Abstract
We introduce an in silico model for the initial spread of an aberrant phenotype with Warburg-like overflow metabolism within a healthy homeostatic tissue in contact with a nutrient reservoir (the blood), aimed at characterizing the role of the microenvironment for aberrant growth. Accounting for cellular metabolic activity, competition for nutrients, spatial diffusion and their feedbacks on aberrant replication and death rates, we obtain a phase portrait where distinct asymptotic whole-tissue states are found upon varying the tissue-blood turnover rate and the level of blood-borne primary nutrient. Over a broad range of parameters, the spreading dynamics is bistable as random fluctuations can impact the final state of the tissue. Such a behaviour turns out to be linked to the re-cycling of overflow products by non-aberrant cells. Quantitative insight on the overall emerging picture is provided by a spatially homogeneous version of the model.
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Introduction
Overflow metabolism, namely the incomplete oxidation of nutrients at high intake rates leading to the secretion of partially metabolized products, is ubiquitous in cellular systems. Fast growth or high rates of energy expenditure are accompanied by the excretion of carbon equivalents like acetate in E. coli 1, ethanol in yeast2, lactic acid in skeletal muscle cells3, and lactate in many types of tumors4 (where this is known as the Warburg effect). A similar scenario has been observed in immune cells5. Many studies have addressed the functional role6 as well as the origin of this kind of metabolic re-programming at different levels, focusing most recently on the constraints on cellular energy metabolism imposed by different metabolic demands7,8,9, macromolecular crowding10, costs associated to gene expression11,12,13, membrane occupancy14 or intrinsic limits to mitochondrial oxidative phosphorylation15, as well as on hypoxia16 and dynamical mechanisms like flux sensors17 (see refs 18, 19 for recent reviews of different models and possible mechanisms).
The effects of overflow in cell populations, however, specifically for the creation of functionally relevant microenvironments, are less studied. While it has been argued that the competition between slow-growing oxidizers and fast-growing fermenters may have played a central role in the evolution of multicellularity20, 21, empirical evidence suggests that an effective cooperation between these distinct phenotypes may arise from the competition for nutrients, with important physiological consequences. The simplest manifestation of such a regime is via the shuttling of overflow products22. In short, fast growing cells typically outcompete their quiescent neighbours for the primary carbon source. In such conditions, the latter may have to re-adjust their metabolism, ultimately relying on the carbon equivalents excreted by the former for survival. This kind of cell-to-cell coupling has been found to play an important role in multicellular contexts ranging from cancer23 to muscle cells24 to brain energy metabolism25, 26. In cancer, the lactate-shuttle scenario has been analyzed theoretically27 and in silico 28, experimentally through indirect genetic evidence23, and has also been discussed as a potential therapeutic target29 in the recent revival of interest in the re-programming of metabolism in cancer30 (but see also ref. 31). Taken together with the growing literature on tumor-stroma interactions32, 33, these results present cancer as a strongly non-cell-autonomous disease and suggest a major functional role for the cooperative coupling between overflowing and non-overflowing cells. Similar scenarios have been studied in the context of microbial populations34,35,36.
Besides strictly energetic considerations related to the usefulness of overflow products (like lactate, acetate, ethanol and ammonia) as metabolic fuels, their shuttling might serve the added purpose of clearing the extracellular space of the potentially toxic side-effects caused by their accumulation. The toxicity of lactate and ammonia has been well characterized for mammalian cell cultures37, 38 and cancer39, 40, in which case the increased microenvironment acidity caused by extracellular lactate has been found to stimulate the death of surrounding cells41, 42. In turn, acetate can inhibit E. coli growth by as much as 50%1, 43 while yeast’s tolerance to ethanol is known to be strongly strain-dependent44. Likewise, industrial mammalian cell cultures are commonly affected by the accumulation of toxic metabolites secreted during the production process and exhibit qualitatively distinct metabolic states in response to variations in extracellular metabolite concentrations, transitioning from efficient, oxidative phenotypes to inefficient wasteful states45. In this context the accumulation of waste is usually undesired and one strives to maintain cells that can fully metabolize their nutrients into compounds of interest, posing an important biotechnological challenge46.
