Addendum: Synthesis and reactivity of precolibactin 886

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Addendum to: Nature Chemistry https://doi.org/10.1038/s41557-019-0338-2, published online 23 September 2019.

On reinspection of the analytical data published in their manuscript, the authors have determined that synthetic precolibactin 886 is produced as a 1:1 mixture of diastereomers (shown in Supplementary Figure 9, which has been added to the Supplementary Information file). The original 1:1.9 ratio of diastereomers reported in the manuscript reflects inadvertent enrichment of the sample following semi-preparative HPLC purification. The authors also found that natural precolibactin 886 is formed as a 1:1 mixture of diastereomers. See Supplementary Figure 5a in Li, Z.-R. et al. Macrocyclic colibactin induces DNA double-strand breaks via copper-mediated oxidative cleavage. Nat. Chem. 11, 880–889 (2019).

Supplementary Fig. 9

Analytical mass-selected LC/HRMS chromatogram of synthetic precolibactin 886 (1) prior to purification.