Sequencing individual cells in a sample enables scientists to infer the unique characteristics of important subsets. Single-cell sequencing methods that rely on microfluidics for cell barcoding are limited in speed, scale and flexibility. We developed a technique that uses particle-templated emulsification instead of microfluidics and can process millions of cells within minutes.
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References
Klein, A. M. et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161, 1187–1201 (2015). A research article presenting a high-throughput single-cell RNA sequencing (scRNA-seq) technique that utilizes custom droplet-based microfluidics and in-droplet reverse transcription.
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This is a summary of: Clark, I. C. et al. Microfluidics-free single-cell genomics with templated emulsification. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01685-z (2023)
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The next generation of single-cell sequencing methods can be microfluidics-free. Nat Biotechnol 41, 1524–1525 (2023). https://doi.org/10.1038/s41587-023-01710-1
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DOI: https://doi.org/10.1038/s41587-023-01710-1
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