Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Nanopore sequencing

Unfolding the path to nanopore protein sequencing

Subjects

A modified nanopore enables enzyme-free threading of single polypeptides to detect post-translational modifications

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Protein threading through a nanopore detects post-translational modifications.

References

  1. Marx, V. Nat. Methods 20, 6–11 (2023).

    Article  CAS  Google Scholar 

  2. Quick, J. et al. Nature 530, 228–232 (2016).

    Article  CAS  Google Scholar 

  3. Martin-Baniandres, P. et al. Nat. Nanotechnol. https://doi.org/10.1038/s41565-023-01462-8 (2023).

    Article  Google Scholar 

  4. Wang, Y. Y., Zhao, Y., Bollas, A., Wang, Y. Y. & Au, K. F. Nat. Biotechnol. 39, 1348–1365 (2021).

    Article  CAS  Google Scholar 

  5. Goyal, P. et al. Nature 516, 250–253 (2014).

    Article  CAS  Google Scholar 

  6. Ouldali, H. et al. Nat. Biotechnol. 38, 176–181 (2020).

    Article  CAS  Google Scholar 

  7. Yu, L. et al. Nat. Biotechnol. 41, 1130–1139 (2023).

    Article  CAS  Google Scholar 

  8. Zhang, S. et al. Nat. Chem. 13, 1192–1199 (2021).

    Article  CAS  Google Scholar 

  9. Brinkerhoff, H., Kang, A. S. W., Liu, J., Aksimentiev, A. & Dekker, C. Science 374, 1509–1513 (2021).

    Article  CAS  Google Scholar 

  10. Reed, B. D. et al. Science 378, 186–192 (2022).

    Article  CAS  Google Scholar 

  11. Alfaro, J. A. et al. Nat. Methods 18, 604–617 (2021).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Stefan Howorka.

Ethics declarations

Competing interests

Stefan Howorka is inventor on a patent on a protein pore used by Oxford Nanopore Technologies plc for DNA sequencing.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dorey, A., Howorka, S. Unfolding the path to nanopore protein sequencing. Nat. Nanotechnol. 18, 1259–1260 (2023). https://doi.org/10.1038/s41565-023-01480-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41565-023-01480-6

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing