A new single-objective light-sheet microscope has been developed that uses novel optics and imaging protocols to increase resolution without compromising imaging speed and volume.
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References
Dunsby, C. Optically sectioned imaging by oblique plane microscopy. Optics Express 16, 20306–20316 (2008). Seminal paper that presents oblique plane microscopy (OPM), the first demonstration of single-objective light-sheet microscopy with no additional reflecting elements.
Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms. Nat. Photonics 9, 113–119 (2015). This paper reports SCAPE, a single-objective light-sheet microscope that allows volumetric imaging of living samples at ultrafast speeds.
Yang, B. et al. Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution. Nat. Methods 16, 501–504 (2019). This work solved the long-standing resolution-loss problem in OPM and demonstrated uncompromised spatial resolution for subcellular imaging.
Millett-Sikking, A. et al. High NA single-objective light-sheet. https://andrewgyork.github.io/high_na_single_objective_lightsheet/ (accessed 28 Jan 2022). An ongoing project that reports the use of a custom glass-tipped objective lens for high-resolution, single-objective light-sheet microscopy — a critical step towards widespread adoption.
Sapoznik, E. et al. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Elife 9, e57681 (2020). Demonstrates high-resolution, subcellular single-objective light-sheet imaging of living specimens mounted in a variety of imaging chambers, including microfluidic devices.
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This is a summary of: Yang, B. et al. DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy. Nat. Methods https://doi.org/10.1038/s41592-022-01417-2 (2022)
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DaXi: pushing the limits of single-objective light-sheet microscopy. Nat Methods 19, 414–415 (2022). https://doi.org/10.1038/s41592-022-01420-7
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DOI: https://doi.org/10.1038/s41592-022-01420-7
