Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Using extracellular vesicles in blood to diagnose paediatric tuberculosis

Multiplex detection of two interacting Mycobacterium tuberculosis biomarkers on the surface of circulating extracellular vesicles, using a nanoplasmon-enhanced immunoassay, improves the diagnosis of tuberculosis in immunosuppressed children living with HIV.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Schematic of a NEI for diagnosing TB.

References

  1. Dodd, P. J. et al. The global burden of tuberculosis mortality in children: a mathematical modelling study. Lancet Glob. Health 5, e898–e906 (2017). This review discusses the challenges and importance of diagnosing paediatric TB.

    Google Scholar 

  2. Njuguna, I. N. et al. Urgent versus post-stabilisation antiretroviral treatment in hospitalised HIV-infected children in Kenya (PUSH): a randomised controlled trial. Lancet HIV 5, e12–e22 (2018). This report describes the diagnostically challenging cohort of paediatric patients with TB analysed in this study.

    Article  Google Scholar 

  3. Liu, C. et al. Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring. Proc. Natl Acad. Sci. USA 114, 3969–3974 (2017). This study indicates the potential of Mtb-derived serum biomarkers to diagnose TB, using a sophisticated immunoassay that is read by mass spectrometry.

    CAS  Article  Google Scholar 

  4. Gaur, R. L. et al. LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis. PLoS Pathog. 10, e1004376 (2014). This article indicates how the two biomarkers used in our assay interact to mediate Mtb virulence.

    Article  Google Scholar 

  5. Schorey, J. S., Cheng, Y. & McManus, W. R. Bacteria- and host-derived extracellular vesicles—two sides of the same coin? J. Cell Sci. 134, jcs256628 (2021). This review reports that EVs from Mtb-infected macrophages can carry Mtb-derived factors and enter the circulatory system.

    CAS  Article  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Zheng, W. et al. Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-022-00922-1 (2022).

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Using extracellular vesicles in blood to diagnose paediatric tuberculosis. Nat. Biomed. Eng 6, 930–931 (2022). https://doi.org/10.1038/s41551-022-00933-y

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-022-00933-y

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing