Abstract
Understanding how and why local communities change is a pressing task for conservation, especially in freshwater systems. It remains challenging because of the complexity of biodiversity changes, driven by the spatio-temporal heterogeneity of human pressures. Using a compilation of riverine fish community time series (93% between 1993 and 2019) across the Palaearctic, Nearctic and Australasia realms, we assessed how past and recent anthropogenic pressures drive community changes across both space and time. We found evidence of rapid changes in community composition of 30% per decade characterized by important changes in the dominant species, together with a 13% increase in total abundance per decade and a 7% increase in species richness per decade. The spatial heterogeneity in these trends could be traced back to the strength and timing of anthropogenic pressures and was mainly mediated by non-native species introductions. Specifically, we demonstrate that the negative effects of anthropogenic pressures on species richness and total abundance were compensated over time by the establishment of non-native species, a pattern consistent with previously reported biotic homogenization at the global scale. Overall, our study suggests that accounting for the complexity of community changes and its drivers is a crucial step to reach global conservation goals.
Similar content being viewed by others
Main
Biological communities are undergoing dramatic reassembly in response to an array of ever-growing human impacts1. Changes in species composition and not necessarily systematic reductions in local-scale species richness are becoming increasingly recognized2,3, often resulting in ecosystem consequences manifested across large spatial scales4,5. Repeated calls have been made for greater scientific clarity regarding how heterogeneous rates of species losses and gains across space may shift community structure over time6. Advancing this knowledge is particularly relevant for freshwater ecosystems, where vertebrate populations are declining substantially faster than those in terrestrial or marine systems7.
Land use conversion is a persistent and pervasive threat to freshwater ecosystems8 with striking repercussions for freshwater fish biodiversity9,10. Dense urban and cultivated areas are often associated with reduced species richness and abundance11,12, and shifts in local community composition towards more tolerant and ubiquitous species that can cope with degraded conditions13,14. Non-native species can also play a disproportionate role in the reassembly of communities over time5,15,16,17, and have dramatic effects on native species when they become invasive18, including the widespread homogenization of faunas19. Hubs of human activities such as human settlements, transport and trade are also responsible for major habitat alterations and increased accessibility, resulting in more frequent non-native introduction events and opportunities for spread20,21,22. Human activities may therefore have opposing effects on local diversity by decreasing the number and abundance of native species, while concurrently promoting the establishment and spread of non-native species that can increase community total abundance and species richness4,6. Understanding community changes therefore requires going beyond analyses of changes in the number of species or individuals by considering concomitant changes in species identity2,3,23,24.
Temporal changes in community composition are influenced by past anthropogenic pressures that can generate transient ecological dynamics and long-lasting biotic ‘legacies’25. Given the high spatio-temporal heterogeneity of anthropogenic pressures26, ignoring the long-term antecedent effects of historical pressures and their recent changes can greatly impede our understanding of the drivers of community change, such as what has been demonstrated for the effects of invasive species27. Additionally, habitat structure and connectivity can enhance or dampen community responses to anthropogenic pressures by mediating dispersal among habitats28. Accounting for past and recent anthropogenic pressures as well as spatial distribution of habitats may therefore improve our understanding of community changes.
This study investigates the spatio-temporal changes of riverine fish communities in response to human pressures from local to continental extents. To do so, we leveraged a compilation of 4,476 riverine fish community time series29 that had been repeatedly sampled from 1957 to 2019 using variable durations and frequencies (93% of the samplings between 1993 and 2019 with a minimum of 5 years of sampling; Extended Data Fig. 1), mainly using electrofishing (98% of the samplings). The sites are located in various river basins, mainly across the Palaearctic, Nearctic and Australasia realms (99.9% of the sites). We used Bayesian hierarchical models to assess temporal changes in total abundance, species richness and community composition across local communities, including in the share of non-native species. We next characterized the typology of community temporal trends by examining the covariations among different community metrics, and identifying trajectories of community change across spatial scales. We finally assessed how fish community changes could be traced back to the spatio-temporal changes in anthropogenic pressures and longitudinal stream position. Anthropogenic pressures were quantified with the human footprint index, which includes an array of pressures such as population density, land use and human-built infrastructure30 (Methods), and has been previously related to species extinction and invasion risks31,32. Outcomes of this study further our understanding of the complexity of local community changes by addressing the effects of global change and advancing new knowledge that can inform actions seeking to curb the current freshwater biodiversity crisis.
Results
Community temporal trends
Riverine fish communities have demonstrated remarkable change over recent decades (Fig. 1; range first survey year: 1957–2010, median = 1997; time span: 10–60 years, median = 17; see Extended Data Fig. 1 for more details on the time series). We estimated temporal trends with a hierarchical Bayesian modelling approach that accounts for spatial variation at both hydrographic river basin and site levels (that is, by including random terms on the intercept and temporal trends; see Methods for a detailed description of the models), finding that communities have increased in both total abundance and species richness, but decreased in the proportional abundance of non-native species. We further found that the estimated temporal trends were not influenced by the characteristics of the time series, such as the temporal span, survey completeness and starting year (Extended Data Fig. 2). From this model, we considered weak, medium and strong evidence for an effect when its credible interval at respectively 80, 90 and 95% did not overlap zero33,34. We found strong evidence for an average increase in total community abundance (average credible interval (CI) 95%: 13.2% (2.9%, 23.8%) per decade; Fig. 1a) and in species richness (CI 95%: 6.9% (3.9%, 9.9%) per decade; Fig. 1b) over time. By contrast, we found an average decline in the proportion of non-native species abundance (moderate evidence, CI 90%: −0.0047 (−0.0091, −0.0004) per decade; Fig. 1c), and no evidence for a temporal trend in the proportion of non-native species richness (CI 80%: 0.001 (−0.001, 0.004) per decade; Fig. 1d).
Changes in abundance and species richness were accompanied by rapid compositional reorganization, with an average decline in community similarity of about 30% per decade when considering either species abundances (Simpson dissimilarity, CI 95%: 0.33 (0.31, 0.34) per decade, hereafter ‘temporal dissimilarity’; Fig. 1e) or occurrences (Jaccard dissimilarity, CI 95%: 0.31 (0.30, 0.33) per decade; Extended Data Fig. 3a). The consistency in the Simpson and Jaccard dissimilarity metrics indicated that changes in temporal dissimilarity resulted from changes in the identity of the dominant rather than of the rare species. The partitioning of the Jaccard dissimilarity index into turnover, describing composition changes arising from species replacement, and nestedness, describing changes arising from species gains or losses from a common species pool, further showed a comparable increase over time (CI 95%: 0.17 (0.16, 0.18) and 0.16 (0.15, 0.17) per decade respectively; Fig. 1f and Extended Data Fig. 3b). This suggests that changes in community composition were driven by species replacement in the community, in addition to species losses or gains.
Beyond overall temporal trends, considerable spatial heterogeneity exists across sites, as illustrated by the spread of the histograms in Fig. 1. This heterogeneity is also apparent within the same river basin (Supplementary Software 1). For example, on average the Thames basin shows the same spatial patterns as at the global scale, but a variety of temporal trends were observed across the 139 sites within the basin, including decrease in species richness (18 sites), decrease in abundance (20) and high turnover (17). The (random) slope of time in our hierarchical models varied much more (that is, up to more than twice as much) across sites within river basins than across different basins for all community metrics (Extended Data Table 1). This suggested that relatively finer-scale environmental variation within river basins has a greater effect on community changes than larger-scale environmental or biogeographical variation across river basins.
