Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

IMAGING

Light-sheet microscopy at high resolution

An improved light-sheet microscope images live cells at sub-100-nm axial resolution.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Three-dimensional interferometric lattice light-sheet imaging.

References

  1. 1.

    Cao, B., Wang, G., Li, J. & Pertsinidis, A. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01042-y (2021).

  2. 2.

    Hell, S. W., Stelzer, E. H. K., Lindek, S. & Cremer, C. Opt. Lett. 19, 222–224 (1994).

    CAS  Article  Google Scholar 

  3. 3.

    Gustafsson, M. G., Agard, D. A. & Sedat, J. W. J. Microsc. 195, 10–16 (1999).

    CAS  Article  Google Scholar 

  4. 4.

    Shtengel, G. et al. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).

    CAS  Article  Google Scholar 

  5. 5.

    Chen, B.-C. et al. Science 346, 1257998 (2014).

    Article  Google Scholar 

  6. 6.

    Chang, B.-J., Dean, K. M. & Fiolka, R. Opt. Express 28, 27052–27077 (2020).

    Article  Google Scholar 

  7. 7.

    Hell, S. W., Lindek, S. & Stelzer, E. H. K. J. Mod. Opt. 41, 675–681 (1994).

    Article  Google Scholar 

  8. 8.

    Shao, L. et al. Biophys. J. 94, 4971–4983 (2008).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The author is grateful for funding by the National Institutes of Health (R35GM133522) and thanks James Manton for help simulating the 4Pi point spread function used in Fig. 1b,d.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Reto Fiolka.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Fiolka, R. Light-sheet microscopy at high resolution. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01101-4

Download citation

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing