Cellular protrusions are highly dynamic structures that facilitate cell–cell communication and are increasingly recognised for their roles in the shedding of bioactive extracellular vesicles (EVs). The intrinsic and extrinsic mechanisms that govern the shedding of EVs from cellular protrusions and their potential physiological roles are beginning to emerge.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
van Niel, G. et al. Challenges and directions in studying cell-cell communication by extracellular vesicles. Nat. Rev. Mol. Cell Biol. 23, 369–382 (2022).
Scita, G., Confalonieri, S., Lappalainen, P. & Suetsugu, S. IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions. Trends Cell Biol. 18, 52–60 (2008).
Nishimura, T. et al. Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells. Dev. Cell 56, 842–859.e8 (2021).
de Poret, A. et al. Extracellular vesicles containing the I-BAR protein IRSp53 are released from the cell plasma membrane in an Arp2/3 dependent manner. Biol. Cell https://doi.org/10.1111/boc.202100095 (2022).
Gaeta, I. M., Meenderink, L. M., Postema, M. M., Cencer, C. S. & Tyska, M. J. Direct visualization of epithelial microvilli biogenesis. Curr. Biol. 31, 2561–2575.e2566 (2021).
Hurbain, I. et al. Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development. Curr. Biol. 32, 361–373.e366 (2022).
Thamm, K. et al. Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic 20, 39–60 (2019).
Inamdar, K. et al. Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53. eLife https://doi.org/10.7554/eLife.67321 (2021).
Vinay, L. & Belleannée, C. EV duty vehicles: Features and functions of ciliary extracellular vesicles. Front. Genet. 13, 916233 (2022).
Mathieu, M. et al. Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat. Commun. 12, 4389 (2021).
Acknowledgements
We thank N. Gov at Weizmann Institute, R. Sorkin at Tel Aviv University, and M. Kozlov at Tel Aviv University and lab members for insightful discussions. We sincerely apologize to the many authors whom we could not cite due to space limitations. This work was supported by the Fondation pour la Recherche Medicale (FRM 2020-2023), Institut Curie and the Centre National de la Recherche Scientifique (CNRS) to G.A. and G.R., Japan Society for Promotion of Science (KAKENHI, JP 20H03252, JP20KK0341, JP21H05047) to SS., Japan Society for Promotion of Science (JP20K06625) to T.N., and Japan Science and Technology cooperation CREST (JPMJCR1863) to S.S.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
D’Angelo, G., Raposo, G., Nishimura, T. et al. Protrusion-derived vesicles: new subtype of EVs?. Nat Rev Mol Cell Biol 24, 81–82 (2023). https://doi.org/10.1038/s41580-022-00555-x
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41580-022-00555-x