Extended Data Fig. 5: PAGERTF detects a wide range of antigens (Related to Fig. 2a).
From: Synthetic GPCRs for programmable sensing and control of cell behaviour
a, PAGERTF antigen response assay. Same as Fig. 2a, but including comparisons of cognate antigen to two non-cognate antigens for each PAGERTF. The antigens used and their concentrations are labeled above each bar. Plots are mean ± s.d. for 3 technical replicates per condition, n = 1 independent experiment. b, Five nanobodies that bind to the receptor binding domain (RBD) of SARS-CoV2 spike protein as shown (sequences in Supplementary Information) were tested in PAGERTF. c, HEK cells expressing the indicated PAGERTF were treated with 200 nM spike protein and 500 nM SalB for 15 min before luciferase measurement 8 h later. Plots are mean ± s.d. for 3 technical replicates per condition, representing n = 2 independent experiment. p-values were determined using two-way ANOVA with Tukey’s multiple comparisons test to adjust for multiple comparisons. ****, p < 0.0001.
