Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

IMAGING

Light-sheet microscopy at high resolution

Nature Biotechnology volume 39, pages 1345–1346 (2021)Cite this article

Subjects

An improved light-sheet microscope images live cells at sub-100-nm axial resolution.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Three-dimensional interferometric lattice light-sheet imaging.

References

  1. Cao, B., Wang, G., Li, J. & Pertsinidis, A. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01042-y (2021).

  2. Hell, S. W., Stelzer, E. H. K., Lindek, S. & Cremer, C. Opt. Lett. 19, 222–224 (1994).

    Article  CAS  Google Scholar 

  3. Gustafsson, M. G., Agard, D. A. & Sedat, J. W. J. Microsc. 195, 10–16 (1999).

    Article  CAS  Google Scholar 

  4. Shtengel, G. et al. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).

    Article  CAS  Google Scholar 

  5. Chen, B.-C. et al. Science 346, 1257998 (2014).

    Article  Google Scholar 

  6. Chang, B.-J., Dean, K. M. & Fiolka, R. Opt. Express 28, 27052–27077 (2020).

    Article  Google Scholar 

  7. Hell, S. W., Lindek, S. & Stelzer, E. H. K. J. Mod. Opt. 41, 675–681 (1994).

    Article  Google Scholar 

  8. Shao, L. et al. Biophys. J. 94, 4971–4983 (2008).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The author is grateful for funding by the National Institutes of Health (R35GM133522) and thanks James Manton for help simulating the 4Pi point spread function used in Fig. 1b,d.

Author information

Authors and Affiliations

  1. Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Reto Fiolka

Authors
  1. Reto Fiolka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Reto Fiolka.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fiolka, R. Light-sheet microscopy at high resolution. Nat Biotechnol 39, 1345–1346 (2021). https://doi.org/10.1038/s41587-021-01101-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41587-021-01101-4

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing