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Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry


Several Ebola viruses cause outbreaks of lethal haemorrhagic fever in humans, but developing therapies tackle only Zaire Ebola virus. Dendritic cells (DCs) are targets of this infection in vivo. Here, we found that Ebola virus entry into activated DCs requires the sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169), which recognizes sialylated gangliosides anchored to viral membranes. Blockage of the Siglec-1 receptor by anti-Siglec-1 monoclonal antibodies halted Ebola viral uptake and cytoplasmic entry, offering cross-protection against other ganglioside-containing viruses such as human immunodeficiency virus type 1.

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Fig. 1: Activation of DCs enhances Ebola viral binding and uptake via Siglec-1 recognition of gangliosides.
Fig. 2: Siglec-1 facilitates the uptake of Ebola viruses displaying envelope glycoproteins in VCCs.
Fig. 3: Ebola virus uptake by Siglec-1 facilitates cytoplasmic viral entry into activated DCs.
Fig. 4: Generation and characterization of anti-Siglec-1 mAbs.
Fig. 5: Anti-Siglec-1 mAbs inhibit HIV-1 and Ebola viral uptake.
Fig. 6: Anti-Siglec-1 mAbs block HIV-1 trans-infection and cytoplasmic Ebola entry.

Data availability

The data that support the findings of this study are available from the corresponding authors upon reasonable request.


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For their excellent assistance, advice and imaging processing we thank E. Rebollo and J. Boix from the Advanced Fluorescence Microscopy Unit IBMB-PCB. We are also grateful to A. M. García-Cabrero and M. Lozano from Protein Tools at CNB for mAb purification. At HUGTiP, we thank all surgeons from the Otorhinolaryngology Department for their support, and E. Sayós Ortega and L. Pérez-Roca from the Histopathology & Electron Microscopy Platform for their outstanding sample processing and management. We thank P. Resa-Infante for critical reading of our manuscript. We thank C. Muñoz-Fontela for helpful discussions. We thank C. Galvez for PCR identification of the Siglec-1 null individual. We also thank the Microscopy Core Facility at IGTP for providing access to the Zeiss 710 confocal microscope, and the Advanced Light Microscopy Unit at the Centre for Genomic Regulation (CRG, Barcelona, Spain) for access to the Leica STED microscope. J.M.-P. and N.I.-U. are supported by the Spanish Secretariat of State of Research, Development and Innovation through grant No. SAF2016-80033-R. J.M.-P. is supported by the Spanish AIDS network Red Temática Cooperativa de Investigación en SIDA. D.P.-Z. is supported by the Spanish Ministry of Science, Innovation and Universities and the European Regional Development Fund under agreement No. BES-2014-069931. J.C. is supported by Catalan Department of Health, PERIS fellowship (No. SLT006/17/00214). S.B. is supported by the Rio Hortega programme funded by the Spanish Health Institute Carlos III (No. CM17/00242). X.M.-T. is supported by the Spanish Ministry of Science, Innovation and Universities and the European Regional Development Fund under agreement No. BES-2017-082900. This research was sponsored in part by Grifols.

Author information




D.P.-Z., L.K., J.M.-P. and N.I.-U. conceived and designed the experiments. M.G.-G., M.M. and L.K. generated and characterized the mAbs. D.G. contributed materials. D.P-Z, I.E., M.P., M.G.-G., M.M., S.B., J.C. and X.M.-T. performed the experiments. D.P.-Z., I.E., M.P., M.G.-G., M.M., S.B., J.C., M.F.F., V.U., L.K., J.M.-P. and N.I.-U. analysed and interpreted the data. D.P.-Z. and N.I.-U. wrote the paper. J.M.-P. and N.I.-U. performed critical revision.

Corresponding authors

Correspondence to Javier Martinez-Picado or Nuria Izquierdo-Useros.

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A patent application based on this work has been filed (US 62/828,195). The authors declare no other competing interests.

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Supplementary information

Supplementary Information

Supplementary Tables 1–4, Supplementary Figures 1–10 and Supplementary Video Legends.

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Supplementary Video 1

Confocal microscopy analysis of LPS DCs. Cells were pulsed and labeled as in Fig. 2h. Videos show 3D reconstruction of the maximum intensity fluorescence built with Volocity Software.

Supplementary Video 2

Confocal microscopy analysis of LPS DCs. Cells were pulsed and labeled as in Fig. 2h. Videos show 3D reconstruction of the maximum intensity fluorescence built with Volocity Software.

Supplementary Video 3

Super-resolution microscopy analysis of the VCC from LPS DCs. Cells were pulsed and stained as in Fig. 2j. Videos show 3D reconstruction built with Imaris Software.

Supplementary Video 4

Super-resolution microscopy analysis of the VCC from LPS DCs. Cells were pulsed and stained as in Fig. 2j. Videos show 3D reconstruction built with Imaris Software.

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Perez-Zsolt, D., Erkizia, I., Pino, M. et al. Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry. Nat Microbiol 4, 1558–1570 (2019).

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