STEM CELLS

Engineering human knock-in organoids

The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids.

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Fig. 1: Workflow and cellular applications of CRISPR–HOT in ASC-derived organoids.

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Correspondence to Prisca Liberali.

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Yang, Q., Oost, K.C. & Liberali, P. Engineering human knock-in organoids. Nat Cell Biol 22, 261–263 (2020). https://doi.org/10.1038/s41556-020-0478-z

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