Correction to: Nature Methods, published online 26 October 2023.

In the version of the article initially published, in Fig. 3c, d and g, the central canal was mislabeled as a blood vessel. In the fourth paragraph of the introduction, first and third paragraphs of the “High-specificity SBS imaging in live zebrafish and C. elegans” section and the Fig. 3 legend, mentions of blood vessels have been amended to discuss the spinal chord or central canal regions. Additionally, reference 28 has been changed to Thouvenin, O. et al. Origin and role of the cerebrospinal fluid bidirectional flow in the central canal. eLife (2020). These corrections have been made to the HTML and PDF versions of the article.