Extended Data Fig. 7: Validation of ERα as import shuttle for FKBP12F36V.
From: Targeted protein relocalization via protein transport coupling
a. TRAM engaging ERα and FKBP12F36V. b, FKBP12F36V nuclear import dose response derived from the analysis pipeline after a 3-hour treatment with 5 engaging ERα. c, Representative live-cell images of ERα-dependent FKBP12F36V import upon treatment with 5 or unlinked warheads 3 and 10 for 3 h. d, Nuclear localization of mCherry-FKBP12F36V after treatment with 5 or unlinked warheads 3 and 10 for 3 h. e, Nuclear localization of ERα after treatment with 5 or unlinked warheads 3 and 10 for 3 h. f, Representative live-cell images of the unlinked warhead competition assay for ERα-dependent FKBP12F36V import for 3 h. g, mCherry localization upon treatment with 5 in competition with either 1x or 10x of 3 or 10 for 3 h. h, Nuclear localization of ERα upon treatment with 5 in competition with either 1x or 10x of 3 or 10 for 3 h. Images in c, f, are representative of three biological replicates. Data in b, d, e, g, h are mean ± s.d. of three independent experiments. Scale bars are 20 µm. P values in b, d, e, g, h were determined by one-way ANOVA with Dunnett’s post hoc test comparing each condition to the DMSO control. Hoechst stains the nucleus.
