Neural circuitry linking mating and egg laying in Drosophila females

Abstract

Mating and egg laying are tightly cooordinated events in the reproductive life of all oviparous females. Oviposition is typically rare in virgin females but is initiated after copulation. Here we identify the neural circuitry that links egg laying to mating status in Drosophila melanogaster. Activation of female-specific oviposition descending neurons (oviDNs) is necessary and sufficient for egg laying, and is equally potent in virgin and mated females. After mating, sex peptide—a protein from the male seminal fluid—triggers many behavioural and physiological changes in the female, including the onset of egg laying1. Sex peptide is detected by sensory neurons in the uterus2,3,4, and silences these neurons and their postsynaptic ascending neurons in the abdominal ganglion5. We show that these abdominal ganglion neurons directly activate the female-specific pC1 neurons. GABAergic (γ-aminobutyric-acid-releasing) oviposition inhibitory neurons (oviINs) mediate feed-forward inhibition from pC1 neurons to both oviDNs and their major excitatory input, the oviposition excitatory neurons (oviENs). By attenuating the abdominal ganglion inputs to pC1 neurons and oviINs, sex peptide disinhibits oviDNs to enable egg laying after mating. This circuitry thus coordinates the two key events in female reproduction: mating and egg laying.

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Fig. 1: oviDNs control oviposition.
Fig. 2: oviDNs induce the oviposition motor sequence with equal potency in virgin and mated females.
Fig. 3: pC1 neurons suppress egg laying and oviDN activity and are regulated by the sex-peptide pathway.
Fig. 4: oviDNs integrate mating status and substrate signals through distinct upstream pathways.

Data availability

The datasets generated during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank the Janelia FlyLight, Fly Facility, Project Technical Resources, Molecular Biology, Functional Connectome and Experimental Technology teams for technical assistance; A. Edmonson-Stait and G. Jefferis for initial tracing of one of the oviIN cells; U. Heberlein, K. Feng and V. Vijayan for comments on the manuscript; and V. Vijayan and G. Maimon for sharing preliminary oviDN calcium-imaging data. This work was funded by the Howard Hughes Medical Institute.

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Authors

Contributions

B.J.D., F.W. and K.W. conceived the study and wrote the manuscript. F.W. and K.W. performed all experiments and analysed the data. N.F., C.P., T.Y., F.W. and R.P. reconstructed selected neurons and synapses in the FAFB electron microscopy volume, which was provided before publication by D.B.

Corresponding author

Correspondence to Barry J. Dickson.

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The authors declare no competing interests.

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Peer review informationNature thanks Rebecca Yang and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Extended data figures and tables

Extended Data Fig. 1 Split-GAL4 driver lines targeting oviDNs, SPSNs, SAG neurons, pC1 neurons, oviENs and oviINs.

Confocal images of the central nervous system from female and male flies carrying the indicated split-GAL4 driver lines as well as UAS-myrFLAG or UAS-CsChrimson-mVenus. Samples were stained with the monoclonal antibody nc82 to reveal all synapses (magenta), and with anti-FLAG or anti-GFP to reveal the membranes of targeted neurons (green). Scale bar, 100 μm. Both oviDN-SS1 and oviDN-SS2 label a single oviDNa and a single oviDNb cell in each hemisphere; oviDN-SS2 also weakly labels an unrelated cell (pMP1) that is present in both sexes.

Extended Data Fig. 2 Expression of fru and dsx in oviDNs and pC1 neurons.

Confocal images of female brains showing the co-labelling of oviDN-SS lines with fru-LexA but not dsx-LexA, and of the pC1-SS1 line with dsx-LexA but not fru-LexA. Scale bars, 20 μm.

Extended Data Fig. 3 Neurotransmitter types revealed by fluorescence in situ hybridization.

Confocal images showing the expression of GAD1, ChAT and vGluT in oviDNs, SAG neurons, pC1 neurons, oviENs and oviINs in female brains. Red arrows indicate cell bodies of interest. Scale bars, 20 μm.

Extended Data Fig. 4 oviDNs are required for oviposition but not copulation.

a, Number of GFP-expressing neurons in female brains of the indicated genotypes. **P < 0.01 by Wilcoxon test. b, Total number of eggs laid by individual mated females over 10 consecutive days after mating, shown as mean ± s.e.m. Note the values of zero for both oviDN-ablated genotypes at all time points. ***P < 0.001 by Wilcoxon test. c, Cumulative traces showing the percentage of females copulating over a 30-min observation period.

Extended Data Fig. 5 Stochastic labelling and unsilencing of oviDNs.

a, Images of two female samples in which a single oviDNa or oviDNb cell is labelled, as shown in Fig. 1b. Arrowheads indicate branches that are present in oviDNb (solid) but absent in oviDNa (open). The branch that is labelled by arrowhead 1 was primarily used to distinguish oviDNa from oviDNb. b, Example images of brains in which oviDNs were either silenced (red; Kir2.1::tdTomato) or unsilenced (green; mCD8::GFP). The number of unsilenced oviDNs in each sample is shown. Green arrowheads indicate distinctive branches of oviDNb. Brains were counterstained with nc82 (blue). Scale bar, 100 μm. c, Number of eggs laid in the five days after mating by mated females with different oviDNs unsilenced. ***P < 0.001, **P < 0.01 by Wilcoxon test. Scatter plots show mean ± s.e.m. d, Confocal images of two samples in which a single oviDN was loaded with neurobiotin during whole-cell recording. The samples were stained with streptavidin (to reveal the recorded cell, yellow) and nc82 (blue). Arrowheads indicate oviDNb-specific branches. Scale bars, 100 μm.

