Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

IMMUNOENGINEERING

Powered and controlled T-cell production

Nature Biomedical Engineering volume 2, pages 148–150 (2018)Cite this article

Subjects

T cells can be efficiently and controllably expanded for adoptive cell therapy in a culture system consisting of lipid bilayers bearing membrane-bound cues for the stimulation of T-cell receptors and of supporting silica microrods that release a T-cell proliferation factor.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: A scaffold for the efficient ex vivo expansion of T cells.
Fig. 2: Overview of the ex vivo manufacturing process of chimeric antigen receptor T (CAR-T) cells.

References

  1. Carrel, A. & Burrows, M. J. Am. Med. Assoc. 55, 1379–1381 (1910).

    Article  Google Scholar 

  2. Harrison, R. G., Greenman, M. J., Mall, F. P. & Jackson, C. M. Anatom. Rec. 1, 116–128 (1907).

    Article  Google Scholar 

  3. Fesnak, A. D., June, C. H. & Levine, B. L. Nat. Rev. Cancer 16, 566–581 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Cheung, A. S., Zhang, D. K. Y., Koshy, S. T. & Mooney, D. J. Nat. Biotechnol. https://doi.org/10.1038/nbt.4047 (2018).

    PubMed  Google Scholar 

  5. Levine, B. L. et al. J. Hematother. 7, 437–448 (1998).

    Article  CAS  PubMed  Google Scholar 

  6. Kevlahan, S. H., Ball, A., Qin, G., Wells, S. B. & Jesuraj, N. J. Methods and compositions for activation or expansion of T lymphocytes. US patent 9,790,467 (Google Patents, 2017).

  7. Suhoski, M. M. et al. Mol. Ther. 15, 981–988 (2007).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Shibuya, T. Y. et al. Arch. Otolaryngol. Head. Neck Surg. 125, 1229–1234 (1999).

    Article  CAS  PubMed  Google Scholar 

  9. Geppert, T. D. & Lipsky, P. E. J. Immunol. 138, 1660–1666 (1987).

    CAS  PubMed  Google Scholar 

  10. Ma, Z., Discher, D. E. & Finkel, T. H. Front. Immunol. 3, 217 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Comrie, W. A., Li, S., Boyle, S. & Burkhardt, J. K. J. Cell Biol. 208, 457–473 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Bashour, K. T. et al. Mol. Cell Biol. 34, 955–964 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  13. O’Connor, R. S. et al. J. Immunol. 189, 1330–1339 (2012).

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kim, J. et al. Nat. Biotechnol. 33, 64–72 (2015).

    Article  CAS  PubMed  Google Scholar 

  15. Levine, B. L. Cancer Gene Ther. 22, 79–84 (2015).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Cellular Immunotherapies and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Michael C. Milone & Bruce L. Levine

Authors
  1. Michael C. Milone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bruce L. Levine
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bruce L. Levine.

Ethics declarations

Competing interests

M.C.M. and B.L.L. have commercial research grants from Novartis Pharmaceuticals, have ownership interest in intellectual property and patents held by the University of Pennsylvania, and are co-founders of Tycho Therapeutics (M.C.M.) and Tmunity Therapeutics (B.L.L.). These relationships are managed in accordance with University of Pennsylvania policies and oversight.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Milone, M.C., Levine, B.L. Powered and controlled T-cell production. Nat Biomed Eng 2, 148–150 (2018). https://doi.org/10.1038/s41551-018-0206-1

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-018-0206-1

This article is cited by

Search

Advanced search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research