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Single-molecule imaging goes high throughput

Combining single-molecule localization microscopy with flow cytometry and three-dimensional localization enables the high throughput and precise localization of fluorescent molecules in live flowing cells.

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Fig. 1: Single-molecule localization imaging in flowing cells.

References

  1. Ambrose, W. P. et al. Chem. Rev. 99, 2929–2956 (1999).

    Article  CAS  Google Scholar 

  2. Shera, E. B., Seitzinger, N. K., Davis, L. M., Keller, R. A. & Soper, S. A. Chem. Phys. Lett. 174, 553–557 (1990).

    Article  CAS  Google Scholar 

  3. Schmidt, T., Schütz, G. J., Baumgartner, W., Gruber, H. J. & Schindler, H. Proc. Natl Acad. Sci. USA 93, 2926–2929 (1996).

    Article  CAS  Google Scholar 

  4. Tokunaga, M., Kitamura, K., Saito, K., Iwane, A. H. & Yanagida, T. Biochem. Biophys. Res. Commun. 235, 47–53 (1997).

    Article  CAS  Google Scholar 

  5. Yanagida, T. & Ishii, Y. (eds) Single Molecule Dynamics in Life Science (John Wiley & Sons, 2008).

  6. Rust, M. J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–796 (2006).

    CAS  Google Scholar 

  7. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  CAS  Google Scholar 

  8. Shechtman, Y., Weiss, L. E., Backer, A. S., Sahl, S. J. & Moerner, W. E. Nano Letters 15, 4194–4199 (2015).

    Article  CAS  Google Scholar 

  9. Weiss, L. E. et al. Nat. Nanotechnol https://doi.org/10.1038/s41565-020-0662-0 (2020).

    Article  Google Scholar 

  10. Castello, M. et al. Nat. Methods 16, 175–178 (2019).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Jörg Enderlein.

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Enderlein, J. Single-molecule imaging goes high throughput. Nat. Nanotechnol. 15, 419–420 (2020). https://doi.org/10.1038/s41565-020-0676-7

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