Imaging whole nervous systems: insights into behavior from worms to fish

The development of systems combining rapid volumetric imaging with three-dimensional tracking has enabled the measurement of brain-wide dynamics in freely behaving animals such as worms, flies, and fish. These advances provide an exciting opportunity to understand the organization of neural circuits in the context of voluntary and natural behaviors. In this Comment, we highlight recent progress in this burgeoning area of research.

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References

  1. 1.

    Nguyen, J. P. et al. Proc. Natl. Acad. Sci. USA 113, E1074–E1081 (2016).

    CAS  Article  Google Scholar 

  2. 2.

    Venkatachalam, V. et al. Proc. Natl. Acad. Sci. USA 113, E1082–E1088 (2016).

    CAS  Article  Google Scholar 

  3. 3.

    Cong, L. et al. eLife 6, e28158 (2017).

    Article  Google Scholar 

  4. 4.

    Kim, D. H. et al. Nat. Methods 14, 1107–1114 (2017).

    CAS  Article  Google Scholar 

  5. 5.

    Symvoulidis, P. et al. Nat. Methods 14, 1079–1082 (2017).

    CAS  Article  Google Scholar 

  6. 6.

    Fosque, B. F. et al. Science 347, 755–760 (2015).

    CAS  Article  Google Scholar 

  7. 7.

    Ahrens, M. B. et al. Nature 485, 471–477 (2012).

    CAS  Article  Google Scholar 

  8. 8.

    Kato, S. et al. Cell 163, 656–669 (2015).

    CAS  Article  Google Scholar 

  9. 9.

    Schrödel, T., Prevedel, R., Aumayr, K., Zimmer, M. & Vaziri, A. Nat. Methods 10, 1013–1020 (2013).

    Article  Google Scholar 

  10. 10.

    Naumann, E. A. et al. Cell 167, 947–960 (2016).

    CAS  Article  Google Scholar 

  11. 11.

    Abrahamsson, S. et al. Biomed. Opt. Express 7, 855–869 (2016).

    CAS  Article  Google Scholar 

  12. 12.

    Dunn, T. W. et al. Neuron 89, 613–628 (2016).

    CAS  Article  Google Scholar 

  13. 13.

    Kawashima, T., Zwart, M. F., Yang, C. T., Mensh, B. D. & Ahrens, M. B. Cell 167, 933–946 (2016).

    CAS  Article  Google Scholar 

  14. 14.

    Chhetri, R. K. et al. Nat. Methods 12, 1171–1178 (2015).

    CAS  Article  Google Scholar 

  15. 15.

    Bouchard, M. B. et al. Nat. Photonics 9, 113–119 (2015).

    CAS  Article  Google Scholar 

  16. 16.

    Karagyozov, D., Mihovilovic Skanata, M., Lesar, A. & Gershow, M. Cell Rep. 25, 1371–1383 (2018).

    CAS  Article  Google Scholar 

  17. 17.

    Friedrich, J. et al. PLoS Comput. Biol. 13, e1005685 (2017).

    Article  Google Scholar 

  18. 18.

    Friedrich, J., Zhou, P. & Paninski, L. PLoS Comput. Biol. 13, e1005423 (2017).

    Article  Google Scholar 

  19. 19.

    Nguyen, J. P., Linder, A. N., Plummer, G. S., Shaevitz, J. W. & Leifer, A. M. PLoS Comput. Biol. 13, e1005517 (2017).

    Article  Google Scholar 

  20. 20.

    Day, R. N. & Davidson, M. W. Chem. Soc. Rev. 38, 2887–2921 (2009).

    CAS  Article  Google Scholar 

  21. 21.

    Livet, J. et al. Nature 450, 56–62 (2007).

    CAS  Article  Google Scholar 

  22. 22.

    Weissman, T. A. & Pan, Y. A. Genetics 199, 293–306 (2015).

    Article  Google Scholar 

  23. 23.

    Hobert, O., Glenwinkel, L. & White, J. Curr. Biol. 26, R1197–R1203 (2016).

    CAS  Article  Google Scholar 

  24. 24.

    Jenett, A. et al. Cell Rep. 2, 991–1001 (2012).

    CAS  Article  Google Scholar 

  25. 25.

    Marquart, G. D. et al. Front. Neural Circuits 9, 78 (2015).

    Article  Google Scholar 

  26. 26.

    Kabra, M., Robie, A. A., Rivera-Alba, M., Branson, S. & Branson, K. Nat. Methods 10, 64–67 (2013).

    CAS  Article  Google Scholar 

  27. 27.

    Berman, G. J., Bialek, W. & Shaevitz, J. W. Proc. Natl. Acad. Sci. USA 113, 11943–11948 (2016).

    CAS  Article  Google Scholar 

  28. 28.

    Liu, M., Sharma, A. K., Shaevitz, J. W. & Leifer, A. M. eLife 7, e36419 (2018).

    Article  Google Scholar 

  29. 29.

    Dupre, C. & Yuste, R. Curr. Biol. 27, 1085–1097 (2017).

    CAS  Article  Google Scholar 

  30. 30.

    Chartier, T. F., Deschamps, J., Dürichen, W., Jékely, G. & Arendt, D. Open Biol. 8, 180139 (2018).

    Article  Google Scholar 

  31. 31.

    Knott, G. J. & Doudna, J. A. Science 361, 866–869 (2018).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Aravinthan D. T. Samuel.

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Calarco, J.A., Samuel, A.D.T. Imaging whole nervous systems: insights into behavior from worms to fish. Nat Methods 16, 14–15 (2019). https://doi.org/10.1038/s41592-018-0276-8

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