Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Genome editing

Ablating all mitochondrial protein-coding genes

Nature Biomedical Engineering volume 7, pages 609–611 (2023)Cite this article

Subjects

Each of the 13 protein-coding genes in the mouse mitochondrial genome can be ablated using a library of optimized double-stranded-DNA deaminase-derived cytosine base editors.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Design and optimization of a library of mitochondrial base editors that can knock out each of the 13 protein-coding genes in the mouse mitochondrial genome.

References

  1. Mok, B. Y. et al. Nature 583, 631–637 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Lee, H. et al. Nat. Commun. 12, 1190 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Guo, J. et al. Mol. Ther. Nucleic Acids 27, 73–80 (2022).

    Article  CAS  PubMed  Google Scholar 

  4. Qi, X. et al. Cell Discov. 7, 95 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Guo, J. et al. Cell Discov. 7, 78 (2021).

    PubMed  PubMed Central  Google Scholar 

  6. Lei, Z. et al. Nature 606, 804–811 (2022).

    Article  CAS  PubMed  Google Scholar 

  7. Wei, Y. et al. Cell Discov. 8, 27 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Silva-Pinheiro, P. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-022-00968-1 (2022).

  9. Liu, Z. et al. Sci. Rep. 7, 2193 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Lee, S. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01486-w (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  11. Kozak, M. Nucleic Acids Res. 29, 5226–5232 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Gorman, G. S. et al. Nat. Rev. Dis. Primers 2, 16080 (2016).

    Article  PubMed  Google Scholar 

  13. Lee, S. et al. Genome Biol. 23, 211 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zhejiang Laboratory, Hangzhou, China

    Xin Lou

  2. State Key Laboratory of Reproductive Medicine, Center for Global Health, Gusu School, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China

    Bin Shen

Authors
  1. Xin Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xin Lou or Bin Shen.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lou, X., Shen, B. Ablating all mitochondrial protein-coding genes. Nat. Biomed. Eng 7, 609–611 (2023). https://doi.org/10.1038/s41551-022-00993-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-022-00993-0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research