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Mechano-bactericidal actions of nanostructured surfaces

Abstract

Antibiotic resistance is a global human health threat, causing routine treatments of bacterial infections to become increasingly difficult. The problem is exacerbated by biofilm formation by bacterial pathogens on the surfaces of indwelling medical and dental devices that facilitate high levels of tolerance to antibiotics. The development of new antibacterial nanostructured surfaces shows excellent prospects for application in medicine as next-generation biomaterials. The physico-mechanical interactions between these nanostructured surfaces and bacteria lead to bacterial killing or prevention of bacterial attachment and subsequent biofilm formation, and thus are promising in circumventing bacterial infections. This Review explores the impact of surface roughness on the nanoscale in preventing bacterial colonization of synthetic materials and categorizes the different mechanisms by which various surface nanopatterns exert the necessary physico-mechanical forces on the bacterial cell membrane that will ultimately result in cell death.

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Fig. 1: Comparison of biomimetic antibiofouling and mechano-bactericidal nanostructured surfaces.
Fig. 2: Two classes of mechano-bactericidal action of nanostructured surfaces.
Fig. 3: Differential attachment of bacteria to micro–nanostructured surfaces.
Fig. 4: Variation in the topographic dimensions of mechano-bactericidal surface nanopatterns.

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Acknowledgements

Funding from the Australian Research Council (ARC) Industrial Transformation Research Hubs scheme (project number IH130100017) and the ARC Industrial Transformation Training Centre scheme (project number IC180100005) to E.P.I. and NIH grant R01GM124436 to P.S. are gratefully acknowledged.

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D.P.L. and E.P.I. researched data for the article. P.S., D.P.L., S.J., V.A.B., R.J.C. and E.P.I. substantially contributed to discussion of content, wrote the article and reviewed and edited the manuscript before submission.

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Correspondence to Paul Stoodley or Elena P. Ivanova.

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Biophysical model of the cicada wing nanopillar-bacterial cell interactions: https://www.youtube.com/watch?v=KSdMYX4gqp8

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Glossary

Antibiofouling

Describes specially designed materials and coatings to prevent or remove biofouling by any number of organisms on wetted surfaces.

Hydrophobic

The physical property of having no affinity with water (that is, water repellent).

Bactericidal

Destroys bacteria.

Biocidal

The action of a chemical substance or microorganism to destroy a harmful organism or to prevent or control its effects.

Osseointegration

Functional bone adherence to the implant surface.

Osseoconduction

Guiding the reparative growth of the natural bone.

Micro–nanostructuring

A technique used to impart physical patterns onto a surface with surface features (protrusions or depressions) on the scale of micrometres or nanometres. The size and shapes of such patterns determine the overall topography of the surface at these scales. Such patterns can be made by depositing material onto a surface, removing material from a surface, or both.

Nanotopographies

The specific surface features that form or are generated at the nanoscale.

Surface roughness

Irregularities of the surface, either random or repetitive, that make up the 3D topography of the surface.

Nanoscale

Physical dimensions less than 1 μm.

Mechano-bactericidal

The action of natural or synthetic nanomaterials that can physically induce bacterial cell death through the application of physical forces.

Hydrophilic

The physical property of a molecule and/or surface the interactions of which with water are thermodynamically favourable; that is, it can form ionic or hydrogen bonds with water.

Microscale

Physical dimensions less than 1 mm.

Stretching modulus

Also known as Young’s modulus. The modulus of elasticity: a measure of the ability of a material to withstand changes in length when under lengthwise tension or compression.

Turgor pressure

The force (hydrostatic pressure) within the cell, caused by the osmotic flow of water, that pushes the cell membrane against the cell wall.

Biomimetic

Imitating the models, systems and elements found in nature.

Aspect ratio

The ratio of feature width to feature height. ‘High aspect ratio’ refers to a structure that is exceedingly tall in comparison with its width.

Cytotoxic

Causing cell damage or death.

Lipophilicity

The degree to which an organic molecule can dissolve in fat, oil or other non-polar solvents.

Amphiphilicity

The physical property of a molecule or compound that possesses both hydrophilic (water-loving) and hydrophobic (water-repellent) characteristics.

Biomaterials

Engineered substances that can be introduced into body tissues as part of an implanted medical, therapeutic or diagnostic device.

Biocompatibility

The ability of a biomaterial to perform its desired function without eliciting detrimental local or systemic effects when implanted within the body.

Cytocompatibility

Supportive of the common functions of all cells, including basal functions such as viability and proliferation as well as functions specific to the phenotype of each cell type defined by morphology of the cytoskeleton and expression of proteins and specific enzymes.

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Linklater, D.P., Baulin, V.A., Juodkazis, S. et al. Mechano-bactericidal actions of nanostructured surfaces. Nat Rev Microbiol 19, 8–22 (2021). https://doi.org/10.1038/s41579-020-0414-z

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