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Haematopoiesis in the era of advanced single-cell technologies

Nature Cell Biology volume 21, pages 2–8 (2019)Cite this article

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Abstract

The molecular and functional characterization of single cells at scale has emerged as a key driver to unravel tissue biology. Thus, it is important to understand the strengths and limitations of transcriptomic approaches, molecular barcoding and functional assays used to study cellular properties at the single-cell level. Here, we review recent relevant work from the haematopoietic system and discuss how to interpret and integrate data obtained with different technologies.

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Fig. 1: Alternative hierarchical models of haematopoiesis.
Fig. 2: Possible scenarios underlying in vivo fate mapping outcomes.

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Acknowledgements

We thank S. Orkin and S. Morrison for critical reading of the manuscript and P. Woll for figure preparation. Research in the Jacobsen and Nerlov laboratories is supported by Bloodwise, MRC, BBSRC, the Swedish Research Council, the Knut and Alice Wallenberg Foundation and the Tobias Foundation.

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Authors and Affiliations

  1. MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK

    Sten Eirik W. Jacobsen & Claus Nerlov

  2. Haematopoietic Stem Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK

    Sten Eirik W. Jacobsen

  3. Department of Cell and Molecular Biology, Wallenberg Institute for Regenerative Medicine, Karolinska Institutet, Stockholm, Sweden

    Sten Eirik W. Jacobsen

  4. Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Stockholm, Sweden

    Sten Eirik W. Jacobsen

  5. Karolinska University Hospital, Stockholm, Sweden

    Sten Eirik W. Jacobsen

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  1. Sten Eirik W. Jacobsen
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S.E.W.J. and C.N. contributed equally to the writing and editing of the manuscript as well as to figure preparation.

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Correspondence to Sten Eirik W. Jacobsen or Claus Nerlov.

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Jacobsen, S.E.W., Nerlov, C. Haematopoiesis in the era of advanced single-cell technologies. Nat Cell Biol 21, 2–8 (2019). https://doi.org/10.1038/s41556-018-0227-8

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