The requirement for a protospacer adjacent motif (PAM) is a well-known limitation of the CRISPR–Cas9 system, as it restricts the range of sequences that can be targeted. To address this limitation, we demonstrate a phage-assisted evolution approach for engineering a compact SlugCas9, simplifying its PAM requirement and broadening its DNA targeting scope.
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References
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This is a summary of: Qi, T. et al. Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM. Nat. Chem. Biol. https://doi.org/10.1038/s41589-023-01481-5 (2023).
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Expanding the genome-targeting scope of a compact Cas9 using phage-assisted evolution. Nat Chem Biol 20, 275–276 (2024). https://doi.org/10.1038/s41589-023-01482-4
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DOI: https://doi.org/10.1038/s41589-023-01482-4
