and large intestine mucosa: friend or foe? Amino Acids 39, 335–347, doi:10.1007/s00726-009-0445-2 (2010).
Hennig, B. & Diener, M. Actions of hydrogen sulphide on ion transport across rat distal colon. British journal of pharmacology 158, 1263–1275, doi:10.1111/j.1476-5381.2009.00385.x (2009).
Pouokam, E. & Diener, M. Mechanisms of actions of hydrogen sulphide on rat distal colonic epithelium. British journal of pharmacology 162, 392–404, doi:10.1111/j.1476-5381.2010.01026.x (2011).
Schicho, R. et al. Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon. Gastroenterology 131, 1542–1552, doi:10.1053/j.gastro.2006.08.035 (2006).
Sun, Q. et al. Hydrogen Sulfide Facilitates Vaginal Lubrication by Activation of Epithelial ATP-Sensitive K(+) Channels and Cystic Fibrosis Transmembrane Conductance Regulator. The journal of sexual medicine 13, 798–807, doi:10.1016/j.jsxm.2016.03.001 (2016).
Lee, Z. W. et al. The slow-releasing hydrogen sulfide donor, GYY4137, exhibits novel anti-cancer effects in vitro and in vivo. PLoS ONE 6, e21077, doi:10.1371/journal.pone.0021077 (2011).
Vitzthum, C., Clauss, W. G. & Fronius, M. Mechanosensitive activation of CFTR by increased cell volume and hydrostatic pressure but not shear stress. Biochimica et biophysica acta 1848, 2942–2951, doi:10.1016/j.bbamem.2015.09.009 (2015).
Ostedgaard, L. S. et al. The DeltaF508 mutation causes CFTR misprocessing and cystic fibrosis-like disease in pigs. Science translational medicine 3, 74ra24, doi:10.1126/scitranslmed.3001868 (2011).
Kimura, Y., Dargusch, R., Schubert, D. & Kimura, H. Hydrogen sulfide protects HT22 neuronal cells from oxidative stress. Antioxid Redox Signal 8, 661–670, doi:10.1089/ars.2006.8.661 (2006).
Moran, O. & Zegarra-Moran, O. On the measurement of the functional properties of the CFTR. Journal of cystic fibrosis: official journal of the European Cystic Fibrosis Society 7, 483–494, doi:10.1016/j.jcf.2008.05.003 (2008).
Kimura, H. Hydrogen sulfide induces cyclic AMP and modulates the NMDA receptor. Biochemical and biophysical research communications 267, 129–133, doi:10.1006/bbrc.1999.1915 (2000).
Mathai, J. C. et al. No facilitator required for membrane transport of hydrogen sulfide. Proceedings of the National Academy of Sciences of the United States of America 106, 16633–16638, doi:10.1073/pnas.0902952106 (2009).
Muzaffar, S. et al. H2S-donating sildenafil (ACS6) inhibits superoxide formation and gp91phox expression in arterial endothelial cells: role of protein kinases A and G. British journal of pharmacology 155, 984–994, doi:10.1038/bjp.2008.326 (2008).
Nagpure, B. V. & Bian, J.-S. Hydrogen Sulfide Inhibits A2A Adenosine Receptor Agonist Induced β-Amyloid Production in SH-SY5Y Neuroblastoma Cells via a cAMP Dependent Pathway. PloS one 9, e88508, doi:10.1371/journal.pone.0088508 (2014).
Yang, H.-Y., Wu, Z.-Y., Wood, M., Whiteman, M. & Bian, J.-S. Hydrogen sulfide attenuates opioid dependence by suppression of adenylate cyclase/cAMP pathway. Antioxidants & redox signaling 20, 31–41, doi:10.1089/ars.2012.5119 (2014).
Yan, S. Z., Hahn, D., Huang, Z. H. & Tang, W. J. Two cytoplasmic domains of mammalian adenylyl cyclase form a Gs alpha- and forskolin-activated enzyme in vitro. J Biol Chem 271, 10941–10945 (1996).
Kobayashi, T., Ikeda, K. & Kumanishi, T. Functional characterization of an endogenous Xenopus oocyte adenosine receptor. Br J Pharmacol 135, 313–322, doi:10.1038/sj.bjp.0704475 (2002).
Sullivan, S. K., Agellon, L. B. & Schick, R. Identification and partial characterization of a domain in CFTR that may bind cyclic nucleotides directly. Curr Biol 5, 1159–1167 (1995).
Bucci, M. et al. Hydrogen sulfide is an endogenous inhibitor of phosphodiesterase activity. Arteriosclerosis, thrombosis, and vascular biology 30, 1998–2004, doi:10.1161/ATVBAHA.110.209783 (2010).
Coletta, C. et al. Hydrogen sulfide and nitric oxide are mutually dependent in the regulation of angiogenesis and endothelium-dependent vasorelaxation. Proceedings of the National Academy of Sciences of the United States of America 109, 9161–9166, doi:10.1073/pnas.1202916109 (2012).
Modis, K., Panopoulos, P., Coletta, C., Papapetropoulos, A. & Szabo, C. Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A. Biochemical Pharmacology 86, 1311–1319, doi:10.1016/j.bcp.2013.08.064 (2013).
