Cigarette smoking is a risk factor for a wide range of human diseases1. To investigate the genetic components associated with smoking behaviours in the Japanese population, we conducted a genome-wide association study of four smoking-related traits using up to 165,436 individuals. In total, we identified seven new loci, including three loci associated with the number of cigarettes per day (EPHX2–CLU, RET and CUX2–ALDH2), three loci associated with smoking initiation (DLC1, CXCL12–TMEM72-AS1 and GALR1–SALL3) and LINC01793–MIR4432HG, associated with the age of smoking initiation. Of these, three loci (LINC01793–MIR4432HG, CXCL12–TMEM72-AS1 and GALR1–SALL3) were found by conducting an additional sex-stratified genome-wide association study. This additional analysis showed heterogeneity of effects between sexes. The cross-sex linkage disequilibrium score regression2,3 analysis also indicated that the genetic component of smoking initiation was significantly different between the sexes. Cross-trait linkage disequilibrium score regression analysis and trait-relevant tissue analysis showed that the number of cigarettes per day has a specific genetic background distinct from those of the other three smoking behaviours. We also report 11 diseases that share genetic basis with smoking behaviours. Although the current study should be carefully considered owing to the lack of replication samples, our findings characterized the genetic architecture of smoking behaviours. Further studies in East Asian populations are warranted to confirm our findings.
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GWAS summary statistics of the smoking behaviours are publicly available at our website (JENGER; http://jenger.riken.jp/en/) and the National Bioscience Database Center (NBDC) Human Database (research ID: hum0014) as open data without any access restrictions. The accession numbers for each phenotype in the NBDC Human Database are as follows: age of smoking initiation: hum0014.v14.asi.v1; CPD: hum0014.v14.cpd.v1; smoking initiation: hum0014.v14.ens.v1; smoking cessation: hum0014.v14.fcs.v1. GWAS genotype data from the participants were deposited at the NBDC Human Database (research ID: hum0014).
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We acknowledge all patients who participated in the study. We thank the staff of the BBJ for their collecting and managing of clinical information and samples. We also thank the contributions of the Tohoku Medical Megabank Project, the Japan Public Health Center-based Prospective (JPHC) Study, the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study, and the Genetic Study Group of Investigation Committee on Ossification of the Spinal Ligaments for the case–control studies used in this study. This research was supported by the Tailor-Made Medical Treatment Program (the BioBank Japan Project) of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), and the Japan Agency for Medical Research and Development (AMED) (grant ID: JP17km0305002). N.I. and M.I. were funded by the AMED under JP18dm0107097, JP18km0405201 and JP18km0405028. S.I. was funded by the AMED under JP18ek0109223 and JP18bm0804006. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
The authors declare no competing interests.
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Matoba, N., Akiyama, M., Ishigaki, K. et al. GWAS of smoking behaviour in 165,436 Japanese people reveals seven new loci and shared genetic architecture. Nat Hum Behav 3, 471–477 (2019). https://doi.org/10.1038/s41562-019-0557-y
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