Nano-tRNAseq is a nanopore-based, cost-effective and high-throughput approach to quantify transfer RNA (tRNA) abundances and modifications simultaneously, providing a framework to study the ‘tRNAome’ at single-molecule resolution. We envision that Nano-tRNAseq will enable us to study the role of tRNA molecules and their modifications in a wide variety of contexts.
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This is a summary of: Lucas, M. C. et al. Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01743-6 (2023).
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Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01755-2