Leveraging nanopore long-read sequencing, scNanoHi-C identifies multiway interactions between enhancers and their target promoters within a single cell. Compared with short-read-based single-cell Hi-C or population-based multiway sequencing methods, scNanoHi-C offers new opportunities to investigate the heterogeneities of single-cell gene regulation networks mediated by high-order 3D chromatin structures.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Oudelaar, A. M. & Higgs, D. R. The relationship between genome structure and function. Nat. Rev. Genet. 22, 154–156 (2021). This review presents the relationship between genome structure and its function in gene regulation.
Deshpande, A. S. et al. Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing. Nat. Biotechnol. 40, 1488–1499 (2022). This paper reports a population-based high-order concatemer sequencing method based on the Oxford Nanopore platform.
Arrastia, M. V. et al. Single-cell measurement of higher-order 3D genome organization with scSPRITE. Nat. Biotechnol. 40, 64–73 (2022). This paper reports a single-cell higher-order chromatin sequencing method based on a split-and-pool strategy.
Nollmann, M., Bennabi, I., Gotz, M. & Gregor, T. The impact of space and time on the functional output of the genome. Cold Spring Harb. Perspect. Biol. 14, a040378 (2022). This review presents advances in microscopy for transcription, including the measurements of enhancer-promoter distance.
Lin, X. Q. et al. Nested epistasis enhancer networks for robust genome regulation. Science 377, 1077–1085 (2022). This paper reports synergistic effects between ultralong-distance enhancers.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Li, W. et al. scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells. Nat. Methods https://doi.org/10.1038/s41592-023-01978-w (2023).
Rights and permissions
About this article
Cite this article
scNanoHi-C uncovers single-cell high-order chromatin structures and gene regulation. Nat Methods 20, 1456–1457 (2023). https://doi.org/10.1038/s41592-023-01979-9
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-023-01979-9
