Many questions on the activity of the Ras proto-oncogene are unanswered due to the lack of tools for detecting active Ras in living cells. Here, we used protein design and structure prediction algorithms to develop biosensors that detect the activity and environment of endogenous Ras.
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References
Chiu, V. K. et al. Ras signalling on the endoplasmic reticulum and the Golgi. Nat. Cell Biol. 4, 343–350 (2002). This paper describes one of the first tools for measuring Ras activity.
Mochizuki, N. et al. Spatiotemporal images of growth-factor-induced activation of Ras and Rap1. Nature 411, 1065–1068 (2001). This paper describes one of the first sensors for Ras activity.
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This is a summary of: Zhang, J. Z. et al. Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-02107-w (2024).
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Computer-based design of sensors that can monitor endogenous Ras activity. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-023-02108-9
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DOI: https://doi.org/10.1038/s41587-023-02108-9