Here we attempt to study in silico how carbon overflow affects the overall metabolic organization of cells in a tissue, including spatial effects as well as effects due to shuttling, microenvironment toxification and exchanges with the blood. Our scenario is close to that considered in models of avascular tumor growth47,48,49 but integrates a coarse-grained perspective of cell metabolism along the lines of refs 28, 50 and the acidification of the environment due to the byproducts of metabolism. In particular, we assume that, in a tissue formed by healthy cells which can exchange compounds with a chemical reservoir, a single aberrant cell is planted, with upregulated primary nutrient transporters and disregulated energetics leading to carbon overflow and (stochastically) aberrant cell death and/or replication. We analyze the conditions under which the competition for the primary carbon source can be turned into a cooperative regime where non-aberrant cells are sustained by the aberrant ones’ overflow product, in turn fostering the spread of the latter. By extensive numerical simulations, we obtain a “phase structure” in which the outcome is studied as a function of the concentration c of primary nutrient in the reservoir and of the characteristic turn-over rate k for tissue-reservoir exchanges. We furthermore develop a mathematically solvable version of the model where spatial effects are neglected, allowing to unveil the physical origin of many of the key features found in its spatial counterpart. Our results suggest that the accumulation of waste can limit the growth of the aberrant cells, leading to their demise, unless remediated either by re-cycling via healthy cells or by export away from the tissue. In such a scenario, c and k are the key variables controlling whether the aberrant cells will spread to the entire tissue. However, in the spatial model, random fluctuations can drive the tissue toward different outcomes. This bistability is absent in the spatially homogeneous version and is tightly coupled to the shuttling of overflow products.
Results
Model definition and properties
Basic setup
We consider an ensemble of cells (a “tissue” for short) in contact with a nutrient reservoir (the “blood”, see Fig. 1A). The tissue is modeled as a single layer of cells arranged in an N × N square lattice, with each site i occupied by a cell. A primary carbon source S (say, glucose) is available in the blood at a fixed concentration denoted by c, and can diffuse through the blood-tissue barrier and across the tissue. Likewise, a waste product of the metabolism of cells within the tissue can diffuse across the tissue or be exported into the blood. The effect of metabolite exchange with the blood is quantified by an effective metabolite turn-over time τ.
Cellular metabolic networks
Cells can be of two types, which we shall call “healthy” and “aberrant” respectively. The two types differ slightly in their metabolic capabilities. Specifically, each cell i can import and process S at a rate u i that depends on the local substrate level s i and on its energy demand (see Fig. 1B). The in-taken nutrient is processed into a partially metabolized precursor P and energy E (i.e. ATP), with a theoretical energy yield equal to Y u (i.e. at most Y u units of energy are obtained per unit of u i ). However, while aberrant cells can turn P into a ‘waste’ metabolite W and excrete it, healthy cells can import W and re-cycle it to produce P. The corresponding fluxes v i (v i ≥ 0 for importing healthy cells, v i ≤ 0 for exporting aberrant cells) are determined by the local level of W in the tissue (denoted by w i ) and by cellular energy demands. Finally, energy can be derived from P at rate r i given, by mass-balance, by r i = u i + v i ≥ 0. Assuming a yield for the P → E conversion equal to Y r , the energy output flux obtainable from S and W is
Identifying r i with the flux through respiration, we assume it is capped50 so that 0 ≤ r i ≤ r max. We furthermore assume that Y u < Y r , by analogy with the universally lower yield of fermentation versus respiration.
Metabolic objectives and optimal flux patterns
Aberrant and healthy cells also differ strongly both in their maximum S-intake capability, with aberrant cells assumed to be able to sustain much higher values of u i than healthy cells, and in their respective metabolic goals, in that the former are assumed to maximize their energy output (1) while the latter only aim at generating a fixed maintenance energy output flux e m . In addition, we conform to empirical in vitro studies showing that cells may consume a waste product of their metabolism like lactate or acetate only after glucose is depleted46, 51, and assume that cells employ the primary nutrient preferentially. These assumptions lead to specific forms for the key fluxes u i and v i . Defining \({u}_{{\rm{MM}}}({s}_{i})={u}_{{\rm{\max }}}{s}_{i}/({K}_{{\rm{s}}}+{s}_{i})\) and \({v}_{{\rm{MM}}}({w}_{i})={v}_{{\rm{\max }}}{w}_{i}/({K}_{{\rm{w}}}+{w}_{i})\) as the fluxes of standard Michaelis-Menten uptake kinetics with parameters (u max, K s) and (v max, K w), and denoting by \({u}_{m}\equiv {e}_{m}/({Y}_{u}+{Y}_{r})\) the smallest value of u i ensuring that e i = e m without resorting to W (i.e. for v i = 0) one has that, for healthy cells,
whereas for aberrant cells
with \({\alpha }_{{\rm{ab}}}\gg 1\) a constant quantifying the dysregulated uptake capacity of aberrant cells. In words, for healthy cells, if u MM(s i ) > u m then u i = u m and no in-take of W is required to ensure that e i = e m (i.e. v i = 0). On the other hand, for u MM(s i ) < u m cells will import as much of S as possible and then import either the minimal amount of W required to meet their energy demand \(({v}_{i}=({u}_{m}-{u}_{i})(1+\tfrac{{Y}_{u}}{{Y}_{r}}))\) or as much W as possible according to local availability (v i = v MM(w i )). For aberrant cells, instead, if u i < r max then all the primary nutrient imported can be processed through r i and no precursor is excreted (v i = 0). When u i exceeds r max, though, W is produced and secreted at net rate |v i | = u i − r max.
Aberrant cells death and replication dynamics
A feedback between the population dynamics of aberrant cells and waste accumulation in the tissue is established by the fact that both their replication (λ i ) and death (σ i ) rates depend on the local level of waste w i (see Fig. 1C). In particular, we assume that every aberrant cell with at least one healthy neighbor can replicate, with the daughter cell occupying the site of a randomly picked normal neighbor, with a probability per unit time given by
where λ max represents the fastest growth rate achievable by aberrant cells while K inh and K E are constants. In short, the growth rate is zero unless cells exceed the maintenance energy flux e m , it increases in a Monod fashion as e i grows, but gets smaller as w i increases52. The particular choice for the dependence on w i avoids the introduction of extra parameters (compared for instance to a Hill function) while returning the same qualitative behaviour. Likewise, we assume the death rate σ i to be given by refs 53, 54
which becomes larger (and tends to the limiting value σ max) as w i increases. We assume that aberrant cells are replaced by healthy ones upon dying. Note that we don’t consider a specific birth/death dynamics for healthy cells, implicitly assuming that they are found in all tissue sites that are not occupied by aberrant cells (e.g. both aberrant and healthy cells are immediately replaced by healthy ones at death). In such conditions, the growth dynamics of healthy cells is immaterial.
Diffusion of primary nutrient and waste
Finally, the local concentrations of the primary nutrient (s i ) and of the precursor (w i ) are assumed to change in time and space according to
where \(k\equiv 1/\tau \). The first terms on the right-hand-sides of (8, 9) represent the local concentration smoothing due to diffusion, with D S and D W the diffusion coefficients, d the number of neighbors of cell i (d = 4 in our case) and the sum runs over the set \({\mathscr{N}}(i)\) of neighbors of cell i. The second terms describe the changes due to import or export of compounds by cells, with u i and v i depending on concentrations as discussed in (2), (3), (4) and (5). The third terms denote instead the variations due to the exchange of primary nutrient and, respectively, the removal of the precursor from the tissue, in which the blood acts as an external reservoir for S and as a sink for W.
Time evolution
After initiating the system in a configuration with a single aberrant cell among healthy cells (time t = 0), the dynamics of aberrance spread depends on (i) the pattern of local availability of primary nutrient, and (ii) how efficiently growth-suppressing waste can be removed from the tissue, either via the blood or via recycling by healthy cells. Overall, this scenario describes the conditions for the emergence of a mutually beneficial (local) microenvironment, characterized by a W-mediated metabolic coupling between healthy and aberrant cells. We are interested in quantifying its role in the sustainment of aberrant growth and the survival of healthy cells, and in understanding how it relates to the macroscopic environmental parameters c (the concentration of primary nutrient in the blood) and τ = k −1 (metabolite turn-over time).
Dynamics of aberrant spread
We carried out extensive numerical simulations of the model upon changing the key environmental parameters c and k, after having rendered parameters non-dimensional and having set them at realistic representative values (see Methods). Typical snapshots from a full-scale simulation of a 20 × 20 tissue obtained for k = 103 and c = 0.1 are shown in Fig. 2A. Initially, the aberrance spreads and waste is secreted (red markers, v < 0), while nearby healthy cells are deprived of primary nutrient and start to rely on the waste secreted by aberrant cells for survival (pink markers, v > 0). Growth of the aberrant population is therefore sustained by the coupling with healthy cells. Eventually, though, the primary nutrient gets depleted and aberrant cells in the core no longer saturate the respiration flux (black markers, v = 0), thereby ceasing waste secretion.
Figure 2B,C summarize the main features of the time evolution of the system observed in the simulation. The mean local concentration of primary nutrient s decreases with time while the average waste level w increases rapidly until it peaks (black arrow). At that point, healthy cells begin to in-take and recycle the waste product of aberrant growth for survival when their maintenance demands cannot be met from the primary nutrient alone (see Fig. 2C). A noticeable acceleration in aberrant growth then occurs (viz. the change in slope at the black dashed line in Fig. 2B) as the waste concentration begins to decrease and the environment becomes less toxic. As s decreases, W becomes the main metabolic fuel of healthy cells, while a fraction of aberrant cells in the core stops secreting W, further pushing down the level of waste. At the peak of waste uptake by healthy cells (green arrow), a second inflection point in aberrant growth can be observed (viz. the slope change at the green dashed line in Fig. 2B). The mean waste level vanishes shortly thereafter. Eventually the entire tissue is taken over by aberrant cells and ϕ saturates at one.
Phase portrait
Results concerning dynamical features and final configurations can be summarized in the ‘phase portrait’ displayed in Fig. 3A in terms of the asymptotic value of the mean fraction ϕ of aberrant cells in the tissue as a function of c and of the effective blood-tissue turn-over rate k (averages over 20 runs). At low nutrient level and long turnover times (region labeled W−), the primary source cannot support aberrant growth and the tissue ultimately stays healthy. In this region, no waste accumulates in the tissue and therefore no waste recycling occurs. Upon increasing k and/or c (region labeled Sh.), one enters a phase in which metabolic coupling with waste recycling occurs during the dynamics. Interestingly, though, if k gets sufficiently large, the aberrance can spread to the whole tissue, strongly implicating the metabolic coupling and/or enhanced remediation through the bloodstream as keys for tissue takeover by aberrant cells. A third regime (region labeled W+) occurs if k or c are sufficiently large, in which healthy cells can meet their energy demand thanks to the large availability of primary substrate, so that no recycling takes place. If the increase in c is not coupled to an increase in k, waste accumulates in the tissue because blood exchange alone does not suffice to remove W (k too low), thereby repressing aberrant growth due to increasing toxicity. As a consequence, the tissue stays healthy in the long run. On the other hand, if k also increases, aberrant cells ultimately take over the tissue as the growth-suppressing waste product is efficiently cleared from the tissue thanks to the high turn-over rate.
Insights from a solvable mathematical model
Spatially homogeneous model
The origin of the rich behavior observed in silico can be mathematically traced by a simplified, analytically tractable version of the model. The simplification consists in assuming spatial homogeneity, i.e. that the transport and diffusion of the primary substrate S and of the waste product W are sufficiently fast for all cells to sense the same local levels of both species. This leads to a “mean-field” type of model that ignores the spatial organization of cells. The state of the tissue can be fully described in terms of three variables, namely the fraction of aberrant cells ϕ, the level of primary nutrient in tissue s and the level of waste product in tissue w. Assuming that the daughters of aberrant cells replace normal cells in a population constrained to a fixed total size, the above quantities evolve in time according to
where the parameters λ (replication rate of aberrant cells), σ (death rate of aberrant cells), u he and u ab (primary nutrient intake flux of a healthy and, respectively, aberrant cell), and v he and v ab (the waste intake and, respectively, out-take flux of a healthy and, respectively, aberrant cell) depend in turn on s and w as in Equations (2)–(7). Equation (10) describes the change in ϕ due to duplication and death events (both of which require the vicinity of a healthy cell) at fixed total number of cells. The evolution of s and w, instead, is due to cellular import and export activities and to exchanges with the blood.
Coupling fast homogenization with a quasi-steady-state assumption for concentration variables (based on the fact that the time scales characterizing cell replication are much longer than those over which tissue levels of primary nutrient and waste equilibrate) allows to decouple the dynamics of s and w from that of ϕ. Specifically, setting \(\frac{ds}{dt}=\frac{dw}{dt}=0\), one may express both quantities as functions of ϕ, thereby leaving the latter as the key variable of the model. Numerical solution of its equilibrium value from Eq. (10) (see Methods for the choice of parameters) finally results in the phase portrait displayed in Fig. 4A. The homogeneous model appears to match closely its spatial counterpart (Fig. 3) except for a key detail, provided in Fig. 4B. In specific, one sees that the spatial model displays coexistence of fully aberrant states with fully healthy states for low k and intermediate c (region Sh.), i.e. for the same values of c and k different runs may end up with the tissue being taken over by aberrant cells or with a completely healthy tissue, while the simplified model is unable to capture this aspect.
Cutting through the (k, c) diagram at different levels of c (displayed as yellow lines in Fig. 4A) different scenarios are highlighted (see Fig. 4C). For low c, ϕ gradually increases with k until it saturates close to the maximum. Upon further increasing k, one generates a waste shuttling from aberrant to healthy cells with accumulation of waste in the tissue, which is remediated at higher k by faster wash-away into the blood stream. At high c, instead, aberrant cells can survive even at low k without secreting waste. If k increases, though, waste excretion sets in that is not managed since (i) the local level of primary nutrient is large enough for healthy cells to survive based on it alone, and (ii) removal by the blood is still too slow. As a consequence, aberrant growth is repressed and ϕ decreases. A further increase of k however allows to re-start aberrant growth by clearing up the micro-environment.