Filipovic, M. R. Vol. 230 29–59.
Duckworth, B. C., Weaver, J. S. & Ruderman, J. V. G2 arrest in Xenopus oocytes depends on phosphorylation of cdc25 by protein kinase A. Proceedings of the National Academy of Sciences of the United States of America 99, 16794–16799, doi:10.1073/pnas.222661299 (2002).
Voronina, E. & Wessel, G. M. The regulation of oocyte maturation. Current topics in developmental biology 58, 53–110 (2003).
Nader, N., Courjaret, R., Dib, M., Kulkarni, R. P. & Machaca, K. Release from Xenopus oocyte prophase I meiotic arrest is independent of a decrease in cAMP levels or PKA activity. Development 143, 1926–1936, doi:10.1242/dev.136168 (2016).
Sadler, S. E. & Maller, J. L. In vivo regulation of cyclic AMP phosphodiesterase in Xenopus oocytes. Stimulation by insulin and insulin-like growth factor 1. The Journal of biological chemistry 262, 10644–10650 (1987).
Yong, Q. C., Pan, T. T., Hu, L. F. & Bian, J. S. Negative regulation of beta-adrenergic function by hydrogen sulphide in the rat hearts. J Mol Cell Cardiol 44, 701–710, doi:10.1016/j.yjmcc.2008.01.007 (2008).
Lu, M., Liu, Y. H., Ho, C. Y., Tiong, C. X. & Bian, J. S. Hydrogen sulfide regulates cAMP homeostasis and renin degranulation in As4.1 and rat renin-rich kidney cells. Am J Physiol Cell Physiol 302, C59–66, doi:10.1152/ajpcell.00341.2010 (2012).
Lim, J. J., Liu, Y. H., Khin, E. S. & Bian, J. S. Vasoconstrictive effect of hydrogen sulfide involves downregulation of cAMP in vascular smooth muscle cells. Am J Physiol Cell Physiol 295, C1261–1270, doi:10.1152/ajpcell.00195.2008 (2008).
Kokkonen, K. & Kass, D. A. Nanodomain Regulation of Cardiac Cyclic Nucleotide Signaling by Phosphodiesterases. Annu Rev Pharmacol Toxicol 57, 455–479, doi:10.1146/annurev-pharmtox-010716-104756 (2017).
Pouokam, E., Steidle, J. & Diener, M. Regulation of colonic ion transport by gasotransmitters. Biological & pharmaceutical bulletin 34, 789–793 (2011).
Althaus, M., Urness, K., Clauss, W., Baines, D. & Fronius, M. The gasotransmitter hydrogen sulphide decreases Na(+) transport across pulmonary epithelial cells. British journal of pharmacology 166, 1946–1963, doi:10.1111/j.1476-5381.2012.01909.x (2012).
Erb, A. & Althaus, M. Actions of hydrogen sulfide on sodium transport processes across native distal lung epithelia (Xenopus laevis). PloS one 9, e100971, doi:10.1371/journal.pone.0100971 (2014).
Agné, A. M. et al. Hydrogen sulfide decreases β-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 308, R636–R649, doi:10.1152/ajpregu.00489.2014 (2015).
Walters, D. V., Ramsden, C. A. & Olver, R. E. Dibutyryl cAMP induces a gestation-dependent absorption of fetal lung liquid. Journal of applied physiology (Bethesda, Md.: 1985) 68, 2054–2059 (1990).
Baines, D. Kinases as targets for ENaC regulation. Current molecular pharmacology 6, 50–64 (2013).
Perniss, A., Wolf, A., Wichmann, L., Schonberger, M. & Althaus, M. Evans Blue is not a suitable inhibitor of the epithelial sodium channel delta-subunit. Biochemical and biophysical research communications 466, 468–474, doi:10.1016/j.bbrc.2015.09.052 (2015).
DeLeon, E. R., Stoy, G. F. & Olson, K. R. Passive loss of hydrogen sulfide in biological experiments. Analytical biochemistry 421, 203–207, doi:10.1016/j.ab.2011.10.016 (2012).
Weber, W. M., Cuppens, H., Cassiman, J. J., Clauss, W. & Van Driessche, W. Capacitance measurements reveal different pathways for the activation of CFTR. Pflugers Arch 438, 561–569 (1999).
The authors thank Liesa Kristin Beuter as well as Raphael J. Courjaret and Khaled Machaca (Weill Cornell Medicine Qatar) for help with some experiments and Mirjam Buß and Anja Schnecko for excellent technical assistance. Nikolaus P. Dittrich, Lukas Wichmann and Pawel P. Szczesniak are acknowledged for helpful comments on the manuscript. This work is supported by a grant from the German Research Foundation (DFG; AL1453/1-2) to MA.
The authors declare that they have no competing interests.
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A correction to this article is available online at https://doi.org/10.1038/s41598-018-21979-6.
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Perniss, A., Preiss, K., Nier, M. et al. Hydrogen sulfide stimulates CFTR in Xenopus oocytes by activation of the cAMP/PKA signalling axis. Sci Rep 7, 3517 (2017). https://doi.org/10.1038/s41598-017-03742-5
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