The global microbiota contains an enormous, previously inaccessible reservoir of biodiversity that can now be captured as large DNA fragments in clone libraries. To mine cloned biodiversity more effectively, we developed a CRISPR-interference-based counter-selection interruption circuit for the high-throughput retrieval of desired clones within complex libraries.
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This is a summary of: Burian, J. et al. High-throughput retrieval of target sequences from complex clone libraries using CRISPRi. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01531-8 (2022).
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Rapid mining of (meta)genomic biodiversity by repurposing CRISPRi for positive selection. Nat Biotechnol 41, 620–621 (2023). https://doi.org/10.1038/s41587-022-01563-0
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DOI: https://doi.org/10.1038/s41587-022-01563-0
