Ribonucleoprotein granules are ubiquitous in living organisms with the protein and RNA components having distinct roles. In the absence of proteins, RNAs are shown to undergo phase separation upon heating. This transition is driven by desolvation entropy and ion-mediated crosslinking and is tuned by the chemical specificity of the RNA nucleobases.
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References
Jain, A. & Vale, R. D. RNA phase transitions in repeat expansion disorders. Nature 546, 243–247 (2017). This paper reports that CG-rich RNAs involved in repeat expansion disorders form neurotoxic foci in cellulo and condensates in vitro.
Van Treeck, B. et al. RNA self-assembly contributes to stress granule formation and defining the stress granule transcriptome. Proc. Natl Acad. Sci. USA 115, 2734–2739 (2018). This paper reports that RNA homopolymers self-assemble in vitro.
Pullara, P., Alshareedah, I. & Banerjee, P. R. Temperature-dependent reentrant phase transition of RNA–polycation mixtures. Soft Matter 18, 1342–1349 (2022). This paper presents data demonstrating the use of temperature-controlled microscopy to observe the closed-loop phase behaviour of poly(rU) in the presence of polyvalent ions.
Mittag, T. & Pappu, R. V. A conceptual framework for understanding phase separation and addressing open questions and challenges. Mol. Cell 82, 2201–2214 (2022). A perspective article that presents the concepts behind the coupling of phase separation and percolation.
Roden, C. & Gladfelter, A. S. RNA contributions to the form and function of biomolecular condensates. Nat. Rev. Mol. Cell Biol. 22, 183–195 (2021). A review article that presents the role of RNA in biomolecular condensates with a focus on RNA properties such as sequence, modifications and length.
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This is a summary of: Wadsworth, G. M. et al. RNAs undergo phase transitions with lower critical solution temperatures. Nat. Chem. https://doi.org/10.1038/s41557-023-01353-4 (2023).
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Phase-separation behaviour of RNAs. Nat. Chem. 15, 1660–1661 (2023). https://doi.org/10.1038/s41557-023-01359-y
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DOI: https://doi.org/10.1038/s41557-023-01359-y