Typology of community temporal trends
We further assessed covariations among the temporal trends of different community metrics to identify potential ‘types’ of community temporal trajectory, using the temporal trends at the site level estimated from the hierarchical Bayesian model. There was a moderate level of association among the different community trajectory metrics; the first two axes of the principal component analysis (PCA) explained 69% of the total variability among fish communities (Fig. 2a–b). Temporal trends in community composition (that is, temporal dissimilarity and turnover) were positively associated with each other, as were temporal trends in total abundance and species richness; however, these two sets of trajectories appeared largely independent of each other (Fig. 2a). Using a k-mean trimmed clustering method on the temporal trends in the community metrics at the site level (Methods), we further detected six distinct types of community trajectory (Fig. 2c; non-assigned sites are displayed in Extended Data Fig. 4). The largest cluster was characterized by moderate changes along all biodiversity dimensions: medium temporal increases in total abundance and species richness, temporal dissimilarity, and turnover (‘medium change’; 42% of the sites). The second cluster was associated with communities showing strong turnover but moderate increases in total abundance, species richness and temporal dissimilarity (‘high turnover’; 16% of the sites). The third, fourth and fifth clusters were characterized by temporal community changes along a single dimension: a strong increase in species richness (‘increase in species richness’; 13% of the sites), a strong decline in total abundance (‘decrease in total abundance’; 12% of the sites) or a strong decline in species richness (‘decrease in species richness’; 9% of the sites), respectively. The last and smallest cluster was associated with communities that remained relatively stable over time (‘low change’; 7% of the sites). The relative frequency of the different community trajectories was broadly similar across the three main biogeographic realms (Fig. 2d).
Drivers of community temporal trends
We detected complex synergies between the legacy of past anthropogenic pressures and the effects of recent anthropogenic pressures on community temporal trends, by considering additional predictors associated with the human footprint index and longitudinal stream position (Fig. 3a; see model predictions in Extended Data Fig. 5). In addition, we found that these additional predictors were not related to the characteristics of the time series (Extended Data Fig. 6). Specifically, we found strong evidence that a higher degree of past anthropogenic pressures (that is, human footprint index of 1993 corresponding to the beginning of the time series) was associated with faster increases in total abundance and species richness (respective CI 95%: 0.02 (0.01, 0.04) in blue and 0.03 (0.01, 0.05) in green; Fig. 3a). We also uncovered evidence for an interaction with the longitudinal stream position (that is, represented by a synthetic PCA axis based on several hydromorphological characteristics where high values are associated with more downstream areas; Extended Data Fig. 7), such as the legacy effects of past anthropogenic pressures on total abundance (strong evidence) and species richness (weak evidence) were buffered in more downstream areas (respective CI 95%:\({\beta }^{{\prime} }=\)−0.021 (−0.031, −0.011) and CI 80%: −0.0108 (−0.0191, −0.0025); Fig. 3a). Similar results were obtained using raw or coverage-based species richness (Methods and Extended Data Fig. 8).
Past anthropogenic pressures were also associated with changes in community composition. We found evidence (albeit weak) that a higher degree of past anthropogenic pressures was associated with an increase in the proportion of non-native richness over time (CI 80%: 0.02 (0.00, 0.04) in orange; Fig. 3a), and that this effect was enhanced in more downstream areas (CI 90%: 0.023 (0.003, 0.043); Fig. 3a). Although we found no overall associations between past anthropogenic pressures and temporal trends in non-native species abundance (CI 80%: 0.00 (−0.02, 0.03) in yellow; Fig. 3a), we found moderate evidence that a higher degree of past anthropogenic pressures resulted in a higher rate of increase in non-native species abundance in the most downstream areas (CI 90%: 0.033 (0.003, 0.064); Fig. 3a). We also found that a higher degree of past anthropogenic pressures was associated with faster rates of increases in temporal dissimilarity and turnover (respective CI 95%: 0.23 (0.20, 0.27) in red and 0.25 (0.21, 0.29) in purple; Fig. 3a), irrespective of the longitudinal stream position (respective CI 80%: −0.017 (−0.036, 0.002) and −0.016 (−0.037, 0.004); Fig. 3a). This result was consistent between Jaccard and Simpson-based dissimilarity (Extended Data Fig. 8), but the effects of past anthropogenic pressures were attenuated in most downstream areas for Jaccard’s dissimilarity, indicating that those changes involved more dominant species (Extended Data Fig. 8).
Recent increases in anthropogenic pressures (that is, ratio of the human footprint index between 2009 and 1993) were found to have a context-specific effect on total abundance and species richness, as well as to hasten community reorganization through increases in the share of non-native species and faster rates of temporal dissimilarity and turnover (Fig. 3a; see model predictions in Extended Data Fig. 5). More specifically, we found strong evidence of an antagonistic effect between past and recent anthropogenic pressures on total abundance (CI 95%: −0.018 (−0.034, −0.002); Fig. 3a), such as the rate of increase observed across the historically most degraded sites was lower when these sites experienced a recent increase in anthropogenic pressures, although recent anthropogenic pressures per se had no effect on the temporal trends in total abundance (CI 80%: −0.004 (−0.010, 0.002); Fig. 3a). Similarly, recent changes in anthropogenic pressures did not have an overall effect on the temporal trends in species richness (CI 80%: −0.005 (−0.012, 0.003); Fig. 3a), but we found moderate evidence for a positive interaction with the longitudinal stream position (CI 90%: 0.0063 (0.0004, 0.0121); Fig. 3a), indicating that recent increases in anthropogenic pressures were associated with faster increases in species richness over time in more downstream areas.
Recent increases in anthropogenic pressures were also associated with more rapid increases in the proportion of non-native species abundance in more downstream areas (CI 90%: 0.015 (0.002, 0.029); Fig. 3a), although recent changes in anthropogenic pressures had no overall effect (CI 80%: −0.004 (−0.010, 0.002); Fig. 3a). When considering the share of non-native species richness, we found moderate evidence that a recent increase in anthropogenic pressures was associated with an increase in the proportion of non-native species (CI 90%: 0.02 (0.00, 0.04); Fig. 3a). This effect was particularly pronounced in the historically most degraded sites and in the most downstream areas (respective CI 90%: 0.032 (0.000, 0.063) and 0.010 (0.001, 0.019); Fig. 3a). In addition, we found strong evidence that a recent increase in anthropogenic pressures resulted in stronger temporal dissimilarity and turnover (respective CI 95%: 0.05 (0.03, 0.08) and 0.08 (0.06, 0.11); Fig. 3a). These effects were hastened in the most historically degraded sites (respective CI 95%: 0.06 (0.01, 0.10) and 0.11 (0.06, 0.16); Fig. 3a), but not affected by the longitudinal stream position (respective CI 80%: 0.00 (−0.01, 0.01) and −0.01 (−0.02, 0.01)). By contrast, our results indicated that an increase in recent anthropogenic pressures in the historically most degraded sites was associated with slower rates of increase in total abundance and species richness, but faster rates of increase in non-native richness, temporal dissimilarity and turnover (see model predictions in Extended Data Fig. 5).
Drivers of community variation across space
Spatial variation in community structure was strongly associated with past and recent anthropogenic pressures and with longitudinal stream position (that is, single model effects independent of time; Fig. 3b). Using baseline model prediction (that is, at t = 0; Extended Data Fig. 5 and Methods), we found that a higher degree of past anthropogenic pressures was associated with lower total abundance (strong evidence; Fig. 3b, blue), with the most ‘degraded’ sites (that is, with a human footprint index = 45.6) displaying a total abundance 30% lower than the most ‘intact’ sites (that is, with a human footprint index = 2.5). By contrast, a higher degree of past anthropogenic pressures was associated with higher species richness (strong evidence; Fig. 3b, green), with the most degraded sites displaying 64% more species than the most intact sites.
Recent increases in anthropogenic pressures were strongly associated with lower total abundance and species richness (Fig. 3b). More specifically, sites that experienced a twofold increase in recent anthropogenic pressures had 16% lower total abundance and 7% lower species richness than sites that had not undergone such pressures. Longitudinal stream position was strongly associated with species richness—the most downstream sites displayed three times more species than the most upstream sites (Fig. 3b).
Anthropogenic pressures and longitudinal stream position were associated with spatial variation in the proportion of non-native fish species. Sites that had experienced a higher degree of past and recent anthropogenic pressures had a higher proportion of non-native individuals and species (strong evidence; Fig. 3b, yellow and orange). The proportion of non-native individuals and species was three times (9% versus 3%) and two times (10% versus 4%) higher in the most degraded sites than the most intact sites. Further, a twofold increase in recent anthropogenic pressures was associated with an increase in the proportion of non-native individuals and species by 69% and 63%, respectively. The most downstream sites had 33% higher proportion of non-native abundance and 78% higher proportion of non-native richness than the most upstream sites (Fig. 3b). Noteworthy, a larger share of the variance in the community metrics was explained by site and basin identity rather than by the fixed effects alone (R2 conditional varying from 0.15 for turnover to 0.80 for species richness versus R2 marginal varying from 0.02 for species richness to 0.07 for community turnover; Table 1). This indicates that context dependencies are well captured by our hierarchical models but suggests that integrating fine-scale local drivers may further improve our ability to predict local community changes.
Discussion
Recent decades have witnessed substantial shifts in riverine fish communities characterized by marked increases in species richness and total abundance over time, accompanied by a strong pattern of species replacement. We found that fish species richness has increased at a rate of ~7% per decade, although no net change in species richness had been previously reported in terrestrial and in marine systems2,3,35. We also found an overall increase in total fish abundance of ~13% per decade, which is in line with the increase of 11% per decade reported for freshwater insects33. This is also consistent with several regional assessments of freshwater population trends in the Palaearctic, such as the reported increase in freshwater insect occupancy documented in the UK or the increase in freshwater animal Living Planet Index in the Netherlands since the 1990s36,37. However, this finding contrasts with dramatic Living Planet Index declines reported at the global scale for freshwater species and particularly fish megafauna, as well as with other regional assessments of fish assemblages8,38. We further found a faster average temporal trend in Jaccard dissimilarity (31% versus 10% per decade) but a slower average turnover (17% versus 28% per decade) than previously reported across a diversity of marine, freshwater and terrestrial assemblages2,3, indicating that riverine fish communities experienced both important richness and compositional changes in recent decades.
These recent biotic changes are linked to complex spatio-temporal processes involving past and recent human impacts on the environment and their interaction with stream network position. Higher past anthropogenic pressures were associated with faster rates of species richness and total abundance increases over time, suggesting a recovery from the legacy of past disturbances. Previous studies suggested that the adoption of numerous legislations targeting improvements in water quality in the European Union and the United States since the 1970s, as well as a decrease in the negative effects of agriculture, could be partly responsible for those increases despite the surrounding habitat changes33,39,40. The fact that most of the study sites (92%) were already highly degraded at the beginning of the study period, that is, they had a human footprint index > 4 in 199341, could lend support to the recovery hypothesis.
However, a higher degree of past anthropogenic pressures was also associated with a higher share of non-native species; this effect being stronger in downstream sites. This indicates that the introduction and establishment of non-native species contributed most substantively to the fish community changes through time in the sites that suffered the greatest past (pre-1993) degradation, and particularly the most downstream ones. The increase in local species richness over time in degraded rivers could thus result from introduction of non-native species from ongoing spatial homogenization17, a pattern well-supported by metacommunity models42 and already documented across river basins in the Nearctic and Palaearctic realms17. This is in line with findings that higher densities of human population, urban areas and roads—all included in the human footprint index—can promote non-native species richness by increasing the number and frequency of introduction events15,16,31. Anthropogenic pressures can also alter the instream habitat to be more conducive for non-native species that are often ubiquitous and habitat generalists13,14, giving them a competitive advantage over native species that are less suited to the new conditions16,43,44,45. A higher degree of past anthropogenic pressures was also associated with faster rates of species replacement and shift in species dominance over time. This suggests that the legacy effects of past habitat degradation are characterized by shifts towards species that are better adapted to degraded environments9, to which non-native species contribute disproportionately2,3.
This study uncovered important interaction effects between past and recent human pressures in driving the rate of change in several community metrics, highlighting the importance of considering both the degree and timing of anthropogenic pressures. For example, as discussed above, communities that experienced greater past degradation had actually experienced an increase in richness in recent years. But an increase in recent human pressures at these sites was associated with an increase of non-native species and lower species richness. This suggests that any recovery of the native fish communities in previously degraded sites would be severely compromised if human impacts were allowed to continue. Conversely, these findings clearly illustrate opportunities to reduce human impacts in previously degraded habitats to benefit freshwater biodiversity7. In turn, the fact that non-native species were more abundant in both historically or recently degraded sites, but that no direct association was uncovered in terms of non-native temporal trends, can be explained, at least in part, by commonly reported time lags between the first recorded introductions and the establishment of self-sustaining populations, which includes time for biological acceptance and local adaptation16. Our results demonstrate that recent habitat degradation can result in simultaneous negative and positive effects on native and non-native species, respectively6,46, and highlights the conservation challenges associated with the identification and management of biodiversity changes in the context of transient community dynamics25.
Longitudinal position along the river network was found to mediate temporal biodiversity trends, with the most downstream sites experiencing faster rates of community change over time. This finding may be explained by the higher connectivity of larger rivers with other tributaries, which in turn gives more opportunity for local colonization28 and the establishment of metacommunity dynamics47. As such, it is not entirely surprising that community changes were found to be more heterogeneous at the local scale than at the basin or realm scales, and that the spatial structure of the model explained much more of the variance in the community metrics than the fixed effects. This probably reflects the characteristics of riverine habitats, and especially their dendritic structure and isolation within drainage basins, which determine environmental filtering and dispersal opportunities48,49. In addition, we focused chiefly on community reorganization arising from land use pressures, therefore disregarding the potential interactions with other global drivers of change such as climate change and more localized threats such as water withdrawals40,50.
Our results further confirm that temporal changes in species composition can be decoupled from changes in species richness in freshwater systems, similarly to what has been observed in mostly marine and terrestrial assemblages2,3. Various community trajectories can be linked to a complex mosaic of ecological drivers such as the degree and timing of anthropogenic pressures and position of the sites within watersheds. It follows that the similar frequencies in community trajectories detected across realms, together with the restricted number of sites displaying a low degree of change, probably reflects the spatially and temporally heterogeneous patterns in human pressures. We recognize that recent increases in anthropogenic pressures are most prevalent in tropical biodiversity hotspots30,51, while our study has a spatial coverage limited to historically industrialized countries and in mostly temperate biomes. Noticeably, anthropogenic pressures decreased by 4% on average (based on differences in the human footprint index between 1993 and 2009) across the sites included in our study, while it increased by 25% across the rivers globally52. Consequently, species richness and abundance increases as well as the decoupling of compositional changes from richness changes may not be universal phenomena, and more heterogeneous patterns of biotic change may manifest at the global scale once we consider tropical fish communities. Our study remains essentially correlative. Although aggregated anthropogenic threat indices have been shown to be useful to estimate the ecological integrity of freshwater ecosystems53, they do not replace the use of more targeted threat indices related to water quality and ecosystem functioning54,55. Nonetheless, our analyses were conducted on the best available temporal riverine fish dataset at this time, and they provide evidence linking the multidimensionality of community changes to the interplay between past and recent environmental challenges as well as habitat context. Our study also offers a framework for future research that merges multiple scales of both time and space4,6, which could be leveraged as more tropical data are collected and become available.
