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Guidelines for reporting single-cell RNA-seq experiments

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Fig. 1: Typical design steps of single-cell transcriptomics experiments with examples for each step.
Fig. 2: Overview of the main components of a minSCe study.


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This work was supported by the European Molecular Biology Laboratory; the Wellcome Trust Biomedical Resources grant Single Cell Gene Expression Atlas (108437/Z/15/Z); the California Institute for Regenerative Medicine (GC1R-06673-B); the Chan Zuckerberg Initiative DAF, an advised fund of the Silicon Valley Community Foundation (2018–182730); and the NIH Common Fund, through the Office of Strategic Coordination/Office of the NIH Director (OT2OD026677).

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Authors and Affiliations



A.F., N. George, M.G., P.N., B.A., S.K.F., C.F., M.A.F., L.H., D.T., N.V. and N.M. curated data and developed metadata standards; L.C., J.K., N.M., R.H.S., A.B. and I.P. supervised the project and engaged with the larger community; S.T. and J.M. provided support and evaluation of the standards and contributed to the manuscript; A.F., N. George, M.G., P.N., B.A., S.K.F., C.F., M.A.F., L.C., N. Gehlenborg, L.H., R.H.S., D.T., N.V., A.B. and I.P. wrote the manuscript.

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Correspondence to Anja Füllgrabe or Irene Papatheodorou.

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The authors declare no competing interests.

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Editorial note: This article has been peer reviewed.

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Supplementary Information

Supplementary Note including Supplementary Tables 1–7 and Supplementary Figs. 1 and 2.

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Füllgrabe, A., George, N., Green, M. et al. Guidelines for reporting single-cell RNA-seq experiments. Nat Biotechnol 38, 1384–1386 (2020).

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