The state of the tissue is regulated by a series of thresholds
It is remarkable that the transition between full aberrant dominance and healthy tissue in some cases turns out to be discontinuous, viz. the separation between the Sh. and W+ regions marked by a blue line in Fig. 4A and by a blue arrow in Fig. 4C. In such conditions, small variations in k or c suffice to cause large-scale changes in the final tissue state. The same behaviour is observed in the fully spatial model (cf. Fig. 4B). This highlights the existence of thresholds where the qualitative characteristics of aberrant growth and tissue metabolism change drastically. Within the solvable model, it is possible to fully trace their origin to the parameters of the model. Mathematical analysis (see Supporting Text) leads in particular to the identification of three thresholds for the tissue level of primary substrate, i.e.
or, equivalently, in terms of the fraction of aberrant cells (see Fig. 5A),
using which the phase structure can be understood. Note that we assume u m ≤ r max (see Methods), so that s gr ≤ s sec ≤ s m .
Starting from (13), healthy cells are found to be able to sustain their maintenance demands from the primary nutrient alone if s ≥ s m , while for s < s m they must rely on re-cycling of W (if available). Aberrant cells, on the other hand, can have a non-zero replication rate (Eq. 6) if s > s gr and they secrete W if s > s sec. Therefore, a metabolic coupling with shuttling of W from aberrant to healthy cells may only exist in the range s sec < s < s m . (In turn, s m > s sec or, equivalently, r max < α ab u m is a necessary condition for metabolic coupling).
In terms of the fraction of aberrant cells, instead, the thresholds (14) simply represent the values of ϕ that are consistent with the corresponding quasi-steady state nutrient level. So, for instance, the growth rate of aberrant cells is zero when ϕ ≥ ϕ gr, implying that ϕ gr is an upper bound for the fraction of aberrant cells in the system. On the other hand ϕ m is the maximum fraction of aberrant cells consistent with healthy cells satisfying their maintenance demands from the consumption of primary nutrient alone, while waste secretion occurs if ϕ < ϕ sec. Therefore, a waste shuttle takes place when ϕ m < ϕ < ϕ sec. The thresholds (14) are shown in Fig. 5B as functions of k and c.
Physical origin of the different phases
Comparing the asymptotic aberrant fraction ϕ f to the thresholds Eq. (14) yields three possible outcomes (namely ϕ f ≤ ϕ m , ϕ m < ϕ f < ϕ sec and ϕ f ≥ ϕ sec). The corresponding regions in the (k, c) plane are shown in Fig. 5C. If ϕ f ≤ ϕ m (Region I in Fig. 5C), healthy cells satisfied their maintenance demand from S alone throughout the dynamics, while aberrant cells exhibited sustained waste secretion. In this region, aberrant growth is limited by excess toxicity and aberrant take-over is only achieved if the turn-over rate k is large enough to ensure remediation. A different scenario is obtained when ϕ m < ϕ f < ϕ sec (Region II in Fig. 5C). Here, although the waste secreted by aberrant cells was initially uncoupled to the metabolism of healthy cells, eventually ϕ crosses the threshold ϕ m , a waste shuttle kicks-in and is sustained from then on. Again, the growth of aberrant cells is affected by waste toxicity, but its effects are now countered by a combination of healthy cell recycling and the turn-over rate k. Finally, if ϕ f ≥ ϕ sec, waste secretion and recycling are not sustained because at some point in the dynamics ϕ becomes larger than the threshold ϕ sec. This leads to a state where aberrant growth can only be limited by nutrient availability. In this scenario, either the primary nutrient does not suffice to sustain a fully aberrant state (ϕ f = ϕ gr in this case), or the aberrance eventually takes over the tissue (ϕ f = 1). In other words, ϕ f = min{1, ϕ gr}. Additionally, ϕ sec may become negative in Region III, defining a sub-region where waste is never secreted (Region IIIb in Fig. 5C). On the other hand, the portion of region III where ϕ sec > 0 (Region IIIa in Fig. 5C) has a transient waste secretion.
In Fig. 4A the (k, c) plane was split according to the role of waste. Confronting the two classifications one sees that region W+ coincides with region I as defined above, while region Sh. turns out to be composed of Regions II (where shuttling, once started, is sustained indefinitely) and IIIa (where waste secretion and shuttling are transient). Finally, region W− coincides with IIIb. The disjoint white areas of Fig. 4A where aberrant cells fail to expand to the entire tissue, one lying inside W− and the other inside W+, are now explicitly seen to trace back to different causes, namely nutrient scarcity for W− and waste toxicity for W+. Additionally, the sharp discontinuity highlighted by the blue boundary in Fig. 4A finds a simple interpretation. On the right side of the edge, aberrant growth is limited by toxicity and ϕ f < ϕ sec. On the left side, instead, waste is transient and nutrient availability becomes the determining factor, i.e. ϕ f = min{1, ϕ gr}. The discontinuity therefore simply describes the transition from a state with ϕ f < ϕ sec to one with ϕ f = min{1, ϕ gr}.