In conclusion, our study uncovered complex but consistent effects of past and recent changes in anthropogenic pressures and stream network position on riverine fish communities. We showed that the timing of anthropogenic pressures matters, because past and recent pressures can have contrasting and interactive effects on community trends, partly mediated by non-native species. Our study further shows that considering multiple biodiversity facets can shed light on the complex mechanisms by which communities change over time. Looking forward, we emphasize the increasing need to investigate biotic changes across spatial scales to better reconcile reported local gains and global declines in biodiversity4,6,56. The increasing availability of community time series and environmental data across large areas is invaluable for understanding how human pressures impact biodiversity across taxa and ecosystems, and for implementing conservation policies to mitigate these impacts.
Methods
Fish community time series
We used the RivFishTIME database29, a compilation of more than 12,000 time series containing species abundances of riverine fish communities, which we completed with time series from Canada and the United States (Supplementary Table 2). The final database mainly covered western and northern Europe, northern America, and southeastern Australia. We selected time series having at least 5 years of data over a 10-year period as well as a consistent sampling protocol and abundance unit. As several sites had been sampled using different sampling methods (for example, electrofishing, seining; Supplementary Table 2), we selected for each site only the sampling events that were performed using the most frequent sampling method. To minimize the influence of seasonal variation in community composition (for example, due to spawning or migration), we further only selected sampling events that were performed within 1.5 months of the most frequently sampled month (that is, 45 days before or after). When there were several sampling events in a given year, we selected the one that took place at the closest date from the most frequently sampled date of the site. Finally, we excluded 1,340 sites that had been limed as part of the long-term Swedish liming programme (https://kalkdatabasen.lansstyrelsen.se/)57 to avoid including sites whose environmental conditions had been experimentally manipulated. The data selection resulted in 4,476 fish community time series, totalling 46,932 sampling events, 326,717 species abundance records and 806 freshwater fish species. The median time span of the time series was of 17 (13, 23; 25th quantile, 75th quantile) years, the median completeness (that is, number of annual samplings/time span) was of 55% (38%, 78%) (Extended Data Fig. 1) and the median first study year was 1997 (1992, 2003). The sites were mostly located in the Palaearctic (75%), Nearctic (20%) and Australasia (5%), and distributed across 307 hydrographic basins. Four countries gathered 85% of the sites, namely Great Britain (29%), France (21%), Sweden (18%) and the United States (18%; Supplementary Table 2).
Community metrics
We assessed community changes in riverine fish communities using several biodiversity facets related to total abundance, species richness, the share of non-native species and community composition (Supplementary Table 2). Total abundance was reported in number of individuals (47.00% of the sampling events), density of individuals per 100 m2 (51.81%), catch per unit effort (CPUE; 1.05%) and Leslie index (0.14%; Supplementary Table 2). Although we selected for strict protocol consistency, 70% or more of the sampling events by unit of abundance did not report sampling effort, preventing us from harmonizing count, abundance density and CPUE29.
As sampled species richness is a negatively biased estimator of the ‘true’ species richness, we corrected sampled species richness with the coverage-based rarefaction and extrapolation methodology58. The estimated coverage of a sample is positively related to the number of individuals and negatively related to the number of singletons. We fixed the coverage of all samples at 98.5% via rarefaction and extrapolation using the R package iNEXT59 to make species richness comparable across samples. We did not always have a direct estimate of the number of individuals and number of singletons to compute the sampling coverage, as 51.81% of the abundances were measured as density values and 1.05% as CPUE. In this case, we first divided each species density (xi) by the minimum value in the community and rounded each value to the nearest integer (that is, \({x}_{i}^{{\prime} }={\rm{round}}(1/{\rm{min}}({x}_{i}))\), where \({x}_{i}^{{\prime} }\) is the estimated abundance of the ith species) to obtain at least one singleton species, that is, a species with one individual. However, we note that both covered-based and raw species richness estimates were highly correlated (Spearman’s ρ = 0.97 for both raw variables and log-transformed ones), and the choice of the metric did not influence our interpretations regarding the patterns and drivers of species richness changes (Extended Data Fig. 7).
The biogeographic origin of the fish species describing whether species were native or introduced to a given drainage basin was retrieved using a global database documenting species status across drainage basins of the world60 (94.3% of the species occurrences; Supplementary Table 2). For the sites falling outside the river basins provided in the global database, such as for the sites located close to the shore, we used the closest basin within the same country. For species not included in a given drainage basin, we determined the origin of the species at the country scale using FishBase61 (5.5% of species occurrences). Given the spatial extent of the United States, we completed the global database with the Nonindigenous Aquatic Species database developed by the US Geological Survey (https://nas.er.usgs.gov/) using US states as spatial grain (0.05% of the species occurrences). We completed the remaining species origins at the country scale, using national atlases and FishBase data in neighbouring countries, such as for Piaractus brachypomus and Rutilus rutilus in the United States (0.1% of the species occurrences; Supplementary Table 2). We then estimated the percentage of non-native species with respect to both abundance and species richness for each sampling event (Supplementary Table 3).
Dissimilarity metrics
We used the complement of the Jaccard similarity index (that is, Jaccard dissimilarity, which we denote as J) to characterize temporal dissimilarity in community composition at each site, taking the first year of sampling of a community as the reference community. This index is based on presence/absence and is simply the sum of species gains and losses over the total number of species across two samples (equation (1)). It thus measures the proportion of species not shared between two samples.
with Sgain, Sloss and Stot being the numbers of immigrant, extirpated and total species, respectively.
We further partitioned the Jaccard dissimilarity index into two complementary indices, turnover (Jt) and nestedness (Jn), respectively Jt = (2 × min(Sloss, Sgain))/(Scommon + (2 × min(Sloss, Sgain))) and Jn = 1 − Jt, Scommon being the number of species present in both communities62. High turnover values indicate that the changes in community composition result from species replacement, whereas high values of nestedness indicate species gains or losses from a nested community, that is, that a community is a subset of the other63.
We further characterized temporal dissimilarity with the Simpson-based dissimilarity index24 (Hd, equation (2)). This index is based on species relative abundances and their variation across two samples. Simpson-based dissimilarity index is based on the Simpson diversity index and thus gives higher weight to changes in the abundant species, whereas Jaccard dissimilarity index gives equal weight to each species. Simpson-based dissimilarity index thus quantifies the extent of changes in the identity of the dominant species24. Both high Jaccard and Simpson dissimilarity values indicate changes in composition resulting from changes in the abundant species, whereas conjointly high Jaccard and low Simpson dissimilarity values indicate composition changes resulting from changes in the species with low relative abundances.
where i is species i, p is relative abundance and ′ is the focal community.
Environmental drivers
We quantified the degree of anthropogenic pressures using the human footprint index26,30, extracted from the RiverATLAS database at the reach scale52,64 (stream segment length average of 450 m). We did so by snapping the sites to the closest stream segment using a 1 km buffer (99% of the sites). The human footprint index aggregates an array of human pressures, including population density and the extent of urban, forested, cropland and pastureland areas, but also transportation hubs such as roads, railways and navigable pathways. It does so by combining remote sensing data, systematic surveys and modelling from ground data, making it less prone to errors30. The human footprint index ranges from 0 to 50, with values superior to 4 being considered in a degraded state41. This index has been previously related to species extinction and risk of biological invasion32,41. To capture both the effects of the legacy of past anthropogenic pressures and of its recent changes, we considered the human footprint index computed in 1993 and 2009 (that is, a 16-year span). Specifically, the human footprint index of 1993 was used as a measure of past anthropogenic pressures at the beginning of the study period, and the ratio between the human footprint of 2009 and 1993 as a measure of the recent changes in anthropogenic pressures (only 7% of the samplings took place before 1993, while 58% took place between 1993 and 2009, and 34% after 2009; Extended Data Fig. 1). We chose the human footprint index developed in ref. 30 because it goes farther back in time than other related human footprint indices (for example, 1993 versus 2000 in ref. 51). Nevertheless, both indices are highly correlated (Spearman’s ρ = 0.81 for 2009) in overlapping years, suggesting that our results are robust to the choice of human footprint index. In order to obtain interpretable coefficients of recent changes in human footprint, we log-transformed the ratio of human footprint with a base 2, such as values of −1 and 1 represented a division by 2 and a multiplication by 2 of the human footprint between 1993 and 2009, respectively. In river networks, the environmental heterogeneity and connectivity along the longitudinal stream position (upstream–downstream) strongly shape species occurrences, immigration rates and community composition28. To capture this longitudinal gradient, we first described stream characteristics at each site by the altitude (m), slope (°), average annual discharge (m3 s−1), distance from source (km) and Strahler order (that is, downstream position based on stream/tributary hierarchy) that we extracted from the RiverATLAS database at the stream segment scale52,64. We next performed a PCA over the site stream characteristics on the log-transformed (after adding absolute minimum values plus 1 to avoid few negative values in altitude) and standardized variables (that is, centred and scaled). We orthogonally rotated the two first principal components using the varimax criterion65, to increase the quality of the variable representation (that is, their loadings) on the two first principal components. The first rotated component was positively related to average annual discharge, distance from source and Strahler order, and captured 56% of the variance (Extended Data Fig. 8), and was subsequently used as a composite variable describing the longitudinal stream position from upstream to downstream (from negative to positive values, respectively). The correlation coefficients indicated little covariation among the predictors (maximum Spearman’s correlation of 0.09 found between past and recent pressures; Supplementary Fig. 1).
Statistical analysis
To estimate community temporal trends, we first modelled the different community metrics (Y) as dependent of time (β0Timet; equation (3)), measured as the number of years since the beginning of the sampling at each site. The statistical model (equation (3)) was adapted according to the nature of the response variable. For total abundance, we added the measurement unit of abundance as a categorical variable both as a main effect and in interaction with time33. We set the factor level ‘raw count’ as the reference level such that the temporal trends in total abundance in the main text and Supplementary Information are expressed in raw count (that is, number of individuals). For dissimilarity metrics, we set the intercept fixed at 0 as dissimilarity metrics at each site was 0 at the beginning of the time series. We accounted for the hierarchical spatial structure in the data by assigning random effects on the intercept (α) and on the slope of the temporal trends (β0) conditional on basin identity (n) and on site identity (i) nested within basin (i∣n). The random effects and the error terms were modelled as a normal distribution of mean 0 and variance (σ2).
where α = α0 + an + ai∣n, β0 = μ + bn + bi∣n, α0 and μ being the fixed intercept and slope, a and b being the random intercept and slope, εi|n,t the residual error, and \({a}_{n},{a}_{i| n},{b}_{n},{b}_{i| n},{\epsilon }_{i| n,t} \sim {{{\mathcal{N}}}}(0,\,{\sigma }^{2}).\)
To assess the drivers of community change, we then built a second model incorporating additional covariates (Xk, k being the index of the covariate, equation (4)): the degree of past anthropogenic pressures measured by the human footprint index of 1993, the recent changes in anthropogenic pressures measured by the ratio between the human footprint index of 2009 and 1993, and the longitudinal stream position estimated by the rotated PCA axis. We included two-way interactions between time and the ecological drivers (∑k=1β0kTimetXk) to test how longitudinal stream position and anthropogenic pressures affect the temporal trends in community metrics. For instance, a positive interaction between time and past anthropogenic pressures would indicate that faster changes were happening in historically degraded areas. We further included the three-way interactions between time and the pairs of other ecological drivers (∑k=1,k≠lβ0klTimetXkXl, k and l being the indexes of the covariates) to examine the presence of synergistic or antagonistic effects between anthropogenic pressures and longitudinal stream position on the temporal trends in community metrics (that is, the results in Fig. 3a). For instance, a positive interaction between time, recent changes in anthropogenic pressures and longitudinal stream position would indicate that the effects of recent changes in anthropogenic pressures on temporal trends were more important in downstream areas. We included all the predictors as main effects (∑k=1βkXk) to capture the effects of the ecological drivers on the spatial variation in average community composition metrics through time, except when modelling the dissimilarity metrics (that is, we only included the effects of ecological drivers on the temporal trends, the results in Fig. 3b). We did so because dissimilarity metrics quantify community changes at a given time point (t = 0…N) at a given site from the first year (t = 0) and therefore bounded by 0 and 1 at each site; we thus did not expect average differences in dissimilarity related to any other factors than time. We did not include interactions among ecological drivers on the spatial part of the model as we restricted the core of the analysis to the drivers of temporal rather than spatial variation in freshwater fish community composition and tried to keep the model as parsimonious as possible. Finally, we derived the comparison in community metrics across space according to the ecological drivers by using the predictions of the model at the baseline (t = 0). The predictions controlled for the values of other predictors (such as longitudinal stream position and past anthropogenic pressures) by setting them at their median values.
where k, l and ∈ [1, 2, 3] are ecological drivers including stream gradient, legacy of past and recent changes in anthropogenic pressures.
All the response variables were modelled with a Gaussian distribution following previous studies modelling temporal trends in total abundance, species richness and community composition at the global scale2,3,33. Other error structures might be more appropriate to model response variables bounded between 0 and 1 or ratio of discrete numbers such as the dissimilarity metrics and the proportion of non-native species. However, doing so allowed us to obtain easily interpretable coefficients across all community metrics (for example, temporal trends cannot be interpreted as rates of change when modelled using a logit scale such as when using a beta distribution). In addition, a previous study using similar models3 found that slope coefficients estimated with a Gaussian error and a beta error had a Spearman correlation superior to 0.90 and gave qualitatively similar results. We therefore believe that this choice is not likely to alter our conclusions.
We log-transformed the number of years as log(year + 1) as it improved the quality of the model fitting to the data, decreasing the Watanabe–Akaike information criterion66,67 by −733 on average (−11%) across community metrics facets (Supplementary Table 4). It suggested the presence of nonlinearity in the temporal trends, which is particularly expected in the case of bounded variables such as the dissimilarity metrics. We log-transformed total abundance and species richness, so that their temporal trends are multiplicative and can be expressed in percentage change by unit of time. We then derived the percentage of change by decade in species richness and total abundance by back transforming β0 using a time value of log(10 + 1) as follows: \(({\rm{{e}}}^{{\beta }_{0}\times \log (10+1)}-1)\times 100\).
In order to compare the strength of the effects among predictors across community metrics, we scaled both community metrics and the predictors by their standard deviation prior to model fitting. As our models contain interactions, the individual slope coefficients could be difficult to interpret without centring the predictors around ecological relevant values68. As an example, the average temporal trends estimated by β0 in equation (4) can only be interpreted when all the Xk = 0. Hence, we centred past anthropogenic pressures and longitudinal stream position around their average values. The variables quantifying time and recent changes in anthropogenic pressures were not centred, because then the main effects of the ecological predictors (∑k=1βk) would indicate their baseline effects (that is, when time is equal to 0 and without recent changes in anthropogenic pressures).
The models were evaluated in a Bayesian framework using integrated nested Laplacian approximation69,70 (INLA), which approximates the posterior distribution of the parameters and does not rely on Markov chains and Monte Carlo simulations, and thus is a computationally efficient method to evaluate Bayesian models. We computed the CI at 80%, 90% and 95% using the highest posterior density method71, which can respectively be interpreted as weak, moderate and strong evidence of an effect when the interval does not include 0 (refs. 33,34). The statistical models were implemented using the INLA R package69, with defaults uninformative priors. The prior distribution of fixed coefficients followed a flat zero centred normal distribution (\({{{\mathcal{N}}}}(\mu ,\,{\sigma }^{2})={{{\mathcal{N}}}}(0,\,1000)\)). The prior distribution of the random effects and the gaussian error (εit, eq. (3)) followed a log gamma distribution with shape (s) and inverse scale (τ) parameters (\({{{\mathcal{G}}}}(s,\,\tau )={{{\mathcal{G}}}}(1,\,5.1{0}^{-}5)\)). We then back-transformed the estimated coefficients to the standard deviations attributed to the random effects and the gaussian error (\(\sigma =1/\sqrt{\tau }\)). We checked that the slope coefficients, random effects and the temporal trends by basin and site were similar to those obtained with an implementation in frequentist. Then, we concluded that the quality of parameter inference did not suffer from the uninformative priors.
