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Thanks to M. Riley for baculovirus expression support and J. Pack, M. Ellis and L. LeBrun for thoughtful comments on the manuscript. This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357.
The authors are, or have been, employees or contractors of Celgene and/or Bristol-Myers Squibb.
Peer review information Katarzyna Marcinkiewicz was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
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Extended Data Fig. 1 Sample electron density from the cereblon-DDB1-SALL4 ZF2-pomalidomide crystal structure.
Fo-Fc electron density map calculated in the absence of pomalidomide and contoured at 3σ following a round of refinement. Protein atoms are not shown in the figure.
Extended Data Fig. 2 SALL4 binding defects upon cereblon mutation mapped onto the structure of cereblon.
The surface of cereblon is colored by the effect of alanine mutations at that position on SALL4 ternary complex formation (blue, untested; green, no effect; red, detrimental effect; magenta, increased complex formation).
Structural model of SALL4 recruitment by pomalidomide to the cereblon-CRL4 E3 ligase complex, positioning SALL4 nearby the site of E2 recruitment on RBX11.
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Matyskiela, M.E., Clayton, T., Zheng, X. et al. Crystal structure of the SALL4–pomalidomide–cereblon–DDB1 complex. Nat Struct Mol Biol 27, 319–322 (2020). https://doi.org/10.1038/s41594-020-0405-9
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