The role of random fluctuations in the dynamics of the spatial model
As pointed out above, while the phase diagrams in Figs 3 and 4 are qualitatively very similar, a major difference derives from fluctuations in the spatial model. In particular, in the parameter region where waste shuttling occurs, the spatial model exhibits a bistable behaviour such that random fluctuations may drive the tissue towards a fully aberrant or a fully healthy state (see Fig. 4A,B). Sample trajectories displaying this phenomenon are shown in Fig. 6. For the same initial conditions and parameters, we plot the fraction ϕ of aberrant cells versus time in a simulation of the mean-field model (blue line) and in several simulations of the spatial model (red and black lines). Trajectories where the spatial model’s outcome qualitatively agrees with the mean-field model are shown in black, while cases in which the spatial model diverges from the mean-field behaviour are shown in red. One sees that fluctuations can bring the tissue to a healthy state when most trajectories converge to an aberrant one (as in Fig. 6A) or vice versa (as in Fig. 6B).
Intuition about how fluctuations may lead to different outcomes can be obtained by noting that, in terms of the thresholds derived for the mean-field model, toxicity limits aberrant growth only if ϕ f < ϕ sec whereas waste accumulation ceases if the aberrant fraction reaches a value larger than ϕ sec. In between, a critical value of ϕ exists beyond which waste accumulation decreases if the fraction of aberrant cells increases and aberrant growth becomes positive. If, by random fluctuations, the aberrant cell population overcomes this threshold, it will then continue to grow up to the maximum value allowed by nutrient availability (i.e. min{1, ϕ gr}). In such a case, the mean-field model fails to predict aberrant dominance. Likewise, if random evolution keeps ϕ below ϕ sec long enough, toxicity caused by waste accumulation may lead to the disappearance of aberrant cells from the tissue. This clarifies that the equivalence between the mean-field and spatial models is not full. Remarkably, though, the mean qualitative behavior of the spatial model is well captured by its mean-field approximation.
Since the bistability is localized in a parameter region where waste recycling is prominent, we probed the link between the two occurrences. Specifically, we ran simulations of both models after disabling the shuttle by forcing v he = 0 for healthy cells. A comparison between the original and modified systems is showcased in Fig. 7. We zoom into the parameter region where disabling the shuttle has a stronger impact, corresponding to intermediate to high c and low to intermediate k (see Fig. 3). Random fluctuations resulted in bistability in the spatial model prominently in the region highlighted in blue in Fig. 7A, where aberrant growth is severely inhibited in absence of re-cycling by healthy cells (see Fig. 7B). This strongly suggests that the bistable stochastic dynamics of aberrant spread is enabled by the shuttling of overflow product by aberrant cells to healthy cells for recycling purposes. We then repeated this comparison in the homogeneous model, see Fig. 6C,D. Here silencing the shuttle only appears to bear a quantitative effect. As in Fig. 4A, we find a discontinuous edge (blue line) separating a region where growth is inhibited by toxicity from a region where growth is limited by nutrient availability. In absence of shuttling, the edge simply appears to shift towards slightly lower values of c (see Fig. 7D), indicating that the recycling of toxic overflow products by healthy cells enables aberrant growth for larger supplies of primary nutrient.
Discussion
Almost universally, cells sustaining high energy demands or fast growth undergo an overall shift in metabolic strategy that carries strong signatures in proteome composition and regulatory mechanisms55,56,57. Many explanations have been put forward for the seemingly inefficient use of nutrients that occurs as cells release partially metabolized substances into their surrounding environment58. In some cases, exceeding protein costs prevent the operation of high-yield respiratory at fast growth13, 59, but this scenario does not appear to be generic even for bacteria60. Likewise, crowding or capacity constraints account for the onset of the inefficient phase10 but likely can’t be invoked universally61. The challenge of understanding the fundamental trade-offs driving metabolic strategy selection in different cases is therefore still largely open. In turn, though, metabolic decisions by individual cells can determine significant population-level effects, for instance by inducing cross-feeding interactions62, which then feed back into the fitness of individual cells.