We checked the overall quality of the model fit to the data by plotting the fitted versus observed values (Supplementary Fig. 2). We visually inspected the posterior integral transform and conditional predictive ordinate distribution to assess both the quality of data representation and the frequency of outliers. There was very little multicollinearity in the model, as all variance inflation factors were around 1 (Supplementary Table 5).
We computed marginal (\({R}_{{\rm{m}}}^{2}\)) and conditional (\({R}_{{\rm{c}}}^{2}\)) R2 (equation (5)) to assess the quality of the fit of the Bayesian models, respectively associated with the variance explained by the fixed effects and the variance explained by both the fixed and random effects72. We only included the random effects on the intercept in the R2 computation, that is, the basin effect (an) and the site effect (ai∣n), as the inclusion of the variance attributed to random slopes is much more complex and was shown to not change the results72,73. We computed the variance associated with each predicted value (Varfit) from their posterior distribution, following recommendations to take into account the variability associated to the priors74. We then reported the mean marginal and conditional R2 associated their 95% CI computed using the highest posterior density method.
with yi and \(\hat{{y}_{i}}\) being respectively the observations and the predicted values, and Varfit and Varres being respectively the variance of the predictive means and the variance of the residuals74. an and ai∣n are respectively the standard deviation on the random intercept associated with the hydrographic basin and the site within the basin.
Typology of temporal trends
To estimate the covariations among multiple dimensions of community change, we performed a PCA on the temporal trends in the community metrics estimated at each site with the models (equation (3)) having time as a sole fixed predictor (that is, using the predictions of the models in percentage per decade using the best linear unbiased prediction method). We used four variables in this analysis: temporal trends in total species richness, community abundance, temporal dissimilarity and turnover. We excluded the two variables describing the non-native species composition (proportion of non-native abundance and richness) because their predicted temporal trends at the site level displayed a heavy tailed distribution compared with the other variables (kurtosis of the distributions: 10 versus 21 and 8 versus 15 for total and non-native abundance and species richness, respectively; Fig. 1), which in turn exerted a disproportionate constraint on the analysis. In addition, we performed a clustering analysis on the temporal trends in the community metrics at the site level to identify distinct types of community trajectory using the trimmed k-means method75, a robust clustering method because it avoids the identification of spurious clusters. The method consists of trimming the most outlying data in the multidimensional space, the number of dimensions being the number of community metrics. To choose a relevant number of clusters, we plotted the log-likelihood of the trimmed classification as a function of the proportion of the most outlying data trimmed (α) and the number of clusters (Supplementary Fig. 3). We thus selected a partition of temporal community changes in six clusters with α = 5% (see Supplementary Fig. 4 with four clusters). We did not constrain the algorithm for the relative size or shape of the clusters, as we had no a priori expectation about them. The clustering algorithm was run for a minimum of 100 iterations and up to a maximum of 125 iterations. To further control for the quality of the cluster assignment, we discarded any fish community for which the second-best cluster assignment was 50% better than the first one by comparing the degree of affiliation to the clusters75. The clustering was performed using the tclust R package75.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
The data used in the study are open access, although we provide the raw data to facilitate the reproduction of the analysis (https://doi.org/10.5281/zenodo.7817360).
Code availability
The manuscript and the Supplementary Information are written in R Markdown, that is, combining code and text, and are available on GitHub (https://github.com/alaindanet/RivFishTimeBiodiversityFacets). We further implemented a code pipeline using the targets R package to ensure that the code, data, figures, manuscript and results are up to date.
References
Zavaleta, E. et al. Ecosystem responses to community disassembly. Ann. NY Acad. Sci. 1162, 311–333 (2009).
Dornelas, M. et al. Assemblage time series reveal biodiversity change but not systematic loss. Science 344, 296–299 (2014).
Blowes, S. A. et al. The geography of biodiversity change in marine and terrestrial assemblages. Science 366, 339–345 (2019).
Primack, R. B. et al. Biodiversity gains? The debate on changes in local- vs global-scale species richness. Biol. Conserv. 219, A1–A3 (2018).
Moore, J. W. & Olden, J. D. Response diversity, nonnative species, and disassembly rules buffer freshwater ecosystem processes from anthropogenic change. Glob. Change Biol. 23, 1871–1880 (2017).
McGill, B. J., Dornelas, M., Gotelli, N. J. & Magurran, A. E. Fifteen forms of biodiversity trend in the Anthropocene. Trends Ecol. Evol. 30, 104–113 (2015).
Tickner, D. et al. Bending the curve of global freshwater biodiversity loss: an emergency recovery plan. BioScience 70, 330–342 (2020).
Reid, A. J. et al. Emerging threats and persistent conservation challenges for freshwater biodiversity. Biol. Rev. 94, 849–873 (2019).
Chen, K. & Olden, J. D. Threshold responses of riverine fish communities to land use conversion across regions of the world. Glob. Change Biol. 26, 4952–4965 (2020).
Comte, L., Grantham, T. & Ruhi, A. Human stabilization of river flows is linked with fish invasions across the USA. Glob. Ecol. Biogeogr. 30, 725–737 (2021).
Newbold, T. et al. Global effects of land use on local terrestrial biodiversity. Nature 520, 45–50 (2015).
Gál, B., Szivák, I., Heino, J. & Schmera, D. The effect of urbanization on freshwater macroinvertebrates – knowledge gaps and future research directions. Ecol. Indic. 104, 357–364 (2019).
Tonella, L. H. et al. Importance of feeding strategies on the long-term success of fish invasions. Hydrobiologia 817, 239–252 (2018).
Su, G., Villéger, S. & Brosse, S. Morphological sorting of introduced freshwater fish species within and between donor realms. Glob. Ecol. Biogeogr. 29, 803–813 (2020).
Leprieur, F., Beauchard, O., Blanchet, S., Oberdorff, T. & Brosse, S. Fish invasions in the world’s river systems: when natural processes are blurred by human activities. PLOS Biol. 6, e28 (2008).
Bernery, C. et al. Freshwater fish invasions: a comprehensive review. Annu. Rev. Ecol. Evol. Syst. 53, 427–456 (2022).
Villéger, S., Blanchet, S., Beauchard, O., Oberdorff, T. & Brosse, S. Homogenization patterns of the world’s freshwater fish faunas. Proc. Natl Acad. Sci. USA 108, 18003–18008 (2011).
Gallardo, B., Clavero, M., Sánchez, M. I. & Vilà, M. Global ecological impacts of invasive species in aquatic ecosystems. Glob. Change Biol. 22, 151–163 (2016).
Olden, J. D., Comte, L. & Giam, X. The Homogocene: a research prospectus for the study of biotic homogenisation. NeoBiota 37, 23–36 (2018).
Chapman, D. S. et al. Invasion of freshwater ecosystems is promoted by network connectivity to hotspots of human activity. Glob. Ecol. Biogeogr. 29, 645–655 (2020).
Lockwood, J. L., Cassey, P. & Blackburn, T. The role of propagule pressure in explaining species invasions. Trends Ecol. Evol. 20, 223–228 (2005).
Gallardo, B., Zieritz, A. & Aldridge, D. C. The importance of the human footprint in shaping the global distribution of terrestrial, freshwater and marine invaders. PLOS ONE 10, e0125801 (2015).
