A systematic examination of eight different single-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) technologies revealed marked differences in the complexity of sequencing libraries and the specificity of DNA tagmentation that they achieve. Our pipeline for universal mapping of scATAC-seq data (PUMATAC) allowed a fair benchmarking of existing methods and enables the seamless integration of future datasets and technologies.
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References
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This is a summary of: De Rop, F. V. et al. Systematic benchmarking of single-cell ATAC-sequencing protocols. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01881-x (2023).
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Benchmarking of single-cell ATAC sequencing tools. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01897-3
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DOI: https://doi.org/10.1038/s41587-023-01897-3
