Transfection is a foundational technology in biosciences, biotechnology, and the development of biotherapeutic agents. Given its transcendence, it is problematic that transfection remains a poorly understood and insufficiently characterized endeavour. Here we state the case for the urgent reworking of transfection as a fit-for-purpose technological foundation for the ambitions of scientists and engineers.
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References
Eisenstein, M. Enzymatic DNA synthesis enters new phase. Nat. Biotechnol. 38, 1113–1115 (2020).
Lipps, C. et al. Eternity and functionality — rational access to physiologically relevant cell lines. Biol. Chem. 394, 1637–1648 (2013).
Durymanov, M. & Reineke, J. Non-viral delivery of nucleic acids: insight into mechanisms of overcoming intracellular barriers. Front. Pharmacol. 9, 971 (2018).
Mancinelli, S. et al. Design of transfections: implementation of design of experiments for cell transfection fine-tuning. Biotechnol. Bioeng. 118, 4488–4502 (2021).
Kim, T. K. & Eberwine, J. H. Mammalian cell transfection: the present and the future. Anal. Bioanal. Chem. 397, 3173–3178 (2010).
Chong, Z. X., Yeap, S. K. & Ho, W. Y. Transfection types, methods and strategies: a technical review. PeerJ. 9, e11165 (2021).
Fus-Kujawa, A. et al. An overview of methods and tools for transfection of eukaryotic cells in vitro. Front. Bioeng. Biotechnol. 9, 701031 (2021).
Di Blasi, R. et al. A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation. Nat. Commun. 12, 2545 (2021).
Synthego. An Inside Look at What’s Happening at the Benchtop - CRISPR benchmark report, Vol. 01 https://www.synthego.com/crispr-benchmark (accessed 13 April 2023).
Faruqui, N. et al. Cellular metrology: scoping for a value proposition in extra- and intracellular measurements. Front. Bioeng. Biotechnol. 7, 456 (2020).
Acknowledgements
This work was supported in part by the UK Centre for Mammalian Synthetic Biology (Biotechnology & Biological Sciences Research Council, BB/M018040/1).
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Wells-Holland, C., Elfick, A. Transfection reflections: fit-for-purpose delivery of nucleic acids. Nat Rev Mol Cell Biol 24, 771–772 (2023). https://doi.org/10.1038/s41580-023-00627-6
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DOI: https://doi.org/10.1038/s41580-023-00627-6
