DNA double-strand breaks (DSBs) are fundamental to cell biology, from evolution to the latest gene-editing technologies. Yet, does an assay exist that truly quantitatively visualizes DSBs? Over-reliance on DSB detection by proxies can misguide interpretation of conventional assays, and more faithful DSB representatives await development.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Scully, R. et al. DNA double-strand break repair-pathway choice in somatic mammalian cells. Nat. Rev. Mol. Cell Biol. 20, 698–714 (2019).
Mah, L. J. et al. γH2AX: a sensitive molecular marker of DNA damage and repair. Leukemia 24, 679–686 (2010).
Rybak, P. et al. Low level phosphorylation of histone H2AX on serine 139 (γH2AX) is not associated with DNA double-strand breaks. Oncotarget 7, 49574–49587 (2016).
Onn, L. et al. SIRT6 is a DNA double-strand break sensor. eLife 9, e51636 (2020).
Bouquet, F. et al. TGFbeta1 inhibition increases the radiosensitivity of breast cancer cells in vitro and promotes tumor control by radiation in vivo. Clin. Cancer Res. 17, 6754–6765 (2011).
Maciejowski, J. & de Lange, T. Telomeres in cancer: tumour suppression and genome instability. Nat. Rev. Mol. Cell Biol. 18, 175–186 (2017).
Shee, C. et al. Engineered proteins detect spontaneous DNA breakage in human and bacterial cells. eLife 2, e01222 (2013).
Yang, X. et al. Roles of SIRT6 in kidney disease: a novel therapeutic target. Cell Mol. Life Sci. 79, 53 (2021).
Kordon, M. M. et al. STRIDE — a fluorescence method for direct, specific in situ detection of individual single- or double-strand DNA breaks in fixed cells. Nucleic Acids Res. 48, e14-e14 (2019).
Galbiati, A. et al. A novel single-cell method provides direct evidence of persistent DNA damage in senescent cells and aged mammalian tissues. Aging Cell 16, 422–427 (2017).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Reviews Molecular Cell Biology thanks the anonymous reviewers for their contribution to the peer review of this work.
Rights and permissions
About this article
Cite this article
Atkinson, J., Bezak, E. & Kempson, I. Imaging DNA double-strand breaks — are we there yet?. Nat Rev Mol Cell Biol 23, 579–580 (2022). https://doi.org/10.1038/s41580-022-00513-7
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41580-022-00513-7
This article is cited by
-
Mitochondrial DNA is a key driver in cigarette smoke extract-induced IL-6 expression
Hypertension Research (2024)