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Genome editing

A gentler yield of ex vivo-edited T cells

Nature Biomedical Engineering volume 7, pages 607–608 (2023)Cite this article

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Amphiphilic peptides can aid the delivery of CRISPR ribonucleoproteins into primary human lymphocytes at low toxicity, boosting editing yields with respect to the use of electroporation.

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Fig. 1: Peptide-aided delivery of CRISPR RNPs into lymphocytes.

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Authors and Affiliations

  1. Department of Medicine I: Cardiology, Klinikum rechts der Isar of the Technical University of Munich, TUM School of Medicine and Health, Munich, Germany

    Julian Grünewald

  2. Department of Medicine III: Hematology/Oncology, Klinikum rechts der Isar of the Technical University of Munich, TUM School of Medicine and Health, Munich, Germany

    Andrea Schmidts

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  1. Julian Grünewald
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  2. Andrea Schmidts
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Correspondence to Andrea Schmidts.

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Competing interests

J.G. is a co-inventor on various patent applications filed by Mass General Brigham and the Massachusetts General Hospital on gene-editing technologies. J.G. is a paid consultant for Poseida Therapeutics, a company developing various gene and cell therapies, including CAR T cell therapies and delivery modalities, and has financial interests in the company. A.S. is a contributor to patents related to CAR T cells.

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Grünewald, J., Schmidts, A. A gentler yield of ex vivo-edited T cells. Nat. Biomed. Eng 7, 607–608 (2023). https://doi.org/10.1038/s41551-023-01042-0

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