The antineoplastic drug carmofur is shown to inhibit the SARS-CoV-2 main protease (Mpro). Here, the X-ray crystal structure of Mpro in complex with carmofur reveals that the carbonyl reactive group of carmofur is covalently bound to catalytic Cys145, whereas its fatty acid tail occupies the hydrophobic S2 subsite. Carmofur inhibits viral replication in cells (EC50 = 24.30 μM) and is a promising lead compound to develop new antiviral treatment for COVID-19.
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We are grateful to the staff at beamlines BL17U1, BL18U1 and BL19U1 at Shanghai Synchrotron Radiation Facility (SSRF, China), where data were collected. This work was supported by grants from the National Key R&D Program of China (grant no. 2017YFC0840300 to Z.R. and 2020YFA0707500.), the Project of International Cooperation and Exchanges NSFC (grant no. 81520108019 to Z.R.), the Science and Technology Commission of Shanghai Municipality (grant no. 20431900200), the Department of Science and Technology of Guangxi Zhuang Autonomous Region (grant no. 2020AB40007) and the Natural Science Foundation of China (grant no. 31970165).
The authors declare no competing interests.
Peer review information Inês Chen was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
a, The overall structure of SARS-CoV-2 Mpro in complex with carmofur. The salmon and green represent the different protomers. The carmofur atoms are shown as solid spheres. b, The side view of the complex. c, The first serine participate in the formation of the dimer.
a, Putative inhibition mechanism. Red curve represents Mpro polypeptide and the black sphere represent the Cα of C145. b, Schematic diagram of Mpro-carmofur interactions. Black spheres represent Cα atoms.
a, Overall structural comparison between the Mpro-carmofur and Mpro-N3 complexes. The salmon cartoon represents the carmofur bound structure and the light cyan represents the N3 bound structure. Carmofur, N3 and DMSO are represented by the purple, yellow and green balls and sticks, respectively. b, The binding pocket of Mpro. Carmofur and N3 are represent in the same way as in panel a. c, Schematic diagram of carmofur and N3.
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Jin, Z., Zhao, Y., Sun, Y. et al. Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur. Nat Struct Mol Biol (2020). https://doi.org/10.1038/s41594-020-0440-6