Working along these lines, we have studied here how a spatially distributed population of interacting cells with very different energetic demands self-organizes in a shared environment coupled to a single nutrient reservoir. The model we define can be seen as a minimal approach to the analysis of the initial stages of the spread of an aberrance in a tissue. Many types of tumors generate an acidic microenvironment due to disregulated metabolism and lactate overflow63. Based on empirical evidence, we assume that (i) the tissue exchanges a primary nutrient with a reservoir that also serves as a sink for the overflow product, (ii) lactate shuttling may occur, i.e. non-aberrant neighbours of aberrant cells may intake lactate if the primary nutrient is insufficient to ensure their maintenance, (iii) the replication and death rate of aberrant cells are affected by the local level of lactate, and (iv) both the primary nutrient and lactate can diffuse across the tissue. From a physical viewpoint, the key assumption is that metabolism occurs on faster time scales than across-tissue diffusion, so that cells can effectively optimize their metabolic profile while local concentrations are fixed. Besides modeling details, this is a point where our work departs from the approaches presented e.g. in ref. 36, where a dynamic description of metabolism is employed. Our simplified (in this respect) scenario allows for the study of an extended, spatially organized system with a large number of cells.
Many physiological aspects are however highly schematized. For instance, our representation of the blood as a nutrient reservoir at fixed concentration ignores variability in blood glucose levels, which have been observed to be significant in specific conditions64. An increase of fluctuations is thought to be generically linked to the approach to dynamical transitions65 and would impact the scenario discussed here. Likewise, we have employed a minimal representation of cellular metabolic networks that allows to focus on the organization of tissue-wide energy metabolism while keeping parameters to a controllable minimum. Performing a similar analysis starting from genome-scale models of metabolism would require a significant additional computational effort focused on selecting thermodynamically viable flux patterns66, 67. The qualitative scenario emerging from the present study however is unlikely to be altered in a more complex, parameter-rich setting.
Our results are summed up in the phase portrait of Fig. 3 (similar to the outcome diagram obtained for cross-feeding microbial populations in ref. 36), where the asymptotic state of the tissue (in terms of the fraction ϕ of aberrant cells) is shown as a function of the primary nutrient level s and of the tissue-reservoir turn-over rate k. Three phases appear. In the phase labeled W− (sufficiently small c and k), the spreading dynamics of the Warburg-like phenotype is limited by nutrient availability and no shuttling of overflow product occurs. Upon increasing c and/or k one enters the Sh. region. All over this phase, the aberrant spreading dynamics is sustained by healthy cells re-cycling harmful overflow products. However, the long-term state of the tissue is typically healthy for large c and small k (in which case waste toxicity limits aberrant take-over in spite of microenvironmental cooperation) and aberrant for large k and small c (when a combination of efficient waste re-cycling and fast turn-over with the blood leads to a fully aberrant state). Finally, region W+ is found at large enough c and k. Here, no re-cycling takes place and the long-term state of the tissue is determined essentially by its coupling to the blood (healthy for slow turn-over, aberrant for fast turn-over). We have tracked the physical origin of these phases through a simplified, mathematically tractable version of the model whose solution ultimately relies on just 3 variables (the fraction of aberrant cells and the levels of primary nutrient and waste) and 3 (combinations of) parameters given by the thresholds in (13) or (14). The main feature escaping the solvable model is the presence of bistability, occurring in the spatial model for large c and small k. Here, the final state of the tissue can be strongly influenced by random fluctuations occurring during the dynamics. This phenomenon appears to be coupled to the shuttling of overflow products between aberrant and healthy cells, as revealed by disabling re-cycling by non-aberrant cells in Fig. 7.
Stretching out the information encoded in the phase structure, our simulations suggest different qualitative strategies to slow down aberrant growth. At intermediate k, one may either deprive aberrant cells of primary nutrients by decreasing c or foster growth suppression via tissue toxification by increasing c. For higher k and high enough c, the aberrance spread can be halted by toxicity. In this regime increasing c is the only way to lead the tissue back to a healthy state. Increased glucose supply in tumors is known to lead to faster acidification42, an effect that is usually connected to the production of lactate. Moreover, glucose infusion leads to less efficient blood-tissue turn-over, probably due to enhanced viscosity68. This aspect also contributes to acidification42. Notice that arterial vasodilators may selectively increase perfusion in tissues, leading to a fall in their pH69. Our results also suggest that targeting lactate shuttling in tumors42, 70 may be effective in driving a tissue from an aberrant state towards a healthy state, provided k is sufficiently low and c is high, in agreement with experimental findings29, 71. It is however important to remark that the role of lactate in cancer is turning out to be much broader than the aspects on which we focus here, especially in connection to its interaction with the immune system72. In this sense, our model is more relevant to the understanding of early lesions consisting of a few aberrant cells, which are effectively invisible to the immune system due to their small size.
Methods
Choice of parameters
Our choice of parameters was guided by the example of the glucose-lactate pair (for primary nutrient and overflow product, respectively) in cancer growth.