Scott, M. C. & Helfman, G. S. Native invasions, homogenization, and the mismeasure of integrity of fish assemblages. Fisheries 26, 6–15 (2001).
Hillebrand, H. et al. Biodiversity change is uncoupled from species richness trends: consequences for conservation and monitoring. J. Appl. Ecol. 55, 169–184 (2018).
Jackson, S. T. & Sax, D. F. Balancing biodiversity in a changing environment: extinction debt, immigration credit and species turnover. Trends Ecol. Evol. 25, 153–160 (2010).
Venter, O. et al. Sixteen years of change in the global terrestrial human footprint and implications for biodiversity conservation. Nat. Commun. 7, 12558 (2016).
Strayer, D. L., Eviner, V. T., Jeschke, J. M. & Pace, M. L. Understanding the long-term effects of species invasions. Trends Ecol. Evol. 21, 645–651 (2006).
Altermatt, F., Seymour, M. & Martinez, N. River network properties shape α-diversity and community similarity patterns of aquatic insect communities across major drainage basins. J. Biogeogr. 40, 2249–2260 (2013).
Comte, L. et al. RivFishTIME: a global database of fish time-series to study global change ecology in riverine systems. Glob. Ecol. Biogeogr. 30, 38–50 (2021).
Venter, O. et al. Global terrestrial human footprint maps for 1993 and 2009. Sci. Data 3, 160067 (2016).
Anas, M. U. M. & Mandrak, N. E. Drivers of native and non-native freshwater fish richness across North America: disentangling the roles of environmental, historical and anthropogenic factors. Glob. Ecol. Biogeogr. 30, 1232–1244 (2021).
Di Marco, M., Venter, O., Possingham, H. P. & Watson, J. E. M. Changes in human footprint drive changes in species extinction risk. Nat. Commun. 9, 4621 (2018).
van Klink, R. et al. Meta-analysis reveals declines in terrestrial but increases in freshwater insect abundances. Science 368, 417–420 (2020).
Mastrandrea, M. D. et al. Guidance Note for Lead Authors of the IPCC Fifth Assessment Report on Consistent Treatment of Uncertainties (IPCC, 2010); https://www.ipcc.ch/site/assets/uploads/2017/08/AR5_Uncertainty_Guidance_Note.pdf
Vellend, M. et al. Global meta-analysis reveals no net change in local-scale plant biodiversity over time. Proc. Natl Acad. Sci. USA 110, 19456–19459 (2013).
Outhwaite, C. L., Gregory, R. D., Chandler, R. E., Collen, B. & Isaac, N. J. B. Complex long-term biodiversity change among invertebrates, bryophytes and lichens. Nat. Ecol. Evol. 4, 384–392 (2020).
van Strien, A. J. et al. Modest recovery of biodiversity in a western European country: the Living Planet Index for the Netherlands. Biol. Conserv. 200, 44–50 (2016).
He, F. et al. The global decline of freshwater megafauna. Glob. Change Biol. 25, 3883–3892 (2019).
Langford, T. E. L., Shaw, P. J., Ferguson, A. J. D. & Howard, S. R. Long-term recovery of macroinvertebrate biota in grossly polluted streams: re-colonisation as a constraint to ecological quality. Ecol. Indic. 9, 1064–1077 (2009).
Outhwaite, C. L., McCann, P. & Newbold, T. Agriculture and climate change are reshaping insect biodiversity worldwide. Nature 605, 97–102 (2022).
Williams, B. A. et al. Change in terrestrial human footprint drives continued loss of intact ecosystems. One Earth 3, 371–382 (2020).
Mouquet, N. & Loreau, M. Community patterns in source–sink metacommunities. Am. Nat. 162, 544–557 (2003).
Byers, J. E. Impact of non-indigenous species on natives enhanced by anthropogenic alteration of selection regimes. Oikos 97, 449–458 (2002).
Olden, J. D., Poff, N. L. & Bestgen, K. R. Life-history strategies predict fish invasions and extirpations in the Colorado River basin. Ecol. Monogr. 76, 25–40 (2006).
Haubrock, P. J., Pilotto, F., Innocenti, G., Cianfanelli, S. & Haase, P. Two centuries for an almost complete community turnover from native to non-native species in a riverine ecosystem. Glob. Change Biol. 27, 606–623 (2021).
Dornelas, M. et al. A balance of winners and losers in the Anthropocene. Ecol. Lett. 22, 847–854 (2019).
Tonkin, J. D. et al. The role of dispersal in river network metacommunities: patterns, processes, and pathways. Freshwat. Biol. 63, 141–163 (2018).
Dias, M. S. et al. Global imprint of historical connectivity on freshwater fish biodiversity. Ecol. Lett. 17, 1130–1140 (2014).
Olden, J. D. et al. Conservation biogeography of freshwater fishes: recent progress and future challenges. Divers. Distrib. 16, 496–513 (2010).
Comte, L., Olden, J. D., Tedesco, P. A., Ruhi, A. & Giam, X. Climate and land-use changes interact to drive long-term reorganization of riverine fish communities globally. Proc. Natl Acad. Sci. USA 118, e2011639118 (2021).
Mu, H. et al. A global record of annual terrestrial human footprint dataset from 2000 to 2018. Sci. Data 9, 176 (2022).
Linke, S. et al. Global hydro-environmental sub-basin and river reach characteristics at high spatial resolution. Sci. Data 6, 283 (2019).
Lessmann, J. et al. Validating anthropogenic threat maps as a tool for assessing river ecological integrity in Andean–Amazon basins. PeerJ 7, e8060 (2019).
Craig, L. S. et al. Meeting the challenge of interacting threats in freshwater ecosystems: a call to scientists and managers. Elementa 5, 72 (2017).
Simmons, B. I. et al. Refocusing multiple stressor research around the targets and scales of ecological impacts. Nat. Ecol. Evol. 5, 1478–1489 (2021).
Vellend, M. et al. Plant biodiversity change across scales during the Anthropocene. Annu. Rev. Plant Biol. 68, 563–586 (2017).
Svenson, T., Dickson, W., Hellberg, J., Moberg, G. & Munthe, N. The Swedish liming programme. Water Air Soil Pollut. 85, 1003–1008 (1995).
Chao, A. & Jost, L. Coverage-based rarefaction and extrapolation: standardizing samples by completeness rather than size. Ecology 93, 2533–2547 (2012).
Hsieh, T. C., Ma, K. H. & Chao, A. iNEXT: an R package for rarefaction and extrapolation of species diversity (Hill numbers). Methods Ecol. Evol. 7, 1451–1456 (2016).
Tedesco, P. A. et al. A global database on freshwater fish species occurrence in drainage basins. Sci. Data 4, 170141 (2017).
Froese, R. et al. FishBase (Fisheries Centre, Univ. British Columbia, 2021).
Baselga, A. & Orme, C. D. L. Betapart: an R package for the study of beta diversity. Methods Ecol. Evol. 3, 808–812 (2012).
Baselga, A., Bonthoux, S. & Balent, G. Temporal beta diversity of bird assemblages in agricultural landscapes: land cover change vs. stochastic processes. PLOS ONE 10, e0127913 (2015).
Robinson, N., Regetz, J. & Guralnick, R. P. EarthEnv-DEM90: a nearly-global, void-free, multi-scale smoothed, 90m digital elevation model from fused ASTER and SRTM data. ISPRS J. Photogramm. Remote Sens. 87, 57–67 (2014).
Kaiser, H. F. The varimax criterion for analytic rotation in factor analysis. Psychometrika 23, 187–200 (1958).
Gelman, A., Hwang, J. & Vehtari, A. Understanding predictive information criteria for Bayesian models. Stat. Comput. 24, 997–1016 (2014).
Vehtari, A., Gelman, A. & Gabry, J. Practical Bayesian model evaluation using leave-one-out cross-validation and WAIC. Stat. Comput. 27, 1413–1432 (2017).