Extended Data Fig. 6 Sequence of oviposition actions after oviDN stimulation.

Example ethograms showing the onsets of oviposition actions in mated females after photoactivation (3 s) of oviDNs at varying light intensities. Each row represents a single female.

Extended Data Fig. 7 Anatomical and functional characterization of pC1 neurons.

a. Confocal images of single pC1 neurons in the female brain, as shown in Fig. 3d. Arrowheads indicate the presence (solid) or absence (open) of subtype-specific branches. b, Confocal images of neurobiotin-filled pC1 neurons from which whole-cell patch recordings were obtained, indicating the branches that were used for subtype identification as in a. c, d, Number of eggs laid by virgin females during a one-hour (c) or three-day (d) period in which either SAG or pC1 neurons were optogenetically silenced. e, Basal GCaMP6s signals in pC1 cell bodies in virgin and mated females. ***P < 0.001 by Wilcoxon test; scatter plots show mean ± s.e.m. (d, e).

Extended Data Fig. 8 Egg-laying substrate preferences and substrate-evoked calcium responses in oviDNs, oviENs and oviINs.

a, Image of the egg-laying chambers in each of which an individual mated female had laid numerous eggs. Chambers with plain agarose (blue box), agarose containing 150 mM sucrose (red box) and plastic surface (green box) are indicated. b, Total number of eggs laid by individual mated females in a 12-h observation period. **P < 0.01, ***P < 0.001 by Wilcoxon test. c, Preference indices showing the preference of female flies for laying eggs on different substrates. Preference index (PI) is calculated as (number of eggs on plain agarose – number of eggs on other substrate)/total number of eggs. Data are mean ± s.e.m. d, Projected images of oviDNs (top), oviENs (middle) and oviINs (bottom) expressing GCaMP6s, showing ROIs for quantification. e, Example ΔF/F0 traces for each ROI upon presentation of the indicated substrates, in virgin (left) and mated (right) females. Horizontal bars indicate presentation of the substrate. Darker traces are averaged from six trials (lighter traces).

Extended Data Table 1 Synaptic connections identified by electron microscopy reconstruction
Extended Data Table 2 oviDN inputs identified by electron microscopy reconstruction

Supplementary information

41586_2020_2055_MOESM3_ESM.mov

: Optogenetic activation of oviDNs elicits oviposition behaviour. A montage of video clips of 16 mated oviDN-SS2 UAS-Chrimson females upon 5 s of continuous 625 nm illumination at 200 µW/mm2), shown at half speed (100 fps).

41586_2020_2055_MOESM4_ESM.mov

: oviDNs reconstructed in female brain EM volume. Two oviDNa cells (blue and green) and one oviDNb cell (red) reconstructed in the right hemisphere of the FAFB EM volume.

41586_2020_2055_MOESM5_ESM.mov

: pC1 neurons reconstructed in female brain EM volume. Five pC1 neurons reconstructed in the right hemisphere of the FAFB EM volume. pC1a, pC1c, and pC1e were fully traced; pC1b and pC1d were only partially reconstructed.

41586_2020_2055_MOESM6_ESM.mov

: SAG neurons are presynaptic to pC1a neurons. A pair of SAG neurons (blue and green) partially reconstructed in the FAFB EM volume, and one fully-traced pC1a neuron (red) in the right hemisphere. The location of synapses between SAG and pC1a neurons are indicated by yellow or cyan balls.

41586_2020_2055_MOESM7_ESM.mov

: oviEN and oviIN are presynaptic to oviDNs. oviDN (blue), oviEN (pink), oviIN (green) and pC1neurons (red) reconstructed in the FAFB EM volume in the right hemisphere.

Supplementary Information

This file contains Supplementary Tables 1 and 2.

Reporting Summary

Video 1

: Optogenetic activation of oviDNs elicits oviposition behaviour. A montage of video clips of 16 mated oviDN-SS2 UAS-Chrimson females upon 5 s of continuous 625 nm illumination at 200 µW/mm2), shown at half speed (100 fps).

Video 2

: oviDNs reconstructed in female brain EM volume. Two oviDNa cells (blue and green) and one oviDNb cell (red) reconstructed in the right hemisphere of the FAFB EM volume.

Video 3

: pC1 neurons reconstructed in female brain EM volume. Five pC1 neurons reconstructed in the right hemisphere of the FAFB EM volume. pC1a, pC1c, and pC1e were fully traced; pC1b and pC1d were only partially reconstructed.

Video 4

: SAG neurons are presynaptic to pC1a neurons. A pair of SAG neurons (blue and green) partially reconstructed in the FAFB EM volume, and one fully-traced pC1a neuron (red) in the right hemisphere. The location of synapses between SAG and pC1a neurons are indicated by yellow or cyan balls.

Video 5

: oviEN and oviIN are presynaptic to oviDNs. oviDN (blue), oviEN (pink), oviIN (green) and pC1neurons (red) reconstructed in the FAFB EM volume in the right hemisphere.

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Wang, F., Wang, K., Forknall, N. et al. Neural circuitry linking mating and egg laying in Drosophila females. Nature 579, 101–105 (2020). https://doi.org/10.1038/s41586-020-2055-9

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