The ATP yields appearing in (1) were chosen following the simple glycolytic models of refs 28, 50, i.e. Y u = 1 and Y r = 19 (both giving moles of ATP output per mole of S imported). The ATP demand related to maintenance for mammalian cells is usually reported to be around73 e m = 10 pmol/day/h 3, where \(h\simeq 0.01\,{\rm{mm}}\) denotes the typical linear dimension of a cell (see also below). The parameters λ max and K E in (6) were fitted to agree with the approximate linear relation between e − e m and the growth rate at low energy production rates (with a proportionality constant close to 10 pmol/cell)73 and with the observed maximum growth rate of mammalian cells (close to 3 day−1, see ref. 74), obtaining λ max = 2 day−1 and K E = 20 pmol/day/h 3.
The most commonly expressed glucose transporter in mammalian cells is GLUT1, with a reported affinity of 1 mM75, 76. The average physiological concentration of glucose in the blood is around 5 mM. Assuming that the uptake of healthy cells follows a Michaelis-Menten law and that this concentration is enough to sustain the maintenance demand, we can solve for u max in the equation u m = u MM(s) to obtain a value of u max = 0.5 pmol/day/h 3. We re-defined the glucose to lactate stoichiometric ratio to one by doubling glucose levels and setting K s = 2 mM, u max = 1 pmol/day/h 3. To model the up-regulation of glucose transporters in growth-optimizing aberrant cells we use α = 100 in (4). With the example of lactate in mind, with a measured Michaelis constant ranging between 1 mM and 10 mM, we set K w = 5 mM, while the maximum uptake of this metabolite has been reported77,78,79 around v max = 100 pmol/day/h 3.
Measured values of the glucose diffusion coefficient in tissue span values between 10−6 cm2/sec and 10−5 cm2/sec, depending on the tissue and measurement technique80,81,82. We adopted an intermediate common value of the diffusion coefficient for substrate and waste, of 1.5 × 10−6 cm2/sec. Cells in the 2-dimensional grid were assumed to be a distance h apart that reflects the number of cells per unit area in flat tissues. This value varies widely across different tissues83, 84, and we set it to 104 cells/mm2, which implies a typical distance h between cells around 0.01 mm. Coupling this to the coefficients of the discretized Laplacian operator in the 2-dimensional grid (d/h 2), we obtain the value 0.06 sec−1 for the effective diffusion constants D S and D W .
The inhibitory effects of waste on growth are again modeled after lactate. Quantitative expressions have been obtained from in vitro studies. We use the values K inh = 8 mM, K tox = 15 mM and σ max = 2 day−1 from ref. 53, but see also refs 85,86,87.
We assume that cells have a maximum capacity to fully oxidize the precursor P arising e.g. from a limitation in the proteome fraction available for enzyme production12 or by limited mitochondrial efficiency15. Following28, 50, we set r max = 6.48 pmol/day/cell.
Finally, metabolite levels are measured in units of K tox (so that K tox = 1), time is measured in units of 1/σ max, and fluxes are re-scaled by K tox σ max. The detailed definition of non-dimensional parameters is provided in the Supporting Text.
Note that, with these choices, the standard values of c ranging from hypoglycaemic to hyperglycaemic conditions covers about an order of magnitude in the central part of the vertical axis of Figs 3 and 4A. Therefore the overall range of values for c and k over which we characterize both the spatial model and the simplified one is considerably broader. Note however that the physiological range contains each of the different phases and sub-phases that we characterize.
Simulation details
Numerical simulations of the lattice model were carried out in Julia88. The script is available from Github (https://github.com/cossio/aberr). To speed up simulations, we exploited the fact that the spatial distribution of metabolite levels equilibrates fast compared to cell dynamics. Specifically, at each step, we checked whether metabolite levels have equilibrated, and advanced cell dynamics to the next division/death event (following the Gillespie algorithm89) whenever an equilibrium had been achieved. The asymptotic fraction of aberrant cells in the mean field model was computed by simulating the model’s ODEs for a very long time using the ‘NSolve’ routine from Mathematica.
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Acknowledgements
Work supported by the Italian Institute of Technology’s Seed Project DREAM and by the European Union’s Horizon 2020 research and innovation programme MSCA-RISE-2016 under grant agreement No 734439 INFERNET.
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A.D.M. and R.M. conceived research; all authors designed research; J.F.C.D. performed simulations; all authors analyzed results; all authors wrote the manuscript.
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Fernandez-de-Cossio-Diaz, J., De Martino, A. & Mulet, R. Microenvironmental cooperation promotes early spread and bistability of a Warburg-like phenotype. Sci Rep 7, 3103 (2017). https://doi.org/10.1038/s41598-017-03342-3
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DOI: https://doi.org/10.1038/s41598-017-03342-3
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