Gelman, A. Scaling regression inputs by dividing by two standard deviations. Stat. Med. 27, 2865–2873 (2008).
Rue, H., Martino, S. & Chopin, N. Approximate Bayesian inference for latent Gaussian models using integrated nested Laplace approximations. J. R. Stat. Soc. B 71, 319–392 (2009).
Rue, H. et al. Bayesian computing with INLA: a review. Annu. Rev. Stat. Appl. 4, 395–421 (2017).
Hyndman, R. J. Computing and graphing highest density regions. Am. Stat. 50, 120–126 (1996).
Nakagawa, S. & Schielzeth, H. A general and simple method for obtaining R2 from generalized linear mixed-effects models. Methods Ecol. Evol. 4, 133–142 (2013).
LaHuis, D. M., Hartman, M. J., Hakoyama, S. & Clark, P. C. Explained variance measures for multilevel models. Organ. Res. Methods 17, 433–451 (2014).
Gelman, A., Goodrich, B., Gabry, J. & Vehtari, A. R-squared for Bayesian regression models. Am. Stat. 73, 307–309 (2019).
Fritz, H., García-Escudero, L. A. & Mayo-Iscar, A. tclust: an R package for a trimming approach to cluster analysis. J. Stat. Softw. 47, 1–26 (2012).
Acknowledgements
We thank J. Näslund for his assistance accessing the data from the long-term Swedish liming programme. We also thank all the monitoring programme crews and researchers for their continuous efforts of data collection and for making these data publicly available; this study would not be possible without their efforts and generosity. In particular, we would like to acknowledge the authors that gave us the permission to use the data or who made their data available for this study (Supplementary Table 1). We further thank S. Bonnamour, C. Fontaine, G. Loïs, M. Mouchet and E. Porcher at the Centre d’Écologie et des Sciences de la Conservation, as well as A. P. Beckerman and T. F. Johnson at the University of Sheffield for their very constructive feedbacks on the study. J.D.O. was supported by the Richard C. and Lois M. Worthington Endowed Professor in Fisheries Management from the School of Aquatic and Fishery Sciences, University of Washington.
Author information
Authors and Affiliations
Contributions
All authors carried out conceptualization and methodology. A.D. carried out software, validation, formal analysis, visualization and wrote the original draft. X.G. and J.D.O. carried out writing—review and editing. L.C. carried out data curation, supervision, project management, funding acquisition and writing—review and editing.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Ecology & Evolution thanks Ignasi Arranz, Dieison Moi and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data
Extended Data Fig. 1 Characteristics of the fish community time series.
a, Distribution of the year of all sampling events. b–d, Distribution of the (b) first year of sampling, (c) time span, and (d) completeness of the time series across sites. e, time series of yearly sampling events. Colours correspond to the biogeographic realms. In (a), (b), and (e), the lines display the year 1993 and 2009. The years 1993 and 2009 correspond to the years of the human footprint index assessments, which were used to quantify past and recent changes in anthropogenic pressures (See Methods).
Extended Data Fig. 2 Covariations between temporal trends estimated at the site level and the characteristics of the time series.
The year span is expressed in number of years, completeness in proportion of years over the entire study period and first year of sampling in year. The temporal trends were estimated with time as sole fixed predictor (See Methods, eq. (3)). The black lines display the regression lines fitted with a Generalized Additive Model (GAM) and the gray area the confidence interval.
Extended Data Fig. 3 Distribution of community temporal trends across sites.
Temporal trends per decade in Jaccard dissimilarity (a) and nestedness (b). The temporal trends were estimated from a hierarchical Bayesian model including time as sole predictor (See Methods). The histograms show the Best Linear Unbiased Predictor (BLUP) estimated at each site. The points depict the average posterior distribution with bars depicting the Bayesian credible intervals at 95%. The dashed lines refer to no temporal trend. N = 46,932 sampling events across 4,476 sites.
Extended Data Fig. 4 Covariation among the community temporal trends and characterization of community trajectories.
(a, b) PCA biplot of the community temporal trends and their cluster assignment where the sites are colored according to their cluster assignment. (c) Boxplots displaying the distribution of the temporal trends by cluster. The center of the box depicts the median while the bounds depicts the 25% and 75% percentile. The whiskers depict the extreme values within 1.5 interquartile range beyond the bounds of the box. (d) Cluster frequencies across the three main biogeographic realms. The ellipses in (a-b) display the 95% intervals around the clusters assuming a Student’s t distribution. The clusters were named according t o their main characteristics. Contrary to Fig. 2 (Main text), we included the sites whose cluster affiliation was uncertain (‘NA’, black color, (N = 641, 14%). NA clusters are well distributed over the PCA (a-b), which is confirmed by the distribution of community metric temporal trends (c). We observe a few more NAs in the Nearctic realm (+5%, that is around 40 sites over 897).
Extended Data Fig. 5 Predictions of community metric changes over time according to past human pressures and recent changes in human pressures using a hierarchical Bayesian model.
Total abundance is expressed in count, species richness in number of species (raw), while non-native abundance, non-native richness, dissimilarity and turnover are expressed in proportion. No pressure, medium pressure, and highly degraded past pressure levels correspond respectively to values of human footprint index for the year 1993 of 2.5 (intact ecosystem), 16.8 (median value in our dataset), and 45.6 (maximum value in the dataset). Recent pressure levels represent change in human footprint index between the years 1993 and 2009 (see Methods for details). The central lines display the mean, while the lower and upper lines display the credible intervals at 95% of the average posterior distribution.
Extended Data Fig. 6 Covariations between ecological drivers used in the hierarchical Bayesian model (longitudinal stream position, past and recent changes in anthropogenic pressures) and time series characteristics (time span in number of years and beginning of the time series in year).
The blue lines display the regression lines fitted with a Generalized Additive Model (GAM) and the gray area the confidence interval. The ecological drivers were largely unrelated to the characteristics of the time series (N = 4,476 sites).
Extended Data Fig. 7 Rotated PCA over the physical and hydrological characteristics of the sites.
PCA axes (Rotated Component, RC) were rotated using the varimax algorithm (See Methods). The first axis is related to the average discharge, Strahler order and the distance from source, and was subsequently used in the statistical modelling as a composite variable intended to summarise the longitudinal stream position from upstream to downstream.
Extended Data Fig. 8 Drivers of temporal change and spatial variation in fish community metrics.
a,b, Effects of anthropogenic pressures and longitudinal (long.) stream position on temporal changes (a) and spatial variation (b) in fish community metrics. See Fig. 3 in the main text for details. We compare coverage corrected species richness (Richness) with raw species richness (Richness (raw)) and Simpson dissimilarity (Dissimilarity) with Jaccard dissimilarity (Jaccard dissimilarity). We observe that using coverage based species richness or raw species richness does not affect the results. Similarly, we observe consistent effect sizes for Simpson and Jaccard dissimilarity indices, meaning that observed changes in community composition concerned abundant species and thus changes in the identity of dominant species (see Methods). The points depict the average posterior distribution. Large, medium and thin bars depict the Bayesian credible intervals at 80, 90 and 95%, respectively. Please note the broken abscissa scale in (a). N = 46,932 sampling events across 4,476 sites.
Supplementary information
Supplementary Information
Supplementary Figs. 1–4 and Tables 1–5.
Supplementary Software 1
HTML map displaying the temporal trends of the community metrics, cluster assignment and anthropogenic pressures for each site.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Danet, A., Giam, X., Olden, J.D. et al. Past and recent anthropogenic pressures drive rapid changes in riverine fish communities. Nat Ecol Evol 8, 442–453 (2024). https://doi.org/10.1038/s41559-023-02271-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41559-023-02271-x
This article is cited by
-
Under the hood of trends in riverine fish
Nature Ecology & Evolution (2024)